Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 233,773 +A coding (183/1359 nt) mltD ← putative membrane‑bound lytic murein transglycosylase D

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913233,7701.A99.1% 364.1 / ‑3.6 106coding (186/1359 nt)mltDputative membrane‑bound lytic murein transglycosylase D
Reads supporting (aligned to +/- strand):  ref base . (0/1);  new base A (46/59);  total (46/60)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.91e-01

Only 100 of 118 total aligned reads displayed.

AGTTACATCGTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTG  >  NC_000913/233671‑233864
                                                                                                    |                                                                                              
aGTTACATCGTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATg                                                                                                 <  1:295697/100‑1 (MQ=255)
aGTTACATCGTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATg                                                                                                 >  2:2154078/1‑100 (MQ=255)
 gTTACATCGTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGa                                                                                               <  2:2969918/100‑1 (MQ=255)
  ttACATCGTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGaa                                                                                              >  2:1405930/1‑100 (MQ=255)
   tACATCGTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGaaa                                                                                             <  1:2435079/100‑1 (MQ=255)
    aCATCGTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGaaaa                                                                                            >  2:366033/1‑99 (MQ=255)
      agcGTGGAGATAGGTCTTATTGCGTAAATATTTCTGTTTCTGATCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGaaaagc                                                                                          >  1:2051478/3‑97 (MQ=255)
        cGTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGaaaagccc                                                                                        <  2:2604198/100‑6 (MQ=255)
        cGTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGaaaagccc                                                                                        <  2:2658633/100‑6 (MQ=255)
        cGTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGaaaagccc                                                                                        <  1:2898203/100‑6 (MQ=255)
         gTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCca                                                                                        <  2:137734/100‑1 (MQ=255)
         gTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCca                                                                                        <  2:2481199/100‑1 (MQ=255)
         gTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCca                                                                                        <  2:543395/100‑1 (MQ=255)
          tGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCcac                                                                                       <  1:1709785/100‑1 (MQ=255)
           ggAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCcaca                                                                                      <  2:2718054/100‑1 (MQ=255)
            gagaTAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACaa                                                                                     >  1:2725989/1‑100 (MQ=255)
             agaTAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAg                                                                                    <  2:525619/100‑1 (MQ=255)
             agaTAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAg                                                                                    <  1:1149619/100‑1 (MQ=255)
             agaTAGCTCTTATTGCGTAAATATTTCTGTTTCGATTCGCGAATCCGGTCATTTTCCGGAATGCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAg                                                                                    <  2:2317893/100‑1 (MQ=255)
              gaTAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGTCCACaa                                                                                     >  1:704443/1‑98 (MQ=255)
                 aGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCAc                                                                                >  2:2182235/1‑100 (MQ=255)
                  gCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCAcc                                                                               >  2:1807810/1‑100 (MQ=255)
                     ctTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCt                                                                           >  2:781795/1‑100 (MQ=255)
                        aTTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTgg                                                                         <  1:2854146/100‑1 (MQ=255)
                        aTTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTgg                                                                         <  2:1554140/100‑1 (MQ=255)
                        aTTGCGTAAATATGTCTGTTTCTGTTCGCGAGTCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTgg                                                                         >  1:2038830/1‑100 (MQ=255)
                           gCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGgcgc                                                                      <  1:381095/100‑1 (MQ=255)
                            cGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGcgcg                                                                     <  2:745993/100‑1 (MQ=255)
                              tAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGAt                                                                   >  1:312065/1‑100 (MQ=255)
                              tAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGAt                                                                   >  2:1460535/1‑100 (MQ=255)
                              tAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCATCGCCACTGAAAAGCCCACAAGTCACCATCTGGCGCGAt                                                                   >  2:2124913/1‑100 (MQ=255)
                                aaTATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAg                                                                 <  1:184071/100‑1 (MQ=255)
                                  tatTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGTAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGAc                                                               >  2:2884423/1‑100 (MQ=255)
                                   atTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACg                                                              >  1:2914061/1‑100 (MQ=255)
                                    tttctgtttctgTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGt                                                             >  2:1052758/1‑100 (MQ=255)
                                      tctgtttctgttCGCGACTTCGGTTATTTTCCGGACTTAACATCTTTCCATCGTCGCCAATGCCAGCCCCCCTGTCACCCTCTGGCGCGATCGACGTccc                                                          <  1:2883791/100‑1 (MQ=255)
                                      tctgtttctgttCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCTCCAATGAAAAGCCCACAAGGCACCATCTGGCGCGATAGACGTcc                                                           >  2:250042/1‑100 (MQ=255)
                                       ctgtttctgttCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTccc                                                          <  2:2112932/100‑1 (MQ=255)
                                           ttctgttCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCg                                                      >  2:2403246/1‑100 (MQ=255)
                                            tctgttCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGt                                                     <  1:1546412/100‑1 (MQ=255)
                                             ctgttCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGtc                                                    >  2:1740404/1‑100 (MQ=255)
                                             ctgttCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGtc                                                    >  1:2230019/1‑100 (MQ=255)
                                              tgttCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGtcc                                                   <  2:2513079/100‑1 (MQ=255)
                                               gttCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGtcca                                                  <  2:2051460/100‑1 (MQ=255)
                                               gttCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGtcca                                                  >  1:2873480/1‑100 (MQ=255)
                                               gttCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGtcca                                                  >  2:1155174/1‑100 (MQ=255)
                                                ttCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGtccat                                                 <  2:312062/100‑1 (MQ=255)
                                                ntCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGtccat                                                 >  1:1414893/2‑100 (MQ=255)
                                                  cgcgAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGtccatcc                                               >  1:1571937/1‑100 (MQ=255)
                                                  cgcgAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGtccatcc                                               >  1:1230068/1‑100 (MQ=255)
                                                  cgcgAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGtccatcc                                               >  2:1057251/1‑100 (MQ=255)
                                                     gAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCcatccatc                                            <  1:2391248/100‑1 (MQ=255)
                                                      aaTCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCg                                           <  1:1664454/100‑1 (MQ=255)
                                                      aaTCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCg                                           >  1:972768/1‑100 (MQ=255)
                                                          cGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGcc                                       >  1:1104770/1‑100 (MQ=255)
                                                          cGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGcc                                       >  1:1104287/1‑100 (MQ=255)
                                                          cGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGcc                                       <  1:246284/100‑1 (MQ=255)
                                                          cGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGcc                                       >  1:2406802/1‑100 (MQ=255)
                                                          cGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGcc                                       <  1:2273021/100‑1 (MQ=255)
                                                          cGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGcc                                       >  1:441219/1‑100 (MQ=255)
                                                          cGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGcc                                       >  1:1960828/1‑100 (MQ=255)
                                                          cGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGcc                                       <  1:452339/100‑1 (MQ=255)
                                                          cGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGcc                                       <  1:843147/100‑1 (MQ=255)
                                                          cGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCAAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGcc                                       <  2:2858046/100‑1 (MQ=255)
                                                           ggTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCt                                      >  2:1394679/1‑100 (MQ=255)
                                                           ggTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCt                                      >  1:2420591/1‑100 (MQ=255)
                                                            gTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTg                                     <  1:733442/100‑1 (MQ=255)
                                                              naTTTTCCGGAATTCCCATCTTTAGCTCATCGCCAATGAAAAGCCCACAAGTCACCGTCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGAc                                   >  2:1935040/2‑100 (MQ=255)
                                                               aTTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCGCAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACt                                  <  1:1895863/100‑1 (MQ=255)
                                                               aTTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACt                                  <  2:2996312/100‑1 (MQ=255)
                                                               aTTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGCCCCATCGTCCATCCATCGTGCCTGACt                                  <  1:1231109/100‑1 (MQ=255)
                                                                ttttCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACtt                                 <  2:1922848/100‑1 (MQ=255)
                                                                 tttCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTg                                >  1:2604243/1‑100 (MQ=255)
                                                                    ccGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTaa                             <  1:1155185/100‑1 (MQ=255)
                                                                     cGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTaaa                            <  2:2645390/100‑1 (MQ=255)
                                                                     cGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTaaa                            >  2:11365/1‑100 (MQ=255)
                                                                      ggAATTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAAc                           <  2:540623/100‑1 (MQ=255)
                                                                        aaTTCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACtt                         >  2:340065/1‑100 (MQ=255)
                                                                           tCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTtgn                      <  2:786200/100‑2 (MQ=255)
                                                                           tCCCATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTtgc                      <  2:788981/100‑1 (MQ=255)
                                                                            cccATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTtgct                     <  1:375285/100‑1 (MQ=255)
                                                                            cccATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTtgct                     <  2:2725965/100‑1 (MQ=255)
                                                                             ccATCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTtgctg                    <  1:1949549/100‑1 (MQ=255)
                                                                               aTCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTgctgct                  <  1:2943302/100‑1 (MQ=255)
                                                                               aTCTTTAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTgctgct                  <  1:1674407/100‑1 (MQ=255)
                                                                                  tttAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTcc               >  1:2588224/1‑100 (MQ=255)
                                                                                    tAGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTcccc             >  2:2068082/1‑100 (MQ=255)
                                                                                     aGCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCt            <  2:1194119/100‑1 (MQ=255)
                                                                                      gCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGGGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCtt           >  2:1864570/1‑100 (MQ=255)
                                                                                      gCTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCt            <  1:1895072/99‑1 (MQ=255)
                                                                                       cTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTg          <  1:2124932/100‑1 (MQ=255)
                                                                                       cTCGTCGCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTg          <  2:2588201/100‑1 (MQ=255)
                                                                                        tcgtcgCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGa         <  2:626707/100‑1 (MQ=255)
                                                                                          gtcgCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGAcc       <  2:2914035/100‑1 (MQ=255)
                                                                                             gCCAATGAAAAGCCCAGAAGTCACTATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGc    <  1:25712/100‑1 (MQ=255)
                                                                                             gCCAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGc    >  1:654076/1‑100 (MQ=255)
                                                                                             ctcAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGc    <  2:1922568/98‑1 (MQ=255)
                                                                                              ccAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCt   >  1:594246/1‑100 (MQ=255)
                                                                                               cAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGcag  >  1:1922586/1‑98 (MQ=255)
                                                                                               cAATGAAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTACACTTGACCAGCTg  >  1:557547/1‑100 (MQ=255)
                                                                                                    |                                                                                              
AGTTACATCGTGGAGATAGCTCTTATTGCGTAAATATTTCTGTTTCTGTTCGCGAATCCGGTCATTTTCCGGAATTCCCATCTTTAGCTCGTCGCCAATGAAAGCCCACAAGTCACCATCTGGCGCGATAGACGTCCCATCGTCCATCCATCGTGCCTGACTTGTAAACTTTGCTGCTTCCCCTTGACCAGCTG  >  NC_000913/233671‑233864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: