BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,638,193	(C)_6→7	coding (16/1353 nt)	creD →	inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,638,187	1	.	C	98.0%	353.7 / ‑0.1	101	coding (10/1353 nt)	creD	inner membrane protein
Reads supporting (aligned to +/- strand): ref base . (1/1); new base C (47/51); total (49/52)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.60e-01

Only 100 of 116 total aligned reads displayed.

GCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑CCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGAT  >  NC_000913/4638088‑4638285

gCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAt                                                                                                     >  2:1552268/1‑100 (MQ=255)

  cTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑cc                                                                                                  >  2:663094/1‑100 (MQ=255)

  cTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑cc                                                                                                  <  1:31438/100‑1 (MQ=255)

   tCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑ccc                                                                                                 >  2:947201/1‑100 (MQ=255)

    cGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑cccc                                                                                                >  2:1334344/1‑100 (MQ=255)

    cGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑cccc                                                                                                >  1:1635103/1‑100 (MQ=255)

     gCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑ccccc                                                                                               <  2:426036/100‑1 (MQ=255)

     gCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑ccccc                                                                                               <  2:1671218/100‑1 (MQ=255)

      cTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑cccccc                                                                                              >  2:2750022/1‑100 (MQ=255)

      cTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑cccccc                                                                                              <  1:523442/100‑1 (MQ=255)

      cTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑cccccc                                                                                              <  1:2912284/100‑1 (MQ=255)

      cTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑ccccc                                                                                               >  1:2585390/1‑99 (MQ=255)

       ttCGACTTCACCGTCACTTCACATAGCTTCAACTTCTCCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑ccccccc                                                                                             <  2:1266305/100‑2 (MQ=255)

       ttCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑ccccccc                                                                                             <  1:2148939/100‑2 (MQ=255)

            cTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGTCTATGTTGAAATCCCCCCCTGTTc                                                                                         >  1:1143484/1‑100 (MQ=255)

            cTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTc                                                                                         >  2:535795/1‑100 (MQ=255)

             ttCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCt                                                                                        <  2:2563490/100‑1 (MQ=255)

              tCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTg                                                                                       <  1:985068/100‑1 (MQ=255)

              tCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTg                                                                                       <  2:790819/100‑1 (MQ=255)

               cACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTgg                                                                                      <  1:2322626/100‑1 (MQ=255)

               cACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTgg                                                                                      >  2:930387/1‑100 (MQ=255)

                aCCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGa                                                                                     <  1:1408036/100‑1 (MQ=255)

                 ccGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGaa                                                                                    <  2:2926708/100‑1 (MQ=255)

                 ccGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGaa                                                                                    <  1:2013249/100‑1 (MQ=255)

                  cGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGaaa                                                                                   >  1:2333217/1‑100 (MQ=255)

                   gTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGaaaa                                                                                  <  1:930395/100‑1 (MQ=255)

                   gTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGaaaa                                                                                  >  1:1211542/1‑100 (MQ=255)

                    tCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAAt                                                                                 <  1:93277/100‑1 (MQ=255)

                     cACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATg                                                                                >  2:2600786/1‑100 (MQ=255)

                     cACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATg                                                                                <  1:2302671/100‑1 (MQ=255)

                      aCTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGa                                                                               >  1:2542379/1‑100 (MQ=255)

                       cTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGAc                                                                              <  1:2782426/100‑1 (MQ=255)

                         tCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTa                                                                            >  1:1607897/1‑100 (MQ=255)

                         tCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTa                                                                            <  2:1642685/100‑1 (MQ=255)

                           acaTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGc                                                                          <  2:392367/100‑1 (MQ=255)

                            caTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGcc                                                                         >  2:31174/1‑100 (MQ=255)

                            caTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGcc                                                                         <  1:294319/100‑1 (MQ=255)

                            caTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGcc                                                                         >  2:274066/1‑100 (MQ=255)

                            caTAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGG‑AAATGACTAGCCt                                                                        >  2:2631333/1‑100 (MQ=255)

                              tAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTg                                                                       >  2:1959405/1‑100 (MQ=255)

                                gCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGtt                                                                     <  2:419894/100‑1 (MQ=255)

                                gCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGtt                                                                     <  1:2771258/100‑1 (MQ=255)

                                gCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGtt                                                                     >  1:1089076/1‑100 (MQ=255)

                                gCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGtt                                                                     >  2:2453098/1‑100 (MQ=255)

                                gCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGtt                                                                     >  2:597005/1‑100 (MQ=255)

                                   tCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTgg                                                                  <  1:1434666/100‑1 (MQ=255)

                                      aaTTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGc                                                               <  2:2975218/100‑1 (MQ=255)

                                        ttcttcCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAg                                                             <  2:2167268/100‑1 (MQ=255)

                                        ttcttcCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAg                                                             >  1:1323/1‑100 (MQ=255)

                                         tcttcCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGt                                                            <  2:2333197/100‑1 (MQ=255)

                                            tcCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTAtt                                                         >  1:885224/1‑100 (MQ=255)

                                               cacaTAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCt                                                      <  2:2244979/100‑1 (MQ=255)

                                                acaTAGTCTTCGTATCCTGCTCCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCtg                                                     <  2:2236402/100‑1 (MQ=255)

                                                 caTAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCtgt                                                    <  1:947209/100‑1 (MQ=255)

                                                   tAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGATGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATCGCtgttg                                                  <  2:1596352/100‑1 (MQ=255)

                                                   tAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCtgttg                                                  <  2:1607883/100‑1 (MQ=255)

                                                      tCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGttgttg                                               <  2:1324745/100‑1 (MQ=255)

                                                       cTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGa                                              >  2:788233/1‑100 (MQ=255)

                                                       cTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGa                                              <  2:2094283/100‑1 (MQ=255)

                                                          cGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTc                                           <  1:1905609/100‑1 (MQ=255)

                                                              tCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGAt                                       <  2:1211531/100‑1 (MQ=255)

                                                               ccTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATa                                      >  1:551299/1‑100 (MQ=255)

                                                                  gctgcCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATg                                   >  2:1895849/1‑100 (MQ=255)

                                                                  gctgcCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATg                                   <  1:2750046/100‑1 (MQ=255)

                                                                    tgcCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCt                                 >  2:903187/1‑100 (MQ=255)

                                                                    tgcCATTGCAAAGGAGAAGAATATGTTGAAATACCCCCCTGTGCTGGAAAATGACTAGCCTGATTGCTGCAGTACTGCTGTTGTTGATTCCGATAATGCt                                 >  2:2505146/1‑100 (MQ=255)

                                                                     gcCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTg                                >  1:58011/1‑100 (MQ=255)

                                                                     gcCATTGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTg                                <  1:1552281/100‑1 (MQ=255)

                                                                         ttGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTc                            <  1:1862330/100‑1 (MQ=255)

                                                                         ttGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTc                            <  1:1860328/100‑1 (MQ=255)

                                                                          tGCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCg                           >  2:361335/1‑100 (MQ=255)

                                                                           gCAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCgg                          >  1:427759/1‑100 (MQ=255)

                                                                            cAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGc                         >  1:263687/1‑100 (MQ=255)

                                                                            cAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGc                         >  1:2386290/1‑100 (MQ=255)

                                                                            cAAAGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGc                         >  2:478322/1‑100 (MQ=255)

                                                                              aaGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAg                       >  2:2139200/1‑100 (MQ=255)

                                                                               aGGAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAgg                      <  1:2768316/100‑1 (MQ=255)

                                                                                ggAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGt                     >  2:2141993/1‑100 (MQ=255)

                                                                                ggAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGt                     >  1:1206604/1‑100 (MQ=255)

                                                                                ggAGAAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGt                     <  1:2551207/100‑1 (MQ=255)

                                                                                 gagaAGACTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTg                    >  2:1385332/1‑100 (MQ=255)

                                                                                  agaagaCTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGa                   >  1:2340176/1‑100 (MQ=255)

                                                                                   gaagaCTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGAt                  >  1:2377983/1‑100 (MQ=255)

                                                                                     agaCTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTg                >  1:1939298/1‑100 (MQ=255)

                                                                                      gaCTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGt               <  2:1635089/100‑1 (MQ=255)

                                                                                       aCTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGATTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTc              <  2:1939280/100‑1 (MQ=255)

                                                                                        cTATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTc              <  2:1143474/99‑1 (MQ=255)

                                                                                         tATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGa            <  1:1343312/100‑1 (MQ=255)

                                                                                         tATGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGa            >  2:1497606/1‑100 (MQ=255)

                                                                                          aTGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGaa           >  1:2623577/1‑100 (MQ=255)

                                                                                          aTGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGCCAGGTGATTGTCGaa           >  1:2983468/1‑100 (MQ=255)

                                                                                           tGTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAAc          >  2:1016266/1‑100 (MQ=255)

                                                                                            gTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACg         >  2:2238799/1‑100 (MQ=255)

                                                                                            gTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACg         <  2:2218239/100‑1 (MQ=255)

                                                                                            gTTGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACg         <  1:2706217/100‑1 (MQ=255)

                                                                                             ttGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGt        >  1:1303733/1‑100 (MQ=255)

                                                                                             ttGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCAGCAGGTGATTGTCGAACGt        >  1:1607346/1‑100 (MQ=255)

                                                                                              tGAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTAGAACGTg       >  2:2623862/1‑100 (MQ=255)

                                                                                               gAAATCCCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGc      <  2:1402469/100‑1 (MQ=255)

                                                                                                  aT‑CCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGAt  <  2:2216667/100‑1 (MQ=255)

GCCTCGCTTCGACTTCACCGTCACTTCACATAGCTTCAAATTCTTCCCACATAGTCTTCGTATCCTGCTGCCATTGCAAAGGAGAAGACTATGTTGAAAT‑CCCCCCTGTTCTGGAAAATGACTAGCCTGTTTGGTGCAGTATTGCTGTTGTTGATTCCGATAATGCTGATTCGGCAGGTGATTGTCGAACGTGCTGAT  >  NC_000913/4638088‑4638285

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`