BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,255,337	T→A	S206C (AGT→TGT)	ycgV ←	putative adhesin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,255,337	0	T	A	97.4%	377.1 / 0.7	114	S206C (AGT→TGT)	ycgV	putative adhesin
Reads supporting (aligned to +/- strand): ref base T (1/2); new base A (57/53); total (59/55)
Fisher's exact test for biased strand distribution p-value = 6.12e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.80e-01

Only 100 of 114 total aligned reads displayed.

TGTAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGA  >  NC_000913/1255241‑1255432

tGTAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATcc                                                                                              >  2:2748263/1‑100 (MQ=255)

 gTAGCCCTGAACATCGATTGTCAGATCTGTAGCCGTAAAACGAGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACTTGTACATcta                                                                                             >  2:2170166/1‑98 (MQ=255)

    gCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCg                                                                                          >  1:2377580/1‑100 (MQ=255)

     cccTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCgg                                                                                         >  1:793927/1‑100 (MQ=255)

     cccTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCgg                                                                                         >  1:2408073/1‑100 (MQ=255)

     cccTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCgg                                                                                         >  2:425311/1‑100 (MQ=255)

      ccTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGt                                                                                        >  1:2298907/1‑100 (MQ=255)

      ccTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGt                                                                                        >  1:255607/1‑100 (MQ=255)

         gAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGt                                                                                     >  1:1574716/1‑100 (MQ=255)

         gAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGt                                                                                     >  2:296225/1‑100 (MQ=255)

            cATCGATTGTCAGGTCTGTAGCCTTAAACCGCGCAGCACCATTGGCGTTGTTGCCGTTGAGACCACCGATATAAAGACCTGTACATCCATCGGTc                                                                                       >  2:614513/1‑95 (MQ=255)

            cATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGAt                                                                                  >  2:1294091/1‑100 (MQ=255)

            cATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGAt                                                                                  <  2:2408051/100‑1 (MQ=255)

             aTCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGAtt                                                                                 <  1:1045616/100‑1 (MQ=255)

              tCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGAttt                                                                                <  1:2595612/100‑1 (MQ=255)

               cGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGAtttt                                                                               <  1:417751/100‑1 (MQ=255)

               cGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGAtttt                                                                               <  2:1170871/100‑1 (MQ=255)

                gATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTa                                                                              <  2:1707605/100‑1 (MQ=255)

                 aTTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTAc                                                                             >  1:520640/1‑100 (MQ=255)

                   tGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACtt                                                                           <  1:829595/100‑1 (MQ=255)

                    gTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTc                                                                          >  2:2051788/1‑100 (MQ=255)

                     tCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTcc                                                                         <  1:425315/100‑1 (MQ=255)

                      cAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCg                                                                        <  2:2854940/100‑1 (MQ=255)

                        ggTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCt                                                                      >  1:2507370/1‑100 (MQ=255)

                          tCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTc                                                                    <  1:367872/100‑1 (MQ=255)

                          tCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTc                                                                    >  1:2788988/1‑100 (MQ=255)

                            tGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCa                                                                  >  2:936985/1‑100 (MQ=255)

                            tGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCa                                                                  >  1:599778/1‑100 (MQ=255)

                             gTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCaa                                                                 >  2:468166/1‑100 (MQ=255)

                              tAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCaa                                                                 >  2:572352/1‑99 (MQ=255)

                                 ccGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACCTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATc                                                             >  1:2126360/1‑100 (MQ=255)

                                 ccGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATc                                                             >  1:128173/1‑100 (MQ=255)

                                    tAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGac                                                          >  2:2991279/1‑100 (MQ=255)

                                     aaaaCGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGaca                                                         >  2:1276785/1‑100 (MQ=255)

                                      aaaCGCGCAGCACCATTGGCGTTATTGCCGTTGCGACCCCCGATATACACACCTGTACATCCATCGGTCGTGCTTTTACTTCCGCTTCCAAGATCGaccc                                                        <  1:2419718/100‑3 (MQ=255)

                                        aCGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTTGTGATTATACGGCCGCTTCCGAGATCGACACTg                                                      <  2:2054806/100‑1 (MQ=255)

                                         cgcgCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTgg                                                     <  1:2748287/100‑1 (MQ=255)

                                         cgcgCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTgg                                                     >  1:145794/1‑100 (MQ=255)

                                          gcgcAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGt                                                    <  2:986597/100‑1 (MQ=255)

                                           cgcAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATACACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTg                                                   <  2:2126341/100‑1 (MQ=255)

                                           cgcAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTg                                                   <  1:1984077/100‑1 (MQ=255)

                                           cgcAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTg                                                   <  2:1839780/100‑1 (MQ=255)

                                           cgcAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTg                                                   <  2:592504/100‑1 (MQ=255)

                                             cagcaCCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGcc                                                 >  2:1115957/1‑100 (MQ=255)

                                                 aCCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAg                                             >  1:1138971/1‑100 (MQ=255)

                                                 aCCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAg                                             >  2:2473274/1‑100 (MQ=255)

                                                  ccATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGt                                            <  2:1582574/100‑1 (MQ=255)

                                                    aTTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCa                                          <  1:1706898/100‑1 (MQ=255)

                                                     ttGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCAt                                         <  1:189980/100‑1 (MQ=255)

                                                       ggCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTg                                       >  2:1282171/1‑100 (MQ=255)

                                                         cGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTTCGCTTCCAAGATCGACACTGGTGCCATATTCATTGAt                                     >  2:1649371/1‑100 (MQ=255)

                                                         cGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGACATAGTCATTGa                                      >  1:2736555/1‑99 (MQ=255)

                                                         cGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTATACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGAt                                     >  2:1649063/1‑100 (MQ=255)

                                                          gTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCCAGATCGACACTGGTGCCATAGTCATTGATg                                    >  2:2813778/1‑100 (MQ=255)

                                                          gTTATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATg                                    <  1:107666/100‑1 (MQ=255)

                                                           ttATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATgg                                   <  1:160008/100‑1 (MQ=255)

                                                            tATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGCTTTTAATTCAGCTTCCAAGACCCACACTGGTGCCATAGTTATTGATg                                    >  2:1759576/1‑98 (MQ=255)

                                                            tATTGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGt                                  >  1:2673424/1‑100 (MQ=255)

                                                              ttGCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTa                                >  2:2755884/1‑100 (MQ=255)

                                                                nccGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAgg                              >  1:601910/2‑100 (MQ=255)

                                                                gCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAgg                              >  1:1583136/1‑100 (MQ=255)

                                                                gCCGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAgg                              <  1:2123119/100‑1 (MQ=255)

                                                                  cGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGcc                            <  1:572359/100‑1 (MQ=255)

                                                                  cGTTGAGACCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGcc                            <  2:903188/100‑1 (MQ=255)

                                                                    ttGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCta                          <  2:1583122/100‑1 (MQ=255)

                                                                    ttGAGACCACCGATATAAACACCTGTACATCCTTCGGTCGTGATTTTACTTCCGCTTCCAAGATCTACACTGGTGCCATAGTCTTTGATGGTTAGGTCta                          >  2:759788/1‑100 (MQ=255)

                                                                       agaCCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATAc                       >  1:2662727/1‑100 (MQ=255)

                                                                        gaCCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATAcc                      <  1:296228/100‑1 (MQ=255)

                                                                        gaCCACCGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATAcc                      <  1:2935149/100‑1 (MQ=255)

                                                                          ccaccGATATAAACACCTGTACATCCATCGGTCGTGCTTTTACTTCCCCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCg                     >  2:2330497/1‑99 (MQ=255)

                                                                          ccaccGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGt                    >  2:752388/1‑100 (MQ=255)

                                                                          ccaccGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGt                    >  1:2357512/1‑100 (MQ=255)

                                                                          ccaccGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGt                    <  1:936993/100‑1 (MQ=255)

                                                                           caccGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGtt                   >  1:2051369/1‑100 (MQ=255)

                                                                            accGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTc                  <  1:1864422/100‑1 (MQ=255)

                                                                            accGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTc                  <  2:2843832/100‑1 (MQ=255)

                                                                             ccGATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCg                 <  2:2377558/100‑1 (MQ=255)

                                                                               gATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGa                <  2:2373957/99‑1 (MQ=255)

                                                                               gATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAg               >  2:1330662/1‑100 (MQ=255)

                                                                               gATATAAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAg               <  2:1574703/100‑1 (MQ=255)

                                                                                 tataAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGtt             >  1:207966/1‑100 (MQ=255)

                                                                                 tataAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGt              >  2:2826262/1‑99 (MQ=255)

                                                                                  ataAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGttt            <  2:793920/100‑1 (MQ=255)

                                                                                  ataAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGttt            <  2:2322107/100‑1 (MQ=255)

                                                                                  ataAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGttt            <  1:1294104/100‑1 (MQ=255)

                                                                                   taAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGtttt           >  2:2097511/1‑100 (MQ=255)

                                                                                   taAACACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGtttt           >  1:1321868/1‑100 (MQ=255)

                                                                                    aaaCACCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTc          <  1:26104/100‑1 (MQ=255)

                                                                                      acacCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCaa        <  2:914081/100‑1 (MQ=255)

                                                                                       cacCTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAAt       >  2:2399747/1‑100 (MQ=255)

                                                                                        acCTGTACATCCATCGGTCGTGATTTCACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATg      <  1:2264628/100‑1 (MQ=255)

                                                                                         ccTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATgg     <  1:2345529/100‑1 (MQ=255)

                                                                                         ccTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATGGATGGTTAGGCCTATACCGTTCGAGTTTTCAATgg     <  1:1372873/100‑1 (MQ=255)

                                                                                          cTGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATgg     >  2:2831274/1‑99 (MQ=255)

                                                                                           tGTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTg   >  1:928305/1‑100 (MQ=255)

                                                                                            gTACATCCATCGGTTGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTg   <  1:2991305/99‑1 (MQ=255)

                                                                                            gTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCaaagatcg  >  1:659857/1‑94 (MQ=255)

                                                                                            gTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTg   >  2:1483605/1‑99 (MQ=255)

                                                                                            gTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGa  <  1:2841747/100‑1 (MQ=255)

                                                                                            gTACATCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGa  <  1:11973/100‑1 (MQ=255)

TGTAGCCCTGAACATCGATTGTCAGGTCTGTAGCCGTAAAACGCGCAGCACCATTGGCGTTATTGCCGTTGAGACCACCGATATAAACACCTGTACTTCCATCGGTCGTGATTTTACTTCCGCTTCCAAGATCGACACTGGTGCCATAGTCATTGATGGTTAGGCCTATACCGTTCGAGTTTTCAATGGTGA  >  NC_000913/1255241‑1255432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`