BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,494,883	(A)_7→8	coding (261/564 nt)	idnK →	D‑gluconate kinase, thermosensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,494,876	1	.	A	92.6%	400.4 / 22.5	121	coding (254/564 nt)	idnK	D‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand): ref base . (5/4); new base A (57/55); total (62/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 141 total aligned reads displayed.

TATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGC  >  NC_000913/4494777‑4494974

tATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAt‑t                                                                                                          <  2:2988741/100‑1 (MQ=255)

   cTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑aa‑a                                                                                                   >  2:1896966/1‑100 (MQ=255)

    tgatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑aa‑aa                                                                                                  <  1:355971/100‑1 (MQ=255)

    tgatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑aa‑aa                                                                                                  >  1:25189/1‑100 (MQ=255)

    tgatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑aa‑aa                                                                                                  >  2:2305449/1‑100 (MQ=255)

       tgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑aa‑aaa‑a‑a                                                                                             >  1:897537/1‑100 (MQ=255)

       tgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑aa‑aaa‑a‑a                                                                                             >  2:726532/1‑100 (MQ=255)

           gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑aa‑aaa‑a‑aacag                                                                                         <  1:3006593/100‑5 (MQ=255)

           gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑aa‑aaa‑a‑aacag                                                                                         <  2:2212580/100‑5 (MQ=255)

             tCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTa                                                                                        >  1:3315/1‑100 (MQ=255)

                aCTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCg                                                                                     >  1:2267761/1‑100 (MQ=255)

                aCTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCg                                                                                     >  1:1253649/1‑100 (MQ=255)

                aCTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCg                                                                                     <  1:2141442/100‑1 (MQ=255)

                aCTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCg                                                                                     <  2:1835921/100‑1 (MQ=255)

                 cTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGt                                                                                    <  2:2479777/100‑1 (MQ=255)

                  ttCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTg                                                                                   <  2:496125/100‑1 (MQ=255)

                   tCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGa                                                                                  <  1:2907946/100‑1 (MQ=255)

                    cccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGat                                                                                 >  2:1399016/1‑100 (MQ=255)

                       tggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATAtt                                                                              <  2:1970328/100‑1 (MQ=255)

                        ggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATAttt                                                                             <  2:1021441/100‑1 (MQ=255)

                        ggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATAttt                                                                             <  2:1307594/100‑1 (MQ=255)

                        ggctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATAttt                                                                             >  2:1442297/1‑100 (MQ=255)

                           tggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTa                                                                           <  2:1216673/99‑1 (MQ=255)

                           tggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTAc                                                                          <  1:1379946/100‑1 (MQ=255)

                            ggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACg                                                                         <  1:2825945/100‑1 (MQ=255)

                                cGCTTAAATGATGGTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAg                                                                     >  2:1877587/1‑100 (MQ=255)

                                cGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAg                                                                     <  1:1352725/100‑1 (MQ=255)

                                 gCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAgg                                                                    >  1:2900262/1‑100 (MQ=255)

                                  cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAggg                                                                   <  1:30410/100‑1 (MQ=255)

                                  cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAggg                                                                   >  2:2545434/1‑100 (MQ=255)

                                  cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAggg                                                                   >  2:1056297/1‑100 (MQ=255)

                                  cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAg                                                                     >  2:2842446/1‑98 (MQ=255)

                                  cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGt                                                                  >  1:1539126/1‑100 (MQ=255)

                                   ttAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGTATTTATTGTCTGTTAATCAT‑T‑‑‑AA‑AAA‑ACACAGTATCCTGATAtttt                                                                            >  2:2733594/1‑90 (MQ=255)

                                    tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCATCA‑‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTa                                                                 >  1:97222/1‑100 (MQ=255)

                                    tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAg                                                                     >  2:1085217/1‑96 (MQ=255)

                                    tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTa                                                                 <  2:2221276/100‑1 (MQ=255)

                                    tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAg                                                                >  2:111162/1‑100 (MQ=255)

                                    tAAATGATCGTTCAGACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTa                                                                 <  2:1796250/100‑1 (MQ=255)

                                     aaaTGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAg                                                                <  1:2968494/100‑1 (MQ=255)

                                         gatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGcccc                                                            <  1:1000845/100‑1 (MQ=255)

                                         gatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGccc                                                             >  1:123137/1‑99 (MQ=255)

                                         gatgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGc                                                               >  2:3002645/1‑97 (MQ=255)

                                           tgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCa                                                          <  1:1442309/100‑1 (MQ=255)

                                           tgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCa                                                          <  2:2663176/100‑1 (MQ=255)

                                           tgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCAt                                                         <  2:1539113/100‑1 (MQ=255)

                                           tgCTTCATACAGTCCTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCa                                                          <  1:794125/100‑1 (MQ=255)

                                            gCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCAt                                                         >  2:869840/1‑100 (MQ=255)

                                             cTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATg                                                        >  1:294766/1‑100 (MQ=255)

                                               tCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGtt                                                      <  1:2301003/100‑1 (MQ=255)

                                                  tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAt                                                   >  1:2091566/1‑100 (MQ=255)

                                                   aCAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAtt                                                  >  1:2544121/1‑100 (MQ=255)

                                                   aCAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAtt                                                  >  2:2959092/1‑100 (MQ=255)

                                                   aCAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAtt                                                  >  2:610413/1‑100 (MQ=255)

                                                      gTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTcc                                               <  1:2695113/100‑1 (MQ=255)

                                                      gTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTc                                                >  2:724145/1‑99 (MQ=255)

                                                         tttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑AACAAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTc                                                >  2:961163/1‑96 (MQ=255)

                                                          ttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTg                                           <  2:1424832/100‑1 (MQ=255)

                                                           tataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTgg                                          <  2:1949713/100‑1 (MQ=255)

                                                           tataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTgg                                          >  2:196582/1‑100 (MQ=255)

                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                        <  2:867458/100‑1 (MQ=255)

                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                        <  1:1071444/100‑1 (MQ=255)

                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                        <  2:944638/100‑1 (MQ=255)

                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                        <  1:2115459/100‑1 (MQ=255)

                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                        >  2:1071434/1‑100 (MQ=255)

                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                        <  1:2017140/100‑1 (MQ=255)

                                                             taAAAAGAATGAAACAGGATATATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                        >  2:1858164/1‑100 (MQ=255)

                                                             taAAAAGAAGGAAACAGGATTTATTGTCTGTTCATAAA‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                        <  2:683790/100‑1 (MQ=255)

                                                              aaaaaGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                        >  1:868317/1‑99 (MQ=255)

                                                              aaaaaGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTa                                       >  2:2208261/1‑100 (MQ=255)

                                                              aaaaaGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTa                                       <  1:724152/100‑1 (MQ=255)

                                                              aaaaaGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTa                                       <  2:1385477/100‑1 (MQ=255)

                                                              aaaaaGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTa                                       >  2:511370/1‑100 (MQ=255)

                                                                  aGAATGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATg                                   <  2:451245/100‑1 (MQ=255)

                                                                   gAATGAAACAGGATTTATTGTCTGTTCATCAT‑TAA‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGTGTAGCCCCCATGTTCATTTCCTCTGGTTAGATg                                   <  1:671806/100‑1 (MQ=255)

                                                                     aTGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCg                                <  1:1834512/100‑1 (MQ=255)

                                                                     aTGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCg                                <  1:151557/100‑1 (MQ=255)

                                                                      tGAAACAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGa                               <  1:883771/100‑1 (MQ=255)

                                                                        aaaCAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACt                             >  1:3012421/1‑100 (MQ=255)

                                                                        aaaCAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACt                             <  1:44033/100‑1 (MQ=255)

                                                                        aaaCAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCGGGTTAGATGGCGACt                             >  1:1039731/1‑100 (MQ=255)

                                                                          aCAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTAt                           <  2:2091548/100‑1 (MQ=255)

                                                                          aCAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTAt                           <  2:3315/100‑1 (MQ=255)

                                                                          aCAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTAt                           <  1:560768/100‑1 (MQ=255)

                                                                           cAGGATTTATTGTCTGTTCATCAT‑TAAAAA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGa                               >  2:2188519/1‑97 (MQ=255)

                                                                           cAGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATg                          <  1:1399028/100‑1 (MQ=255)

                                                                            aGGATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGa                         <  1:1708259/100‑1 (MQ=255)

                                                                            aGGATTTATTGTCTGTTCATCAT‑T‑‑‑AA‑AAACA‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGa                         <  2:2450121/100‑1 (MQ=255)

                                                                             ggATTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGaa                        >  2:2461017/1‑100 (MQ=255)

                                                                               aattATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                      >  1:2043910/3‑100 (MQ=255)

                                                                               aTTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                      >  2:1813459/1‑100 (MQ=255)

                                                                               aTTTATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAAc                      <  2:2267741/100‑1 (MQ=255)

                                                                                  tATTGTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTAt                   >  1:4613/1‑100 (MQ=255)

                                                                                      gTCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtctc               >  2:1857245/1‑100 (MQ=255)

                                                                                       tCTGTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcg              >  2:1126146/1‑100 (MQ=255)

                                                                                          gTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc           >  2:418165/1‑100 (MQ=255)

                                                                                          gTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc           >  1:332010/1‑100 (MQ=255)

                                                                                          gTTCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc           >  2:2498979/1‑100 (MQ=255)

                                                                                           ttCATCAT‑TA‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgcg          <  2:1335187/100‑1 (MQ=255)

                                                                                                  t‑t‑‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGc  >  1:2697838/1‑100 (MQ=255)

TATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCAT‑T‑‑‑AA‑AAA‑A‑ACAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATGCAGC  >  NC_000913/4494777‑4494974

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`