Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,469,733 (T)8→7 coding (603/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,469,7260T.93.1% 504.8 / 30.8 131coding (596/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base T (6/3);  new base . (55/67);  total (61/70)
Fisher's exact test for biased strand distribution p-value = 3.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.90e-01

Only 100 of 150 total aligned reads displayed.

ACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTT  >  NC_000913/2469627‑2469820
                                                                                                   |                                                                                              
  aaTAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttt                                                                                              >  1:1554661/1‑100 (MQ=255)
     aaaTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttt                                                                                           <  2:1634441/100‑1 (MQ=255)
      aaTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt                                                                                          <  1:633506/100‑1 (MQ=255)
      aaTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt                                                                                          <  2:2290247/100‑1 (MQ=255)
      aaTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt                                                                                          <  2:901587/100‑1 (MQ=255)
       aTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAGCCCCACTAATAGTGTATTCATATTATAtttttttg                                                                                         <  1:2713837/100‑2 (MQ=255)
           aTGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAg                                                                                    >  1:2307706/1‑100 (MQ=255)
           aTGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAg                                                                                    <  1:1775269/100‑1 (MQ=255)
             gCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGac                                                                                  <  1:86442/100‑1 (MQ=255)
              cTTTTGCTATTTTTTTTTTTTTTTTTAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttttta                                                                                      >  2:75111/1‑93 (MQ=255)
               ttttGCTATTTTTTTCGTTGCTAATCCTCTCTACTGCTAAATATCACTTTATCTTAACCCCACTAATAGTTTATTCATATTATATTTTTTTGATAGacac                                                                                <  2:557085/100‑1 (MQ=255)
                tttGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGAcaca                                                                               <  1:28989/100‑1 (MQ=255)
                  tGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACaaa                                                                             <  1:1295682/100‑1 (MQ=255)
                   gCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAAc                                                                            >  2:1724871/1‑100 (MQ=255)
                   gCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAAc                                                                            >  2:1797287/1‑100 (MQ=255)
                   gCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAAc                                                                            >  1:379606/1‑100 (MQ=255)
                   gCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAAc                                                                            <  1:553168/100‑1 (MQ=255)
                   gCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAAc                                                                            >  1:2959699/1‑100 (MQ=255)
                    cTATTTTTTTCTTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACt                                                                           >  2:68869/1‑100 (MQ=255)
                     tATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTa                                                                          >  2:474301/1‑100 (MQ=255)
                      aTTTTTTTCGTGGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAGTAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTa                                                                          >  2:53577/1‑100 (MQ=255)
                         tttttCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAatta                                                                      <  1:101399/100‑1 (MQ=255)
                           tttCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAattatt                                                                    <  2:379601/100‑1 (MQ=255)
                            ttCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAAttatta                                                                   >  1:415356/1‑100 (MQ=255)
                              cGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTaa                                                                  <  2:1975843/100‑1 (MQ=255)
                              cGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTaaa                                                                 >  1:929007/1‑100 (MQ=255)
                              cGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTaaa                                                                 >  1:1035101/1‑100 (MQ=255)
                              cGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTaaa                                                                 >  2:1392093/1‑100 (MQ=255)
                              cGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTaaa                                                                 >  2:2662385/1‑100 (MQ=255)
                               gTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAAt                                                                >  2:1437634/1‑100 (MQ=255)
                               gTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAAt                                                                >  2:3009298/1‑100 (MQ=255)
                               gTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAAt                                                                >  1:502214/1‑100 (MQ=255)
                                 tGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCt                                                              >  1:49115/1‑100 (MQ=255)
                                   cTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGt                                                            <  2:2460230/100‑1 (MQ=255)
                                    taataaTATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGt                                                            >  2:887383/1‑99 (MQ=255)
                                    taataaTATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTa                                                           <  1:32738/100‑1 (MQ=255)
                                      ataataTCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAat                                                         <  2:623621/100‑1 (MQ=255)
                                      ataataTCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAat                                                         <  1:297127/100‑1 (MQ=255)
                                        aataTCTACTNCTNAANATNAANTTATATTNACNCCACNAANAGTGNATTCNTNTTATATNTTTTTGATNGACACAAACTAATTATTAAATCTGTAanat                                                       >  2:2446947/1‑100 (MQ=255)
                                        aataTCTACTGCTAAAAATCAATTTATATTAACCCCTCTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAatat                                                       <  2:2569450/100‑1 (MQ=255)
                                          tatCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGc                                                     <  2:22587/100‑1 (MQ=255)
                                            tCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgt                                                   >  1:2871934/1‑100 (MQ=255)
                                             cTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgtg                                                  <  2:811019/100‑1 (MQ=255)
                                              tACGGCTAAAAATCAATTTATATTAACCCCACTATTAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgtg                                                  <  1:1157646/99‑1 (MQ=255)
                                                cTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgtggtg                                               >  2:3009077/1‑100 (MQ=255)
                                                cTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgtggtg                                               <  2:1035091/100‑1 (MQ=255)
                                                  gCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATTCGTGgtgt                                              >  2:294832/1‑99 (MQ=255)
                                                  gCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGtgtg                                             >  1:2171626/1‑100 (MQ=255)
                                                   cTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgc                                            >  2:2199098/1‑100 (MQ=255)
                                                   cTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgc                                            <  2:1524272/100‑1 (MQ=255)
                                                   cTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgc                                            >  1:181992/1‑100 (MQ=255)
                                                   cTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgc                                            >  2:856107/1‑100 (MQ=255)
                                                    tAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgct                                           >  1:2388017/1‑100 (MQ=255)
                                                    tAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgct                                           >  2:381648/1‑100 (MQ=255)
                                                     aaaaaTCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgctt                                          <  1:588462/100‑1 (MQ=255)
                                                     aaaaaTCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgctt                                          >  2:1018158/1‑100 (MQ=255)
                                                     aaaaaTCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgctt                                          <  1:1237391/100‑1 (MQ=255)
                                                      aaaaTCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgcttg                                         <  2:1250358/100‑1 (MQ=255)
                                                           cAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCttgcttgc                                    <  1:2401263/100‑1 (MQ=255)
                                                            aaTTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCg                                   <  1:2845709/100‑1 (MQ=255)
                                                            aaTTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCg                                   <  1:1620788/100‑1 (MQ=255)
                                                             aTTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTTCTTGCTTGCGt                                  >  1:1931565/1‑100 (MQ=255)
                                                             aTTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGt                                  >  2:2371880/1‑100 (MQ=255)
                                                              tttATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTc                                 <  2:263458/100‑1 (MQ=255)
                                                              tttATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTc                                 >  1:1814938/1‑100 (MQ=255)
                                                                tataTTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCaa                               >  1:1300621/1‑100 (MQ=255)
                                                                  tatTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAata                             >  2:1664940/1‑100 (MQ=255)
                                                                   atTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAatat                            >  2:2286327/1‑100 (MQ=255)
                                                                    ttAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATAtt                           <  1:2764182/100‑1 (MQ=255)
                                                                      aaCCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTg                         <  1:75112/100‑1 (MQ=255)
                                                                      aaCCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTg                         <  1:175778/100‑1 (MQ=255)
                                                                       aCCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGc                        <  2:2758431/100‑1 (MQ=255)
                                                                       aCCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGc                        <  2:1300608/100‑1 (MQ=255)
                                                                       aCCCCACTAAGAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAAGATGCGTGGTGTGCTTGCTTGCGTCAATATTTGc                        <  2:1998484/100‑1 (MQ=255)
                                                                        ccccACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCa                       <  2:1984730/100‑1 (MQ=255)
                                                                        ccccACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCa                       <  2:1901978/100‑1 (MQ=255)
                                                                         cccACTAATAGTGTATTCATATTATATTTTTTTTATATACATAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCa                       >  2:1382247/1‑99 (MQ=255)
                                                                          ccACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAat                     >  1:667120/1‑100 (MQ=255)
                                                                              tAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCt                 <  1:331194/100‑1 (MQ=255)
                                                                              tAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAAAATCt                 <  2:444779/100‑1 (MQ=255)
                                                                               aaTAGTGTATTCATATTATATTTTTTTTTTTTTTTTTTAATAATTATTAAGTCTATAATATGCGTGTTTTGCTTGCTTGCGTCAATATTTGCAATATCtt                >  2:950216/1‑100 (MQ=255)
                                                                               aaTAGTGTATTCATATTATATTTTTTTTTTTTTTTTAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCTATATCtt                >  2:949814/1‑100 (MQ=255)
                                                                                aTAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTa               <  1:2538931/100‑1 (MQ=255)
                                                                                 tAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTAt              >  1:1703801/1‑100 (MQ=255)
                                                                                  aGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTAtt             >  2:1734572/1‑100 (MQ=255)
                                                                                   tcttATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTc            <  1:2228257/97‑1 (MQ=255)
                                                                                   gtgtATTCATATTATATTTTTTTTTTATATATAAAATAATTATTAAATCTGTAATATGCGTGTTGTGCTTGATTTCGTTAATATTTGCAATAtattat              >  2:2763880/1‑98 (MQ=255)
                                                                                     gtATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCaa          >  1:1871659/1‑100 (MQ=255)
                                                                                     gtATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCaa          >  1:927601/1‑100 (MQ=255)
                                                                                      tATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCaga         >  2:2228237/1‑98 (MQ=255)
                                                                                       aTTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCaaa         <  2:338884/100‑1 (MQ=255)
                                                                                       aTTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCaaaa        <  1:2733357/100‑1 (MQ=255)
                                                                                        ttCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAg       <  2:2307686/100‑1 (MQ=255)
                                                                                         tCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAgg      <  2:2572060/100‑1 (MQ=255)
                                                                                         tCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCATGCTTGCGTCAATATTTGCAATATCTTATTCAAAAgg      >  2:2049847/1‑100 (MQ=255)
                                                                                          cATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGTTGTGATTGTTTGCTTTAATATTTGTATTATCTTAttacc          >  2:2421292/1‑92 (MQ=255)
                                                                                           atattatattTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGgtg    <  1:794085/100‑1 (MQ=255)
                                                                                           atattatattTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGgtg    <  1:856256/100‑1 (MQ=255)
                                                                                            tattatattTTTTTGGTAGCCACAAGATAATTAACAAATTTGTAATATGCTTGGTGTTCTTTCTTGCGTCAATAGTTGCATGATCTTCTTGCAAAGgtgt   >  2:592686/1‑100 (MQ=255)
                                                                                             attatattTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGtgtt  >  1:1717610/1‑100 (MQ=255)
                                                                                                   |                                                                                              
ACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTT  >  NC_000913/2469627‑2469820

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: