Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,285,579 A→G T402A (ACT→GCT)  yejM → essential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,285,5790AG98.4% 420.8 / ‑3.2 122T402A (ACT→GCT) yejMessential inner membrane DUF3413 domain‑containing protein; lipid A production and membrane permeability factor
Reads supporting (aligned to +/- strand):  ref base A (0/2);  new base G (67/53);  total (67/55)
Fisher's exact test for biased strand distribution p-value = 2.01e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01

Only 100 of 122 total aligned reads displayed.

CAATCCGACGAGCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCAC  >  NC_000913/2285483‑2285675
                                                                                                |                                                                                                
cAATCCGACGAGCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTa                                                                                               >  2:1013190/1‑100 (MQ=255)
 aaTCCGACGAGCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTaa                                                                                              >  1:1946953/1‑100 (MQ=255)
   tCCGACGAGCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAAc                                                                                             >  1:218507/1‑99 (MQ=255)
    ccGACGAGCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACAt                                                                                           >  2:1036135/1‑100 (MQ=255)
    ccGACGAGCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACAt                                                                                           >  1:388887/1‑100 (MQ=255)
     cgacgaGAAGACCGCCAAGCAGTGGATACACTGGCTGCGCCGCTACGCACAAGAAGATAACTGCTGGTTCTCGTGGGTTTCTTTCAATGATGCTaat                                                                                             <  2:557340/97‑2 (MQ=255)
      gacgaGCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTg                                                                                         <  2:2651942/100‑1 (MQ=255)
      gacgaGCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTg                                                                                         >  2:1406726/1‑100 (MQ=255)
      cgatctcAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTg                                                                                         <  2:1797510/94‑1 (MQ=255)
        cgaGCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTgac                                                                                       <  1:2338038/100‑1 (MQ=255)
        cgaGCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTgac                                                                                       <  2:1443421/100‑1 (MQ=255)
        cgaGCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTga                                                                                        <  2:1953660/99‑1 (MQ=255)
           gCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTgacgac                                                                                    <  1:2712132/100‑1 (MQ=255)
           gCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTgacgac                                                                                    <  2:1985205/100‑1 (MQ=255)
             aGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAg                                                                                  <  2:2479027/100‑1 (MQ=255)
              gACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGc                                                                                 >  1:1493094/1‑100 (MQ=255)
              gACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGc                                                                                 <  1:58038/100‑1 (MQ=255)
              gACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGc                                                                                 >  1:3026512/1‑100 (MQ=255)
              gACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGc                                                                                 >  2:2005235/1‑100 (MQ=255)
                ccGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCaa                                                                               >  1:2941193/1‑100 (MQ=255)
                ccGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCaa                                                                               >  2:682546/1‑100 (MQ=255)
                ccGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCaa                                                                               >  1:1334416/1‑100 (MQ=255)
                ccGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCaa                                                                               >  1:2369795/1‑100 (MQ=255)
                  gCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATc                                                                             >  2:2136196/1‑100 (MQ=255)
                  gCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATc                                                                             >  1:1206798/1‑100 (MQ=255)
                  gCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATc                                                                             <  1:742729/100‑1 (MQ=255)
                  gCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATc                                                                             <  1:1668446/100‑1 (MQ=255)
                  gCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTACTAACATTGACGACAGCAATc                                                                             <  1:2091868/100‑1 (MQ=255)
                   ccACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGGAAt                                                                              >  1:2440362/1‑98 (MQ=255)
                     aCGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATcagc                                                                          <  1:982450/100‑1 (MQ=255)
                      cGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATcagca                                                                         <  2:1904978/100‑1 (MQ=255)
                      cGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATcagca                                                                         <  1:1290607/100‑1 (MQ=255)
                       gCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATcagcag                                                                        >  1:1807348/1‑100 (MQ=255)
                        cAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAgg                                                                       >  1:2006307/1‑100 (MQ=255)
                        cAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAgg                                                                       >  2:2838562/1‑100 (MQ=255)
                        cAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAgg                                                                       <  1:1163785/100‑1 (MQ=255)
                           tGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCAt                                                                    >  2:1505060/1‑100 (MQ=255)
                            ggATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCAtt                                                                   <  1:1579237/100‑1 (MQ=255)
                             gATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCAttt                                                                  >  1:1438546/1‑100 (MQ=255)
                             gATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCAttt                                                                  <  2:1472951/100‑1 (MQ=255)
                              aTCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGAAGCCAGCAATCAGCCGGCCttt                                                                  >  1:1007287/1‑99 (MQ=255)
                              aTCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAACGGTGCTAACATTGCCGACAGCAATCAGCAGGCATTTg                                                                 >  2:2557876/1‑100 (MQ=255)
                                cAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAAAATTGACGACAGCAATCAGCAGGCATTTg                                                                 >  1:1265922/1‑98 (MQ=255)
                                 aaCTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCa                                                               >  2:1525438/1‑99 (MQ=255)
                                 aaCTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCAc                                                              >  2:2524905/1‑100 (MQ=255)
                                     ggctggGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGaa                                                          >  2:1637627/1‑100 (MQ=255)
                                     ggctggGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGaa                                                          >  2:1251243/1‑100 (MQ=255)
                                     ggctggGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGaa                                                          >  2:1304467/1‑100 (MQ=255)
                                      gctggGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGaaa                                                         <  1:1066942/100‑1 (MQ=255)
                                         gggACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAatat                                                      >  2:803697/1‑100 (MQ=255)
                                         gggACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAatat                                                      <  2:70532/100‑1 (MQ=255)
                                          ggACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAAtata                                                     <  1:530986/100‑1 (MQ=255)
                                          ggACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAAtata                                                     <  2:1233247/100‑1 (MQ=255)
                                          ggACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAAtata                                                     <  2:1291285/100‑1 (MQ=255)
                                            aCGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGc                                                   <  1:2933963/100‑1 (MQ=255)
                                            aCGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGc                                                   <  1:1410643/100‑1 (MQ=255)
                                               cTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCgg                                                >  1:2068882/1‑100 (MQ=255)
                                                  cGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGggcg                                             >  2:485529/1‑100 (MQ=255)
                                                     acaAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCggca                                          <  2:437609/100‑1 (MQ=255)
                                                     acaAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCggca                                          <  1:2698870/100‑1 (MQ=255)
                                                     acaAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCggca                                          <  1:2244412/100‑1 (MQ=255)
                                                      caAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCggcag                                         >  1:1784766/1‑100 (MQ=255)
                                                      caAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCggcag                                         >  2:2602340/1‑100 (MQ=255)
                                                        agaagaTAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCggcaggc                                       <  2:931824/100‑1 (MQ=255)
                                                           agaTAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAAt                                    >  1:1356744/1‑100 (MQ=255)
                                                            gaTAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATg                                   >  2:592425/1‑100 (MQ=255)
                                                              tAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTc                                 >  2:2960376/1‑100 (MQ=255)
                                                              tAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTc                                 <  2:2317661/100‑1 (MQ=255)
                                                              tAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTc                                 >  2:357746/1‑100 (MQ=255)
                                                              tAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTc                                 >  2:847312/1‑100 (MQ=255)
                                                                aCCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGa                               <  2:2136737/100‑1 (MQ=255)
                                                                aCCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGa                               >  1:2746652/1‑100 (MQ=255)
                                                                aCCGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGa                               >  1:1954357/1‑100 (MQ=255)
                                                                  cGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATg                             <  1:1115581/100‑1 (MQ=255)
                                                                  cGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATg                             >  2:329692/1‑100 (MQ=255)
                                                                  cGCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATg                             <  2:530831/100‑1 (MQ=255)
                                                                   gCTGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGa                            >  1:1455163/1‑100 (MQ=255)
                                                                     tGGTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATATCCGGGCGGCAGGCAATGTCGATGAcc                          <  1:1851088/100‑1 (MQ=255)
                                                                      ggTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCa                         >  2:2851895/1‑100 (MQ=255)
                                                                      ggTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCa                         <  1:1637641/100‑1 (MQ=255)
                                                                       gTTCTCGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAg                        <  2:1814671/100‑1 (MQ=255)
                                                                         tctcGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGAt                      >  1:2756495/1‑100 (MQ=255)
                                                                          ctcGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCCGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATc                     >  1:788229/1‑100 (MQ=255)
                                                                            cGTGGGTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCaa                   >  2:1945778/1‑100 (MQ=255)
                                                                                 gTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACcgcg              <  1:1050867/100‑1 (MQ=255)
                                                                                 gTTTCTTTCAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACcgcg              >  2:2372092/1‑100 (MQ=255)
                                                                                  tttctttcAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGt             >  2:779758/1‑100 (MQ=255)
                                                                                  tttctttcAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGt             >  2:2021780/1‑100 (MQ=255)
                                                                                  tttctttcAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGt             >  1:852589/1‑100 (MQ=255)
                                                                                   ttctttcAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTg            >  2:2919141/1‑100 (MQ=255)
                                                                                   ttctttcAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTg            >  1:2694729/1‑100 (MQ=255)
                                                                                    tctttcAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGc           <  1:2524928/100‑1 (MQ=255)
                                                                                        tcAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCaa       <  2:2941167/100‑1 (MQ=255)
                                                                                        tcAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCaa       <  1:1406738/100‑1 (MQ=255)
                                                                                         cAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAAt      >  2:449249/1‑100 (MQ=255)
                                                                                         cAATGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAAt      >  2:2747853/1‑100 (MQ=255)
                                                                                           aTGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGc    <  2:307251/100‑1 (MQ=255)
                                                                                           aTGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGc    <  1:628075/100‑1 (MQ=255)
                                                                                            tGGTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCa   >  2:2806166/1‑100 (MQ=255)
                                                                                             ggTGCTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCAc  >  2:1494803/1‑100 (MQ=255)
                                                                                                |                                                                                                
CAATCCGACGAGCAGACCGCCACGCAGTGGATCAACTGGCTGGGACGCTACGCACAAGAAGATAACCGCTGGTTCTCGTGGGTTTCTTTCAATGGTACTAACATTGACGACAGCAATCAGCAGGCATTTGCACGGAAATATAGCCGGGCGGCAGGCAATGTCGATGACCAGATCAACCGCGTGCTCAATGCAC  >  NC_000913/2285483‑2285675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: