| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 2,731,312 | G→A | intergenic (‑155/+288) | rrsG ← / ← clpB | 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 2,731,312 | 0 | G | A | 95.9% | 151.6 / ‑3.5 | 49 | intergenic (‑155/+288) | rrsG/clpB | 16S ribosomal RNA of rrnG operon/protein disaggregation chaperone |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (22/25); minor base T (1/1); total (23/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
AGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGA > NC_000913/2731274‑2731404 | aGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTc < 1:2459183/100‑1 (MQ=11) gATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCt < 2:1585388/100‑1 (MQ=14) aTTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCtt < 2:1461576/100‑1 (MQ=17) ttGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTTACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCttt < 1:454975/100‑1 (MQ=12) tGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg < 1:1411612/100‑1 (MQ=18) tGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg > 1:72859/1‑100 (MQ=25) cTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt > 1:1859030/1‑100 (MQ=255) cTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt > 1:2609437/1‑100 (MQ=38) gATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt > 2:165673/1‑100 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc < 1:1171729/100‑1 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc < 2:1086445/100‑1 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc > 1:86812/1‑100 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc < 1:431460/100‑1 (MQ=255) aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc < 1:2606996/100‑1 (MQ=255) aTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTg < 2:933926/100‑1 (MQ=255) ttGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGt < 2:1588163/100‑1 (MQ=255) ttGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGt < 2:86811/100‑1 (MQ=255) ttGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGt > 2:1368144/1‑100 (MQ=255) ttGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGt > 2:873974/1‑100 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg < 2:570676/100‑1 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg > 1:2849998/1‑100 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg < 2:630169/100‑1 (MQ=255) gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg < 1:2240506/100‑1 (MQ=255) ttAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga > 2:1977089/1‑100 (MQ=255) tAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgag > 1:2672597/1‑100 (MQ=255) tAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgag < 2:2831271/100‑1 (MQ=255) aaaGAGCAGTGCCGCTTCTCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga > 1:2550258/1‑100 (MQ=255) aaaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga > 1:1533884/1‑100 (MQ=255) aaaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga < 1:1546519/100‑1 (MQ=255) aaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAt > 1:1694603/1‑100 (MQ=255) aaGAGCAGTACCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAt < 2:373938/100‑1 (MQ=255) agagCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAtt > 2:1457445/1‑100 (MQ=255) agagCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAtt > 1:640792/1‑100 (MQ=255) gagCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtc > 1:857107/1‑100 (MQ=255) gagCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtc > 1:611682/1‑100 (MQ=255) gagCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtc < 1:1929310/100‑1 (MQ=255) gCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc < 1:2332546/100‑1 (MQ=255) cAGTGCCGCTTCACTTTTTCTTATTTTCTCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa > 2:2673437/1‑100 (MQ=18) cAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa < 2:1477154/100‑1 (MQ=255) aGTGCCGCTTCTCTTTTTCTCGGCGGCGCGGGGTGTGCATAATACGCCTTCAAGCTACAGAGTCAAGCATTTCTTTGCGTTTCTCTGGTGAGATTCTCa < 2:909673/99‑1 (MQ=17) aGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg < 2:1929828/100‑1 (MQ=255) aGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAg < 2:1970523/100‑1 (MQ=255) gTGCCGCTTCACTCTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg < 1:2470369/100‑1 (MQ=255) tGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga > 2:751885/1‑100 (MQ=255) gCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag > 1:2221403/1‑100 (MQ=255) gCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag > 1:2319934/1‑100 (MQ=255) gCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgag > 1:2320164/1‑100 (MQ=255) ccGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgaga < 2:1543145/100‑1 (MQ=255) ccGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgaga > 1:389128/1‑100 (MQ=255) | AGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGA > NC_000913/2731274‑2731404 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |