Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,749,038 T→C Y89H (TAC→CAC)  yfjD → UPF0053 family inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,749,0380TC98.3% 403.8 / ‑2.8 116Y89H (TAC→CAC) yfjDUPF0053 family inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (2/0);  new base C (64/50);  total (66/50)
Fisher's exact test for biased strand distribution p-value = 5.05e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.62e-01

Only 100 of 116 total aligned reads displayed.

TAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTA  >  NC_000913/2748944‑2749132
                                                                                              |                                                                                              
tAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGc                                                                                           >  2:2994712/1‑100 (MQ=255)
  aGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTAAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGa                                                                                         >  2:1686871/1‑100 (MQ=255)
   gCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGAt                                                                                        >  1:1475806/1‑100 (MQ=255)
   gCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGAACTATTGTTGGGATGCGTTTGCACGGc                                                                                           >  2:1474285/1‑97 (MQ=255)
      aGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCg                                                                                     >  2:2056415/1‑100 (MQ=255)
       gACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCgg                                                                                    >  1:1287720/1‑100 (MQ=255)
        aCCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCggg                                                                                   <  2:372020/100‑1 (MQ=255)
         ccGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGc                                                                                  >  1:2126314/1‑100 (MQ=255)
         ccGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGc                                                                                  >  2:1569337/1‑100 (MQ=255)
         ccGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGc                                                                                  >  2:360354/1‑100 (MQ=255)
         ccGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGc                                                                                  >  2:2183188/1‑100 (MQ=255)
         ccGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGc                                                                                  <  1:1617609/100‑1 (MQ=255)
         ccGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGc                                                                                  >  1:654557/1‑100 (MQ=255)
            ccTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTg                                                                               <  1:2762832/100‑1 (MQ=255)
             cTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTgg                                                                              <  1:751332/100‑1 (MQ=255)
             cTGATAAGCCTGGTGTTAATCGGCAAGAACCGGGTCAATATTCTTGCCTCCGCGCTCGGCACTAATGTTGGGCTGCGTTTGCACGGCGATGCGGGCGTgg                                                                              >  1:1145779/1‑100 (MQ=255)
              tGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGc                                                                             >  1:689413/1‑100 (MQ=255)
               gATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCa                                                                            <  1:1396441/100‑1 (MQ=255)
               gATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCa                                                                            >  2:231679/1‑100 (MQ=255)
               gATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCa                                                                            >  2:230998/1‑100 (MQ=255)
                 tAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAAt                                                                          >  1:1008141/1‑100 (MQ=255)
                  aaGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTTGCAAtt                                                                         >  2:1006170/1‑100 (MQ=255)
                   aGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTg                                                                        <  1:2407245/100‑1 (MQ=255)
                   aGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTg                                                                        >  2:2953182/1‑100 (MQ=255)
                    gCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGc                                                                       >  1:1392151/1‑100 (MQ=255)
                     ccTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCg                                                                      <  2:1861277/100‑1 (MQ=255)
                     ccTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCg                                                                      <  1:2462057/100‑1 (MQ=255)
                      cTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGa                                                                     >  1:1584460/1‑100 (MQ=255)
                       tGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGTGGGCGTGGCAATTGCGAc                                                                    <  2:2799466/100‑1 (MQ=255)
                          tgtTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTgg                                                                 >  2:2819111/1‑100 (MQ=255)
                          tgtTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTAGTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTgg                                                                 <  2:2018029/100‑1 (MQ=255)
                           gtTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGgt                                                                >  2:1951036/1‑100 (MQ=255)
                             tAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGgtgt                                                              >  2:3030390/1‑100 (MQ=255)
                             tAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGgtgt                                                              >  1:1282975/1‑100 (MQ=255)
                              aaTCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTACGACTGGtgtg                                                             >  1:2843594/1‑100 (MQ=255)
                                tCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCt                                                           >  2:1864864/1‑100 (MQ=255)
                                 cGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTg                                                          >  1:26944/1‑100 (MQ=255)
                                 cGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAACTGCGACTGGTGTGCTg                                                          >  2:1770279/1‑100 (MQ=255)
                                   gCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGAc                                                        >  2:858694/1‑100 (MQ=255)
                                    cAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACt                                                       <  1:2261176/100‑1 (MQ=255)
                                       tAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACtttt                                                    >  2:2848083/1‑100 (MQ=255)
                                       tAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACtttt                                                    >  1:1106981/1‑100 (MQ=255)
                                        aaCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTg                                                   <  1:2794970/100‑1 (MQ=255)
                                         aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACttttat                                                  <  1:2054536/100‑3 (MQ=255)
                                         aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGt                                                  <  2:2395629/100‑1 (MQ=255)
                                         aCCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGt                                                  <  2:931242/100‑1 (MQ=255)
                                          ccTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTc                                                 <  2:1668464/100‑1 (MQ=255)
                                            tGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGt                                               >  2:2785816/1‑100 (MQ=255)
                                             ngTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTa                                              >  1:1493511/2‑100 (MQ=255)
                                                cAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTg                                           >  1:2718864/1‑100 (MQ=255)
                                                  atatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGt                                         >  1:927515/1‑100 (MQ=255)
                                                  atatTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGt                                         <  1:2994738/100‑1 (MQ=255)
                                                     ttCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTAtt                                      >  2:1288119/1‑100 (MQ=255)
                                                      tCTTGCCTCCGCGCTCGGAACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTAt                                       <  1:1320730/98‑1 (MQ=255)
                                                        ttGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTg                                    <  2:2768526/99‑1 (MQ=255)
                                                         tGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGc                                   <  1:29735/99‑1 (MQ=255)
                                                         tGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCt                                  >  1:869797/1‑100 (MQ=255)
                                                         tGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCt                                  >  2:2631980/1‑100 (MQ=255)
                                                         tGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCt                                  <  1:789878/100‑1 (MQ=255)
                                                         tGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCt                                  <  1:658563/100‑1 (MQ=255)
                                                          gCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTg                                 <  1:1686885/100‑1 (MQ=255)
                                                          gCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTg                                 <  2:2432501/100‑1 (MQ=255)
                                                          gCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTg                                 >  2:803175/1‑100 (MQ=255)
                                                           ccTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa                                <  2:869789/100‑1 (MQ=255)
                                                           ccTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGa                                <  1:1231337/100‑1 (MQ=255)
                                                            cTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAg                               <  2:1584446/100‑1 (MQ=255)
                                                             tCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGTGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg                              <  2:1650967/100‑1 (MQ=255)
                                                             tCCGCGCTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg                              <  2:770008/100‑1 (MQ=255)
                                                              ccGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAgg                              >  2:2597089/1‑99 (MQ=255)
                                                                gcgcTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAt                           >  1:1049520/1‑100 (MQ=255)
                                                                  gcTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTAt                           <  2:2541019/98‑1 (MQ=255)
                                                                   cTCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGc                        >  2:2535030/1‑100 (MQ=255)
                                                                    tCGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGcc                       <  1:2183207/100‑1 (MQ=255)
                                                                     cGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCg                      >  2:2069056/1‑100 (MQ=255)
                                                                     cGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCg                      <  2:521875/100‑1 (MQ=255)
                                                                     cGGCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCg                      <  2:1287707/100‑1 (MQ=255)
                                                                      ggCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGGTGAGGTATTGCCGa                     <  1:437744/100‑1 (MQ=255)
                                                                       gCACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGaa                    <  2:2735174/100‑1 (MQ=255)
                                                                        cACTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGaaa                   >  2:1063711/1‑100 (MQ=255)
                                                                          cTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAAc                 >  2:788965/1‑100 (MQ=255)
                                                                          cTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAAc                 <  1:360359/100‑1 (MQ=255)
                                                                          cTATTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAAc                 >  2:2087688/1‑100 (MQ=255)
                                                                            aTTGTTGGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCa               <  1:2002019/100‑1 (MQ=255)
                                                                              tgttgGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCAtt             <  1:606118/100‑1 (MQ=255)
                                                                              tgttgGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCAtt             <  2:496704/100‑1 (MQ=255)
                                                                               gttgGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTg            <  2:1145769/100‑1 (MQ=255)
                                                                               gttgGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTg            >  1:2636811/1‑100 (MQ=255)
                                                                                 tgGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGcc          <  1:1875703/100‑1 (MQ=255)
                                                                                 tgGGATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGcc          >  1:1312386/1‑100 (MQ=255)
                                                                                  gggATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTTTGCTGACTGTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCcg         >  2:2531184/1‑100 (MQ=255)
                                                                                  gggATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCcg         >  1:1710964/1‑100 (MQ=255)
                                                                                   ggATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCcgc        >  2:1193687/1‑100 (MQ=255)
                                                                                   ggATGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCcgc        >  2:2438652/1‑100 (MQ=255)
                                                                                     aTGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAACACCATTGCcgcac      <  1:2056433/100‑3 (MQ=255)
                                                                                      tGCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCt     >  2:2052075/1‑100 (MQ=255)
                                                                                       gCGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTg    >  2:1536096/1‑100 (MQ=255)
                                                                                        cGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGt   >  2:2433154/1‑100 (MQ=255)
                                                                                        cGTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGt   <  1:1951054/100‑1 (MQ=255)
                                                                                         gTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTa  >  2:2757322/1‑100 (MQ=255)
                                                                                         gTTTGCACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTa  >  1:2825981/1‑100 (MQ=255)
                                                                                              |                                                                                              
TAAGCCAGACCGCCTGATAAGCCTGGTGTTAATCGGCAATAACCTGGTCAATATTCTTGCCTCCGCGCTCGGCACTATTGTTGGGATGCGTTTGTACGGCGATGCGGGCGTGGCAATTGCGACTGGTGTGCTGACTTTTGTCGTACTGGTATTTGCTGAGGTATTGCCGAAAACCATTGCCGCGCTGTA  >  NC_000913/2748944‑2749132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑