Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,595,315 (A)5→4 coding (166/1278 nt) livM ← branched‑chain amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,595,3110A.100.0% 473.5 / NA 104coding (170/1278 nt)livMbranched‑chain amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base . (38/66);  total (38/66)

Only 100 of 112 total aligned reads displayed.

CTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCA  >  NC_000913/3595214‑3595410
                                                                                                 |                                                                                                   
cTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaa                                                                                                   >  2:1925166/1‑100 (MQ=255)
  gCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAACCATCGGTCCGGAAACGATTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTAGAAGaaaag                                                                                                 >  2:1522754/1‑99 (MQ=255)
  gCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGACGGTCGCAAAAGCTGGAAGaaaag                                                                                                 >  1:590591/1‑99 (MQ=255)
   cTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaaga                                                                                                <  2:1109160/100‑3 (MQ=255)
     tCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaagacc                                                                                              <  1:2619407/100‑5 (MQ=255)
       aCCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGaccac                                                                                           <  1:1225887/100‑1 (MQ=255)
         cGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGATGAAAAGACCAccg                                                                                         <  2:2253202/100‑1 (MQ=255)
          gTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCAccgc                                                                                        >  1:88979/1‑100 (MQ=255)
          gTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCAccgc                                                                                        >  1:1561884/1‑100 (MQ=255)
           tGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTAAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCAccgcc                                                                                       >  2:1922757/1‑100 (MQ=255)
            ggAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCAccgccg                                                                                      >  2:2703449/1‑100 (MQ=255)
             gAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGt                                                                                     >  2:1192317/1‑100 (MQ=255)
              aGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTg                                                                                    >  2:242165/1‑100 (MQ=255)
                ccATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGcc                                                                                  <  1:2563492/100‑1 (MQ=255)
                ccATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGcc                                                                                  <  1:2325365/100‑1 (MQ=255)
                 cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCg                                                                                 >  2:816093/1‑100 (MQ=255)
                 cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCg                                                                                 >  1:3037/1‑100 (MQ=255)
                 cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCg                                                                                 >  1:281257/1‑100 (MQ=255)
                 cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCg                                                                                 <  2:1343147/100‑1 (MQ=255)
                 cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCg                                                                                 >  2:1594558/1‑100 (MQ=255)
                  aTCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGa                                                                                <  2:2878018/100‑1 (MQ=255)
                      aTGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATaaa                                                                            <  1:999074/100‑1 (MQ=255)
                       tGGCGGGCAGAATACACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAac                                                                           <  2:2063347/100‑1 (MQ=255)
                        ggCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAaca                                                                          <  2:4675/100‑1 (MQ=255)
                         gCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAacac                                                                         <  1:1172079/100‑1 (MQ=255)
                         gCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAacac                                                                         <  1:266899/100‑1 (MQ=255)
                           gggCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACAccc                                                                       >  1:869529/1‑100 (MQ=255)
                           gggCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACAccc                                                                       <  2:598492/100‑1 (MQ=255)
                             gCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCAc                                                                     >  2:3010454/1‑100 (MQ=255)
                               aGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTg                                                                   <  1:131235/100‑1 (MQ=255)
                                gAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGc                                                                  >  1:2268110/1‑100 (MQ=255)
                                 aaTAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGcc                                                                 <  2:1711120/100‑1 (MQ=255)
                                  aTAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCa                                                                <  1:1347214/100‑1 (MQ=255)
                                   tAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCaa                                                               >  1:1163602/1‑100 (MQ=255)
                                    aaaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAAc                                                              <  1:13945/100‑1 (MQ=255)
                                     aaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACg                                                             <  2:1732377/100‑1 (MQ=255)
                                     aaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACg                                                             <  1:1773332/100‑1 (MQ=255)
                                      aCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACgg                                                            <  2:2764786/100‑1 (MQ=255)
                                       cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                           <  1:230725/100‑1 (MQ=255)
                                       cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                           <  1:81095/100‑1 (MQ=255)
                                       cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                           <  1:771419/100‑1 (MQ=255)
                                       cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                           >  1:3016380/1‑100 (MQ=255)
                                       cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                           <  2:659659/100‑1 (MQ=255)
                                           ggTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATc                                                       <  2:2226121/100‑1 (MQ=255)
                                            gTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATcc                                                      >  1:629793/1‑100 (MQ=255)
                                              ccGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGa                                                    >  1:531742/1‑100 (MQ=255)
                                                ggAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAg                                                  <  1:706356/100‑1 (MQ=255)
                                                ggAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAg                                                  <  1:3010480/100‑1 (MQ=255)
                                                 gAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGc                                                 <  1:1156643/100‑1 (MQ=255)
                                                 gAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGc                                                 <  1:1638249/100‑1 (MQ=255)
                                                 gAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGc                                                 <  1:1925184/100‑1 (MQ=255)
                                                 gAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGc                                                 <  1:2599026/100‑1 (MQ=255)
                                                  aaaCGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGcc                                                <  1:2634771/100‑1 (MQ=255)
                                                   aaCGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCg                                               >  1:2218278/1‑100 (MQ=255)
                                                     cGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGccatg                                             >  1:260891/1‑97 (MQ=255)
                                                     cGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCgtg                                             >  1:1538051/1‑100 (MQ=255)
                                                       cTTTTCAATCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc                                           <  1:1645256/100‑1 (MQ=255)
                                                       cTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc                                           >  1:1963997/1‑100 (MQ=255)
                                                       cTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc                                           >  2:2614180/1‑100 (MQ=255)
                                                        ttttCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCg                                          >  1:2391932/1‑100 (MQ=255)
                                                        atctCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCg                                          <  2:456895/97‑1 (MQ=255)
                                                         tttCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGa                                         <  2:2376519/100‑1 (MQ=255)
                                                          ttCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGac                                        >  2:403001/1‑100 (MQ=255)
                                                           tCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacc                                       <  2:1520825/100‑1 (MQ=255)
                                                            cAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGagat                                      >  1:456899/1‑97 (MQ=255)
                                                             aaCCCTTTCTCACAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCTACTGCCAACAGACATCCGAAGCCGTGTCGacca                                      <  2:1823252/99‑1 (MQ=255)
                                                              aCCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGaccacc                                    <  1:2490447/100‑1 (MQ=255)
                                                              aCCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGaccacc                                    >  1:644514/1‑100 (MQ=255)
                                                              aCCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGaccacc                                    <  1:2964489/100‑1 (MQ=255)
                                                                      tGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTgg                             <  2:127097/99‑1 (MQ=255)
                                                                       ggAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTg                           <  1:2327121/100‑1 (MQ=255)
                                                                       ggAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTg                           >  1:2157871/1‑100 (MQ=255)
                                                                       ggAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTg                           >  1:2032258/1‑100 (MQ=255)
                                                                       ggAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTg                           <  1:1434311/100‑1 (MQ=255)
                                                                        gAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGc                          <  2:531736/100‑1 (MQ=255)
                                                                        gAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGc                          <  1:2095199/100‑1 (MQ=255)
                                                                          aaGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCa                        <  2:2415378/100‑1 (MQ=255)
                                                                           aGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCAt                       <  1:1053407/100‑1 (MQ=255)
                                                                            gCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATc                      >  2:453669/1‑100 (MQ=255)
                                                                             ccGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATcc                     <  1:2703473/100‑1 (MQ=255)
                                                                             ccGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATcc                     <  2:3037/100‑1 (MQ=255)
                                                                             ccGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATcc                     <  2:2948617/100‑1 (MQ=255)
                                                                             ccGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATcc                     <  2:1569858/100‑1 (MQ=255)
                                                                             ccGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATcc                     <  2:1016670/100‑1 (MQ=255)
                                                                             ccGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATcc                     <  1:953292/100‑1 (MQ=255)
                                                                             ccGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATcc                     <  1:1496574/100‑1 (MQ=255)
                                                                              cGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCa                    <  2:1163591/100‑1 (MQ=255)
                                                                              cGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCa                    <  1:2351162/100‑1 (MQ=255)
                                                                               ggTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCCCCAGTTTGGTGCCATCCAg                   >  1:1725753/1‑100 (MQ=255)
                                                                               ggTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAg                   <  2:216854/100‑1 (MQ=255)
                                                                                ggttgaaaaGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGc                  <  2:2055005/95‑1 (MQ=255)
                                                                                   gCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc               >  1:2384035/1‑100 (MQ=255)
                                                                                   gCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc               >  2:2319682/1‑100 (MQ=255)
                                                                                   gCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc               <  2:2313589/100‑1 (MQ=255)
                                                                                    cAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCa              >  1:1619490/1‑100 (MQ=255)
                                                                                      aaaGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGt            <  2:2533705/100‑1 (MQ=255)
                                                                                         gCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGc         <  2:281254/100‑1 (MQ=255)
                                                                                          cTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCa        >  2:2444737/1‑100 (MQ=255)
                                                                                                gaaAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGccc   >  1:2107176/1‑100 (MQ=255)
                                                                                                 aaaaaGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCa  <  1:1987391/100‑1 (MQ=255)
                                                                                                 |                                                                                                   
CTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCACGCCCA  >  NC_000913/3595214‑3595410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: