Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,613,799 T→C D302G (GAC→GGC)  sad ← succinate semialdehyde dehydrogenase, NAD(P)+‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,613,7990TC95.7% 379.8 / 1.4 115D302G (GAC→GGC) sadsuccinate semialdehyde dehydrogenase, NAD(P)+‑dependent
Reads supporting (aligned to +/- strand):  ref base T (4/1);  new base C (54/56);  total (58/57)
Fisher's exact test for biased strand distribution p-value = 3.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 115 total aligned reads displayed.

ACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGC  >  NC_000913/1613704‑1613893
                                                                                               |                                                                                              
aCAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACg                                                                                            >  1:2204821/1‑100 (MQ=255)
aCAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACg                                                                                            >  2:59030/1‑100 (MQ=255)
      gcgcACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATc                                                                                      >  2:1621007/1‑100 (MQ=255)
        gcACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGc                                                                                    >  2:451339/1‑100 (MQ=255)
         cACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGcc                                                                                   >  1:581518/1‑100 (MQ=255)
         cACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGcc                                                                                   <  2:3002967/100‑1 (MQ=255)
            ccTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCAt                                                                                <  1:1910106/100‑1 (MQ=255)
             cTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCAtt                                                                               >  1:2805954/1‑100 (MQ=255)
             cTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCAtt                                                                               >  2:2771107/1‑100 (MQ=255)
              tGCGGCAGAGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAGCGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCAttt                                                                              <  2:1885333/100‑1 (MQ=255)
               gcgcCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCAtttt                                                                             <  2:1284692/100‑1 (MQ=255)
                cgcCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTc                                                                            >  2:1949623/1‑100 (MQ=255)
                 gcCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCa                                                                           <  2:361791/100‑1 (MQ=255)
                  ccATGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCaa                                                                          >  2:1202856/1‑100 (MQ=255)
                   cAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAg                                                                         <  1:2328736/100‑1 (MQ=255)
                   cAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAg                                                                         <  1:2314320/100‑1 (MQ=255)
                   cAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAg                                                                         <  2:2170880/100‑1 (MQ=255)
                   cAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAg                                                                         <  2:2412685/100‑1 (MQ=255)
                   cAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAg                                                                         <  2:630044/100‑1 (MQ=255)
                    aGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAgg                                                                        >  2:762522/1‑100 (MQ=255)
                    aGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAgg                                                                        <  2:2237904/100‑1 (MQ=255)
                     gggTTTTCTCCACCCGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGc                                                                       <  2:2064403/100‑1 (MQ=255)
                       gTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAATGCTg                                                                     >  2:402551/1‑100 (MQ=255)
                         tttCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGcc                                                                   <  1:1627524/100‑1 (MQ=255)
                           tctcCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGc                                                                 <  1:1949641/100‑1 (MQ=255)
                           tctcCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGc                                                                 >  2:237948/1‑100 (MQ=255)
                           tctcCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATGTGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGc                                                                    <  2:1581160/97‑1 (MQ=255)
                            ctcCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCa                                                                <  2:2773464/100‑1 (MQ=255)
                             tcCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAg                                                               >  1:1449445/1‑100 (MQ=255)
                               cACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCt                                                             >  2:529692/1‑100 (MQ=255)
                                aCCTGATGATGCAGCTCATCACGTACATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATTGCCCATTTTCAAGGCTGCCGCAGCTg                                                            >  1:1985786/1‑100 (MQ=255)
                                 ccTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTg                                                            <  2:2310048/99‑1 (MQ=255)
                                 ccTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGc                                                           >  2:2773623/1‑100 (MQ=255)
                                 ccTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGc                                                           >  1:2858883/1‑100 (MQ=255)
                                  cTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGcc                                                          <  1:2944344/100‑1 (MQ=255)
                                   tgatgaTGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCca                                                         >  1:2956448/1‑100 (MQ=255)
                                    cttgatgCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCcac                                                        <  1:450978/98‑1 (MQ=255)
                                     atgatgCAGCTCGTCACATAGGCCAAAGCGGGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGcc                                                          <  2:1348088/97‑1 (MQ=255)
                                     atgatgCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCcaca                                                       <  2:2072530/100‑1 (MQ=255)
                                      tgatgCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCcacag                                                      >  2:450974/1‑99 (MQ=255)
                                        atgCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACaaaa                                                    >  2:160266/1‑100 (MQ=255)
                                        atgCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGACTGCCGCAGCTGCCACaaaa                                                    <  1:2851138/100‑1 (MQ=255)
                                         tgCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACACAAc                                                   >  1:2567434/1‑100 (MQ=255)
                                         tgCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAAc                                                   <  2:2204802/100‑1 (MQ=255)
                                          gCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACg                                                  >  1:510788/1‑100 (MQ=255)
                                          gCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACg                                                  <  1:571322/100‑1 (MQ=255)
                                            aGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGtt                                                >  1:2326038/1‑100 (MQ=255)
                                             gCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTc                                               <  2:2241928/100‑1 (MQ=255)
                                             gCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCACATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTc                                               >  1:2884492/1‑100 (MQ=255)
                                              cTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCg                                              >  1:246155/1‑100 (MQ=255)
                                                catcaCGTAAATCAAAACGAGCCATTGATCCGAGAGCGTTCTCTTCGCCACGGCGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCAGt                                            >  2:1471611/1‑100 (MQ=255)
                                                 atcaCGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTa                                           >  2:1152963/1‑100 (MQ=255)
                                                   caCGTTAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTaaa                                         >  2:3009953/1‑100 (MQ=255)
                                                    aCGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAAt                                        <  2:2937524/100‑1 (MQ=255)
                                                     cGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATg                                       >  1:1644117/1‑100 (MQ=255)
                                                     cGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATg                                       >  1:295071/1‑100 (MQ=255)
                                                     cGTAAATCAAAACGAGCCATTGGTCCGAGAGCGCTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATg                                       >  2:2220819/1‑100 (MQ=255)
                                                      ntAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGc                                      >  1:415436/2‑100 (MQ=255)
                                                       tAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGcc                                     <  1:2526942/100‑1 (MQ=255)
                                                        aaaTCAAACCGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCg                                    <  2:2884466/100‑1 (MQ=255)
                                                        aaaTCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCg                                    >  2:3008151/1‑100 (MQ=255)
                                                           tCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAg                                 <  2:2250771/100‑1 (MQ=255)
                                                           tCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAg                                 >  1:1146375/1‑100 (MQ=255)
                                                           tCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAg                                 >  1:1014468/1‑100 (MQ=255)
                                                            naaaaCGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTACCGCAGCTGCCACAAAACGTTCGGTAAATGCCGaa                                  >  2:949646/2‑98 (MQ=255)
                                                            cAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGc                                <  1:1534518/100‑1 (MQ=255)
                                                            cAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGc                                <  1:1885941/100‑1 (MQ=255)
                                                            cAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGc                                <  1:1944570/100‑1 (MQ=255)
                                                             aaaaCGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCa                               >  1:1243773/1‑100 (MQ=255)
                                                             aaaaCGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCa                               >  1:1333006/1‑100 (MQ=255)
                                                              aaaCGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAg                                 >  2:1674326/1‑97 (MQ=255)
                                                                 cGAGCCATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTc                           >  2:2548671/1‑100 (MQ=255)
                                                                     ccATTGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCctc                       <  1:1276554/100‑1 (MQ=255)
                                                                       aTTGGTCCGAGAGCGTTCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCtt                     <  1:1870188/100‑1 (MQ=255)
                                                                        ttGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTc                    <  1:2939686/100‑1 (MQ=255)
                                                                         tGGTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATGCCCTCTTCg                   <  2:525517/100‑1 (MQ=255)
                                                                          ggTCCGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGa                  <  2:497916/100‑1 (MQ=255)
                                                                             ccGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGataa               <  2:1032080/100‑1 (MQ=255)
                                                                              cGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGataat              >  1:534094/1‑100 (MQ=255)
                                                                              cGAGAGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGataat              >  1:840291/1‑100 (MQ=255)
                                                                               gagaGCGTTCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGataata             >  1:1542399/1‑100 (MQ=255)
                                                                               gagaGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGataata             >  2:589733/1‑100 (MQ=255)
                                                                               gagaGCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGataata             <  2:760230/100‑1 (MQ=255)
                                                                                agagCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGAtaataa            <  1:2284030/100‑1 (MQ=255)
                                                                                agagCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGAtaataa            <  1:1621021/100‑1 (MQ=255)
                                                                                  agCGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAg          <  2:134452/100‑1 (MQ=255)
                                                                                    cGTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCg        <  2:840284/100‑1 (MQ=255)
                                                                                     gTTCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGt       >  1:2691487/1‑100 (MQ=255)
                                                                                      ttCTCTTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGtt      >  1:165620/1‑100 (MQ=255)
                                                                                        ctctTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGTAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGtttt    >  2:1870321/1‑100 (MQ=255)
                                                                                        ctctTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGtttt    >  1:686753/1‑100 (MQ=255)
                                                                                        ctctTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGtttt    <  2:2956422/100‑1 (MQ=255)
                                                                                        ctctTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGtttt    <  2:1455187/100‑1 (MQ=255)
                                                                                        ctctTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGtttt    >  2:623745/1‑100 (MQ=255)
                                                                                         tctTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTg   >  1:2948503/1‑100 (MQ=255)
                                                                                         tctTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTg   <  1:529698/100‑1 (MQ=255)
                                                                                         tctTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTg   <  1:1131037/100‑1 (MQ=255)
                                                                                          ctTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGc  <  1:1635025/100‑1 (MQ=255)
                                                                                          ctTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGc  >  1:2241940/1‑100 (MQ=255)
                                                                                          ctTCGCCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGCCAAATGCCGAAGCAATCCCCCCTTCGATAATAAAGCGTTTCGc  >  2:2583118/1‑100 (MQ=255)
                                                                                               |                                                                                              
ACAAACGCGCACCCTGCGCCAGGGTTTTCTCCACCTGATGATGCAGCTCATCACGTAAATCAAAACGAGCCATTGGTCCGAGAGCGTTCTCTTCGTCACGGGGATCGCCCATTTTCAAGGCTGCCGCAGCTGCCACAAAACGTTCGGTAAATGCCGAAGCAATTCCCTCTTCGATAATAAAGCGTTTTGC  >  NC_000913/1613704‑1613893

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: