Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 777,575 T→C F412L (TTC→CTC)  tolA → membrane anchored protein in TolA‑TolQ‑TolR complex

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913777,5750TC99.3% 478.3 / ‑4.8 135F412L (TTC→CTC) tolAmembrane anchored protein in TolA‑TolQ‑TolR complex
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (57/77);  total (58/77)
Fisher's exact test for biased strand distribution p-value = 4.30e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.43e-01

Only 100 of 135 total aligned reads displayed.

ACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAG  >  NC_000913/777478‑777668
                                                                                                 |                                                                                             
 ccTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCa                                                                                            >  1:141019/1‑100 (MQ=255)
   tGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCTAAACCACCAAGCCAGGCAGTATATGAAGTGCTCaaa                                                                                          <  1:2142701/100‑1 (MQ=255)
   tGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCaaa                                                                                          >  1:2543869/1‑100 (MQ=255)
   tGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCaaa                                                                                          >  1:853240/1‑100 (MQ=255)
    gAAGGTGGCGATCCCACACTTTGACAGACTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCaaaa                                                                                         >  2:21950/1‑100 (MQ=255)
       ggTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAAcg                                                                                      >  1:1077171/1‑100 (MQ=255)
         tGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAAcgcg                                                                                    <  2:2854589/100‑1 (MQ=255)
          ggCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACgcgc                                                                                   <  2:2305741/100‑1 (MQ=255)
           gCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGcc                                                                                  <  2:324710/100‑1 (MQ=255)
            cGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCa                                                                                 >  1:2114556/1‑100 (MQ=255)
                cccGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCACGCCAGGCAGTATATGAAGTGCTAAAAACCGCGCCATTgg                                                                             <  2:1769549/100‑1 (MQ=255)
                 ccGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGa                                                                            >  1:1959096/1‑100 (MQ=255)
                  cGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGAc                                                                           >  2:1940773/1‑100 (MQ=255)
                  cGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGAc                                                                           >  2:865841/1‑100 (MQ=255)
                     aCTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTc                                                                        <  1:1466516/100‑1 (MQ=255)
                     aCTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTc                                                                        <  2:1414294/100‑1 (MQ=255)
                     aCTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTc                                                                        >  2:2692883/1‑100 (MQ=255)
                      cTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCa                                                                       >  2:929152/1‑100 (MQ=255)
                       tttGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCaa                                                                      <  1:1752198/100‑1 (MQ=255)
                         tGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAAc                                                                    >  1:1159855/1‑100 (MQ=255)
                          gTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAAcc                                                                   >  2:1547245/1‑100 (MQ=255)
                            cAGGCTGCGTTGGGAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGt                                                                 >  1:2054314/1‑100 (MQ=255)
                            cAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGt                                                                 <  1:654665/100‑1 (MQ=255)
                            cAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGt                                                                 >  2:949486/1‑100 (MQ=255)
                             aGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTa                                                                >  1:1227360/1‑100 (MQ=255)
                             aGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTa                                                                <  1:2417128/100‑1 (MQ=255)
                             aGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTa                                                                <  2:1310374/100‑1 (MQ=255)
                              ggCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTaa                                                               >  1:2962899/1‑100 (MQ=255)
                                 tGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATcg                                                            <  2:836726/100‑1 (MQ=255)
                                  gCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATcgc                                                           >  1:1988273/1‑100 (MQ=255)
                                  gCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATcgc                                                           <  1:1291226/100‑1 (MQ=255)
                                  gCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATcgc                                                           >  2:1289104/1‑100 (MQ=255)
                                   cGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAGGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATcgcg                                                          <  2:1019674/100‑1 (MQ=255)
                                   cGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATcgcg                                                          >  1:2407015/1‑100 (MQ=255)
                                   cGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATcgcg                                                          >  2:1550714/1‑100 (MQ=255)
                                    gTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGa                                                         >  2:851369/1‑100 (MQ=255)
                                       ggCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGt                                                      <  1:1860646/100‑1 (MQ=255)
                                       ggCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGt                                                      >  2:2624143/1‑100 (MQ=255)
                                        gcagcaGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGtt                                                     >  1:1307933/1‑100 (MQ=255)
                                         cagcagCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGtt                                                     <  1:1573862/99‑1 (MQ=255)
                                         cagcagCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTg                                                    <  2:2543846/100‑1 (MQ=255)
                                         cagcagCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTg                                                    <  2:2754417/100‑1 (MQ=255)
                                           gcagcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGAc                                                  <  1:1084485/100‑1 (MQ=255)
                                           gcagcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGAc                                                  >  1:1422418/1‑100 (MQ=255)
                                           gcagcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGAc                                                  <  2:2206791/100‑1 (MQ=255)
                                           gcagcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGAc                                                  <  2:2280152/100‑1 (MQ=255)
                                           gcagcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGAc                                                  <  1:2849915/100‑1 (MQ=255)
                                           gcagcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGAc                                                  <  1:2861858/100‑1 (MQ=255)
                                              gcTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGt                                               >  2:205342/1‑100 (MQ=255)
                                                tAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTc                                             <  2:1227349/100‑1 (MQ=255)
                                                 aaaCTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCg                                            <  2:456560/100‑1 (MQ=255)
                                                 aaaCTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCg                                            <  2:141018/100‑1 (MQ=255)
                                                 aaaCTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCg                                            <  1:267403/100‑1 (MQ=255)
                                                  aaCTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCgg                                           >  1:2470833/1‑100 (MQ=255)
                                                    cTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGAc                                         >  2:2270717/1‑100 (MQ=255)
                                                     ttGCGAAGATCCGGAGACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACg                                        <  1:594044/99‑1 (MQ=255)
                                                        cGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTc                                     >  2:2169749/1‑100 (MQ=255)
                                                          aaGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCaa                                   >  1:1812680/1‑100 (MQ=255)
                                                            gATCCCTAAACCACCAAGCAAGGCAGTATATGAAGTGCTCAAAAGCTCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACa                                 <  2:2376530/100‑1 (MQ=255)
                                                            gATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACCGTCCGAACAGTCAACa                                 <  1:1813832/100‑1 (MQ=38)
                                                            gATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACCGTCCGAACAGTCAACa                                 <  2:2077080/100‑1 (MQ=38)
                                                              tCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACCGTCCGAACAGTCAACATc                               <  2:1105432/100‑1 (MQ=38)
                                                              tCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACCGTCCGAACAGTCAACATc                               <  1:555263/100‑1 (MQ=38)
                                                               cccGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCa                              >  2:2251067/1‑100 (MQ=255)
                                                               cccGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACCGTCCGAACAGTCAACATcg                              <  1:1723343/100‑2 (MQ=37)
                                                                 cggaaCCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACCGTCCGAACAGTCAACATcgcg                            <  1:2406656/97‑4 (MQ=25)
                                                                 cGAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACCGTCCGAACAGTCAACATcgcg                            <  1:511943/100‑4 (MQ=37)
                                                                  gAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGc                           <  2:2340961/100‑1 (MQ=255)
                                                                  gAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGc                           <  1:2846808/100‑1 (MQ=255)
                                                                  gAAACCACCAAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGc                           <  1:1761774/100‑1 (MQ=255)
                                                                     accaccAAGCCGTGCAGTATATGAAGTGCTCAAAAACGAGCCATTGGACTTCAAACCGTAATCGCGATGTTGACCGTCCGAACAGTCAACATcg                              <  1:21951/94‑2 (MQ=25)
                                                                      ccaccaAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCg                       >  2:1074075/1‑100 (MQ=255)
                                                                       caccaAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCgg                      <  2:1988255/100‑1 (MQ=255)
                                                                       caccaAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCgg                      <  2:1446463/100‑1 (MQ=255)
                                                                       caccaAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCgg                      >  1:990407/1‑100 (MQ=255)
                                                                        accaAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGt                     >  1:24186/1‑100 (MQ=255)
                                                                         ccaAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCg                       <  1:758822/97‑1 (MQ=255)
                                                                          caAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTg                   >  2:479510/1‑100 (MQ=255)
                                                                          caAGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTg                   <  2:420300/100‑1 (MQ=255)
                                                                            aGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTg                   <  1:2901894/98‑1 (MQ=255)
                                                                            aGCCAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGcc                 >  1:1071800/1‑100 (MQ=255)
                                                                               cAGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACg              >  1:861845/1‑100 (MQ=255)
                                                                                aGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACgg             <  1:865849/100‑1 (MQ=255)
                                                                                aGGCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACgg             <  1:605822/100‑1 (MQ=255)
                                                                                 ggCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACggg            >  1:840908/1‑100 (MQ=255)
                                                                                  gCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACgggg           <  1:1606050/100‑1 (MQ=255)
                                                                                  gCAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACgggg           <  2:296063/100‑1 (MQ=255)
                                                                                   cAGTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGt          <  1:2972579/100‑1 (MQ=255)
                                                                                     gTATATGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTc        >  1:117623/1‑100 (MQ=255)
                                                                                      tataTGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCt       <  1:2546474/100‑1 (MQ=255)
                                                                                       atatGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTg      >  1:942422/1‑100 (MQ=255)
                                                                                        tatGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTgg     <  1:1500832/100‑1 (MQ=255)
                                                                                        tatGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTgg     >  1:2782561/1‑100 (MQ=255)
                                                                                        tatGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTgg     <  1:1500911/100‑1 (MQ=255)
                                                                                        tatGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTgg     <  2:2114538/100‑1 (MQ=255)
                                                                                        tatGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTgg     >  1:237435/1‑100 (MQ=255)
                                                                                         atGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGt    <  1:1408458/100‑1 (MQ=255)
                                                                                          tGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGCCTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGGTCTg      >  2:358198/1‑97 (MQ=255)
                                                                                          tGAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTa   <  1:1297484/100‑1 (MQ=255)
                                                                                           gAAGTGCTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAg  <  2:618917/100‑1 (MQ=255)
                                                                                                 |                                                                                             
ACCTGAAGGTGGCGATCCCGCACTTTGTCAGGCTGCGTTGGCAGCAGCTAAACTTGCGAAGATCCCGAAACCACCAAGCCAGGCAGTATATGAAGTGTTCAAAAACGCGCCATTGGACTTCAAACCGTAATCGCGATGTTGACTGTTCGGACGGTCAACATCAGGCACCGGTTGCCACGGGGTTCTGGTAG  >  NC_000913/777478‑777668

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: