Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,781,152	0	A	G	21.1%	176.7 / 16.0	95	intergenic (+135/‑63)	lldD/trmL	L‑lactate dehydrogenase, FMN‑linked/tRNA Leu mC34,mU34 2'‑O‑methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base A (47/28);  new base G (2/18);  total (49/46)
Fisher's exact test for biased strand distribution p-value = 2.58e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.94e-01

GGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGA  >  NC_000913/3781053‑3781243                                                                                                          |                                                                                             gggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑Ga                                                                                              >  2:1964002/1‑100 (MQ=255)  ggAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAg                                                                                             <  1:93270/100‑1 (MQ=255)   gagaGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAgg                                                                                            <  1:2612107/100‑1 (MQ=255)     gagGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAgggg                                                                                          <  1:2778207/100‑1 (MQ=255)      agGGGACCGTTCGGCGCTGTATGCACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑a                                                                                        <  1:856368/100‑1 (MQ=255)       ggggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑aa                                                                                       >  1:1203314/1‑100 (MQ=255)       ggggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑aa                                                                                       >  1:849270/1‑100 (MQ=255)         ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑TCTCCTTG‑CCCCTCC‑CGGG‑TGAGGGCCGGGGG‑GGGGGG‑gaaa                                                                                     >  1:2255885/1‑100 (MQ=255)         ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑aaaa                                                                                     >  1:2017473/1‑100 (MQ=255)         ggACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑aaaa                                                                                     <  2:1886315/100‑1 (MQ=255)          gACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAg                                                                                    >  1:192169/1‑100 (MQ=255)           aCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAgg                                                                                   >  2:321491/1‑100 (MQ=255)           aCCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAgg                                                                                   <  1:1964020/100‑1 (MQ=255)           aCCGTTCGGCGCTGTATGTACTCCCCCCCCCTGAAACGGCACCGCACTC‑TTTTTGT‑CTCCCTAG‑ACCGACC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAgg                                                                                   <  2:2866474/100‑1 (MQ=255)            ccGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGc                                                                                  >  1:1663671/1‑100 (MQ=255)            ccGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGc                                                                                  <  1:2060839/100‑1 (MQ=255)            ccGTTCGGCGCTGTATGTACTCCCCCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGc                                                                                  <  1:2033296/100‑1 (MQ=255)              gTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCg                                                                                >  2:2805295/1‑100 (MQ=255)                 cGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTCTTTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCa                                                                              >  2:2871980/1‑100 (MQ=255)                 cGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCAc                                                                             >  1:888123/1‑100 (MQ=255)                 cGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCAc                                                                             >  2:1251389/1‑100 (MQ=255)                  ggCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACt                                                                            >  2:2233330/1‑100 (MQ=255)                  ggCGCTGTATGTACCCCCTCACTCGGAAACGACACCGCACTC‑TTTTTTC‑CCCCCCCC‑CCCCCCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCAc                                                                             <  2:180428/99‑1 (MQ=255)                  ggCGCGGAATGTACTCCCTCACGCTGAACCGACCCCGCACTT‑TTTTTTT‑CTCCCGCG‑CCCCTCC‑GGGG‑GGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCAc                                                                             <  1:1076511/99‑1 (MQ=37)                    cgcTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGc                                                                          >  1:74421/1‑100 (MQ=255)                        gtatgtaCTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCAc                                                                      >  1:295664/1‑100 (MQ=255)                          atgtaCTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCa                                                                    >  2:2150303/1‑100 (MQ=255)                            gtaCTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAAt                                                                  >  1:1790276/1‑100 (MQ=255)                            gtaCTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTACTCACCAAt                                                                  >  2:501496/1‑100 (MQ=255)                               cTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAAtttt                                                               >  1:1726623/1‑100 (MQ=255)                                tCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTc                                                              >  1:827612/1‑100 (MQ=255)                                cccccccctctAAAACAACCCCGCAC‑C‑TTTTTTT‑CCACCCCGCCCCCTCG‑GGGG‑AGGGGGCGGGGGG‑GGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTc                                                              <  1:30568/93‑1 (MQ=255)                                 ccctccctCTGAACCGACCCCGCCCTC‑TTTTTTT‑CCCCCCCG‑CCCC‑CC‑GGGG‑GGGGGGCCCGGGTGGAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTc                                                              <  2:1760606/99‑1 (MQ=255)                                 ccctccctCTAAACCGCCCCCGC‑CTT‑TTTTTTT‑CCCCCCCC‑CCCCCCC‑GGGGAAGGGGGCGGGGGT‑GGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCt                                                             <  1:1819183/100‑1 (MQ=255)                                 ccctccctCTAAAACGCCCCCCCCTTT‑TTTTTTT‑CCCCCCCC‑CCCCTCG‑GGGG‑AGGGGGCCGGGGG‑GGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTc                                                              <  1:1953591/99‑1 (MQ=255)                                  cccccctctGAAACGCCACGGCACTT‑TTTTTTG‑CCCCCTCC‑CCCCTCG‑GGGG‑AGAGGCCGGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTg                                                            <  2:919671/95‑1 (MQ=25)                                   cTCCCTCGGAAACGACCCCGCCCTC‑TTTTTGT‑CTCCCTCC‑CCCCTCG‑GGGG‑GGGGGGCGGGGGT‑GGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                           <  2:1605145/100‑1 (MQ=255)                                   cTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGCGGGCCGGGGT‑GGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                           >  1:1368371/1‑100 (MQ=255)                                     ccctctGAAACGACCCCGCATTC‑TTTTTTT‑CCCCCTCC‑CCCCTCG‑GGGG‑GGGGGGCGGGGGT‑GGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTa                                                         <  2:1198879/98‑1 (MQ=255)                                     cACTGTGAAACGCCCCCGCACTT‑TTTTTTT‑CCCCCTCG‑CCCCCCC‑GGGG‑AGAGGG‑CGGGGT‑GGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGc                                                           <  2:187608/98‑1 (MQ=255)                                       ctgcaaaaCGCCCCCCCCCTT‑TTTTTTC‑CCCCCCCG‑CCCCTCC‑GGGG‑AGGGGGCCGGGGG‑GGGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGc                                                           <  1:1449570/91‑1 (MQ=255)                                       ctctGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CACCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATc                                                       >  2:1627567/1‑100 (MQ=255)                                         ctGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCt                                                     >  2:1773972/1‑100 (MQ=255)                                          tGAAACGACACCGCACTC‑TTTTTTT‑TTTCCTTG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTACTCACCAATTTTCTGCTATCCt                                                     >  2:924481/1‑99 (MQ=38)                                          tGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                    >  1:2455347/1‑100 (MQ=255)                                          tGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                    >  1:740311/1‑100 (MQ=255)                                          tGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTg                                                    >  2:666317/1‑100 (MQ=255)                                           gAAACGACACCGCTCTC‑TTTTTTT‑CTCCCTCA‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGc                                                   >  1:2043032/1‑100 (MQ=255)                                            aaaCGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcc                                                  >  2:1949050/1‑100 (MQ=255)                                            aaaCGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcc                                                  >  2:2183996/1‑100 (MQ=255)                                             aaCGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc                                                 >  2:1265944/1‑100 (MQ=255)                                             aaCGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccc                                                 >  2:1445173/1‑100 (MQ=255)                                               cGACCCCCCTCTT‑TTTTTTT‑CCCCCCCC‑CCCCTCG‑GGGG‑AGGGGGCGGGGGT‑GGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                <  1:1949068/99‑1 (MQ=255)                                                 cccccGCCCTT‑TTTTTTT‑CCCCCCCC‑CCCCTCG‑GGGG‑GGGGGGCGGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGccccc                                               <  1:317129/95‑1 (MQ=255)                                                  ccccGCTCTC‑TTTTTTTCCCCCCCCC‑CCCCCCC‑GGGG‑GGAGGCCGGGGGT‑GGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGcccc                                                <  1:2872006/95‑1 (MQ=255)                                                  ccccGCACTC‑TTTTTGT‑CTCCCCCT‑CCCCCCC‑GGGG‑AGCGGCCTGGGTT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGc                                             <  2:1304575/97‑1 (MQ=255)                                                    ccGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCAc                                           >  1:1733623/1‑99 (MQ=255)                                                     ccgcctc‑tTTTTTT‑CCCCCCCC‑CCCCCCCGGGGG‑GGAGGCCCGGGGG‑GGGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCAc                                           <  2:74420/95‑1 (MQ=255)                                                       ccttc‑tTTTTTC‑CCCCCCCC‑CCCCCCC‑GGGG‑GGAGGG‑CGGGGT‑GGGGGGAAAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCAc                                           <  2:1517357/93‑1 (MQ=255)                                                       ccctc‑tTTTTTC‑CGCCCTCC‑CCCCGCG‑GGGG‑GGGGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACt                                          <  2:629626/95‑1 (MQ=255)                                                         ctc‑tTTTTTT‑CTTTCTTG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                                     >  2:68267/1‑100 (MQ=255)                                                          cttttttttt‑CTCCCTCC‑CCCCGCG‑GGGG‑AGAGGGCCGGGTT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCg                                              <  2:192166/88‑1 (MQ=38)                                                           c‑tTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                   >  2:2192909/1‑100 (MQ=255)                                                           c‑tTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                   >  1:2903263/1‑100 (MQ=255)                                                             ttttttt‑CCCCCTCG‑CCCCCCC‑GGGG‑GGAGGCCCGGGGT‑GGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggggg                                   <  2:695845/99‑1 (MQ=255)                                                             ttttttt‑CCCCCCCG‑CCCCTCG‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGc                                  <  2:2115140/100‑1 (MQ=255)                                                             ttttttt‑CCCCCCCC‑CCCCCCCGGGGG‑GGGGGGCGGGGGG‑GGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAggg                                     <  1:2184015/98‑1 (MQ=255)                                                               ttttt‑CCCCCCCG‑CCCCCCG‑GGGG‑AGAGGGCGGGGGG‑GGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAg                                <  2:952736/100‑1 (MQ=255)                                                                      tcCGTCG‑CCCCTCC‑GGGG‑GGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                          <  2:1603293/100‑1 (MQ=255)                                                                       ccccCG‑CCCCCCG‑GGGG‑AGGGGCCCGGGGG‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCt                          <  1:2740521/99‑1 (MQ=255)                                                                       cccTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                         >  1:1912668/1‑100 (MQ=255)                                                                       cccTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                         >  1:638415/1‑100 (MQ=255)                                                                       cccTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                         >  2:1408672/1‑100 (MQ=255)                                                                       cccTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTa                         >  2:757474/1‑100 (MQ=255)                                                                        ccgcG‑CCCCTCG‑GGGG‑GGAGGGCCGGGGT‑GGTGGG‑AATAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                        <  2:2043014/97‑1 (MQ=255)                                                                        ccTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                        >  1:2182433/1‑100 (MQ=255)                                                                        ccTCC‑CCCCCCG‑GGGG‑GGGGGGCGGGGGG‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                        <  1:1673293/100‑1 (MQ=255)                                                                         cccccccccccC‑GGGG‑GGGGGGGCGGGGG‑GGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaa                        <  1:405354/95‑1 (MQ=255)                                                                         cTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                       >  1:1718698/1‑100 (MQ=255)                                                                         cTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTaaa                       >  1:193005/1‑100 (MQ=255)                                                                              ccccTCC‑GGGG‑AGAGGGCCGGGGT‑GGGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAAc                      <  2:875511/97‑1 (MQ=255)                                                                              ccccTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                   >  1:541489/1‑100 (MQ=255)                                                                              ccccTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATc                   >  1:240891/1‑100 (MQ=255)                                                                                ccTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                 >  1:1279061/1‑100 (MQ=255)                                                                                  cccggggg‑AGGGGGCGGGGGA‑GGGAGT‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGt                 <  1:1126574/95‑1 (MQ=255)                                                                                   cc‑GGGG‑GGGGGGCGGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTAc               <  2:453081/99‑1 (MQ=255)                                                                                   cc‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt              >  1:2063344/1‑100 (MQ=255)                                                                                   cc‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACt              >  2:1545874/1‑100 (MQ=255)                                                                                       ggg‑AGGGGGCCGGGGT‑GAGGGA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTa           <  2:1974232/100‑1 (MQ=255)                                                                                        gg‑AGAGGGCCGGGGC‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc          <  2:2063325/100‑1 (MQ=255)                                                                                         g‑agaGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACg         >  2:2634682/1‑100 (MQ=255)                                                                                            gagGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGaa       <  2:125697/100‑1 (MQ=255)                                                                                              gggCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGa        <  2:1203303/97‑1 (MQ=255)                                                                                              gggCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAAcc     >  1:2175468/1‑100 (MQ=255)                                                                                              ggccgggggg‑gAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTAc          <  2:629571/90‑1 (MQ=255)                                                                                                gcgggggg‑gggggA‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAg   <  1:2233350/97‑1 (MQ=255)                                                                                                 ccGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGa  <  2:2087822/100‑1 (MQ=255)                                                                                                          |                                                                                             GGGAGAGGGGACCGTTCGGCGCTGTATGTACTCCCTCACTCTGAAACGACACCGCACTC‑TTTTTTT‑CTCCCTCG‑CCCCTCC‑GGGG‑AGAGGGCCGGGGT‑GAGGGG‑AAAAGGCCGCACTGCTCACCAATTTTCTGCTATCCTGCCCCCGCACTAAGGGGGCAGTATGCTAAACATCGTACTTTACGAACCAGA  >  NC_000913/3781053‑3781243

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑