Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,455,793 G→A intergenic (‑147/+534) yfcV ← / ← sixA putative fimbrial‑like adhesin protein/phosphohistidine phosphatase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,455,7930GA99.3% 498.7 / ‑4.7 145intergenic (‑147/+534)yfcV/sixAputative fimbrial‑like adhesin protein/phosphohistidine phosphatase
Reads supporting (aligned to +/- strand):  ref base G (1/0);  new base A (77/67);  total (78/67)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.37e-01

Only 100 of 145 total aligned reads displayed.

GATTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATCTTAAG  >  NC_000913/2455697‑2455889
                                                                                                |                                                                                                
 aTTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATa                                                                                              >  1:2713031/1‑100 (MQ=255)
  ttCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAg                                                                                             >  1:2659570/1‑100 (MQ=255)
   tCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGt                                                                                            <  1:1182896/100‑1 (MQ=255)
    cAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTg                                                                                           >  1:2173755/1‑100 (MQ=255)
    cAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTg                                                                                           <  1:1961292/100‑1 (MQ=255)
    cAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTg                                                                                           >  1:2777602/1‑100 (MQ=255)
     aGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCGAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGACAAATAGTGa                                                                                          >  2:1081772/1‑100 (MQ=255)
       tCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATg                                                                                        >  2:1606837/1‑100 (MQ=255)
        cAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGt                                                                                       <  1:1606851/100‑1 (MQ=255)
        cAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGt                                                                                       >  1:1531094/1‑100 (MQ=255)
        cAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGt                                                                                       >  2:591090/1‑100 (MQ=255)
         aaTAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGt                                                                                       <  2:1205830/99‑1 (MQ=255)
           tAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGtttt                                                                                    <  2:2847510/100‑1 (MQ=255)
            aGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTc                                                                                   <  1:1723388/100‑1 (MQ=255)
             gCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCa                                                                                  >  1:315964/1‑100 (MQ=255)
              ccAATGTTTATACCCAGTCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCAc                                                                                 <  2:2684098/100‑1 (MQ=255)
              ccAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCAc                                                                                 <  2:963575/100‑1 (MQ=255)
              ccAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCAc                                                                                 <  2:2122449/100‑1 (MQ=255)
               cAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACt                                                                                >  1:462619/1‑100 (MQ=255)
                  tGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTccc                                                                             >  1:883396/1‑100 (MQ=255)
                   gTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTcccc                                                                            <  2:33517/100‑1 (MQ=255)
                   gTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTcccc                                                                            <  2:1296971/100‑1 (MQ=255)
                   gTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTcccc                                                                            <  1:3005490/100‑1 (MQ=255)
                     ttATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTg                                                                          <  2:1434912/100‑1 (MQ=255)
                      tataCCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGAGTCAAATAGTGATGTTTTCACTCCCCTGa                                                                         >  2:1046276/1‑100 (MQ=255)
                       ataCCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGAt                                                                        <  2:2114841/100‑1 (MQ=255)
                       ataCCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGAt                                                                        <  1:2830094/100‑1 (MQ=255)
                        taCCCAGCCATTGAATACGGCATGCGATCCAAAAAAACATGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGAtt                                                                       <  2:126191/100‑1 (MQ=255)
                         aCCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTc                                                                      <  2:63132/100‑1 (MQ=255)
                         aCCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTc                                                                      <  1:2871453/100‑1 (MQ=255)
                          cccAGCCATTGGATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCa                                                                     >  1:2063255/1‑100 (MQ=255)
                          cccAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCa                                                                     >  2:205867/1‑100 (MQ=255)
                          cccAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCa                                                                     >  1:824956/1‑100 (MQ=255)
                           ccAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCaa                                                                    >  2:241708/1‑100 (MQ=255)
                           ccAGCCATTGAATACGGAATGCGATCCAAAAAAACAGGACGTAAAAACTGTATTAGATGGTGTGGGGACAAATAGTGATGTTTTCACTACCCTGATTCa                                                                     >  2:2263450/1‑99 (MQ=255)
                            caaccATTGAATACATCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCaa                                                                    <  2:1692989/96‑1 (MQ=255)
                            cAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGACAAATAGTGATGTTTTCACTCCCCTGATTCaat                                                                   >  2:2962874/1‑100 (MQ=255)
                                cATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCTGATTCaatttaa                                                               >  1:906881/1‑100 (MQ=255)
                                 aTTGAATACGGCATGCGATCCATAAAAACAGCTCGTATAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCaatttaat                                                              <  2:2042920/100‑1 (MQ=255)
                                 aTTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCaatttaat                                                              <  1:1931591/100‑1 (MQ=255)
                                 aTTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCaatttaat                                                              >  2:2660984/1‑100 (MQ=255)
                                    gAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAAtttt                                                           >  1:1288152/1‑100 (MQ=255)
                                      aTACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTAc                                                         <  1:937770/100‑1 (MQ=255)
                                      aTACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTGTTCACTCCCCTGATTCAATTTAATTTTAc                                                         <  2:448459/100‑1 (MQ=255)
                                        aCGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTa                                                       <  1:2158429/100‑1 (MQ=255)
                                         cGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTaa                                                      >  1:1197895/1‑100 (MQ=255)
                                           gCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATg                                                    <  1:2268480/100‑1 (MQ=255)
                                              tGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGt                                                 <  1:591097/100‑1 (MQ=255)
                                              tGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGt                                                 <  2:498216/100‑1 (MQ=255)
                                                 gATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTaaa                                              <  1:1806189/100‑1 (MQ=255)
                                                  aTCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAAt                                             <  2:2713007/100‑1 (MQ=255)
                                                   tCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATg                                            >  1:3002233/1‑100 (MQ=255)
                                                    ccAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGt                                           >  1:1515518/1‑100 (MQ=255)
                                                    ccAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAAATTTACTCATTAGTaaaatt                                           <  1:1706013/100‑4 (MQ=255)
                                                     cAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTa                                          >  1:1818675/1‑100 (MQ=255)
                                                     cAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTa                                          >  1:2916219/1‑100 (MQ=255)
                                                     cAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGAGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTa                                          >  2:1864358/1‑100 (MQ=255)
                                                      aaaaaaaCAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAg                                         >  1:1137675/1‑100 (MQ=255)
                                                      aaaaaaaCAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAg                                         >  1:1016636/1‑100 (MQ=255)
                                                       aaaaaaCAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGa                                        >  1:2349526/1‑100 (MQ=255)
                                                       aaaaaaCAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGa                                        <  2:166867/100‑1 (MQ=255)
                                                         aaaaCAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGAtt                                      <  1:817739/100‑1 (MQ=255)
                                                          aaaCAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGAttt                                     <  2:2777578/100‑1 (MQ=255)
                                                          aaaCAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGAtt                                      <  1:1359542/99‑1 (MQ=255)
                                                             cAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATttaat                                  >  1:323351/1‑100 (MQ=255)
                                                              aGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATttaatt                                 <  1:1369426/100‑1 (MQ=255)
                                                              aGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATttaatt                                 <  1:2676574/100‑1 (MQ=255)
                                                              aGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATGCAATTTAATTTTACTAATGAGTAACTGTAGATttaatt                                 <  2:1874565/100‑1 (MQ=255)
                                                              aGGTCGTAAAAAATGTATTCGTTGGTGTGGAGTCAAATCGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATttaat                                  <  2:1046216/99‑1 (MQ=255)
                                                               ggTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATttaatta                                >  2:2483713/1‑100 (MQ=255)
                                                                gTCGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATttaattaa                               <  2:843644/100‑1 (MQ=255)
                                                                  cGTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAata                             <  1:2029344/100‑1 (MQ=255)
                                                                   gTAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAatat                            >  2:2981051/1‑100 (MQ=255)
                                                                    tAAAAAATGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTATCTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAatat                            >  2:1974358/1‑99 (MQ=255)
                                                                     aaaaaaTGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAatatat                          >  2:1010673/1‑100 (MQ=255)
                                                                      aaaaaTGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATAtt                         <  2:96131/100‑1 (MQ=255)
                                                                        aaaTGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGa                       >  1:2123383/1‑100 (MQ=255)
                                                                          aTGTATTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCATTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATa                     >  2:771651/1‑100 (MQ=255)
                                                                              aTTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAgggg                 >  2:279714/1‑100 (MQ=255)
                                                                              aTTCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAggg                  >  2:1295255/1‑99 (MQ=255)
                                                                               ttCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGa                >  1:983265/1‑100 (MQ=255)
                                                                                tCGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGaa               >  2:2369119/1‑100 (MQ=255)
                                                                                 cGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGaaa              >  2:1407381/1‑100 (MQ=255)
                                                                                 cGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGaaa              >  2:1914831/1‑100 (MQ=255)
                                                                                 cGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGaaa              >  2:954023/1‑100 (MQ=255)
                                                                                 cGTTGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGaaa              >  2:225835/1‑100 (MQ=255)
                                                                                   ttGGTGTGGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGa            >  1:409582/1‑100 (MQ=255)
                                                                                       tgtgGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTAt        >  2:45659/1‑100 (MQ=255)
                                                                                        gtgGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATc       <  1:549277/100‑1 (MQ=255)
                                                                                        gtgGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATc       <  2:2145713/100‑1 (MQ=255)
                                                                                         tgGGGTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATCt      <  1:2483735/100‑1 (MQ=255)
                                                                                          ggggTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATCtt     >  2:2702474/1‑100 (MQ=255)
                                                                                          ggggTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATCtt     >  2:788318/1‑100 (MQ=255)
                                                                                           gggTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATCTTa    <  2:1515505/100‑1 (MQ=255)
                                                                                           gggTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATCTTa    <  1:1859926/100‑1 (MQ=255)
                                                                                           gggTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATCTTa    >  2:368447/1‑100 (MQ=255)
                                                                                           gggTCAAATAGTGATGTTTTCACTCCCCTCATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATCTTa    <  2:1825603/100‑1 (MQ=255)
                                                                                             gTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATCTTaa   >  2:1577294/1‑99 (MQ=255)
                                                                                             gTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATCTTAAg  >  2:14604/1‑100 (MQ=255)
                                                                                             gTCAAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATCTTAAg  >  2:2560317/1‑100 (MQ=255)
                                                                                                |                                                                                                
GATTCAGTCAATAGCCAATGTTTATACCCAGCCATTGAATACGGCATGCGATCCAAAAAAACAGGTCGTAAAAAATGTATTCGTTGGTGTGGGGTCGAATAGTGATGTTTTCACTCCCCTGATTCAATTTAATTTTACTAATGAGTAAATGTAGATTTAATTAATATATTGATAGGGGAAAGATTATCTTAAG  >  NC_000913/2455697‑2455889

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: