Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,372,870 T→C V105A (GTG→GCG)  arnE → undecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,372,8700TC96.7% 303.3 / ‑0.8 91V105A (GTG→GCG) arnEundecaprenyl phosphate‑alpha‑L‑ara4N exporter; flippase ArnEF subunit
Reads supporting (aligned to +/- strand):  ref base T (1/2);  new base C (53/35);  total (54/37)
Fisher's exact test for biased strand distribution p-value = 5.64e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

AATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGA  >  NC_000913/2372773‑2372964
                                                                                                 |                                                                                              
aaTTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGa                                                                                              <  1:948163/100‑1 (MQ=255)
aaTTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGa                                                                                              <  1:609965/100‑1 (MQ=255)
 aTTTTGTCTGGGTGACCCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGAt                                                                                             >  2:2962420/1‑100 (MQ=255)
  ttttGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATc                                                                                            <  1:256782/100‑1 (MQ=255)
    ttGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCt                                                                                          >  2:888311/1‑100 (MQ=255)
        cTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCggg                                                                                      <  2:1857154/100‑1 (MQ=255)
          gggTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAg                                                                                    >  2:2955054/1‑100 (MQ=255)
          gggTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGCTCATTATTGGCGGCATTGCGATCCTCGGGAg                                                                                    >  2:955303/1‑100 (MQ=255)
            gTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTa                                                                                  >  2:2446125/1‑100 (MQ=255)
            gTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTa                                                                                  >  1:1761501/1‑100 (MQ=255)
              gACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACg                                                                                >  2:2472267/1‑100 (MQ=255)
               aCGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACgg                                                                               >  2:1871090/1‑100 (MQ=255)
                  ccgatctcAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGgtgt                                                                            <  1:1166239/93‑1 (MQ=255)
                    ggctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTaa                                                                          >  1:1835860/1‑100 (MQ=255)
                      ctgCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATg                                                                        <  1:2093077/100‑1 (MQ=255)
                        gCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATggg                                                                      >  1:3021302/1‑100 (MQ=255)
                        gCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATggg                                                                      <  1:2592982/100‑1 (MQ=255)
                         cAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTagatcgg                                                                     >  2:1166228/1‑94 (MQ=255)
                         cAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGc                                                                     >  1:218161/1‑100 (MQ=255)
                         cAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGc                                                                     >  1:1587752/1‑100 (MQ=255)
                         cAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGc                                                                     <  2:1848277/100‑1 (MQ=255)
                         cAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGc                                                                     >  1:2749681/1‑100 (MQ=255)
                                 cTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGAtgtg                                                             <  1:862024/100‑1 (MQ=255)
                                  tgtgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTgg                                                            >  2:1319346/1‑100 (MQ=255)
                                  tgtgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTgg                                                            >  2:609721/1‑100 (MQ=255)
                                   gtgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGAtgtgag                                                           >  2:862016/1‑98 (MQ=255)
                                   gtgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTggg                                                           >  1:1623986/1‑100 (MQ=255)
                                   gtgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTggg                                                           >  1:1697406/1‑100 (MQ=255)
                                    tgGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTgggg                                                          <  2:885867/100‑1 (MQ=255)
                                     ggCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGa                                                         >  2:2933846/1‑100 (MQ=255)
                                     ggCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGa                                                         >  1:334828/1‑100 (MQ=255)
                                     ggCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGa                                                         <  2:1598584/100‑1 (MQ=255)
                                        aCGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGatta                                                      <  1:1848285/100‑1 (MQ=255)
                                           aaCCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTc                                                   >  2:352835/1‑100 (MQ=255)
                                           aaCCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGAGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTc                                                   >  2:1684799/1‑100 (MQ=255)
                                           aaCCGGTAGCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTc                                                   <  2:884106/100‑1 (MQ=255)
                                            aCCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCa                                                  >  1:2177115/1‑100 (MQ=255)
                                             ccGGTATCGCCGCGTCGCTGGTTTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAg                                                 >  2:445263/1‑100 (MQ=255)
                                             ccGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAg                                                 <  1:2810726/100‑1 (MQ=255)
                                              cGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGc                                                <  1:888319/100‑1 (MQ=255)
                                               ggTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCg                                               <  2:1835844/100‑1 (MQ=255)
                                               ggTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCg                                               >  1:354265/1‑100 (MQ=255)
                                                  aTCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTcn                                            <  2:222346/100‑2 (MQ=255)
                                                  aTCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCa                                            >  1:2869384/1‑100 (MQ=255)
                                                   ncgccgcGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGCAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCat                                           >  2:1940698/2‑100 (MQ=255)
                                                    cgccgcGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCatt                                          >  2:1671356/1‑100 (MQ=255)
                                                    cgccgcGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCatt                                          <  1:612335/100‑1 (MQ=255)
                                                    cgccgcGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCan                                           <  2:2419963/99‑2 (MQ=255)
                                                        gcgTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTg                                      <  2:2901424/100‑1 (MQ=255)
                                                         cgTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGc                                     <  1:2195429/100‑1 (MQ=255)
                                                           tCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGcc                                    <  2:1363915/99‑1 (MQ=255)
                                                            cACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTc                                  >  1:1744961/1‑100 (MQ=255)
                                                             aCTGGTGCGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTGGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCtga                                 >  2:2431007/1‑98 (MQ=255)
                                                              tccgatctgggTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAg                                <  1:2392963/92‑1 (MQ=255)
                                                              cTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAg                                >  1:2900628/1‑100 (MQ=255)
                                                                ggTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGtt                              >  2:1240181/1‑100 (MQ=255)
                                                                ggTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGtt                              >  2:2758611/1‑100 (MQ=255)
                                                                ggTGTGGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCATCAGtt                              >  2:1805562/1‑100 (MQ=255)
                                                                 gtgtGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTg                             <  1:1319359/100‑1 (MQ=255)
                                                                  tgtgGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTgc                            >  1:1775072/1‑100 (MQ=255)
                                                                   gtgGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTgcg                           <  1:308913/100‑1 (MQ=255)
                                                                   gtgGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTgcg                           <  1:903916/100‑1 (MQ=255)
                                                                    tgGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTgcgc                          >  2:381291/1‑100 (MQ=255)
                                                                    tgGGGTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTgcgc                          <  1:2734907/100‑1 (MQ=255)
                                                                     ggggTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCa                         <  2:1078010/100‑1 (MQ=255)
                                                                     ggggTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCa                         <  1:629152/100‑1 (MQ=255)
                                                                      gggTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAgagatcgga                        >  2:2392941/1‑92 (MQ=255)
                                                                      gggTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCaa                        >  2:2378678/1‑100 (MQ=255)
                                                                      gggTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCaa                        >  2:1792976/1‑100 (MQ=255)
                                                                        ntGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAAtt                      >  2:330462/2‑100 (MQ=255)
                                                                        gTGGCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAAtt                      >  2:214985/1‑100 (MQ=255)
                                                                           gCGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAg                   <  2:189400/100‑1 (MQ=255)
                                                                            cGTTCATTATTGGCGGCATTGCGATCCTCGGGAGTACGTTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTAGTGCCTCAGTTGCGCAATTAAGc                  <  2:1761486/100‑1 (MQ=255)
                                                                               tCATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTg               <  2:302266/100‑1 (MQ=255)
                                                                                cATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTgg              >  1:814112/1‑100 (MQ=255)
                                                                                cATTATTGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTgg              >  1:250959/1‑100 (MQ=255)
                                                                                 attattGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTggg             >  1:1147914/1‑100 (MQ=255)
                                                                                 attattGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTggg             <  1:2347651/100‑1 (MQ=255)
                                                                                  ttattGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGt            >  1:671455/1‑100 (MQ=255)
                                                                                   tattGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGGTGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGtt           >  1:216757/1‑100 (MQ=255)
                                                                                    attGGCGGCATTGCGATCCTCGGGAGTACGGGGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGttt          >  1:2376614/1‑100 (MQ=255)
                                                                                      tGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTg        >  1:2062799/1‑100 (MQ=255)
                                                                                      tGGCGGCATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTg        >  1:2065276/1‑100 (MQ=255)
                                                                                       ggcggcATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGCTTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgc       >  2:1658999/1‑100 (MQ=255)
                                                                                       ggcggcATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgc       <  2:1110316/100‑1 (MQ=255)
                                                                                       ggcggcATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgc       >  1:978983/1‑100 (MQ=255)
                                                                                         cggcATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgcgg     >  2:2379536/1‑100 (MQ=255)
                                                                                         cggcATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgcgg     <  2:218158/100‑1 (MQ=255)
                                                                                          ggcATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgcggc    <  1:1543124/100‑1 (MQ=255)
                                                                                           gcATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTgcggcg   <  1:2962446/100‑1 (MQ=255)
                                                                                            cATTGCGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGa  >  2:1574734/1‑100 (MQ=255)
                                                                                                 |                                                                                              
AATTTTGTCTGGGTGACGCTGGCTGCAGTAAAACTGTGGCACGAACCGGTATCGCCGCGTCACTGGTGTGGGGTGGCGTTCATTATTGGCGGCATTGTGATCCTCGGGAGTACGGTGTAATGGGCCTGATGTGGGGATTATTCAGCGTCATTATTGCCTCAGTTGCGCAATTAAGCCTGGGTTTTGCGGCGA  >  NC_000913/2372773‑2372964

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: