Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,470,410 (T)7→6 coding (1280/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,470,4040T.96.7% 646.5 / 13.1 153coding (1274/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base T (4/1);  new base . (55/93);  total (59/94)
Fisher's exact test for biased strand distribution p-value = 7.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.53e-01

Only 100 of 163 total aligned reads displayed.

AATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTA  >  NC_000913/2470305‑2470499
                                                                                                   |                                                                                               
  ttttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttt                                                                                               <  2:1015505/100‑1 (MQ=255)
    ttATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttn                                                                                             <  1:2414388/100‑2 (MQ=255)
     tATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGtttttt                                                                                            <  2:2705061/100‑1 (MQ=255)
       tgtgTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTa                                                                                          <  2:165268/100‑1 (MQ=255)
        gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaat                                                                                         >  2:1611213/1‑97 (MQ=255)
        gtgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTaa                                                                                         >  1:2969767/1‑100 (MQ=255)
         tgtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGttttttaatt                                                                                        <  1:1060370/100‑5 (MQ=255)
          gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAAttt                                                                                      <  1:1516355/100‑1 (MQ=255)
          gtCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAAttt                                                                                      <  1:218778/100‑1 (MQ=255)
           tCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTACTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTta                                                                                     >  2:535845/1‑100 (MQ=255)
              tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATaa                                                                                  >  1:1315605/1‑100 (MQ=255)
              tttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATaa                                                                                  <  1:1697928/100‑1 (MQ=255)
               ttttCGGGGAAGGATATAGAGATTTAAGCAAGTATTTATTTCGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATaaa                                                                                 <  2:1553075/100‑1 (MQ=255)
               ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATaaa                                                                                 >  1:971770/1‑100 (MQ=255)
               ttttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATaaa                                                                                 <  1:2179947/100‑1 (MQ=255)
               ttttAGGGGAAGGATATTGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTTGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTta                                                                                     <  2:2635485/96‑1 (MQ=255)
                 ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAaat                                                                               <  1:1954631/100‑1 (MQ=255)
                 ttCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAaat                                                                               <  1:2254076/100‑1 (MQ=255)
                   cGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTTTTTTGTTTTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAaat                                                                               >  2:1805365/1‑98 (MQ=255)
                    ggggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCCACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAaataat                                                                            <  1:1753086/100‑1 (MQ=255)
                      ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTc                                                                          <  2:1708117/100‑1 (MQ=255)
                      ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTc                                                                          <  2:1339845/100‑1 (MQ=255)
                      ggAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTc                                                                          <  1:2597193/100‑1 (MQ=255)
                         aGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCaaa                                                                       >  2:1683379/1‑100 (MQ=255)
                          ggATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAg                                                                      <  2:1932444/100‑1 (MQ=255)
                           gATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGa                                                                     >  1:1273852/1‑100 (MQ=255)
                            atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAg                                                                      <  2:53182/98‑1 (MQ=255)
                            atatAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGa                                                                     <  2:1415233/99‑1 (MQ=255)
                             tataGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTAGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGaaa                                                                   <  2:78537/100‑1 (MQ=255)
                              ataGATATTTAAGCAATCATTTATTTGGAATGTATTTTTCGTTAGACCTTTACTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAAt                                                                  <  2:298319/100‑1 (MQ=255)
                               taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                 >  1:1312201/1‑100 (MQ=255)
                               taGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                 >  2:34429/1‑100 (MQ=255)
                                agagATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATc                                                                 >  2:2960084/1‑99 (MQ=255)
                                  agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGATCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                              <  2:1420366/100‑1 (MQ=255)
                                  agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCaa                                                               <  2:772902/99‑1 (MQ=255)
                                  agaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                              <  1:1110432/100‑1 (MQ=255)
                                   gaTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAg                                                              <  2:1636977/99‑1 (MQ=255)
                                    aTTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAc                                                            <  2:1707556/100‑1 (MQ=255)
                                      ttAAGCCATCATTTATTTGGAATGTATTTTTCGTTAGACCTTTACTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGa                                                             <  2:3011210/97‑1 (MQ=255)
                                       tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGAc                                                            <  1:2941958/97‑1 (MQ=255)
                                       tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAt                                                         >  1:1136685/1‑100 (MQ=255)
                                       tAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAAt                                                         >  1:2308804/1‑100 (MQ=255)
                                         agcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                       <  1:427304/100‑1 (MQ=255)
                                          gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTTGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                       >  1:2170616/1‑99 (MQ=255)
                                          gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAgn                                                      <  1:2403838/100‑2 (MQ=255)
                                          gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGc                                                      >  1:1473770/1‑100 (MQ=255)
                                          gcaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGc                                                      <  2:2767843/100‑1 (MQ=255)
                                          gaaagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAg                                                       <  1:627011/97‑1 (MQ=255)
                                           caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                     >  1:672416/1‑100 (MQ=255)
                                           caagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCg                                                     <  1:1685481/100‑1 (MQ=255)
                                            aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                    <  1:768839/100‑1 (MQ=255)
                                            aagcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGa                                                    <  2:25457/100‑1 (MQ=255)
                                              tcaTTTATTTGTAATGTGATTTTCGCTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGc                                                      <  2:1722354/95‑1 (MQ=255)
                                              gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATg                                                  <  1:865696/100‑1 (MQ=255)
                                              gcaTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATg                                                  <  1:2053718/100‑1 (MQ=255)
                                              gcaTTTATATAGAACGTATTTTTCGTTCAACCTTTACTTATACATACCAGTCGATTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATCGCGATg                                                  <  2:2883975/100‑1 (MQ=255)
                                               caTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGAt                                                   <  2:2363079/98‑1 (MQ=255)
                                               caTTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGt                                                 <  2:2058590/100‑1 (MQ=255)
                                                atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTa                                                <  2:3019307/100‑1 (MQ=255)
                                                atttatttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTa                                                <  2:2171057/100‑1 (MQ=255)
                                                   tatttGGAATGTATTTTTCGTTCGAACTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAg                                             >  2:1132983/1‑100 (MQ=255)
                                                    atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                            <  2:280228/100‑1 (MQ=255)
                                                    atttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGc                                            <  1:2957183/100‑1 (MQ=255)
                                                     tttGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTGTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCa                                           <  2:2175385/100‑1 (MQ=255)
                                                       tGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACt                                         >  2:2756872/1‑100 (MQ=255)
                                                        ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTa                                        <  2:1312188/100‑1 (MQ=255)
                                                        ggAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTa                                        >  2:1467014/1‑100 (MQ=255)
                                                          aaTGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAg                                      <  1:2548046/100‑1 (MQ=255)
                                                            tGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGtt                                    <  1:1980440/100‑1 (MQ=255)
                                                             gTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAg                                      >  2:721717/1‑97 (MQ=255)
                                                              tATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTa                                  >  2:984876/1‑100 (MQ=255)
                                                               aTTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaa                                 <  1:3011531/100‑1 (MQ=255)
                                                                tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                <  2:1470788/100‑1 (MQ=255)
                                                                tttttCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTaaa                                >  1:2750284/1‑100 (MQ=255)
                                                                     cgttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcg                           <  2:1774060/100‑1 (MQ=255)
                                                                      gttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                          <  1:1578212/100‑1 (MQ=255)
                                                                      gttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgc                          >  1:2891334/1‑100 (MQ=255)
                                                                       ttcgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTgcgcg                         >  1:2471364/1‑100 (MQ=255)
                                                                         cgACCTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGcc                       >  1:2099207/1‑100 (MQ=255)
                                                                            ccTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAAt                    <  1:1622398/100‑1 (MQ=255)
                                                                            ccTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAAt                    >  1:2222070/1‑100 (MQ=255)
                                                                             cTTTGCTTATACATAACAGTCGTTTTTTATTTTTTATAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAAt                    >  2:1377781/1‑99 (MQ=255)
                                                                             cTTTGCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATc                   >  2:2101084/1‑100 (MQ=255)
                                                                                 gCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg               <  2:1643634/100‑1 (MQ=255)
                                                                                 gCTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg               >  1:304100/1‑100 (MQ=255)
                                                                                  cTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATgg               >  2:2888014/1‑99 (MQ=255)
                                                                                  cTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc              >  1:2544436/1‑100 (MQ=255)
                                                                                  cTTATACATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgc              >  1:991314/1‑100 (MQ=255)
                                                                                     ataCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcg           <  1:1603743/100‑1 (MQ=255)
                                                                                      taCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcgc          >  1:236136/1‑100 (MQ=255)
                                                                                      taCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcgc          >  2:317181/1‑100 (MQ=255)
                                                                                      taCATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGgcgcgc          >  1:1546637/1‑100 (MQ=255)
                                                                                       aCATAACAGTCGTTTTTTTTTTTTTTTTTTAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGca         >  2:2371685/1‑100 (MQ=255)
                                                                                        cATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcac        >  1:718360/1‑100 (MQ=255)
                                                                                        cATAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGcac        >  1:1055450/1‑100 (MQ=255)
                                                                                          tAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa      >  1:913442/1‑100 (MQ=255)
                                                                                          tAACAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACaa      >  1:2224677/1‑100 (MQ=255)
                                                                                           aaCAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAg     <  1:607020/100‑1 (MQ=255)
                                                                                            aCAGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTCAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGc    <  1:2137086/100‑1 (MQ=255)
                                                                                              aGTCGTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCta  <  2:2222050/100‑1 (MQ=255)
                                                                                                   |                                                                                               
AATTTTATGTGTCATTTTTCGGGGAAGGATATAGAGATTTAAGCAAGCATTTATTTGGAATGTATTTTTCGTTCGACCTTTGCTTATACATAACAGTCGTTTTTTTAATTTATAAAATAATTCAAAGAAATCAAGACAATAGCGATGTAAAGCACTAAGTTTAAATTGCGCGCCAATCATGGCGCGCACAAGCTA  >  NC_000913/2470305‑2470499

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: