Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 380,021 (G)10→8 intergenic (‑140/+47) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0130G.100.0% 527.1 / NA 116intergenic (‑132/+56)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base . (65/51);  total (65/51)
*NC_000913380,0140G.98.3% 514.4 / 1.3 116intergenic (‑133/+55)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base . (63/51);  total (65/51)
Fisher's exact test for biased strand distribution p-value = 5.03e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

Only 100 of 132 total aligned reads displayed.

AGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAC  >  NC_000913/379915‑380106
                                                                                                  ||                                                                                            
aGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                                                                              >  2:1758389/1‑100 (MQ=255)
aGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                                                                              <  2:2146693/100‑1 (MQ=255)
 ggggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                                                                             >  1:924479/1‑100 (MQ=255)
 ggggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                                                                             >  2:35586/1‑100 (MQ=255)
 ggggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                                                                             >  2:677849/1‑100 (MQ=255)
 ggggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGCCGGAAAACAAAGAGGAAAggg                                                                                             >  2:2964927/1‑100 (MQ=255)
   ggggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggg                                                                                           >  1:643357/1‑100 (MQ=255)
    gggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg                                                                                          >  2:969398/1‑100 (MQ=255)
     ggTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggg                                                                                         <  2:332891/100‑1 (MQ=255)
        aTTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggc                                                                                       <  1:1158977/99‑2 (MQ=255)
           cTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggctaat                                                                                   <  2:402825/100‑6 (MQ=255)
            tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggctaatc                                                                                  >  1:547921/1‑94 (MQ=255)
            tataTGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggctaatcg                                                                                  >  1:2454629/1‑93 (MQ=255)
              tatGTCAATGCATACCCCCCTATAGTATATAACGCTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAGAACAAAGAGGAAA‑‑GGGGGGGGCTAATCgg                                                                              >  2:1824493/1‑100 (MQ=255)
              tatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggggctaatc                                                                                  <  2:1676429/98‑7 (MQ=255)
                tGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCa                                                                            <  2:2052014/100‑1 (MQ=255)
                tGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCa                                                                            >  1:1421647/1‑100 (MQ=255)
                  tCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAgg                                                                          <  1:958367/100‑1 (MQ=255)
                   cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAggg                                                                         >  2:1077516/1‑100 (MQ=255)
                   cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAggg                                                                         <  1:2896881/100‑1 (MQ=255)
                    aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGa                                                                        <  2:2939281/100‑1 (MQ=255)
                    aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGa                                                                        >  2:2677628/1‑100 (MQ=255)
                    aaTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGa                                                                        >  1:2706400/1‑100 (MQ=255)
                    aaTGCATACCCCCCTATAGTATATAACACTGGAAAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGa                                                                        >  2:1038726/1‑100 (MQ=255)
                     aTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGaa                                                                       >  1:2910524/1‑100 (MQ=255)
                     aTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGaa                                                                       >  1:360552/1‑100 (MQ=255)
                     aTGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGaa                                                                       >  1:17952/1‑100 (MQ=255)
                      tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAg                                                                      <  2:2898604/100‑1 (MQ=255)
                      tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAg                                                                      <  2:2204030/100‑1 (MQ=255)
                             cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGccc                                                               >  1:2483763/1‑100 (MQ=255)
                             cccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGccc                                                               >  1:2483677/1‑100 (MQ=255)
                              ccccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGcccc                                                              <  1:2698336/100‑1 (MQ=255)
                                ccTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                            <  2:1889296/100‑1 (MQ=255)
                                  tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAt                                                          >  2:75822/1‑100 (MQ=255)
                                  tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAt                                                          >  2:2871138/1‑100 (MQ=255)
                                  tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAt                                                          <  2:686948/100‑1 (MQ=255)
                                  tataGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAt                                                          <  2:955919/100‑1 (MQ=255)
                                    taGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAg                                                        >  2:617108/1‑100 (MQ=255)
                                     aGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc                                                       <  1:1704311/100‑1 (MQ=255)
                                     aGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgc                                                       <  1:1756674/100‑1 (MQ=255)
                                        atataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                    >  2:1889485/1‑100 (MQ=255)
                                        atataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                    >  1:1500981/1‑100 (MQ=255)
                                        atataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcggg                                                    >  2:1087287/1‑100 (MQ=255)
                                          ataACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgggcg                                                  <  2:95127/100‑1 (MQ=255)
                                             acacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                               >  1:1553635/1‑100 (MQ=255)
                                             acacTGGAGAATAAAATTTATCCGGTGAATGAAGCCGGAAAACAAAGAGGAAA‑‑GGGGGGCGCTAATCGGCAGGGAAGGCCACCCCGGATAGTGGGCGGCa                                               >  2:2005437/1‑100 (MQ=255)
                                              cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAg                                              >  2:2519801/1‑100 (MQ=255)
                                              cacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAg                                              <  2:2483741/100‑1 (MQ=255)
                                               acTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa                                             <  2:2218625/100‑1 (MQ=255)
                                               acTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGa                                             <  2:360547/100‑1 (MQ=255)
                                                 tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                           <  1:1435039/100‑1 (MQ=255)
                                                  ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                          >  2:1995895/1‑100 (MQ=255)
                                                  ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                          >  2:1692986/1‑100 (MQ=255)
                                                  ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                          <  1:676325/100‑1 (MQ=255)
                                                  ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                          <  1:1706203/100‑1 (MQ=255)
                                                     gaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                       <  1:2208789/100‑1 (MQ=255)
                                                     gaATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAt                                       >  1:2843385/1‑100 (MQ=255)
                                                      aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc                                      >  2:1219964/1‑100 (MQ=255)
                                                      aaTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc                                      <  1:2087758/100‑1 (MQ=255)
                                                       aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGACAGAAGGAATCa                                     >  1:1025885/1‑100 (MQ=255)
                                                       aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCAAATCGGCAGGGAACGCCGCCCCGGATAGCGGGCGGCAGAACGAATc                                      <  2:437020/99‑1 (MQ=255)
                                                       aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGATAATCGGGAGGGCAGCCCGCCCAGGATAGAGGGCGGCAGAAGGAATc                                      >  2:123262/1‑99 (MQ=255)
                                                        tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                    >  1:2963235/1‑100 (MQ=255)
                                                        tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                    <  2:740125/100‑1 (MQ=255)
                                                        tAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAg                                    <  2:779605/100‑1 (MQ=255)
                                                          aaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGaa                                  >  1:63294/1‑100 (MQ=255)
                                                           aaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAt                                 >  1:1502301/1‑100 (MQ=255)
                                                                aTCCGGTGAATGTGGTCGGAAAACAAAGTGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCa                            >  2:2117013/1‑100 (MQ=255)
                                                                  ccGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                          <  2:2174012/100‑1 (MQ=255)
                                                                  ccGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                          <  1:1176807/100‑1 (MQ=255)
                                                                    ggTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                        >  2:1815015/1‑100 (MQ=255)
                                                                    ggTGAATGGGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                        <  2:547914/100‑1 (MQ=255)
                                                                     gTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                       <  1:2885828/100‑1 (MQ=255)
                                                                     gTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                       <  2:38956/100‑1 (MQ=255)
                                                                          tgtgGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCGCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAc                    <  2:821299/98‑1 (MQ=255)
                                                                          tgtgGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACgg                  >  1:434378/1‑100 (MQ=255)
                                                                           gtgGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACggg                 >  1:2937988/1‑100 (MQ=255)
                                                                            tgGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGc                >  2:1366397/1‑100 (MQ=255)
                                                                             ggTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt               >  1:890990/1‑100 (MQ=255)
                                                                              gTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGTCTg              >  1:1989851/1‑100 (MQ=255)
                                                                               tCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc             >  2:1545214/1‑100 (MQ=255)
                                                                                 ggAAAACAAAGAGGAAA‑GGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa            >  2:2203112/1‑100 (MQ=255)
                                                                                 ggAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGCAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGcca           >  2:2369361/1‑98 (MQ=255)
                                                                                  gAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg          >  1:2702102/1‑100 (MQ=255)
                                                                                   aaaaCAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGt         <  1:2221862/100‑1 (MQ=255)
                                                                                    aaaCAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGtt        <  1:2871164/100‑1 (MQ=255)
                                                                                      aCAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc      <  1:1319202/100‑1 (MQ=255)
                                                                                      aCAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc      <  2:2702078/100‑1 (MQ=255)
                                                                                      aCAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc      <  2:2279385/100‑1 (MQ=255)
                                                                                      aCAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGACGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc      >  2:1618747/1‑100 (MQ=255)
                                                                                       cAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa     <  1:2215815/100‑1 (MQ=255)
                                                                                       cAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa     >  2:754534/1‑100 (MQ=255)
                                                                                        aaaGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGTCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg    >  2:1865840/1‑100 (MQ=255)
                                                                                        aaaGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg    <  2:650453/100‑1 (MQ=255)
                                                                                        aaaGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg    >  2:461225/1‑100 (MQ=255)
                                                                                        aaaGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg    <  2:2804490/100‑1 (MQ=255)
                                                                                        aaaGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAg    >  1:1043962/1‑100 (MQ=255)
                                                                                         aaGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGa   >  2:2369291/1‑100 (MQ=255)
                                                                                          agagGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc  >  2:767822/1‑100 (MQ=255)
                                                                                          agagGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAc  <  2:2775697/100‑1 (MQ=255)
                                                                                                  ||                                                                                            
AGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGAC  >  NC_000913/379915‑380106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑