Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,356,549 C→T A188T (GCT→ACT)  sapA ← antimicrobial peptide transport ABC transporter periplasmic binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,356,5490CT95.7% 350.3 / 4.0 117A188T (GCT→ACT) sapAantimicrobial peptide transport ABC transporter periplasmic binding protein
Reads supporting (aligned to +/- strand):  ref base C (4/1);  new base T (62/50);  total (66/51)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-01

Only 100 of 119 total aligned reads displayed.

ACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATC  >  NC_000913/1356453‑1356648
                                                                                                |                                                                                                   
aCTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGtata                                                                                                  >  1:2114245/1‑100 (MQ=255)
 cTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATaa                                                                                                 >  1:2358848/1‑100 (MQ=255)
 cTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATaa                                                                                                 >  2:2948572/1‑100 (MQ=255)
  tGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAAt                                                                                                <  1:1827004/100‑1 (MQ=255)
    aTACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATgg                                                                                              <  2:2064751/100‑1 (MQ=255)
    aTACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATgg                                                                                              <  1:1395918/100‑1 (MQ=255)
     tACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATggg                                                                                             <  1:2948598/100‑1 (MQ=255)
       cGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACAGGACCGAAGTATAATGGGtt                                                                                           >  2:2802182/1‑100 (MQ=255)
        ggACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTg                                                                                          <  2:518376/100‑1 (MQ=255)
          aCCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCg                                                                                        <  1:1036081/100‑1 (MQ=255)
          aCCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCg                                                                                        <  2:1736388/100‑1 (MQ=255)
             ggTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAgg                                                                                     <  1:2854969/100‑1 (MQ=255)
             ggTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAgg                                                                                     >  2:2378248/1‑100 (MQ=255)
               tGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTg                                                                                   >  2:841051/1‑100 (MQ=255)
               tGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTg                                                                                   >  1:118942/1‑100 (MQ=255)
                gCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGc                                                                                  <  2:2483262/100‑1 (MQ=255)
                 ccGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGcc                                                                                 >  1:923079/1‑100 (MQ=255)
                  cGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCca                                                                                <  1:293133/100‑1 (MQ=255)
                   gACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCcan                                                                               <  2:2194016/100‑2 (MQ=255)
                    aCCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCcaca                                                                              <  1:1327563/100‑1 (MQ=255)
                        gTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACaaaaa                                                                          <  1:270172/100‑1 (MQ=255)
                         ttGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAGTGGGTTGCGAGGTGCCACaaaaaa                                                                         <  1:2228570/100‑1 (MQ=255)
                          tGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAaag                                                                        >  2:1949926/1‑100 (MQ=255)
                          tGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAaag                                                                        <  1:495625/100‑1 (MQ=255)
                          tGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAaag                                                                        <  1:472412/100‑1 (MQ=255)
                           gACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAaaga                                                                       <  2:2017596/100‑1 (MQ=255)
                           gACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAaaga                                                                       >  1:731405/1‑100 (MQ=255)
                             cGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAaagaag                                                                     <  1:2471689/100‑1 (MQ=255)
                             cGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAaagaag                                                                     >  2:1490473/1‑100 (MQ=255)
                             cGGTCGAGTTGCTCCTGGCGATCTTCTGTCTCTAACTTCCAGCCATATTCTGCCAACAAGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAaagaat                                                                     <  2:2006129/100‑2 (MQ=255)
                               gTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCa                                                                   >  1:1024341/1‑100 (MQ=255)
                                  gAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATcc                                                                >  2:2295247/1‑100 (MQ=255)
                                   aGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCg                                                               >  2:2741215/1‑100 (MQ=255)
                                    gTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGCCCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCgg                                                              >  1:851173/1‑100 (MQ=255)
                                     ttGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGc                                                             <  2:1259158/100‑1 (MQ=255)
                                      tGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCt                                                            >  1:1324019/1‑100 (MQ=255)
                                      tGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCt                                                            >  2:2544334/1‑100 (MQ=255)
                                       gCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCTGGCTg                                                           >  2:75988/1‑100 (MQ=255)
                                       gCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTg                                                           <  1:1291351/100‑1 (MQ=255)
                                          ccTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTgggn                                                        <  2:2092292/100‑2 (MQ=255)
                                          ccTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGc                                                        >  2:2474206/1‑100 (MQ=255)
                                          ccTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGc                                                        <  2:209557/100‑1 (MQ=255)
                                          ccTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGc                                                        >  1:411661/1‑100 (MQ=255)
                                          ccTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGc                                                        <  1:1368764/100‑1 (MQ=255)
                                               cGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTc                                                   >  2:2609987/1‑100 (MQ=255)
                                                gATCTTCTTTCTCTAACTTCCGTGCATAATCTGCCGACATGACCGAAGTATAATGGGTTGCGAAGAGCAAAAAAAAAGAAGCATCCGGCTGGGc                                                        <  2:2714567/94‑1 (MQ=255)
                                                  tcttctTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGa                                                <  2:2358827/100‑1 (MQ=255)
                                                     tctttctCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACt                                             <  2:2139555/100‑1 (MQ=255)
                                                         tctcTAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAAc                                         >  1:565320/1‑100 (MQ=255)
                                                          ctctAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACg                                        >  1:577293/1‑100 (MQ=255)
                                                          ctctAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACg                                        <  2:851165/100‑1 (MQ=255)
                                                           tctAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACgg                                       <  2:1324006/100‑1 (MQ=255)
                                                            ctAACTTCCGGGCATATTCTGCCGACATGACCGGAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGt                                      >  2:2435326/1‑100 (MQ=255)
                                                            ctAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGt                                      <  2:980679/100‑1 (MQ=255)
                                                            ctAACTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGt                                      >  1:410596/1‑100 (MQ=255)
                                                              aaCTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTAt                                    <  2:1501737/100‑1 (MQ=255)
                                                              aaCTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTAt                                    >  2:2455213/1‑100 (MQ=255)
                                                              aaCTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTAt                                    <  1:2550096/100‑1 (MQ=255)
                                                              aaCTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTAt                                    >  2:500901/1‑100 (MQ=255)
                                                              aaCTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTAt                                    >  2:1199413/1‑100 (MQ=255)
                                                              aaCTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTAt                                    <  1:168688/100‑1 (MQ=255)
                                                               aCTTCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGGGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATg                                   >  2:1503795/1‑100 (MQ=255)
                                                                cTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGa                                  >  1:2780092/1‑100 (MQ=255)
                                                                  tCCGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGAtt                                <  2:2674530/100‑1 (MQ=255)
                                                                    cGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGCGTCGGAACTCAACGGTATGATTAt                              >  1:589647/1‑100 (MQ=255)
                                                                    cGGGCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTAt                              >  2:1482662/1‑100 (MQ=255)
                                                                       gCATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCa                           >  2:553030/1‑100 (MQ=255)
                                                                        cATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAg                          >  1:754090/1‑100 (MQ=255)
                                                                        cATATTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAg                          >  1:2463356/1‑100 (MQ=255)
                                                                          tatTCTGCCGACATGACCGAAGTATACTGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGtt                        >  1:1843459/1‑100 (MQ=255)
                                                                          tatTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGtt                        <  1:2728337/100‑1 (MQ=255)
                                                                           atTCTGCCGACATGACCGAAGTATAATGGGTTGCTAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGCTTATCCAGttt                       <  2:1596937/100‑1 (MQ=255)
                                                                           atTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGttt                       >  2:451857/1‑100 (MQ=255)
                                                                           atTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGttt                       >  2:917353/1‑100 (MQ=255)
                                                                           atTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGttt                       >  2:2222528/1‑100 (MQ=255)
                                                                           atTCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGttt                       >  1:2893814/1‑100 (MQ=255)
                                                                           atTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGGGCCACCAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGtt                        >  2:2579406/1‑99 (MQ=255)
                                                                             tCTGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGc                     <  1:104662/100‑1 (MQ=255)
                                                                               tGCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCgg                   >  2:2820640/1‑100 (MQ=255)
                                                                                gCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGa                  >  2:236221/1‑100 (MQ=255)
                                                                                gCCGACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGa                  >  1:2173551/1‑100 (MQ=255)
                                                                                 ccGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGAc                 >  1:1839464/1‑100 (MQ=255)
                                                                                   gACATGACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGc               >  2:2591478/1‑100 (MQ=255)
                                                                                    aCATGACTGCAGTATACTGGGTTGCGAGGTGATAAAACAATGAAGCAGCCTGCTGGGCGACTCGGAACTCAACGGTATGATTTTCCTGTTTGc                     <  2:1767515/93‑1 (MQ=255)
                                                                                        gACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTc          >  2:245947/1‑100 (MQ=255)
                                                                                        gACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTc          >  1:1579826/1‑100 (MQ=255)
                                                                                        gACCGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTc          >  2:1057186/1‑100 (MQ=255)
                                                                                          ccGAAGTATAATGGGTTGCGAGGTGCCACACAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCAc        >  2:2077254/1‑100 (MQ=255)
                                                                                          ccGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCttctttt        >  1:1052680/1‑95 (MQ=255)
                                                                                          ccGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCAc        >  1:404759/1‑100 (MQ=255)
                                                                                          ccGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCAc        >  1:4047/1‑100 (MQ=255)
                                                                                          ccGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCAc        >  2:1322476/1‑100 (MQ=255)
                                                                                          ccGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCAc        >  1:1122657/1‑100 (MQ=255)
                                                                                           cGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACg       >  1:411889/1‑100 (MQ=255)
                                                                                           cGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACg       <  1:523256/100‑1 (MQ=255)
                                                                                           cGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACg       <  1:3028304/100‑1 (MQ=255)
                                                                                           cGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACg       <  1:2799875/100‑1 (MQ=255)
                                                                                           cGAAGTATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACg       >  2:1340220/1‑100 (MQ=255)
                                                                                            gAAGTATAATGGGTTGCGAGGTGCAACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCAc        <  2:558621/98‑1 (MQ=255)
                                                                                                cATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATc  >  2:2586488/1‑100 (MQ=255)
                                                                                                |                                                                                                   
ACTGATACGGACCGGTGCCGACCGGTTGACGGTCGAGTTGCTCCTGGCGATCTTCTTTCTCTAACTTCCGGGCATATTCTGCCGACATGACCGAAGCATAATGGGTTGCGAGGTGCCACAAAAAAGAAGCATCCGGCTGGGCGAGTCGGAACTCAACGGTATGATTATCCAGTTTGCGGACGCTTTTCACGTTATC  >  NC_000913/1356453‑1356648

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: