BRESEQ :: Evidence

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,706,028	0	T	C	22.6%	396.2 / 115.1	213	intergenic (‑238/+70)	dppB/dppA	dipeptide/heme ABC transporter permease/dipeptide/heme ABC transporter periplasmic binding protein; dipeptide chemotaxis receptor
Reads supporting (aligned to +/- strand): ref base T (96/62); new base C (44/2); total (148/65)
Fisher's exact test for biased strand distribution p-value = 1.24e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.85e-01

Only 100 of 220 total aligned reads displayed.

ATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATG  >  NC_000913/3705930‑3706127

ntaCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGACCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑At‑t‑‑‑t                                                                                                      >  2:1656929/2‑100 (MQ=255)

 taCAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑At‑t‑‑‑tt                                                                                                     <  1:2824942/100‑1 (MQ=255)

   cAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑g                                                                                                 <  1:139991/100‑1 (MQ=255)

        tgttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGc                                                                                            >  2:1267205/1‑100 (MQ=255)

         gttgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGcc                                                                                           <  1:802707/100‑1 (MQ=255)

           tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTg                                                                                         >  1:1440517/1‑100 (MQ=255)

           tgACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTg                                                                                         >  1:538996/1‑100 (MQ=255)

            gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGa                                                                                        >  2:1433147/1‑100 (MQ=255)

            gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGa                                                                                        >  1:23376/1‑100 (MQ=255)

            gACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGa                                                                                        <  1:575699/100‑1 (MQ=255)

             aCAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGAt                                                                                       >  1:715506/1‑100 (MQ=255)

               aaaGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATaa                                                                                     >  1:919992/1‑100 (MQ=255)

                 aGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGc                                                                                   >  2:1145561/1‑100 (MQ=255)

                  gTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCg                                                                                  <  2:1483982/100‑1 (MQ=255)

                      ncTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgc                                                                              >  1:2018889/2‑100 (MQ=255)

                      gcTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgc                                                                              <  2:47467/100‑1 (MQ=255)

                       cTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcg                                                                             >  1:1461731/1‑100 (MQ=255)

                       cTTAGTTTAGGCCGGATGCGGCGTTAACGCCTTAAACGGCCTAC‑CAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAg                                                                               <  2:2530563/98‑1 (MQ=18)

                        tttGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTCCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAgcgc                                                                            <  1:1510908/100‑1 (MQ=255)

                        atttcgtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑GAAATCGTGCTAATTCAATATATTGTG‑GG‑CT‑C‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAgcgc                                                                            >  1:2896208/10‑100 (MQ=18)

                          tGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCAt                                                                          <  1:840893/100‑1 (MQ=255)

                            tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAATTC‑TGCAAATTCAACAAATTGC‑‑GA‑ATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                                                                       >  1:2423937/6‑100 (MQ=18)

                            tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAATTC‑TGCAAATTCAACAAATTGC‑‑GA‑ATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                                                                       >  2:34661/6‑100 (MQ=18)

                            tgtaggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GA‑AA‑C‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCa                                                                        >  2:1101117/6‑100 (MQ=18)

                             gtaggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑GAAATCGTGCTAATTCAATATATTGTG‑GG‑CT‑C‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAg                                                                       >  1:2964782/5‑100 (MQ=18)

                              taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑GAAATCGTGCTAATTCAATATATTGTG‑GG‑CT‑C‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAgg                                                                      >  2:2779604/4‑100 (MQ=21)

                              taggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑‑AAATCATGCAAATCCAAAATATTGCA‑GG‑AG‑T‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                     >  2:1565302/4‑100 (MQ=21)

                               aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAAGCGTGCAAATTCAATACATTGCATGG‑GC‑C‑‑‑AT‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                     >  1:1799599/3‑100 (MQ=17)

                               aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAAGCATGCAAATTCAATATATTGCA‑GA‑GT‑T‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                     >  2:1255125/3‑100 (MQ=17)

                               aggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAACAGTGCAAATTCAATATATTGCA‑AT‑CT‑T‑‑‑CTG‑‑GTAGGCCTGATAAGCATCGCGCATCAGGc                                                                     >  2:2975724/3‑100 (MQ=18)

                               aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GG‑AC‑C‑‑‑AT‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                     >  2:1003199/3‑100 (MQ=18)

                               aggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATCATGAAAATTCAATACATTGCA‑AG‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGc                                                                     >  1:609550/3‑100 (MQ=17)

                                ggctggataaggcgTTCACGCCGCATCCAGCCTAC‑AAAATTGTGCAAAATCAATGGATTGCA‑CA‑GC‑T‑‑‑AGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                    >  2:189451/11‑100 (MQ=25)

                                ggcaggataaggcgTTCACGCCGCATCCGGCCTAC‑AAAAGCACGCAAATTCAATATATTGCA‑GA‑GA‑T‑‑‑GGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                    >  2:2406341/11‑100 (MQ=1)

                                ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAATTC‑TGCAAATTCAACAAATTGC‑‑GA‑ATCC‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt                                                                   >  1:160963/2‑100 (MQ=18)

                                ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GA‑AA‑C‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                    >  1:2546412/2‑100 (MQ=18)

                                ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑GA‑AA‑C‑‑‑CTT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                    >  1:2624815/2‑100 (MQ=18)

                                ggCCGGATAAGGCGTTTACGCCGCATCCGGCCTAC‑AAAATCGTGCTAATTCAATATATTGCA‑AA‑AA‑C‑‑‑CCT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                    >  2:2040094/2‑100 (MQ=17)

                                ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAGATCGTGCAAATTCAATATATTGCA‑TG‑AG‑C‑‑‑CAC‑‑GTAGGCCTGATAAGATTAGCGCATCAGGCa                                                                    >  1:885708/2‑100 (MQ=17)

                                ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATCGCTTAAATTCAATATATTGCA‑CC‑AT‑C‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                    >  1:1886256/2‑100 (MQ=21)

                                ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAACTTTGCAAATTCAATATATTGCA‑AT‑CT‑C‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                    >  2:2946719/2‑100 (MQ=18)

                                ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAACTGTGCAAATTCAATATATTGCA‑GG‑AA‑A‑‑‑CAC‑‑GTAGGCCTGATAAGCGAAGCGCATCAGGCa                                                                    >  1:493374/2‑100 (MQ=18)

                                ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAAACCTGCAAATTCAATATATTGCA‑GG‑AT‑A‑‑‑CGT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCa                                                                    >  1:1840588/2‑100 (MQ=17)

                                ggCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑‑AAATCATGCAAATCCAAAATATTGCA‑GG‑AG‑T‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAt                                                                   >  1:2371541/2‑100 (MQ=21)

                                 gCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                                                                   <  2:2670219/100‑1 (MQ=255)

                                 gCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCAt                                                                   >  2:2395223/1‑100 (MQ=255)

                                 gCCGGATGCGGCGATAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑CTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGACa                                                                    >  2:667640/1‑99 (MQ=255)

                                 gCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑AAAATCGCTTAAATTCAATATATTGCA‑CC‑AT‑C‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaa                                                                   >  2:2927051/1‑99 (MQ=21)

                                 gCCGGATAAGGCGTTCACGCCGCATCCGGCCTAC‑‑AAATCATGCAAATCCAAAATATTGCA‑GG‑AG‑T‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                                                                  >  2:1281867/1‑100 (MQ=21)

                                  ccggataaggcgTTCACGCCGCATCCGGCCTACAAAAATCTTGCCAATTCAATATATTGCA‑GG‑AC‑C‑‑‑AT‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagt                                                                  <  2:481022/92‑3 (MQ=18)

                                  ccggataaggcgTTCACGCCGCATCCGGCCTAC‑AAAACTTTGCAAATTCAATATATTGC‑‑‑A‑AT‑C‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaat                                                                  >  1:2206618/9‑98 (MQ=18)

                                  ccggataaggcgTTCACGCCGCATCCGGCCTAC‑AAAACTTTGCAAATTCAATATATTGC‑‑‑A‑AT‑C‑‑‑TCCGTGTAGGCCTGATAAGCGTAGCGCATCAGGCaat                                                                  >  1:2078737/9‑98 (MQ=18)

                                  caggataaggcgTTCACGCCTTATCCTGCCTAC‑AAAAGCACGCAAATTCAATATATTGCA‑GA‑GA‑T‑‑‑GGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAtt                                                                  >  1:2759267/9‑100 (MQ=14)

                                   cggataaggcgTTCACGCCGCATCCGGCCTAC‑AAAAGCACGCAAACTCAATATATTGCA‑GAGAT‑C‑‑‑AT‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaatt                                                                 >  1:358444/8‑97 (MQ=17)

                                   cggataaggcgTTCACGCCGCATCCGGCCTAC‑‑AAATCATGCAAATCCAAAATATTGCA‑GG‑AG‑T‑‑‑TAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAttta                                                                >  2:770288/8‑99 (MQ=21)

                                    ggataaggcgTTTACGCCGCATCCGGCCTAC‑AAAAGCATGCAAATTCAATATATTGCA‑GA‑GT‑T‑‑‑CAC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAgttt                                                                >  1:393530/7‑100 (MQ=17)

                                    ggataaggcgTTCACGCCGCATCCGGCCTAC‑AAAACCTTGCTAATTCAATATATTGCA‑GG‑GA‑C‑‑‑TAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagctt                                                                >  1:1650576/7‑96 (MQ=21)

                                     gataaggcgTTTACGCCGCATCCGGCCTAC‑AAAAATGTGCAAATTCAATAAATTGC‑‑‑A‑AT‑TCAACTT‑‑GTAGGCCTGATAAGCGCAGCGCATCAGGCAattt                                                                >  1:1569875/6‑100 (MQ=21)

                                     gataaggcgTTCACGCCGCATCCGGCCTAC‑AAAATCGCTTAAATTCAATATATTGCA‑CC‑AT‑C‑‑‑CTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCaattct                                                               >  1:1123783/6‑95 (MQ=25)

                                     gataaggcgTTCACGCCGCATCCGGCCTAC‑AAAACCTTGCTAATTCAATATATTGCA‑GG‑GA‑C‑‑‑TAT‑‑GTAGGCCTGATAAGCATAGCGCATCAGGCagcttt                                                               >  2:1340591/6‑95 (MQ=18)

                                      aTGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttg                                                              <  1:2539529/100‑1 (MQ=255)

                                      aTGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAACACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGTCATTtttg                                                              <  2:603550/100‑1 (MQ=255)

                                       tgaggcgTTTACGCCGCATCCGGCCTAC‑AAATTCTTCCAAATTCAATATATTGCA‑GA‑AA‑T‑‑‑CAT‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAattttta                                                             >  1:2058254/4‑98 (MQ=21)

                                       taaggcgTTCACGCCGCATCCAGCCTAC‑AAAATTGTGCAAAATCAATGGATTGCA‑CA‑GC‑T‑‑‑AGC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCagatttgc                                                             >  2:1828979/4‑93 (MQ=37)

                                       tGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgc                                                             >  2:2830722/1‑100 (MQ=255)

                                        aaggcgTTCACGCCGCATCCGGCCTAC‑AAAATCATGAAAATTCAATACATTGCA‑AG‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCacgctcgca                                                            >  2:1642562/3‑92 (MQ=18)

                                          ggcgTTCACGCCGCATCCGGCCTAC‑AAAAACATGCAAATTCAATAGATTGCA‑GAGAT‑T‑‑‑AT‑‑‑GTAGGCCTGATAAGCGTAGCGCATCAGGCAATtttgcgtt                                                          >  1:2113271/1‑97 (MQ=17)

                                          ggcgTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctt                                                          >  1:522768/1‑100 (MQ=255)

                                             gTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc                                                       <  2:2439778/100‑1 (MQ=255)

                                             gTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTtttgcctttgc                                                       <  2:2219190/100‑1 (MQ=255)

                                               tAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCa                                                     >  1:2740386/1‑100 (MQ=255)

                                                  cGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCa                                                  <  1:317525/100‑1 (MQ=255)

                                                     cTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTc                                               >  1:2461252/1‑100 (MQ=255)

                                                       tATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCtt                                             <  2:3027053/100‑1 (MQ=255)

                                                       tATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCtt                                             >  1:3027080/1‑100 (MQ=255)

                                                          ccGGCCTAT‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTa                                          >  1:2836218/1‑100 (MQ=255)

                                                          ccGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTa                                          >  1:1701931/1‑100 (MQ=255)

                                                           cGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTAt                                         <  1:849013/100‑1 (MQ=255)

                                                               cTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCt                                     >  2:43908/1‑100 (MQ=255)

                                                                tAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtt                                    >  1:588983/1‑100 (MQ=255)

                                                                 aC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGTCTTTGCCATCAGTCTTGTATGGCttt                                   >  2:497848/1‑100 (MQ=255)

                                                                  c‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCtttt                                  <  1:1325530/100‑1 (MQ=255)

                                                                    aaaaTCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTa                                 <  2:191995/100‑1 (MQ=255)

                                                                      aaTCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAat                               <  1:963388/100‑1 (MQ=255)

                                                                      aaTCGTGCAAATTCAATACATTGCA‑GA‑AT‑C‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTGTa                                 <  2:855359/98‑1 (MQ=255)

                                                                           tGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTc                          <  1:1145571/100‑1 (MQ=255)

                                                                            gCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCg                         <  2:23375/100‑1 (MQ=255)

                                                                               aaTTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCGTCAGGCATTTTTGCCTTTGCCATCAGTATTTTATGGCTTTTAATTATTCGATa                      >  1:631117/1‑100 (MQ=255)

                                                                                aTTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATag                     <  2:1634567/100‑1 (MQ=255)

                                                                                aTTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATag                     >  1:1743539/1‑100 (MQ=255)

                                                                                   cAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAgaga                  >  1:1987479/1‑100 (MQ=255)

                                                                                   cAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAgaga                  <  1:1023507/100‑1 (MQ=255)

                                                                                      tACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGt               >  1:686296/1‑100 (MQ=255)

                                                                                            gCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGa         >  2:2123683/1‑100 (MQ=255)

                                                                                            gCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGa         >  1:1046413/1‑100 (MQ=255)

                                                                                              a‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAg       <  2:792658/100‑1 (MQ=255)

                                                                                                gA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGt      >  2:1055707/1‑100 (MQ=255)

                                                                                                 a‑aT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTg     <  2:2724676/100‑1 (MQ=255)

                                                                                                    t‑t‑‑‑ttC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGAt   >  2:569830/1‑100 (MQ=255)

                                                                                                      t‑‑‑ttC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATg  <  1:1192502/100‑1 (MQ=255)

ATACAGATTGTTGACAAAGTGCGCTTTGTTTATGCCGGATGCGGCGTTAACGCCTTATCCGGCCTAC‑AAAATCGTGCAAATTCAATACATTGCA‑GA‑AT‑T‑‑‑TTC‑‑GTAGGCCTGATAAGCGGAGCGCATCAGGCATTTTTGCCTTTGCCATCAGTCTTGTATGGCTTTTAATTATTCGATAGAGACGTTTTCGAAGTGATG  >  NC_000913/3705930‑3706127

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`