Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 107,553 (T)9→8 100% intergenic (+79/‑152) lpxC → / → secM UDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913107,5450T.96.2% 568.0 / 14.5 130intergenic (+71/‑160)lpxC/secMUDP‑3‑O‑acyl N‑acetylglucosamine deacetylase/regulator of secA translation
Reads supporting (aligned to +/- strand):  ref base T (4/1);  new base . (67/58);  total (71/59)
Fisher's exact test for biased strand distribution p-value = 3.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 142 total aligned reads displayed.

CAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAC  >  NC_000913/107447‑107640
                                                                                                   |                                                                                               
cAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtt                                                                                                <  1:2320388/100‑1 (MQ=255)
 aaaGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttt                                                                                               >  2:970920/1‑100 (MQ=255)
  aaGCGCCTTCAGCTGTACTGGTATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGTCACTCTCTCCGGCCAGGTGAACCAGTCGtttt                                                                                              <  2:610148/100‑1 (MQ=255)
    gcgcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttt                                                                                            >  1:3003271/1‑100 (MQ=255)
      gcCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGtttttttt                                                                                          <  2:1747397/100‑1 (MQ=255)
       ccTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGttttttttg                                                                                         <  1:2177320/100‑2 (MQ=255)
         ttCAGCTGTACTGGCATAACGACATTAATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCGCCGGCCAGGTGAACCAGTCGttttttttga                                                                                        >  2:353202/1‑97 (MQ=255)
           cAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAAtt                                                                                    >  2:2798396/1‑100 (MQ=255)
            agttGTACTGGCATAACGACATTTATATTGTCTTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTTAACCAGTCGTTTTTTTTGAAttt                                                                                   <  2:780676/97‑1 (MQ=255)
             gCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAAtttt                                                                                  <  2:126536/100‑1 (MQ=255)
               tGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTtat                                                                                <  2:195321/100‑1 (MQ=255)
               tGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTtat                                                                                <  2:296180/100‑1 (MQ=255)
                gTACTGGCATAACGACATTTATAATGTCGTATAAAATTCGACTGGCAAATCTGGCAATCTCTCCGGCTAGGAGAAGCAGTCGTTTTTTTTGAATTTtat                                                                                >  2:666977/1‑99 (MQ=255)
                 tACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATaa                                                                              >  2:2628258/1‑100 (MQ=255)
                  aCTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAag                                                                             <  2:2234279/100‑1 (MQ=255)
                   cTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAaga                                                                            >  1:3115967/1‑100 (MQ=255)
                   cTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAaga                                                                            >  1:1022370/1‑100 (MQ=255)
                     ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGc                                                                          <  2:687825/100‑1 (MQ=255)
                     ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGc                                                                          <  1:314088/100‑1 (MQ=255)
                     ggCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGc                                                                          >  2:249325/1‑100 (MQ=255)
                       cttAACGACATTTATACTGTTGTATAAAATTCCACTGGCAAATCTAGCACTCTCTGCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCta                                                                        <  1:2718157/98‑1 (MQ=255)
                       cATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCta                                                                        >  2:1351253/1‑100 (MQ=255)
                        aTAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCtat                                                                       >  1:859588/1‑100 (MQ=255)
                           aCGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATaaa                                                                    >  1:3165725/1‑100 (MQ=255)
                              aCATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATaaaaaa                                                                 >  2:2294647/1‑100 (MQ=255)
                               cATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAAc                                                                >  2:67901/1‑100 (MQ=255)
                                 tttATCCGGTCGAATAAAATTGGCTGGCAAAATTTGCAATTTTCTCCGGCAAGTGGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACgg                                                              <  2:2304657/100‑1 (MQ=255)
                                 tttATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACgg                                                              <  1:2453482/100‑1 (MQ=255)
                                  ttATACTGTCGTATAAAATTCGACTGGCAAATATGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACgg                                                              >  2:2600267/1‑99 (MQ=255)
                                    ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGc                                                           <  2:2365049/100‑1 (MQ=255)
                                    ataCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGc                                                           <  1:2016324/100‑1 (MQ=255)
                                     taCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCg                                                          >  1:2155396/1‑100 (MQ=255)
                                      aCTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCTGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAAAACTATAAAAAACGGTGCg                                                          <  2:387978/99‑1 (MQ=255)
                                         gTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACg                                                      <  2:1194339/100‑1 (MQ=255)
                                            gTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGTCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTg                                                   >  2:1742428/1‑100 (MQ=255)
                                            gTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTg                                                   >  1:2780583/1‑100 (MQ=255)
                                            gTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTg                                                   >  1:2624876/1‑100 (MQ=255)
                                             tataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGt                                                  <  2:2214887/100‑1 (MQ=255)
                                             tataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGt                                                  >  1:2476157/1‑100 (MQ=255)
                                             tataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGt                                                  >  1:2564193/1‑100 (MQ=255)
                                              ataAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTTATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtt                                                 >  2:2053524/1‑100 (MQ=255)
                                               taAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGagatcggaag                                                >  1:2215569/1‑91 (MQ=255)
                                               taAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGttt                                                >  2:251501/1‑100 (MQ=255)
                                                 tctttCGACGGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                              <  1:1009139/97‑1 (MQ=255)
                                                 aaaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGtttt                                               >  2:2761962/1‑99 (MQ=255)
                                                  aaTTCGACTGGCAGATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTc                                              <  2:2758970/99‑1 (MQ=255)
                                                  aaTTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCt                                             <  1:190890/100‑1 (MQ=255)
                                                  aaTTCGAAGGGCAAATCTGGCACTCTCTCCGACCAGGTGAACCAGTCGTTTTTTTTGAATTTTAAAAAAAAAAAAAAAAACGGTGCGAACGCTGTTTTCt                                             <  2:898247/100‑1 (MQ=255)
                                                    ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTcaga                                           >  2:1009134/1‑97 (MQ=255)
                                                    ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                           <  1:1018361/100‑1 (MQ=255)
                                                    ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTa                                           >  1:2999745/1‑100 (MQ=255)
                                                    ttCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCTTTTTTTTTTAATTTTATAAGAGCTATAAAAAACAGTGCGAACGCTGTTTTCTTa                                           >  1:1133764/1‑100 (MQ=255)
                                                     tCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTaa                                          <  1:2838164/100‑1 (MQ=255)
                                                      cGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAg                                         >  2:2706386/1‑100 (MQ=255)
                                                       gACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGc                                        >  1:786745/1‑100 (MQ=255)
                                                       gACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAAAAAAAAAAAAAACGGTGCGAACGCTGTTTTCTTAAGc                                        <  2:2439603/100‑1 (MQ=255)
                                                        aCTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAACa                                       >  1:2071/1‑100 (MQ=255)
                                                         cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                      <  2:209912/100‑1 (MQ=255)
                                                         cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                      >  1:1903952/1‑100 (MQ=255)
                                                         cTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCAc                                      >  2:1946875/1‑100 (MQ=255)
                                                          tGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACt                                     <  2:2041760/100‑1 (MQ=255)
                                                          tGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGAGAACGCTGTTTTCTTAAGCAc                                      >  2:405036/1‑99 (MQ=255)
                                                          tGGCAAATCTGGCACTATCTCCGGCCAGGTGAACCAGTAGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACt                                     <  1:2336027/100‑1 (MQ=255)
                                                           ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                    >  2:2508977/1‑100 (MQ=255)
                                                           ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                    >  1:1441667/1‑100 (MQ=255)
                                                           ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                    >  1:1771685/1‑100 (MQ=255)
                                                           ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                    >  1:831023/1‑100 (MQ=255)
                                                           ggCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtt                                    >  2:2720850/1‑100 (MQ=255)
                                                            gCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACttt                                   <  2:2389145/100‑1 (MQ=255)
                                                             cAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATACAAAACGGTGCGAACGCTGTTTTCTTAAGCACtttt                                  >  1:2854147/1‑100 (MQ=255)
                                                               aaaTCTTGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTc                                 <  1:3027087/100‑1 (MQ=255)
                                                               aaaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACtttt                                  >  1:2523625/1‑99 (MQ=255)
                                                               aaaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTc                                 >  1:712022/1‑100 (MQ=255)
                                                               aaaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTc                                 >  1:2523495/1‑100 (MQ=255)
                                                                aaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTcc                                >  2:2415718/1‑100 (MQ=255)
                                                                aaTCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTcc                                <  2:1110560/100‑1 (MQ=255)
                                                                    tGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcac                            <  2:1412580/100‑1 (MQ=255)
                                                                    tGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcac                            <  2:1158185/100‑1 (MQ=255)
                                                                      gctctctctCCGGCCAGGTGAACAAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGcac                            <  1:2756580/95‑1 (MQ=255)
                                                                      gCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACaa                          >  1:1417992/1‑100 (MQ=255)
                                                                         ctctctCCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACaagat                       >  2:2553977/1‑97 (MQ=255)
                                                                            tctcCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCGATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATc                    >  2:2592487/1‑100 (MQ=255)
                                                                             ctcCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCt                   >  1:1688217/1‑100 (MQ=255)
                                                                              tcCGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCt                   >  1:1578775/1‑99 (MQ=255)
                                                                               ccGGCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTc                 <  2:1130032/100‑1 (MQ=255)
                                                                                ctgCCAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCa                <  1:1129184/98‑1 (MQ=255)
                                                                                    cAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTc             <  2:790260/100‑1 (MQ=255)
                                                                                    cAGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCg            <  1:733360/100‑1 (MQ=255)
                                                                                     aGGTGAACCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGt           >  2:2175533/1‑100 (MQ=255)
                                                                                      ggTGAACCAGTCGTTTTTTTTTTTTTTTTTTAGTGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACACCTTATCTTCATTCGt           >  1:1399739/1‑99 (MQ=255)
                                                                                          aaCCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgt      <  2:3048187/100‑1 (MQ=255)
                                                                                           aCCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGATCGCTGTTTTCTTAAGCACTTTTCCGCACAACTGATCTTCATTCGTGCtgtn     <  2:1548213/100‑2 (MQ=255)
                                                                                           aCCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg     <  1:2628279/100‑1 (MQ=255)
                                                                                           aCCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg     <  1:3004812/100‑1 (MQ=255)
                                                                                           aCCAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCtgtg     <  1:740899/100‑1 (MQ=255)
                                                                                            ccAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTgg    <  1:249327/100‑1 (MQ=255)
                                                                                            ccAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTgg    <  1:1171326/100‑1 (MQ=255)
                                                                                             cAGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTgg    >  1:1427004/1‑99 (MQ=255)
                                                                                              aGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAc  <  2:2523607/100‑1 (MQ=255)
                                                                                              aGTCGTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAc  <  1:2600674/100‑1 (MQ=255)
                                                                                                   |                                                                                               
CAAAGCGCCTTCAGCTGTACTGGCATAACGACATTTATACTGTCGTATAAAATTCGACTGGCAAATCTGGCACTCTCTCCGGCCAGGTGAACCAGTCGTTTTTTTTTGAATTTTATAAGAGCTATAAAAAACGGTGCGAACGCTGTTTTCTTAAGCACTTTTCCGCACAACTTATCTTCATTCGTGCTGTGGAC  >  NC_000913/107447‑107640

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: