BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,224,239	0	A	T	22.1%	235.1 / 66.6	114	intergenic (+332/+40)	ycgI/minE	pseudogene/cell division topological specificity factor
Reads supporting (aligned to +/- strand): ref base A (43/45); new base T (8/17); total (51/63)
Fisher's exact test for biased strand distribution p-value = 1.73e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.27e-01

Only 100 of 118 total aligned reads displayed.

TGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATC  >  NC_000913/1224140‑1224335

tgtAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAgaga                                                                                                  >  1:2422666/1‑100 (MQ=255)

 gtAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAgaga                                                                                                  >  2:2862216/1‑99 (MQ=255)

 gtAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGAt                                                                                                 >  1:761793/1‑100 (MQ=255)

 gtAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGAt                                                                                                 <  1:2673632/100‑1 (MQ=255)

 gtAAAGGAGGAGGTGGAAATAGCAATGAAGAGTATCAGCAAGAATAATCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGAt                                                                                                 <  2:1386578/100‑1 (MQ=255)

  tAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATa                                                                                                <  2:1191060/100‑1 (MQ=255)

  tAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATa                                                                                                >  2:2948906/1‑100 (MQ=255)

    aaGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAAc                                                                                              <  2:1185131/100‑1 (MQ=255)

         ggAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGc                                                                                         >  1:2470358/1‑100 (MQ=255)

          gAGGTGGAAATAGCAATGAGGAGTATCAGCAAGATTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGATATACCTCTGcc                                                                                        <  2:1598574/100‑1 (MQ=255)

          gAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGcc                                                                                        <  1:1896665/100‑1 (MQ=255)

           aGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCt                                                                                       <  1:1422491/100‑1 (MQ=255)

             gttgAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAATCTCAAGGCAGAGTTATCTCTGCCTTg                                                                                     <  2:2836195/97‑1 (MQ=255)

             gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTTTGCCTTg                                                                                     <  1:1601439/100‑1 (MQ=255)

             gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg                                                                                     <  1:2289583/100‑1 (MQ=255)

             gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg                                                                                     <  1:1323303/100‑1 (MQ=255)

             gTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTg                                                                                     <  2:2131185/100‑1 (MQ=255)

               ggAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGag                                                                                   <  2:2647989/100‑2 (MQ=255)

                gAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagt                                                                                  <  1:293538/100‑3 (MQ=255)

                gAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagt                                                                                  <  1:1894580/100‑3 (MQ=255)

                 aaaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagtt                                                                                 <  1:2861925/100‑4 (MQ=39)

                 aaaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagtt                                                                                 <  2:2747218/100‑4 (MQ=39)

                 aaaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGa                                                                                 >  1:2091880/1‑100 (MQ=255)

                  aaTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagttt                                                                                <  2:2321120/100‑5 (MQ=38)

                   aTAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATa                                                                               <  2:47237/100‑1 (MQ=255)

                    tAGTAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagttttt                                                                              <  2:601469/100‑7 (MQ=37)

                     tgCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATaaa                                                                             <  2:2489430/99‑1 (MQ=255)

                     aGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAATCTCAAGGCAGAGATAACTCTGCCTTGAAGATaaa                                                                             <  1:2730111/100‑1 (MQ=255)

                     aGCAATGAGGAGTATCAGCAAGAATACTCGCCACTTGACCACAACGTTGTTGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCGTTGagtttttc                                                                             <  2:2953820/100‑8 (MQ=25)

                      gCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATaaaa                                                                            >  1:2489452/1‑99 (MQ=255)

                      gCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAAt                                                                            >  2:535507/1‑100 (MQ=255)

                       cAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATg                                                                           <  1:741769/100‑1 (MQ=255)

                        aaTGAGGAGTATCAGCAAGAATACTCGCCTCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGTTATCTCTGCCTTGagtttttcatc                                                                          <  2:2692633/100‑11 (MQ=25)

                        aaTGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATgc                                                                          <  2:728190/100‑1 (MQ=255)

                           gaggagTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCt                                                                       <  1:340583/100‑1 (MQ=255)

                             ggagTATCAGCAAGAATACTCACCGCTTTAGCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCttt                                                                     <  2:437130/100‑1 (MQ=255)

                                  aTCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAg                                                                >  1:2013817/1‑100 (MQ=255)

                                    cAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg                                                              >  2:1774375/1‑100 (MQ=255)

                                    cAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg                                                              <  1:2096068/100‑1 (MQ=255)

                                    cAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg                                                              >  1:2206278/1‑100 (MQ=255)

                                    cAGCAAGAATACTCGCCGCTTTACCACAACGTGGACGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCg                                                              >  2:2682538/1‑100 (MQ=255)

                                     aGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCgg                                                             <  2:2065851/100‑1 (MQ=255)

                                      gCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCggg                                                            >  1:1729163/1‑100 (MQ=255)

                                      gCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCggg                                                            <  1:1898207/100‑1 (MQ=255)

                                       ccaGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGc                                                           >  2:2840613/3‑100 (MQ=255)

                                       cAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGc                                                           >  1:1548950/1‑100 (MQ=255)

                                        aaGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCt                                                          <  1:2632567/100‑1 (MQ=255)

                                         aGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCtt                                                         >  1:1407626/1‑100 (MQ=255)

                                          gAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTa                                                        <  1:2622362/100‑1 (MQ=255)

                                            aTACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTAtt                                                      >  2:2400096/1‑100 (MQ=255)

                                              aCTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc                                                    <  1:954199/100‑1 (MQ=255)

                                              aCTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc                                                    >  1:1766493/1‑100 (MQ=255)

                                              aCTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTc                                                    <  1:2661894/100‑1 (MQ=255)

                                               cTCGCCGCTTTACCACAACGTGGATGAGAGCTATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTCCAGCGGGCTTATTTCa                                                   <  2:561173/100‑1 (MQ=255)

                                                tCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAg                                                  <  1:3028845/100‑1 (MQ=255)

                                                tCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAg                                                  <  1:783679/100‑1 (MQ=255)

                                                tCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAg                                                  >  2:3033713/1‑100 (MQ=255)

                                                 cgccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGc                                                 >  1:303695/1‑100 (MQ=255)

                                                 cgcagcTTTACCCCAACGTGGATGAGAGGGATGAAAACCTCAAGGCAGAGATAACTCTGCCTTGAAGATCAATGCGCTTTTACAGCGGGCTTATTTCAGc                                                 <  1:1364512/100‑1 (MQ=255)

                                                   ccgcTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGctc                                               >  1:1931474/1‑100 (MQ=255)

                                                   ccgcGTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGc                                                 <  2:1840357/98‑1 (MQ=255)

                                                        ttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTg                                          <  2:1719247/100‑1 (MQ=255)

                                                        ttACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTg                                          <  1:228661/100‑1 (MQ=255)

                                                         tACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGc                                         <  2:1145432/100‑1 (MQ=255)

                                                          aCCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCt                                        <  2:1122192/100‑1 (MQ=255)

                                                           ccACAACGTGGATGAGCGGGCTGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCATCTCTTATGCtt                                       >  1:1104722/1‑100 (MQ=255)

                                                             cgatcttGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTcc                                     <  2:2431307/94‑1 (MQ=255)

                                                             acaACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTcc                                     <  1:48436/100‑1 (MQ=255)

                                                               aaCGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCgg                                   >  2:24862/1‑100 (MQ=255)

                                                               aaCGTGGATGAGAGGCATGAAAAACTCAAGCCAGAGCTAACTCTGCCTTGAAGATTTATTCGCTTTTACAGCTGCCCTATTTCAGCTCTTCTGCTTct                                     <  2:427845/98‑2 (MQ=255)

                                                               aaCGTGGATGAGAGGAATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCgg                                   <  1:2990982/100‑1 (MQ=255)

                                                                 cGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCGTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTa                                 >  1:1203569/1‑100 (MQ=255)

                                                                   tGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAg                               <  1:1293438/100‑1 (MQ=255)

                                                                    ggATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAgg                              >  2:1905192/1‑100 (MQ=255)

                                                                      aTGAGAGGAATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTc                            <  1:447449/100‑1 (MQ=255)

                                                                       tGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCa                           <  1:2799963/100‑1 (MQ=255)

                                                                         agagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACg                         <  2:325769/100‑1 (MQ=255)

                                                                         agagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACg                         <  1:2862242/100‑1 (MQ=255)

                                                                          gagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGt                        <  2:2215118/100‑1 (MQ=255)

                                                                          gagGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGt                        >  1:403827/1‑100 (MQ=255)

                                                                                 naaaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTc                 >  2:330259/2‑100 (MQ=255)

                                                                                 gAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTc                 >  1:2103527/1‑100 (MQ=255)

                                                                                   aaaaCTCAAGGCAGAGATAACTCTGCCTTGAATATAAATGCGCTTTTACAGAGGGCTTATTTAAGCTGTTATGGTTCCGGTAAGATCAAGTTCAGCtaa                >  2:2663064/1‑97 (MQ=255)

                                                                                   aaaaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCaa               >  2:230730/1‑100 (MQ=255)

                                                                                     tcttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGa             >  2:2708696/4‑100 (MQ=255)

                                                                                     aaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGa             <  1:1253121/100‑1 (MQ=255)

                                                                                     aaCTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGa             >  1:2017794/1‑100 (MQ=255)

                                                                                       ttCAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAAt           >  1:901463/2‑100 (MQ=255)

                                                                                       cTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAAt           <  2:2206259/100‑1 (MQ=255)

                                                                                         cAAGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAg         >  2:495696/1‑100 (MQ=255)

                                                                                         cAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAg         >  2:2346522/1‑100 (MQ=255)

                                                                                          aaGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa        >  2:1816639/1‑100 (MQ=255)

                                                                                          aaGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGa        >  1:1911261/1‑100 (MQ=255)

                                                                                           aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaa       >  1:2616865/1‑100 (MQ=255)

                                                                                           aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaa       >  1:2309916/1‑100 (MQ=255)

                                                                                           aGGCAGAGTTATCTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaa       >  2:2329314/1‑100 (MQ=255)

                                                                                           aGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaa       >  2:2013411/1‑100 (MQ=255)

                                                                                            ggCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGaaa      >  2:256950/1‑100 (MQ=255)

                                                                                              cAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAata    >  2:1969990/1‑100 (MQ=255)

                                                                                                gagaTAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATc  <  1:1971834/100‑1 (MQ=255)

TGTAAAGGGGGAGGTGGAAATAGCAATGAGGAGTATCAGCAAGAATACTCGCCGCTTTACCACAACGTGGATGAGAGGGATGAAAAACTCAAGGCAGAGATAACTCTGCCTTGAAGATAAATGCGCTTTTACAGCGGGCTTATTTCAGCTCTTCTGCTTCCGGTAAGGTCACGTTCAGCTCAAGAATAGAAATATC  >  NC_000913/1224140‑1224335

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`