Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 883,414 Δ1 bp intergenic (‑26/‑259) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913883,4110A.97.8% 388.8 / 1.5 90intergenic (‑23/‑262)ybjG/mdfAundecaprenyl pyrophosphate phosphatase/multidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base . (41/47);  total (41/49)
Fisher's exact test for biased strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

AACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTAC  >  NC_000913/883312‑883505
                                                                                                   |                                                                                              
aaCGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTa                                                                                                <  2:338480/100‑1 (MQ=255)
   gagaTCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTaaag                                                                                             <  1:623466/100‑2 (MQ=255)
    agaTCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTaaagg                                                                                            <  2:2424010/100‑3 (MQ=255)
     gaTCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAggg                                                                                           <  1:2964609/100‑1 (MQ=255)
      atcatcCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAggga                                                                                          <  2:1224125/100‑2 (MQ=255)
       tcatcCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAg                                                                                        <  1:187048/100‑1 (MQ=255)
       tcatcCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAg                                                                                        <  1:2527038/100‑1 (MQ=255)
        catcCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAg                                                                                        >  2:228569/1‑99 (MQ=255)
         atcCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGcc                                                                                      <  2:1703086/100‑1 (MQ=255)
         atcCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGcc                                                                                      <  2:1409548/100‑1 (MQ=255)
          tcCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCa                                                                                     >  1:1131802/1‑100 (MQ=255)
          tcCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCa                                                                                     <  2:1725888/100‑1 (MQ=255)
          tcCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCa                                                                                     >  1:92066/1‑100 (MQ=255)
           ccACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCaa                                                                                    <  1:1328706/100‑1 (MQ=255)
              cGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATAc                                                                                 <  2:1131792/100‑1 (MQ=255)
                    cGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCaa                                                                           >  2:7062/1‑100 (MQ=255)
                    cGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCaa                                                                           >  2:2748781/1‑100 (MQ=255)
                    cGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCaa                                                                           >  1:692545/1‑100 (MQ=255)
                    cGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCa                                                                            <  2:825508/99‑1 (MQ=255)
                     gAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAg                                                                          <  1:2958064/100‑1 (MQ=255)
                     gAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAg                                                                          <  1:2632929/100‑1 (MQ=255)
                      aGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGt                                                                         >  1:263524/1‑100 (MQ=255)
                       gTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTc                                                                        <  1:2974485/100‑1 (MQ=255)
                          tGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGtt                                                                     >  2:869818/1‑100 (MQ=255)
                           ggCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTg                                                                    >  1:2113136/1‑100 (MQ=255)
                           ggCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTg                                                                    <  2:2722300/100‑1 (MQ=255)
                            gCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTga                                                                   >  2:920470/1‑100 (MQ=255)
                             cGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTgag                                                                  <  2:2169252/100‑1 (MQ=255)
                              gTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTgaga                                                                 <  2:1490140/100‑1 (MQ=255)
                                cgcgTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAAt                                                               >  1:2743319/1‑100 (MQ=255)
                                 gcgTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAAt                                                               >  1:2532421/1‑99 (MQ=255)
                                 gcgTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATa                                                              <  2:1611607/100‑1 (MQ=255)
                                  cgTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATaa                                                             >  2:1266755/1‑100 (MQ=255)
                                  cgTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATaa                                                             <  1:1799857/100‑1 (MQ=255)
                                  cgTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGAGATTAAAGGGAGCCAATACAGGCAAGTCGTGGAGAAt                                                               >  2:2149084/1‑98 (MQ=255)
                                  cgTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTACAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATaa                                                             >  1:2141834/1‑100 (MQ=255)
                                     taataaGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGt                                                          <  2:229689/100‑1 (MQ=255)
                                     taataaGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGt                                                          >  2:2374302/1‑100 (MQ=255)
                                         aaGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAg                                                      >  1:2045430/1‑100 (MQ=255)
                                         aaGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAg                                                      >  1:229639/1‑100 (MQ=255)
                                         aaGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAg                                                      <  2:1468910/100‑1 (MQ=255)
                                         aaGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAg                                                      <  2:1468839/100‑1 (MQ=255)
                                         aaGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAg                                                      >  1:2046035/1‑100 (MQ=255)
                                         aaGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGAAGCCATTACAGCCAAGCCGTTGAGATTAAAGTGCAg                                                      >  2:406588/1‑100 (MQ=255)
                                          agagagAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAgg                                                     <  1:2012214/100‑1 (MQ=255)
                                          agagagAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAgg                                                     >  1:2304854/1‑100 (MQ=255)
                                          agagagAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAgg                                                     >  1:1384626/1‑100 (MQ=255)
                                          agagagAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAgg                                                     >  2:343776/1‑100 (MQ=255)
                                           gagagaATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGt                                                    >  1:2576498/1‑100 (MQ=255)
                                            agagaATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGtt                                                   >  2:2477479/1‑100 (MQ=255)
                                             gagaATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTa                                                  <  1:7062/100‑1 (MQ=255)
                                                    gagagagaTTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGGATAAAGTGCAGGTTAAACTggg                                           <  2:2797928/100‑1 (MQ=255)
                                                    gagagagaTTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTggg                                           <  1:47718/100‑1 (MQ=255)
                                                    gagagagaTTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTggg                                           <  2:2430273/100‑1 (MQ=255)
                                                    gagagagaTTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTggg                                           <  1:869826/100‑1 (MQ=255)
                                                    gagagagaTTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTggg                                           <  1:1673959/100‑1 (MQ=255)
                                                    gagagagaTTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTggg                                           <  2:1919429/100‑1 (MQ=255)
                                                    gagagagaTTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTggg                                           >  1:352861/1‑100 (MQ=255)
                                                      gagagaTTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTa                                         >  1:2891346/1‑100 (MQ=255)
                                                      gagagaTTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTa                                         >  2:1723375/1‑100 (MQ=255)
                                                       agagaTTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGAGAGCCAATACAGGCAAGTAGTTGAGAATAAAGTGCAGGTTAAACTGGGTaa                                        >  2:1072798/1‑100 (MQ=255)
                                                          gaTTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGc                                     >  2:2066657/1‑100 (MQ=255)
                                                            ttCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCgg                                   >  2:118290/1‑100 (MQ=255)
                                                            ttCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCgg                                   <  1:2748805/100‑1 (MQ=255)
                                                            ttCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCgg                                   <  1:405729/100‑1 (MQ=255)
                                                            tccAAATTTTCCAACATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCgg                                   >  1:2039960/3‑100 (MQ=255)
                                                              cAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCa                                 >  2:725113/1‑100 (MQ=255)
                                                               aaaTTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGCGAATAAAGTGCAGGTTAAACTGGGTCACGCGGCAt                                >  1:1768595/1‑100 (MQ=255)
                                                               aaaTTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCAt                                >  2:34266/1‑100 (MQ=255)
                                                               aaaTTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCAt                                >  1:2551674/1‑100 (MQ=255)
                                                               aaaTTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCAt                                >  2:815829/1‑100 (MQ=255)
                                                               aaaTTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCAt                                <  2:2532398/100‑1 (MQ=255)
                                                               aaaTTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCAt                                <  1:1763814/100‑1 (MQ=255)
                                                                    ttCCAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCt                           <  1:1559036/100‑1 (MQ=255)
                                                                       cAGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTAt                        <  2:1696974/100‑1 (MQ=255)
                                                                       cAGCATAACTGCCCGACGCGAAGTGATTAAAGGGAGCCAATACAGGCAAGGCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTAt                        <  2:800744/100‑1 (MQ=255)
                                                                        aGCATAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTAtt                       >  1:2593818/1‑100 (MQ=255)
                                                                            tAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTccc                   <  2:2810751/100‑1 (MQ=255)
                                                                            tAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTccc                   >  1:919899/1‑100 (MQ=255)
                                                                            tAACTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTccc                   <  1:1127117/100‑1 (MQ=255)
                                                                             aaCTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCt                  >  2:1548611/1‑100 (MQ=255)
                                                                               cTTCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCa                <  2:1861964/100‑1 (MQ=255)
                                                                                 tCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAg              <  1:2411629/100‑1 (MQ=255)
                                                                                 tCCCGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAg              <  1:62153/100‑1 (MQ=255)
                                                                                   ccGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCg            <  1:1511856/100‑1 (MQ=255)
                                                                                    cGACGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCgg           >  2:2169731/1‑100 (MQ=255)
                                                                                      aCGCAAAGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGcc         <  2:1609348/100‑1 (MQ=255)
                                                                                          aaaGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGtt     <  2:2046017/100‑1 (MQ=255)
                                                                                          aaaGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGtt     <  2:2045412/100‑1 (MQ=255)
                                                                                          aaaGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGtt     <  2:1518978/100‑1 (MQ=255)
                                                                                          aaaGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGtt     <  2:1198120/100‑1 (MQ=255)
                                                                                            aGTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTa   <  1:174850/100‑1 (MQ=255)
                                                                                             gTGATTAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTAc  <  1:2169750/100‑1 (MQ=255)
                                                                                                   |                                                                                              
AACGAGATCATCCACGGAGCCGAGTCTGGCGTCGCGTTAATAAGAGAGAATAGAGAGAGATTCAAATTTTCCAGCATAACTTCCCGACGCAAAGTGATTAAAAGGGAGCCAATACAGGCAAGTCGTTGAGAATAAAGTGCAGGTTAAACTGGGTAAAGCGGCATCGTCTTATTTCCCTCAAGCGGCCTGTTTAC  >  NC_000913/883312‑883505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: