BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	216,844	C→T	E54K (GAA→AAA)	yaeF ←	putative lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	216,844	0	C	T	97.9%	465.7 / ‑1.7	140	E54K (GAA→AAA)	yaeF	putative lipoprotein
Reads supporting (aligned to +/- strand): ref base C (3/0); new base T (63/74); total (66/74)
Fisher's exact test for biased strand distribution p-value = 1.02e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01

Only 100 of 140 total aligned reads displayed.

AAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGT  >  NC_000913/216747‑216940

aaGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTgg                                                                                                <  2:1240954/100‑1 (MQ=255)

  gACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTg                                                                                              <  1:2440570/100‑1 (MQ=255)

    cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGaa                                                                                            >  2:240394/1‑100 (MQ=255)

    cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGaa                                                                                            >  1:2323560/1‑100 (MQ=255)

    cgcgGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGaa                                                                                            >  1:1922912/1‑100 (MQ=255)

        gATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGTGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAagag                                                                                        <  2:2550268/100‑1 (MQ=255)

          ttCCTAAGGGGGTTACCCGATGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGGGCTCAGGTGACGTTGTTTCTTTTGTTGATTGTTTGGTGAAAGAGcg                                                                                      <  2:2534951/100‑2 (MQ=255)

          ttCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCt                                                                                      <  2:1586254/100‑1 (MQ=255)

            ccAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCttt                                                                                    >  2:2279695/1‑100 (MQ=255)

              aaaTGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTg                                                                                   <  2:2311898/99‑1 (MQ=255)

              aaaTGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGa                                                                                  >  2:458662/1‑100 (MQ=255)

               aaTGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGAt                                                                                 <  1:1429799/100‑1 (MQ=255)

                aTGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATg                                                                                <  1:2526540/100‑1 (MQ=255)

                 tGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGc                                                                               <  1:2601672/100‑1 (MQ=255)

                   aGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTg                                                                             <  2:2905262/100‑1 (MQ=255)

                     gTTACCCCAAGGCTTTAGGAGAACAGCAAATCGCCTGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGa                                                                           <  2:1374757/100‑1 (MQ=255)

                     gTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGa                                                                           >  1:2451041/1‑100 (MQ=255)

                        aCCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTATGATGCTGGAAtt                                                                        <  2:472606/100‑1 (MQ=255)

                         ccccAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGGGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAAttt                                                                       >  1:2941779/1‑100 (MQ=255)

                         ccccAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAAttt                                                                       >  1:2805023/1‑100 (MQ=255)

                         ccccAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAAttt                                                                       <  1:239333/100‑1 (MQ=255)

                         ccccAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAAttt                                                                       >  1:1677555/1‑100 (MQ=255)

                         ccccAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAAttt                                                                       >  2:1511778/1‑100 (MQ=255)

                          cccAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTg                                                                      <  1:592653/100‑1 (MQ=255)

                           ccAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGa                                                                     >  1:883377/1‑100 (MQ=255)

                                gCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGcc                                                                >  1:958620/1‑100 (MQ=255)

                                 cTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGccc                                                               >  1:517474/1‑100 (MQ=255)

                                  ttGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCa                                                              <  2:2451019/100‑1 (MQ=255)

                                   tGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCAt                                                             >  1:2078527/1‑100 (MQ=255)

                                   tGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCAt                                                             >  1:2615080/1‑100 (MQ=255)

                                   ngaggagAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCACAt                                                             >  2:2781128/2‑100 (MQ=255)

                                    gaggagAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGATAGCCCATg                                                            >  2:2437702/1‑100 (MQ=255)

                                     aggagAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGt                                                           <  2:2594859/100‑1 (MQ=255)

                                      ggagAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGtt                                                          >  2:2356967/1‑100 (MQ=255)

                                        agaACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGtttt                                                        <  1:1882174/100‑1 (MQ=255)

                                         gaACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGccagatcggaa                                                       >  2:579743/1‑91 (MQ=255)

                                          aaCAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTg                                                      <  2:1411909/100‑1 (MQ=255)

                                          aaCAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTg                                                      <  2:2210842/100‑1 (MQ=255)

                                          aaCAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTg                                                      <  2:1355802/100‑1 (MQ=255)

                                          aaCAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTg                                                      <  2:1295793/100‑1 (MQ=255)

                                          aaCAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTg                                                      <  2:2210525/100‑1 (MQ=255)

                                           aCAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGc                                                     >  1:688670/1‑100 (MQ=255)

                                            cAGCAAATCGCCGGGTTTGAGATCGGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATTCTGGAATTTGACAGCCCATGATTTTg                                                      >  2:2918938/1‑98 (MQ=255)

                                            cAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGcc                                                    >  2:1607679/1‑100 (MQ=255)

                                            cAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGcc                                                    >  2:2205039/1‑100 (MQ=255)

                                             aGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCt                                                   >  2:366971/1‑100 (MQ=255)

                                               cAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCg                                                 >  1:428295/1‑100 (MQ=255)

                                                aaaTCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCgg                                                <  2:1965005/100‑1 (MQ=255)

                                                aaaTCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTCACAGCCCATGTTTTTGCCTCgg                                                <  1:2843329/100‑1 (MQ=255)

                                                  aTCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCa                                              <  2:525748/100‑1 (MQ=255)

                                                     gCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGGTTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCt                                           >  2:1497642/1‑100 (MQ=255)

                                                     gCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGTAATTTGACAGCCCATGTTTTTGCCTCGGCctct                                           >  1:1016709/1‑100 (MQ=255)

                                                     gCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCt                                           >  1:661213/1‑100 (MQ=255)

                                                       cGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTAc                                         >  1:1517182/1‑100 (MQ=255)

                                                        gggTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTAct                                        >  2:845726/1‑100 (MQ=255)

                                                          gTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTActgc                                      <  1:2322248/100‑1 (MQ=255)

                                                          gTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTActgc                                      <  2:1782955/100‑1 (MQ=255)

                                                          gTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGAGAAAGAGCTTTGATGCTGGAATTTGACAGCCAATGTTTTTGCTTCGGCATCt                                           >  2:1007308/1‑95 (MQ=255)

                                                           tttGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTActgct                                     >  1:1212717/1‑100 (MQ=255)

                                                            ttGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTActgctg                                    <  2:852342/100‑1 (MQ=255)

                                                             tGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCtgt                                   >  1:2794906/1‑100 (MQ=255)

                                                              gagaTCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCtgtg                                  >  2:2778821/1‑100 (MQ=255)

                                                               agaTCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTgg                                 <  1:600957/100‑1 (MQ=255)

                                                               agaTCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTgg                                 >  2:2225851/1‑100 (MQ=255)

                                                               agaTCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTgg                                 >  1:1640349/1‑100 (MQ=255)

                                                               agaTCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTgg                                 >  1:738894/1‑100 (MQ=255)

                                                                gaTCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGc                                <  2:1212706/100‑1 (MQ=255)

                                                                gaTCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGc                                <  1:2189598/100‑1 (MQ=255)

                                                                gaTCAGGTGCCGTTATTTCCTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGc                                >  2:2501851/1‑100 (MQ=255)

                                                                 aTCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCt                               <  1:1607693/100‑1 (MQ=255)

                                                                 aTCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCt                               >  2:1437344/1‑100 (MQ=255)

                                                                  tCAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTg                              >  1:1246027/1‑100 (MQ=255)

                                                                   cAGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGa                             <  1:845734/100‑1 (MQ=255)

                                                                    aGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGAt                            <  2:972304/100‑1 (MQ=255)

                                                                    aGGTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGAt                            >  2:1731846/1‑100 (MQ=255)

                                                                     ggTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATg                           <  2:1632577/100‑1 (MQ=255)

                                                                     ggTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATg                           >  1:1597173/1‑100 (MQ=255)

                                                                     ggTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATg                           >  2:1097271/1‑100 (MQ=255)

                                                                      gTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATgg                          <  1:2742033/100‑1 (MQ=255)

                                                                      gTGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATgg                          <  1:1071723/100‑1 (MQ=255)

                                                                       tGCCGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATggg                         <  1:1627644/100‑1 (MQ=255)

                                                                          cGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTct                      <  2:1846133/100‑1 (MQ=255)

                                                                          cGTTATTTCTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTct                      >  2:2786118/1‑100 (MQ=255)

                                                                                  cTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTggctggct              <  2:1922894/100‑1 (MQ=255)

                                                                                  cTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTggctggct              <  2:967180/100‑1 (MQ=255)

                                                                                  cTTTTATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTggctggct              >  2:841474/1‑100 (MQ=255)

                                                                                   ttttATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGgctggctg             <  2:2615057/100‑1 (MQ=255)

                                                                                   ttttATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGgctggctg             >  1:456397/1‑100 (MQ=255)

                                                                                    tttATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGa            <  1:1581833/100‑1 (MQ=255)

                                                                                     ttATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGat           <  1:2252965/100‑1 (MQ=255)

                                                                                      tATTGATTGTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGata          >  2:1099963/1‑100 (MQ=255)

                                                                                       attgattgTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGatat         <  1:2167415/100‑1 (MQ=255)

                                                                                        ttgattgTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATc        <  1:2383088/100‑1 (MQ=255)

                                                                                         tgattgTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCa       >  2:2109604/1‑100 (MQ=255)

                                                                                          gattgTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCaa      >  1:1006818/1‑100 (MQ=255)

                                                                                           attgTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAAc     >  2:1401222/1‑100 (MQ=255)

                                                                                           attgTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAAc     <  2:2108998/100‑1 (MQ=255)

                                                                                             tgTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACgg   <  1:1437356/100‑1 (MQ=255)

                                                                                              gTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGt  >  1:1390225/1‑100 (MQ=255)

                                                                                              gTTTGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGt  >  2:1048034/1‑100 (MQ=255)

AAGACGCGGATTCCAAATGAGGTTACCCCAAGGCTTGAGGAGAACAGCAAATCGCCGGGTTTGAGATCAGGTGCCGTTATTTCTTTTATTGATTGTTCGGTGAAAGAGCTTTGATGCTGGAATTTGACAGCCCATGTTTTTGCCTCGGCATCTACTGCTGTGGCTGATGGGTCTGGCTGGCTGATATCAACGGT  >  NC_000913/216747‑216940

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`