Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,087,983 T→C E92E (GAA→GAG yeeY ← LysR family putative transcriptional regulator

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,087,9830TC97.6% 433.0 / ‑0.7 125E92E (GAA→GAGyeeYLysR family putative transcriptional regulator
Reads supporting (aligned to +/- strand):  ref base T (2/1);  new base C (60/62);  total (62/63)
Fisher's exact test for biased strand distribution p-value = 6.19e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.97e-01

Only 100 of 125 total aligned reads displayed.

CAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCG  >  NC_000913/2087887‑2088078
                                                                                                |                                                                                               
cAGGCGCGTCACGCTATGATGTTGATAAAACGCTTCAATGAGTGGCACAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTcc                                                                                              >  2:2683104/1‑100 (MQ=255)
cAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTcc                                                                                              >  1:2555109/1‑100 (MQ=255)
 aGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTccc                                                                                             <  1:2320899/100‑1 (MQ=255)
 aGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTccc                                                                                             <  1:1664666/100‑1 (MQ=255)
  ggCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCa                                                                                            >  1:1250308/1‑100 (MQ=255)
     gcgTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGcc                                                                                         <  2:13797/100‑1 (MQ=255)
     gcgTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGcc                                                                                         <  2:12509/100‑1 (MQ=255)
       gTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCtt                                                                                       >  2:1794798/1‑100 (MQ=255)
       gTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCtt                                                                                       <  1:98253/100‑1 (MQ=255)
        tCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTc                                                                                      >  1:992245/1‑100 (MQ=255)
        tCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTc                                                                                      >  2:2105963/1‑100 (MQ=255)
        tCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTc                                                                                      >  1:1717541/1‑100 (MQ=255)
        tCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTc                                                                                      >  1:669869/1‑100 (MQ=255)
         cACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCa                                                                                     <  2:1695963/100‑1 (MQ=255)
         cACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCa                                                                                     <  1:1394452/100‑1 (MQ=255)
            gcaataatgTTTATAAAAGGCCTCAATGAGTGGAGAAAAAAAAAAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGc                                                                                  <  2:1061474/94‑1 (MQ=255)
            gCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGc                                                                                  <  2:2034458/100‑1 (MQ=255)
            gCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGc                                                                                  <  1:742726/100‑1 (MQ=255)
            gCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGc                                                                                  >  2:714599/1‑100 (MQ=255)
            gCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGc                                                                                  <  1:69634/100‑1 (MQ=255)
               atgatgTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCa                                                                                 >  2:1160023/1‑98 (MQ=255)
               atgatgTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGGCGTTCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGt                                                                               <  2:31752/100‑1 (MQ=255)
                ngatgTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGtt                                                                              >  2:1228310/2‑100 (MQ=255)
                 gatgTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGttt                                                                             <  1:1247844/100‑1 (MQ=255)
                 gatgTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGttt                                                                             <  2:1642858/100‑1 (MQ=255)
                 gatgTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGttt                                                                             <  1:816490/100‑1 (MQ=255)
                  atgTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTg                                                                            >  1:2433943/1‑100 (MQ=255)
                   tgTTGATAAAAAGCTTCAATGGGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCGCCCAGCCTTCATGCAGTTTg                                                                            <  2:166959/99‑1 (MQ=255)
                   tgTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGa                                                                           >  1:2066967/1‑100 (MQ=255)
                    gTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGAt                                                                          <  1:1924313/100‑1 (MQ=255)
                     ttGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATc                                                                         >  1:1100516/1‑100 (MQ=255)
                      tGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCg                                                                        <  2:743751/100‑1 (MQ=255)
                      tGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCg                                                                        <  1:2507759/100‑1 (MQ=255)
                      tGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCg                                                                        <  2:2069824/100‑1 (MQ=255)
                       gATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGc                                                                       <  2:57271/100‑1 (MQ=255)
                       gATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGc                                                                       >  2:1354356/1‑100 (MQ=255)
                        aTAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGcc                                                                      <  1:1010967/100‑1 (MQ=255)
                         tAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCt                                                                     >  1:1188222/1‑100 (MQ=255)
                         tAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCt                                                                     <  1:1936189/100‑1 (MQ=255)
                            aaaGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTAGATCggaagag                                                                  >  1:1648233/1‑94 (MQ=255)
                            aaaGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCCCCCAGCCTTCATGCAGTTTGATCGCCTGCt                                                                  >  1:2965594/1‑100 (MQ=255)
                                 ttCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTcc                                                             <  1:1792337/100‑1 (MQ=255)
                                 ttCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTcc                                                             >  1:1743989/1‑100 (MQ=255)
                                 ttCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTcc                                                             >  1:19636/1‑100 (MQ=255)
                                  tCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTcc                                                             <  1:842831/99‑1 (MQ=255)
                                  tCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCa                                                            >  1:1851462/1‑100 (MQ=255)
                                  tCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCa                                                            <  2:57699/100‑1 (MQ=255)
                                   cAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAg                                                           >  1:1463608/1‑100 (MQ=255)
                                   cAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAg                                                           <  2:2223281/100‑1 (MQ=255)
                                   cAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTGCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAg                                                           >  1:237901/1‑100 (MQ=255)
                                    aaTGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGt                                                          >  2:917817/1‑100 (MQ=255)
                                    aaTGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGt                                                          >  1:1037168/1‑100 (MQ=255)
                                      tGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTc                                                        <  1:1354369/100‑1 (MQ=255)
                                      tGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTc                                                        >  2:680271/1‑100 (MQ=255)
                                        aGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTccc                                                      >  1:2237343/1‑100 (MQ=255)
                                           ggCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCa                                                   <  2:1205343/100‑1 (MQ=255)
                                               cAAGCAGAGAAAAAGTAAACGGGTCGTCCACGCCAATCACCGGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGt                                               <  2:2592448/100‑1 (MQ=255)
                                               cAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGt                                               >  2:2247970/1‑100 (MQ=255)
                                               cAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGt                                               <  2:846639/100‑1 (MQ=255)
                                               cAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGt                                               >  2:2837750/1‑100 (MQ=255)
                                                   cAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGc                                           <  2:12424/100‑1 (MQ=255)
                                                    agagAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCa                                          <  2:409822/100‑1 (MQ=255)
                                                      agaAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGTAGTTTGATCGCCTGCTTTTCCACTTCCCTCACGGTATGCaca                                        <  1:2054102/100‑3 (MQ=255)
                                                      agaAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGa                                        <  1:2310294/100‑1 (MQ=255)
                                                        aaaaaGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAAc                                      <  1:229135/100‑1 (MQ=255)
                                                        aaaaaGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAAc                                      <  1:2010601/100‑1 (MQ=255)
                                                        aaaaaGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAAc                                      <  2:953956/100‑1 (MQ=255)
                                                          aaaGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACtt                                    >  2:1103171/1‑100 (MQ=255)
                                                           aaGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTc                                   <  1:2003079/100‑1 (MQ=255)
                                                            aGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTcc                                  >  1:2586240/1‑100 (MQ=255)
                                                              gAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCg                                >  1:1820822/1‑100 (MQ=255)
                                                              gAAACGTGTCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCg                                >  1:1048903/1‑100 (MQ=255)
                                                                  cGTGTCGTCCACGCCAATCACCAGCTAGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCt                            >  1:1850864/1‑100 (MQ=255)
                                                                   gtgtCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCtt                           <  2:1100506/100‑1 (MQ=255)
                                                                   gtgtCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCtt                           <  2:2555086/100‑1 (MQ=255)
                                                                    tgtCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCttt                          <  2:2066948/100‑1 (MQ=255)
                                                                    tgtCGTCCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCttt                          >  1:368756/1‑100 (MQ=255)
                                                                     gtcgtcCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTtc                         <  2:1463596/100‑1 (MQ=255)
                                                                     gtcgtcCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGATCCCGCACGGTATGCAGAACTTCCCGCCCTTtc                         <  2:1820807/100‑1 (MQ=255)
                                                                        gtcCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTctct                      <  2:2433921/100‑1 (MQ=255)
                                                                        gtcCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTctct                      >  2:45447/1‑100 (MQ=255)
                                                                        gtcCACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTctct                      <  2:1780709/100‑1 (MQ=255)
                                                                          ccACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCtaa                    >  2:97104/1‑100 (MQ=255)
                                                                          ccACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCtaa                    >  1:2142651/1‑100 (MQ=255)
                                                                          ccACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCtaa                    >  2:2484767/1‑100 (MQ=255)
                                                                           cACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCtaat                   >  2:1675313/1‑100 (MQ=255)
                                                                           cACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCtaat                   >  1:403569/1‑100 (MQ=255)
                                                                           cACGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCtaat                   <  2:1807986/100‑1 (MQ=255)
                                                                             cGCCAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCtaataa                 >  1:868187/1‑100 (MQ=255)
                                                                               cgatctaCCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACa               <  2:2461576/94‑1 (MQ=255)
                                                                               ccAATCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACa               <  1:1464917/100‑1 (MQ=255)
                                                                                   tCACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACAtttt           >  1:2373520/1‑100 (MQ=255)
                                                                                    cACCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACAttttt          >  2:2351614/1‑100 (MQ=255)
                                                                                     aCCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACaagatcg         >  1:2461598/1‑94 (MQ=255)
                                                                                     aCCAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTc         >  1:2830816/1‑100 (MQ=255)
                                                                                      ccAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTcc        >  2:1505876/1‑100 (MQ=255)
                                                                                      ccAGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTcc        >  1:465241/1‑100 (MQ=255)
                                                                                        aGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCgg      >  2:2367140/1‑100 (MQ=255)
                                                                                        aGCTCGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCgg      <  1:1157985/100‑1 (MQ=255)
                                                                                            cGTTCTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCg  >  2:63988/1‑100 (MQ=255)
                                                                                                |                                                                                               
CAGGCGCGTCACGCTATGATGTTGATAAAAAGCTTCAATGAGTGGCGCAAGCAGAGAAAAAGGAAACGTGTCGTCCACGCCAATCACCAGCTCGTTTTCCCAGCCTTCATGCAGTTTGATCGCCTGCTTTTCCAGTTCCCGCACGGTATGCAGAACTTCCCGCCCTTTCTCTAATAACATTTTTCCGGTGCG  >  NC_000913/2087887‑2088078

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: