Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,951,617 Δ1 bp 33.5% pseudogene (76/663 nt) ilvG → pseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,951,6170G.33.5% 382.6 / 237.7 167pseudogene (76/663 nt)ilvGpseudogene, acetolactate synthase 2 large subunit, valine‑insensitive; acetolactate synthase II, large subunit, cryptic, interrupted
Reads supporting (aligned to +/- strand):  ref base G (52/58);  new base . (21/35);  total (73/94)
Fisher's exact test for biased strand distribution p-value = 2.51e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.60e-01

Only 100 of 176 total aligned reads displayed.

GCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCG  >  NC_000913/3951517‑3951716
                                                                                                     |                                                                                                    
gCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTAcgc                                                                                                     >  2:1024020/1‑100 (MQ=255)
 cATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcg                                                                                                      >  1:1473787/1‑100 (MQ=255)
 cATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTAcgc                                                                                                     >  1:315863/1‑99 (MQ=255)
    tACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTAcgccg                                                                                                   >  1:1166938/1‑98 (MQ=255)
     aCAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCgt                                                                                                >  2:1434848/1‑100 (MQ=255)
      cagcagCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCgtt                                                                                               >  2:1737412/1‑100 (MQ=255)
      cagcagCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCgtt                                                                                               >  2:2047217/1‑100 (MQ=255)
           gcCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCgttgttgt                                                                                          >  1:715810/1‑100 (MQ=255)
            ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgttg                                                                                           <  1:1278036/100‑1 (MQ=255)
            ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCgttgttg                                                                                           <  2:1762917/100‑1 (MQ=255)
            ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCgttgttgtt                                                                                         <  1:3180849/100‑1 (MQ=255)
            ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCgttgttgtt                                                                                         <  2:258687/100‑1 (MQ=255)
            ccGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCgttgttgtt                                                                                         <  2:1664553/100‑1 (MQ=255)
              gTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCG‑‑GACGCTATCTACGCGCCGTTGTTGTTaa                                                                                       <  1:1979176/100‑1 (MQ=255)
               ttAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTaaa                                                                                      <  2:1517633/100‑1 (MQ=255)
                tAAATCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTa                                                                                        <  1:2814133/100‑1 (MQ=255)
                 aaaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAaac                                                                                    <  2:2949287/100‑1 (MQ=255)
                 aaaTCAATGACTGACAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAaac                                                                                    <  1:2454740/100‑1 (MQ=255)
                  aaTCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTaaa                                                                                      >  1:229289/1‑100 (MQ=255)
                  aaTCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAaaca                                                                                   >  1:1866697/1‑100 (MQ=255)
                    tCAATGACTGGCAGCAACACTGCAGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaac                                                                                    <  1:1936992/100‑1 (MQ=255)
                    tCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAaaca                                                                                   <  1:1434529/100‑1 (MQ=255)
                      aaTGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTTTaacaac                                                                                 <  1:1030180/100‑1 (MQ=255)
                      aaTGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTg                                                                               >  1:2950255/1‑100 (MQ=255)
                        tGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTg                                                                               >  2:74558/1‑100 (MQ=255)
                          aCTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCgg                                                                           <  1:204922/100‑1 (MQ=255)
                           cTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCGACGCGCCGTTGTTGTTAAAACAACTGTCg                                                                            >  2:1945547/1‑100 (MQ=255)
                             ggCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATc                                                                        >  1:2522664/1‑100 (MQ=255)
                             ggCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATc                                                                        >  2:2269793/1‑100 (MQ=255)
                             ggCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATc                                                                        >  1:2724044/1‑100 (MQ=255)
                              gcagcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCg                                                                       <  1:3192519/100‑1 (MQ=255)
                              gcagcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCg                                                                       <  1:3188070/100‑1 (MQ=255)
                              gcagcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCg                                                                       <  2:2661541/100‑1 (MQ=255)
                               cagcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGt                                                                      >  1:1300564/1‑100 (MQ=255)
                                 gcaACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTaa                                                                    <  1:133378/100‑1 (MQ=255)
                                     cacTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCt                                                                >  1:989132/1‑100 (MQ=255)
                                       cTGCGCGCAGCTGCGTGATGAACATTCATGGCGTTAAGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAAcc                                                                 >  2:889261/1‑99 (MQ=255)
                                        tGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg                                                             <  1:85118/100‑1 (MQ=255)
                                         gcgcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCgg                                                            <  2:2983906/100‑1 (MQ=255)
                                          cgcgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCg                                                             <  1:394175/100‑1 (MQ=255)
                                             cgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGa                                                           <  2:2628666/100‑1 (MQ=255)
                                             cgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGAtt                                                         >  2:1651365/1‑100 (MQ=255)
                                             cgcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGAtt                                                         <  1:1651375/100‑1 (MQ=255)
                                              gcAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTg                                                        <  1:1630994/100‑1 (MQ=255)
                                                 gCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGc                                                       >  2:1473643/1‑100 (MQ=255)
                                                 gCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgt                                                     >  1:2603723/1‑100 (MQ=255)
                                                   tGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTCAAACAACTGTCGGATCGTAACCCTGCGGATTGcgtcg                                                   >  1:1759520/1‑100 (MQ=255)
                                                   tGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcg                                                   <  1:938097/100‑1 (MQ=255)
                                                     cGTGATGAACATTCCTGGCTTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCTGATCGTAAACCTGCGGATTGcgtcg                                                   <  2:1095142/100‑1 (MQ=255)
                                                     cGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCATAAACCTGCGGATTGcgtcg                                                   <  2:1990243/100‑1 (MQ=255)
                                                     cGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTg                                                 <  1:442954/100‑1 (MQ=255)
                                                     cGTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTg                                                 >  2:1201326/1‑100 (MQ=255)
                                                      gTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTg                                                 <  1:1067776/99‑1 (MQ=255)
                                                      gTGATGAACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGa                                                <  1:1096933/100‑1 (MQ=255)
                                                       tgatgaACATTCCTAGCGTTACGACCATCCCGGGGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGcgtcg                                                   <  1:1200068/98‑1 (MQ=255)
                                                        gatgaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGa                                                >  2:509139/1‑100 (MQ=255)
                                                         atgaACATTCCTGGCGTTACGACCATCCTGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACca                                             >  1:2905175/1‑100 (MQ=255)
                                                          tgaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGAcc                                              <  1:1009056/100‑1 (MQ=255)
                                                          tgaACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcac                                            <  2:738703/100‑1 (MQ=255)
                                                           gaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACca                                             >  1:2899133/1‑100 (MQ=255)
                                                           gaACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcaca                                           >  1:224305/1‑100 (MQ=255)
                                                           gaACATTCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACcaca                                           >  1:148379/1‑100 (MQ=255)
                                                              cATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAt                                        >  1:1824336/1‑100 (MQ=255)
                                                                 tCCTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtgtg                                    <  1:2269809/100‑1 (MQ=255)
                                                                  ccTGGCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTgg                                   <  2:3251363/100‑1 (MQ=255)
                                                                   cTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGAtgtg                                    >  2:1606248/1‑100 (MQ=255)
                                                                   cTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTggg                                  >  1:3062979/1‑100 (MQ=255)
                                                                   cTGGCGTTACGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTggg                                  <  1:2337318/100‑1 (MQ=255)
                                                                    tGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTgg                                   <  1:74560/100‑1 (MQ=255)
                                                                     ggCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgc                                <  2:369723/100‑1 (MQ=255)
                                                                      gCGTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgca                               <  2:2404596/100‑1 (MQ=255)
                                                                        gTTACGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcagc                             <  2:2560652/100‑1 (MQ=255)
                                                                          tACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGgcagc                             >  1:1957503/1‑100 (MQ=255)
                                                                           aCGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAcc                          <  1:1248895/100‑1 (MQ=255)
                                                                           aCGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAcc                          <  2:809822/100‑1 (MQ=255)
                                                                           aCGACCATCCCGGTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAcc                          <  1:510494/100‑1 (MQ=255)
                                                                            cGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCAc                           >  2:3104909/1‑100 (MQ=255)
                                                                            cGACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCa                         >  1:500484/1‑100 (MQ=255)
                                                                             gACCATCCCGGT‑‑CGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCa                         <  2:2843684/99‑1 (MQ=255)
                                                                              aCCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCa                         >  2:616891/1‑100 (MQ=255)
                                                                              aCCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCa                         <  1:3005093/100‑1 (MQ=255)
                                                                                cATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTTGGGGCAGCACCAg                        >  1:2362886/1‑100 (MQ=255)
                                                                                 aTCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAt                      >  1:3065876/1‑100 (MQ=255)
                                                                                  tCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAtg                     <  1:1813573/100‑1 (MQ=255)
                                                                                    tcccggtcGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAtgtg                   <  2:2523533/93‑1 (MQ=255)
                                                                                      ggTGACGCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCt               >  2:782988/1‑100 (MQ=255)
                                                                                       tggtcGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGc                <  2:2905150/96‑1 (MQ=255)
                                                                                       gTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGAtgtg                   <  1:1119704/96‑1 (MQ=255)
                                                                                        tGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGTATTGCGTCGTGACCACAGATGTGGCGCAGCACCCGAtgttgtct               >  2:3175484/1‑95 (MQ=255)
                                                                                            gCTATCTACGCGCTGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTTACCACAGATGTGGGTCAGCACCAGATGTGGGCTtcgc           >  2:2155546/1‑100 (MQ=255)
                                                                                            gCTATCTACGCGCCGTTGTTGCTAAAACAACTTTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATCTCCAGCCGCACCAGATGTGGGCTgc             >  2:2767446/1‑98 (MQ=255)
                                                                                            gCTATCTA‑‑CGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCgcag         <  2:2522646/100‑1 (MQ=255)
                                                                                             cTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCgca          <  2:1569027/100‑1 (MQ=255)
                                                                                             cTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCgca          <  1:2825894/100‑1 (MQ=255)
                                                                                               aTCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCgcagc        <  2:1288978/100‑1 (MQ=255)
                                                                                                 cTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGcac      <  2:2212834/100‑1 (MQ=255)
                                                                                                  tACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGcaca     >  1:1726092/1‑100 (MQ=255)
                                                                                                  tACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGcaca     >  2:2410137/1‑100 (MQ=255)
                                                                                                    cgcgCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATc   <  2:2724021/100‑1 (MQ=255)
                                                                                                    cgcgCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATc   >  1:1766875/1‑100 (MQ=255)
                                                                                                     |                                                                                                    
GCATTACAGCAGCCGTTAAATCAATGACTGGCAGCAACACTGCGCGCAGCTGCGTGATGAACATTCCTGGCGTTACGACCATCCCGGTGACGCTATCTACGCGCCGTTGTTGTTAAAACAACTGTCGGATCGTAAACCTGCGGATTGCGTCGTGACCACAGATGTGGGGCAGCACCAGATGTGGGCTGCGCAGCACATCG  >  NC_000913/3951517‑3951716

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: