BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	953,802	A→G	V222A (GTA→GCA)	focA ←	formate channel

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	953,802	0	A	G	97.7%	452.8 / ‑0.8	131	V222A (GTA→GCA)	focA	formate channel
Reads supporting (aligned to +/- strand): ref base A (2/1); new base G (63/65); total (65/66)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.00e-01

Only 100 of 131 total aligned reads displayed.

TTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTGT  >  NC_000913/953706‑953898

ttATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCaa                                                                                               <  1:46043/100‑1 (MQ=255)

 tgaccgTGATGAATTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAAt                                                                                              >  1:466153/6‑100 (MQ=255)

 tgaccgTGATGAATTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAAt                                                                                              >  2:2711730/6‑100 (MQ=255)

 tATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAAt                                                                                              >  1:2719127/1‑100 (MQ=255)

 tATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAAt                                                                                              >  1:1904440/1‑100 (MQ=255)

  aTCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATa                                                                                             >  2:2062220/1‑100 (MQ=255)

   tCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAc                                                                                            <  1:2198388/100‑1 (MQ=255)

   tCAGCGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAc                                                                                            <  1:508473/100‑1 (MQ=255)

    cAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAcc                                                                                           >  2:1131843/1‑100 (MQ=255)

     aGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATAccc                                                                                          <  2:1904423/100‑1 (MQ=255)

       tgatgaATTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCAt                                                                                        >  2:232225/1‑100 (MQ=255)

       tgatgaAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCAt                                                                                        >  1:2913383/1‑100 (MQ=255)

          tgaAATTCATCACGGCCAGGTGAGAAGAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCgg                                                                                     >  1:1846983/1‑100 (MQ=255)

            aaaTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGa                                                                                   >  1:128172/1‑100 (MQ=255)

             aaTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCGATACCCATCGGGAt                                                                                  >  2:2066458/1‑100 (MQ=255)

             aaTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGAt                                                                                  <  1:1131853/100‑1 (MQ=255)

             aaTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGAt                                                                                  <  2:760017/100‑1 (MQ=255)

              aTTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATc                                                                                 >  2:2510404/1‑100 (MQ=255)

               ttCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCa                                                                                <  2:2689816/100‑1 (MQ=255)

                tcatcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCAt                                                                               <  2:2215056/100‑1 (MQ=255)

                 catcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATa                                                                              <  1:2066477/100‑1 (MQ=255)

                  atcaCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATaa                                                                             >  1:1649057/1‑100 (MQ=255)

                    caCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATaaa                                                                            <  2:2370164/99‑1 (MQ=255)

                    caCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAAc                                                                           >  2:916621/1‑100 (MQ=255)

                    caCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAAc                                                                           <  2:18903/100‑1 (MQ=255)

                    caCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAAc                                                                           <  2:413198/100‑1 (MQ=255)

                     aCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGTAATACCCATCGGGATCATAAACa                                                                          >  2:2191615/1‑100 (MQ=255)

                     aCGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACa                                                                          >  1:2896835/1‑100 (MQ=255)

                       ggTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATg                                                                        <  1:665503/100‑1 (MQ=255)

                          cAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGttt                                                                     >  2:396831/1‑100 (MQ=255)

                          cAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGttt                                                                     >  1:2658713/1‑100 (MQ=255)

                           aGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTg                                                                    <  1:1060271/100‑1 (MQ=255)

                           aGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTg                                                                    <  1:1624753/100‑1 (MQ=255)

                            ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                   <  1:2285287/100‑1 (MQ=255)

                            ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                   >  1:2495017/1‑100 (MQ=255)

                            ggTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGc                                                                   <  1:1746222/100‑1 (MQ=255)

                             gTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCg                                                                  <  2:48358/100‑1 (MQ=255)

                               gagaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGAt                                                                <  2:861677/100‑1 (MQ=255)

                                agaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATa                                                               <  2:1971549/100‑1 (MQ=255)

                                 gaAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATAc                                                              >  2:734125/1‑100 (MQ=255)

                                 gaAAAATTTTCCGGTGCAGAACCGACTGCGGGCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGAt                                                                >  2:1198337/1‑98 (MQ=255)

                                   aaaaTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACtg                                                            <  2:1705710/100‑1 (MQ=255)

                                   aaaaTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACtg                                                            >  2:1832631/1‑100 (MQ=255)

                                    aaaTTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACtgt                                                           <  2:1295286/100‑1 (MQ=255)

                                         ttCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCa                                                      <  1:396836/100‑1 (MQ=255)

                                         ttCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCa                                                      <  1:1649132/100‑1 (MQ=255)

                                         ttCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCa                                                      >  1:2481928/1‑100 (MQ=255)

                                           ccGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCaaa                                                    <  1:485249/100‑1 (MQ=255)

                                             ggTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAAc                                                  >  1:1034865/1‑100 (MQ=255)

                                             ggTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAAc                                                  <  1:393497/100‑1 (MQ=255)

                                               tGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCAACGGGATCATAAACATATTTGCGATACTGTGCTCAAAACCg                                                >  1:2708170/1‑100 (MQ=255)

                                                gCAGAACTGACTGCGGTACAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGc                                               <  1:2145626/100‑1 (MQ=255)

                                                gCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGc                                               <  1:748613/100‑1 (MQ=255)

                                                gCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGc                                               >  2:2610756/1‑100 (MQ=255)

                                                  aGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTg                                             <  2:891976/100‑1 (MQ=255)

                                                  aGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTg                                             <  2:345365/100‑1 (MQ=255)

                                                   gAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTgg                                            >  1:2460341/1‑100 (MQ=255)

                                                   gAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTgg                                            <  1:2325706/100‑1 (MQ=255)

                                                    aaCCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGc                                           >  2:690093/1‑100 (MQ=255)

                                                    aaCCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGc                                           <  2:2209949/100‑1 (MQ=255)

                                                     aCCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGca                                          <  2:1034855/100‑1 (MQ=255)

                                                      ccGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCATAACCGCTGGcaa                                         <  1:1992313/100‑1 (MQ=255)

                                                      cagACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGc                                           <  2:138358/96‑1 (MQ=255)

                                                         aCTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaacaa                                      <  2:720581/100‑1 (MQ=255)

                                                         aCTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGcaacaa                                      <  1:1832647/100‑1 (MQ=255)

                                                           tGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCaacaaac                                    <  1:1558324/100‑1 (MQ=255)

                                                           tGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCaacaaac                                    <  2:663970/100‑1 (MQ=255)

                                                              ggTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATc                                 >  2:1998937/1‑100 (MQ=255)

                                                              ggTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATc                                 >  2:2989240/1‑100 (MQ=255)

                                                               gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                                >  1:1583935/1‑100 (MQ=255)

                                                               gTCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcg                                <  1:2816678/100‑1 (MQ=255)

                                                                tCCAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcgc                               <  1:1198348/100‑1 (MQ=255)

                                                                 ccAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATcgcg                              >  2:2702920/1‑100 (MQ=255)

                                                                  cAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGa                             >  2:514955/1‑100 (MQ=255)

                                                                  cAAAATTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGa                             >  2:1692661/1‑100 (MQ=255)

                                                                  cAAAATTCCGGGGATGCGAAGTAGCGAATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCACTGGCAACAAACATcgcg                              >  2:1123597/1‑99 (MQ=255)

                                                                   aaaaTTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATTGCGAc                            <  2:2658690/100‑1 (MQ=255)

                                                                   aaaaTTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGAc                            >  1:2229040/1‑100 (MQ=255)

                                                                    aaaTTCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGAcc                           <  1:2062239/100‑1 (MQ=255)

                                                                       ttCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgc                        <  2:1613424/100‑1 (MQ=255)

                                                                       ttCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgc                        <  1:1979935/100‑1 (MQ=255)

                                                                        tCCGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgca                       <  2:2494995/100‑1 (MQ=255)

                                                                         ccGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgc                        <  2:2910602/98‑1 (MQ=255)

                                                                          cGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgcagc                     <  2:774509/100‑1 (MQ=255)

                                                                          cGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgcagc                     >  2:1061904/1‑100 (MQ=255)

                                                                          cGGGGATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGgcagc                     >  1:983227/1‑100 (MQ=255)

                                                                              gATGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCa                 <  1:1968022/100‑1 (MQ=255)

                                                                               aTGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCAt                <  1:2033550/100‑1 (MQ=255)

                                                                                tGCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATa               >  2:3031165/1‑100 (MQ=255)

                                                                                tGCGAAGTCCCTCATTGCAATACCCATCGGGATCATAAACATGGTTGCGATACTGTGCACAAAACCGCTGGCAACAAACATCGCAACCgggaagcccata               >  2:795170/1‑90 (MQ=255)

                                                                                 gCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATaa              >  1:1105357/1‑100 (MQ=255)

                                                                                 gCGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATaa              <  1:233019/100‑1 (MQ=255)

                                                                                  cGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAAt             >  1:1525607/1‑100 (MQ=255)

                                                                                  cGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAAt             >  2:1355725/1‑100 (MQ=255)

                                                                                  cGAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAAt             >  2:2372228/1‑100 (MQ=255)

                                                                                   gAAGTCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATg            >  2:2906150/1‑100 (MQ=255)

                                                                                       tCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACg        <  1:1112704/100‑1 (MQ=255)

                                                                                       tCGCGGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACg        <  1:696836/100‑1 (MQ=255)

                                                                                        cgcgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGc       >  1:309494/1‑100 (MQ=255)

                                                                                         gcgGATTGCAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCt      >  1:2354926/1‑100 (MQ=255)

TTATCAGTGATGAAATTCATCACGGTCAGGTGAGAAAAATTTTCCGGTGCAGAACCGACTGCGGTCCAAAATTCCGGGGATGCGAAGTCGCGGATTACAATACCCATCGGGATCATAAACATGTTTGCGATACTGTGCTCAAAACCGCTGGCAACAAACATCGCGACCGGCAGCACCATAATGAACGCTTTGT  >  NC_000913/953706‑953898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`