Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,197,853 T→C N129D (AAT→GAT)  ymfE ← e14 prophage; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,197,8530TC98.7% 546.4 / ‑4.9 158N129D (AAT→GAT) ymfEe14 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (1/1);  new base C (64/91);  total (66/92)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 158 total aligned reads displayed.

GAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATA  >  NC_000913/1197756‑1197949
                                                                                                 |                                                                                                
gAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATCTAGATCGc                                                                                                >  1:1218472/1‑100 (MQ=255)
gAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGc                                                                                                >  2:54576/1‑100 (MQ=255)
    aTTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCAt                                                                                            <  1:1963790/100‑1 (MQ=255)
     ttCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCAtt                                                                                           >  1:301975/1‑100 (MQ=255)
     ttCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCAtt                                                                                           >  2:1510778/1‑100 (MQ=255)
      tCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTa                                                                                          <  1:2207043/100‑1 (MQ=255)
      tCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTa                                                                                          <  2:271962/100‑1 (MQ=255)
       cAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTAc                                                                                         <  1:555451/100‑1 (MQ=255)
       cAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTAc                                                                                         >  2:332446/1‑100 (MQ=255)
         aaaaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACAt                                                                                       >  2:379721/1‑100 (MQ=255)
         aaaaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACAt                                                                                       >  1:467235/1‑100 (MQ=255)
         aaaaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACAt                                                                                       >  1:665440/1‑100 (MQ=255)
          aaaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACAtt                                                                                      <  2:325824/100‑1 (MQ=255)
            aaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATtgt                                                                                    <  1:2242630/100‑1 (MQ=255)
             atatCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATtgtg                                                                                   <  2:277076/100‑1 (MQ=255)
             atatCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATtgtg                                                                                   >  1:2291545/1‑100 (MQ=255)
              tatCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGa                                                                                  <  1:2762232/100‑1 (MQ=255)
                tCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGc                                                                                >  2:539928/1‑100 (MQ=255)
                 ccAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCt                                                                               <  1:1590314/100‑1 (MQ=255)
                   aaaaaTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTc                                                                             <  2:1419649/100‑1 (MQ=255)
                   aaaaaTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTc                                                                             <  1:520657/100‑1 (MQ=255)
                   aaaaaTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTc                                                                             >  2:81139/1‑100 (MQ=255)
                    aaaaTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCa                                                                            <  2:313737/100‑1 (MQ=255)
                     aaaTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCAt                                                                           <  1:1400775/100‑1 (MQ=255)
                        tAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtt                                                                        <  2:1074271/100‑1 (MQ=255)
                          gTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCttta                                                                      <  1:906323/100‑1 (MQ=255)
                          gTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCttta                                                                      >  1:2703749/1‑100 (MQ=255)
                           tataCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttat                                                                     <  1:2955521/100‑1 (MQ=255)
                            ataCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttatt                                                                    <  2:2551326/100‑1 (MQ=255)
                             taCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttattt                                                                   <  2:2670654/100‑1 (MQ=255)
                             taCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttattt                                                                   <  2:89291/100‑1 (MQ=255)
                              aCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTTTTTTTTTTTGCAAATATAGATAGCTTATTATATTGTTTATTTCGTCtttatt                                                                    >  2:559633/1‑98 (MQ=255)
                                tttATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAAt                                                                >  2:865046/1‑100 (MQ=255)
                                 ttATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAAtt                                                               >  1:722720/1‑100 (MQ=255)
                                 ttATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAAtt                                                               <  1:620536/100‑1 (MQ=255)
                                  tATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAAttt                                                              <  1:896922/100‑1 (MQ=255)
                                  tATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAAttt                                                              <  2:301972/100‑1 (MQ=255)
                                   aTTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAAtttt                                                             <  2:2437642/100‑1 (MQ=255)
                                    ttCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTc                                                            <  2:1629506/100‑1 (MQ=255)
                                    ttCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTc                                                            <  2:621783/100‑1 (MQ=255)
                                     tCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCt                                                           <  1:1734784/100‑1 (MQ=255)
                                      ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGGGCTTCATCTTTATTTAATTTTCTg                                                          >  1:2463496/1‑100 (MQ=255)
                                      ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTg                                                          >  2:2197682/1‑100 (MQ=255)
                                      ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTATTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTg                                                          >  2:1627701/1‑100 (MQ=255)
                                       cAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCtct                                                         <  1:2058484/100‑3 (MQ=255)
                                        agaagaGTTCAATATAATGTTTGTCTTCATTTTTTCTTACTTCAGGGTAATATAGTTTGCTCATTACATTGTGAGATTCATCTTTATTTAATTTTCTGtt                                                        >  2:2108348/1‑100 (MQ=255)
                                         gaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTg                                                       >  2:1168926/1‑100 (MQ=255)
                                         gaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTg                                                       >  1:2504627/1‑100 (MQ=255)
                                         gaagaGTTCAATATAAGGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTg                                                       >  2:1171801/1‑100 (MQ=255)
                                              gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc                                                  <  1:1674432/100‑1 (MQ=255)
                                               ttCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc                                                  <  2:354108/99‑1 (MQ=255)
                                               ttCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCa                                                 <  1:1745042/100‑1 (MQ=255)
                                                 cAATATAATGTTTGTCTTCAATTTTTTTTTTTTTTTTTTAATATAGATCGCTCATTACATTTTGATCTTCATCTTTATTTAATTTTCTGTTTACTCCAg                                                >  2:850396/1‑99 (MQ=255)
                                                 cAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGc                                               <  1:2409305/100‑1 (MQ=255)
                                                     ataATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCtctc                                           >  2:221839/1‑100 (MQ=255)
                                                      taATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTcc                                          <  2:1946712/100‑1 (MQ=255)
                                                        aTGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGt                                        <  1:1270478/100‑1 (MQ=255)
                                                          gTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGa                                      >  1:1945828/1‑100 (MQ=255)
                                                           tttGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGAt                                     <  1:2323782/100‑1 (MQ=255)
                                                            ttGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATa                                    <  2:18653/100‑1 (MQ=255)
                                                              gTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAAc                                  >  2:1771388/1‑100 (MQ=255)
                                                                cTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACgg                                >  1:181430/1‑100 (MQ=255)
                                                                cTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACgg                                >  1:1779594/1‑100 (MQ=255)
                                                                 ttCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGt                               >  2:2434930/1‑100 (MQ=255)
                                                                 ttCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGt                               >  1:1087181/1‑100 (MQ=255)
                                                                 ttCAATTGTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGAATCTAGCTCTCCGTGATAACgg                                >  2:1589551/1‑99 (MQ=255)
                                                                  tCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGtt                              <  1:1911773/100‑1 (MQ=255)
                                                                   cAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGttt                             >  1:416755/1‑100 (MQ=255)
                                                                    aaTTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGtttt                            >  1:2575057/1‑100 (MQ=255)
                                                                     aTTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTta                           <  2:1555277/100‑1 (MQ=255)
                                                                      tttttCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtat                          <  1:1168937/100‑1 (MQ=255)
                                                                      tttttCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtat                          <  1:1171812/100‑1 (MQ=255)
                                                                      tttttCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTta                           <  1:1672657/99‑1 (MQ=255)
                                                                       ttttCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtata                         <  1:38812/100‑1 (MQ=255)
                                                                       ttttCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtata                         <  1:865054/100‑1 (MQ=255)
                                                                       ttttCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtata                         >  2:1777372/1‑100 (MQ=255)
                                                                       ttttCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtata                         <  2:1294843/100‑1 (MQ=255)
                                                                           cTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTa                     <  1:2147830/100‑1 (MQ=255)
                                                                           cTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTa                     >  1:1003000/1‑100 (MQ=255)
                                                                           cTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTa                     <  1:2771943/100‑1 (MQ=255)
                                                                           cTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTa                     >  2:133377/1‑100 (MQ=255)
                                                                             tACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGa                   <  1:2444692/100‑1 (MQ=255)
                                                                             tACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGa                   <  2:497279/100‑1 (MQ=255)
                                                                              aCTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGAt                  >  2:2770505/1‑100 (MQ=255)
                                                                               cTTCAGGGTAGTATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATg                 <  2:1989091/100‑1 (MQ=255)
                                                                               cTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATg                 >  2:837978/1‑100 (MQ=255)
                                                                               cTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATg                 >  2:3020282/1‑100 (MQ=255)
                                                                                ttCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGc                <  1:51557/100‑1 (MQ=255)
                                                                                 tCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCTTGATAACGTTTTTATAATAAGATGCt               >  2:2570373/1‑100 (MQ=255)
                                                                                   aGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTa             <  1:1575823/100‑1 (MQ=255)
                                                                                    gggTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTAt            >  2:2593551/1‑100 (MQ=255)
                                                                                      gTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATcc          <  1:936887/100‑1 (MQ=255)
                                                                                      gTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATcc          <  2:665433/100‑1 (MQ=255)
                                                                                         atatAGATCTCTCATTACATTGTGCGCTTCATCTTTATTTAATTTTCTGTTGACCCCAGCTCCCCGTGCTCACGGTTTTATAATTAGATCCTTATCCCaa       >  2:866037/1‑100 (MQ=255)
                                                                                          tataGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCaaa      <  1:2009590/100‑1 (MQ=255)
                                                                                          tataGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCaaa      >  2:158695/1‑100 (MQ=255)
                                                                                          tataGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCaaa      <  2:1816119/100‑1 (MQ=255)
                                                                                          nataGATCGCTCAATACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCaaa      >  2:1878913/2‑100 (MQ=255)
                                                                                              gATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGata  >  2:1138112/1‑100 (MQ=255)
                                                                                              gATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCAAGCTCTACGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGata  >  2:582931/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATA  >  NC_000913/1197756‑1197949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: