BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,468,389	T→C	S489G (AGT→GGT)	chiA ←	periplasmic endochitinase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,468,389	0	T	C	97.1%	353.9 / ‑0.6	104	S489G (AGT→GGT)	chiA	periplasmic endochitinase
Reads supporting (aligned to +/- strand): ref base T (1/2); new base C (48/53); total (49/55)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01

Only 100 of 105 total aligned reads displayed.

TCTTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGTTC  >  NC_000913/3468292‑3468485

tctTCGTTGCTATAGCTCTGATCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACctt                                                                                                >  1:2725960/1‑100 (MQ=255)

 ctTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTg                                                                                               <  1:132008/100‑1 (MQ=255)

 ctTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTg                                                                                               >  2:1650641/1‑100 (MQ=255)

 ctTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTg                                                                                               >  1:2563234/1‑100 (MQ=255)

     gTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTc                                                                                           >  1:818667/1‑100 (MQ=255)

       tGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCa                                                                                         <  1:997548/100‑1 (MQ=255)

       tGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCa                                                                                         <  2:2916379/100‑1 (MQ=255)

        gCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAg                                                                                        >  1:66974/1‑100 (MQ=255)

         cTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGa                                                                                       <  2:1468699/100‑1 (MQ=255)

         cTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGa                                                                                       >  1:656557/1‑100 (MQ=255)

         cTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGa                                                                                       <  1:2750803/100‑1 (MQ=255)

         cTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGa                                                                                       >  2:1965065/1‑100 (MQ=255)

          nataGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGaa                                                                                      >  2:415401/2‑100 (MQ=255)

           ataGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAAc                                                                                     >  1:1176376/1‑100 (MQ=255)

            taGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACa                                                                                    >  2:1018497/1‑100 (MQ=255)

              gCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAgc                                                                                  <  1:422483/100‑1 (MQ=255)

              gCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAgc                                                                                  >  2:32717/1‑100 (MQ=255)

               ctctGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAgcg                                                                                 <  1:1346102/100‑1 (MQ=255)

               ctctGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGAGTTGTCACCTTGCGTCCAGAACAgcg                                                                                 <  1:1846107/100‑1 (MQ=255)

                tctGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAgcgc                                                                                <  1:203117/100‑1 (MQ=255)

                 ctGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCt                                                                               <  1:1851479/100‑1 (MQ=255)

                 ctGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCt                                                                               >  2:71013/1‑100 (MQ=255)

                 ctGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGAAGGGTTGTCACCTTGCGTCCAGAANAGCGCt                                                                               >  1:2319633/1‑100 (MQ=255)

                  tGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCtt                                                                              <  2:1691070/100‑1 (MQ=255)

                   gAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTc                                                                             <  2:2974037/100‑1 (MQ=255)

                   gAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTc                                                                             <  2:150948/100‑1 (MQ=255)

                      ccTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATa                                                                          <  1:1929780/100‑1 (MQ=255)

                      ccTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATa                                                                          <  1:1302042/100‑1 (MQ=255)

                        ttACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAg                                                                        <  2:1375789/100‑1 (MQ=255)

                         tACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGc                                                                       >  2:2014747/1‑100 (MQ=255)

                          aCCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAg                                                                        <  1:1739818/98‑1 (MQ=255)

                           ccTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCt                                                                     >  2:1301371/1‑100 (MQ=255)

                           ccTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCt                                                                     >  2:1723006/1‑100 (MQ=255)

                            cTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGTAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGccgg                                                                    <  1:2048523/100‑3 (MQ=255)

                             tAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGc                                                                   >  1:1263813/1‑100 (MQ=255)

                              aaCGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGcc                                                                  >  1:2354335/1‑100 (MQ=255)

                              aaCGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGcc                                                                  >  1:1544518/1‑100 (MQ=255)

                                cGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCa                                                                >  2:15869/1‑100 (MQ=255)

                                cGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCa                                                                <  1:870741/100‑1 (MQ=255)

                                  gCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATc                                                              >  1:1365298/1‑100 (MQ=255)

                                   cTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATca                                                             <  1:1650655/100‑1 (MQ=255)

                                   cTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATca                                                             <  2:1805764/100‑1 (MQ=255)

                                   cTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATca                                                             >  1:1362188/1‑100 (MQ=255)

                                     tCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaac                                                           <  1:2218358/100‑1 (MQ=255)

                                     tCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaa                                                            <  2:2725936/99‑1 (MQ=255)

                                      ccACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaaca                                                          <  1:468990/100‑1 (MQ=255)

                                       cACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaacaa                                                         >  2:2798560/1‑100 (MQ=255)

                                        aCGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaacaan                                                        <  1:415405/100‑2 (MQ=255)

                                        aCGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaacaac                                                        <  2:2874654/100‑1 (MQ=255)

                                        aCGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATcaacaac                                                        >  2:2835917/1‑100 (MQ=255)

                                            gcggcTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAAcg                                                    <  1:1018507/100‑1 (MQ=255)

                                               gcTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGa                                                 <  1:2411552/100‑1 (MQ=255)

                                                 tATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATc                                               <  2:1951435/100‑1 (MQ=255)

                                                  aTTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCa                                              >  2:585669/1‑100 (MQ=255)

                                                    tGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATGAGCCTGCCCATCAACAACAACGCGATCAcc                                            >  2:1770534/1‑100 (MQ=255)

                                                    tGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCAcc                                            <  2:1596971/100‑1 (MQ=255)

                                                    tGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCAcc                                            <  1:2327575/100‑1 (MQ=255)

                                                     gatACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCt                                           >  1:2811978/3‑100 (MQ=255)

                                                        aCCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTc                                        <  1:2798585/100‑1 (MQ=255)

                                                         ccGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCa                                       <  1:2355896/100‑1 (MQ=255)

                                                         ccGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCa                                       <  2:818071/100‑1 (MQ=255)

                                                            gTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGt                                    >  2:1636373/1‑100 (MQ=255)

                                                             tGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTa                                   <  1:261761/100‑1 (MQ=255)

                                                             tGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACATTTCACGTa                                   >  2:2901012/1‑100 (MQ=255)

                                                              ggCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTac                                  <  1:1732075/100‑1 (MQ=255)

                                                                cGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTacac                                >  2:2522056/1‑100 (MQ=255)

                                                                cGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTacac                                <  1:434508/100‑1 (MQ=255)

                                                                  ttttGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAg                              <  1:2656824/100‑1 (MQ=255)

                                                                    ttGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTg                            >  1:1589253/1‑100 (MQ=255)

                                                                     tGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTgg                           <  2:1949569/100‑1 (MQ=255)

                                                                     tGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTgg                           >  1:1568315/1‑100 (MQ=255)

                                                                     tGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTgg                           <  2:2306107/100‑1 (MQ=255)

                                                                     tGGTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTgg                           <  2:1365285/100‑1 (MQ=255)

                                                                      aaaacGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGa                          >  1:694898/5‑100 (MQ=255)

                                                                       gTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGAt                         >  1:320862/1‑100 (MQ=255)

                                                                       gTTCGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGAt                         >  1:2532657/1‑100 (MQ=255)

                                                                        ttCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATg                        >  1:952317/1‑100 (MQ=255)

                                                                        aacGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATg                        <  1:1591638/98‑1 (MQ=255)

                                                                          cGCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCg                      >  2:2607195/1‑100 (MQ=255)

                                                                           gCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCgg                     >  1:826021/1‑100 (MQ=255)

                                                                           gCCACCAGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCgg                     >  2:3010105/1‑100 (MQ=255)

                                                                            ccaccaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGa                    >  1:2727548/1‑100 (MQ=255)

                                                                            ccaccaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGa                    >  2:3030496/1‑100 (MQ=255)

                                                                             caccaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGAc                   >  1:2673185/1‑100 (MQ=255)

                                                                             caccaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGAc                   >  1:1364881/1‑100 (MQ=255)

                                                                             caccaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGAc                   <  1:71014/100‑1 (MQ=255)

                                                                              accaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGAcc                  >  1:810292/1‑100 (MQ=255)

                                                                              accaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGAcc                  <  2:1437157/100‑1 (MQ=255)

                                                                              accaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGAcc                  >  1:2164247/1‑100 (MQ=255)

                                                                               ccaGAGCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCa                 >  2:112148/1‑100 (MQ=255)

                                                                                    gCAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCtt            >  1:970730/1‑100 (MQ=255)

                                                                                     cAGGGTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCttt           <  2:2884998/100‑1 (MQ=255)

                                                                                       gggTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCg         <  2:656550/100‑1 (MQ=255)

                                                                                       gggTTGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCg         <  2:814724/100‑1 (MQ=255)

                                                                                        ggTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCgn        <  2:952308/100‑2 (MQ=255)

                                                                                          ttGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCg      <  1:1654709/100‑1 (MQ=255)

                                                                                           tGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCgg     <  2:1001816/100‑1 (MQ=255)

                                                                                           tGTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCgg     >  1:618821/1‑100 (MQ=255)

                                                                                            gTCACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGt    >  2:85424/1‑100 (MQ=255)

                                                                                              cACCTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGCCCATGCTTTCGCCGGTTc  >  1:2816567/1‑100 (MQ=255)

TCTTCGTTGCTATAGCTCTGAGCCTTACCTAACGGCTTCCACGGGCGGCTATTGCTACCGGTGGCGTTTTGGTTCGCCACCAGAGCAGGGTTGTCACTTTGCGTCCAGAACAGCGCTTCATAAGCCTGCCCATCAACAACAACGCGATCACCTTTCACGTACACAGTGGATGCGGACCATGCTTTCGCCGGTTC  >  NC_000913/3468292‑3468485

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`