Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,755,639 G→A 100% intergenic (‑498/‑59) ydhZ ← / → pykF uncharacterized protein/pyruvate kinase I

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,755,6390GA98.3% 401.2 / ‑2.7 119intergenic (‑498/‑59)ydhZ/pykFuncharacterized protein/pyruvate kinase I
Reads supporting (aligned to +/- strand):  ref base G (0/2);  new base A (63/53);  total (64/55)
Fisher's exact test for biased strand distribution p-value = 2.15e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.71e-01

Only 100 of 119 total aligned reads displayed.

GCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGA  >  NC_000913/1755541‑1755734
                                                                                                  |                                                                                               
gCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGc                                                                                                <  1:2739691/100‑1 (MQ=255)
     gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgc                                                                                           >  1:2957562/1‑100 (MQ=255)
     gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgc                                                                                           <  1:2620425/100‑1 (MQ=255)
     gaCACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgc                                                                                           >  2:321907/1‑100 (MQ=255)
       cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc                                                                                         <  1:188838/100‑1 (MQ=255)
       cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc                                                                                         >  1:2233117/1‑100 (MQ=255)
       cacaAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTgcgc                                                                                         >  2:3134516/1‑100 (MQ=255)
         caAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGcct                                                                                       <  2:390694/100‑2 (MQ=255)
          aaGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCa                                                                                      >  1:1271889/1‑100 (MQ=255)
          aaGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCa                                                                                      <  2:2884926/100‑1 (MQ=255)
           aGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAg                                                                                     <  1:2581764/100‑1 (MQ=255)
               cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAg                                                                                 >  1:465698/1‑100 (MQ=255)
               cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAg                                                                                 <  1:1958257/100‑1 (MQ=255)
               cacaTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAg                                                                                 <  2:2903836/100‑1 (MQ=255)
                  aTTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCaa                                                                              <  1:2807533/100‑1 (MQ=255)
                     ccTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGtt                                                                           >  1:1177889/1‑100 (MQ=255)
                      ctctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGttt                                                                          >  1:3057579/1‑100 (MQ=255)
                        ctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTtct                                                                        <  2:2598265/100‑1 (MQ=255)
                        ctGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTtct                                                                        >  1:2810313/1‑100 (MQ=255)
                          gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                      >  1:2172484/1‑100 (MQ=255)
                          gCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTcc                                                                      <  1:1624951/100‑1 (MQ=255)
                           cACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTccc                                                                     >  2:2533033/1‑100 (MQ=255)
                               cTTTTTCGATGTCACCTATTCTTAGAGCGAGCCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATcc                                                                 <  1:954962/100‑1 (MQ=255)
                                 ttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCtt                                                               >  1:321602/1‑100 (MQ=255)
                                 ttttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCtt                                                               >  2:2663924/1‑100 (MQ=255)
                                  tttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtc                                                              <  1:306378/100‑1 (MQ=255)
                                  tttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtc                                                              >  2:1049257/1‑100 (MQ=255)
                                   ttCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtct                                                             <  1:391018/100‑1 (MQ=255)
                                    tCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTtctc                                                            <  2:3139162/100‑1 (MQ=255)
                                     cGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCa                                                           <  2:2370298/100‑1 (MQ=255)
                                       ctGTCACCTATCCTTAGAGCGCGGCACCACCACTTCCGTAATACCGCATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAc                                                         <  2:1073483/99‑1 (MQ=255)
                                       aTGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAAc                                                         <  2:2349634/100‑1 (MQ=255)
                                          tCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTa                                                      <  2:1066330/100‑1 (MQ=255)
                                           cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTaa                                                     >  2:2854169/1‑100 (MQ=255)
                                           cACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTAAACTTaa                                                     >  1:1074574/1‑100 (MQ=255)
                                             ccTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACATTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaag                                                   >  2:2156405/1‑100 (MQ=255)
                                               tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagac                                                 >  1:1661715/1‑100 (MQ=255)
                                               tATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagac                                                 <  1:1504844/100‑1 (MQ=255)
                                                aTCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagact                                                >  2:921506/1‑100 (MQ=255)
                                                aTCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagact                                                >  1:1322965/1‑100 (MQ=255)
                                                 tCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagacta                                               >  1:1731577/1‑100 (MQ=255)
                                                 tCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagacta                                               <  2:2810289/100‑1 (MQ=255)
                                                  ccTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaa                                              >  2:304961/1‑100 (MQ=255)
                                                  ccTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaa                                              >  2:1848317/1‑100 (MQ=255)
                                                  ccTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTATCAACTTAaagactaa                                              >  2:2477278/1‑100 (MQ=255)
                                                   cTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaag                                             >  1:684757/1‑100 (MQ=255)
                                                     tAGATCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaagac                                           <  1:1102268/100‑1 (MQ=255)
                                                     tAGAGCGATGCACCACCATTTTCGTAATTCCTGATTCGCTTTCCAACAGTGCGCTCAGAAATCAATTTTCTCCCATCCTTCTCAACTTAaagactaagac                                           <  2:1467325/100‑1 (MQ=255)
                                                     tAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAaagactaagac                                           >  2:2600480/1‑100 (MQ=255)
                                                       gagCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTg                                         <  1:1269940/100‑1 (MQ=255)
                                                          cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                      >  2:1104170/1‑100 (MQ=255)
                                                          cGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCa                                      <  2:953719/100‑1 (MQ=255)
                                                           gAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCAt                                     >  1:2718160/1‑100 (MQ=255)
                                                           gAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCAt                                     >  2:1479496/1‑100 (MQ=255)
                                                             ggCACCACCACTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGa                                   <  2:445509/100‑1 (MQ=255)
                                                              gCAACACCACGTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCACATCCTTCTCAACTTAAAGACTAAGACTGTCAt                                     <  2:1610763/97‑1 (MQ=255)
                                                               caccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaa                                 >  1:2177686/1‑100 (MQ=255)
                                                                accaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaa                                >  2:527655/1‑100 (MQ=255)
                                                                accaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaa                                >  1:1644008/1‑100 (MQ=255)
                                                                 ccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaaa                               >  1:2509188/1‑100 (MQ=255)
                                                                 ccaccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGaaaaa                               >  2:2798457/1‑100 (MQ=255)
                                                                  caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg                              <  1:47606/100‑1 (MQ=255)
                                                                  caccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAg                              >  2:152735/1‑100 (MQ=255)
                                                                   accacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGa                             >  2:1521430/1‑100 (MQ=255)
                                                                   accacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGa                             >  2:3250349/1‑100 (MQ=255)
                                                                    ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc                            >  2:96610/1‑100 (MQ=255)
                                                                    ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc                            <  1:1011725/100‑1 (MQ=255)
                                                                    ccacTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGAc                            <  1:111949/100‑1 (MQ=255)
                                                                      acTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCa                          >  1:90625/1‑100 (MQ=255)
                                                                      acTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCa                          <  2:2233101/100‑1 (MQ=255)
                                                                      acTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCa                          >  1:2636005/1‑100 (MQ=255)
                                                                       cTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaa                         >  1:1088841/1‑100 (MQ=255)
                                                                       cTTTCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaa                         >  2:312679/1‑100 (MQ=255)
                                                                        tttCGTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCaaa                        >  1:2820616/1‑100 (MQ=255)
                                                                            gTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAAt                      >  1:2837633/1‑98 (MQ=255)
                                                                            gTAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTg                    <  1:1056626/100‑1 (MQ=255)
                                                                             tAATACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGt                   >  2:2392569/1‑100 (MQ=255)
                                                                              aaTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGtt                  <  2:940971/100‑1 (MQ=255)
                                                                              aaTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGtt                  <  1:2256669/100‑1 (MQ=255)
                                                                              aaTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGtt                  <  2:2727716/100‑1 (MQ=255)
                                                                               aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                 <  1:1049262/100‑1 (MQ=255)
                                                                               aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                 <  1:1155792/100‑1 (MQ=255)
                                                                               aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                 <  1:2028103/100‑1 (MQ=255)
                                                                               aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                 <  1:3207210/100‑1 (MQ=255)
                                                                               aTACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                 <  2:1312243/100‑1 (MQ=255)
                                                                               aTACCGGATTCGCTTTCCGACACTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGttt                 <  2:2429717/100‑1 (MQ=255)
                                                                                tACCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTg                >  1:1361437/1‑100 (MQ=255)
                                                                                 aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc               <  1:1489722/100‑1 (MQ=255)
                                                                                 aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc               >  2:2162496/1‑100 (MQ=255)
                                                                                 aCCGGATTCGCTTTCCGACAGTGCGCCCAGAAAACAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGc               >  2:1951431/1‑100 (MQ=255)
                                                                                  ccGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCa              >  1:959924/1‑100 (MQ=255)
                                                                                  ccGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCa              >  1:2873350/1‑100 (MQ=255)
                                                                                  ccGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCa              >  1:554558/1‑100 (MQ=255)
                                                                                   cGGATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAc             <  1:304963/100‑1 (MQ=255)
                                                                                    ggATTCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCAcc            <  2:531820/100‑1 (MQ=255)
                                                                                       ttCGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATc         <  2:3192378/100‑1 (MQ=255)
                                                                                         cGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCgg       >  1:3125700/1‑100 (MQ=255)
                                                                                         cGCTTTCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCgg       >  2:964518/1‑100 (MQ=255)
                                                                                            tttCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGAcc    <  1:1521439/100‑1 (MQ=255)
                                                                                              tCCGACAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGa  <  2:1036365/100‑1 (MQ=255)
                                                                                                  |                                                                                               
GCTGAGACACAAGCACACATTCCTCTGCACGCTTTTTCGATGTCACCTATCCTTAGAGCGAGGCACCACCACTTTCGTAATACCGGATTCGCTTTCCGGCAGTGCGCCCAGAAAGCAAGTTTCTCCCATCCTTCTCAACTTAAAGACTAAGACTGTCATGAAAAAGACCAAAATTGTTTGCACCATCGGACCGA  >  NC_000913/1755541‑1755734

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: