Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,469,733 (T)8→7 coding (603/1332 nt) gtrS → serotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,469,7260T.96.6% 632.5 / 17.0 147coding (596/1332 nt)gtrSserotype‑specific glucosyl transferase, CPS‑53 (KpLE1) prophage
Reads supporting (aligned to +/- strand):  ref base T (4/1);  new base . (63/78);  total (68/79)
Fisher's exact test for biased strand distribution p-value = 1.80e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.53e-01

Only 100 of 174 total aligned reads displayed.

ACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTG  >  NC_000913/2469627‑2469821
                                                                                                   |                                                                                               
aCAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACACCACTAATAGTGTATTCatattatat                                                                                                 >  2:1253264/1‑100 (MQ=255)
 cgaTAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCatattatatt                                                                                                >  2:2616049/3‑100 (MQ=255)
  aaTAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttt                                                                                               >  1:1596054/1‑100 (MQ=255)
  aaTAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttt                                                                                               >  1:2033377/1‑100 (MQ=255)
   aTAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttt                                                                                              >  1:2424648/1‑100 (MQ=255)
     aaaTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttt                                                                                            <  1:528380/100‑1 (MQ=255)
     aaaTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttt                                                                                            <  2:2247003/100‑1 (MQ=255)
      aaTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt                                                                                           <  1:392732/100‑1 (MQ=255)
      aaTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAttttttt                                                                                           >  2:795180/1‑100 (MQ=255)
       aTCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttg                                                                                          <  1:2689344/100‑2 (MQ=255)
        tCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttga                                                                                         <  1:1389801/100‑3 (MQ=255)
         cTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttgat                                                                                        <  2:1337489/100‑4 (MQ=255)
          tATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATAtttttttgata                                                                                       >  2:1192702/1‑96 (MQ=255)
           aTGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAg                                                                                     <  1:1674554/100‑1 (MQ=255)
             gCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGac                                                                                   >  1:1320159/1‑100 (MQ=255)
             gCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGac                                                                                   <  1:75990/100‑1 (MQ=255)
                 ttGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACaa                                                                               >  1:2037742/1‑100 (MQ=255)
                   gCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAAc                                                                             <  2:575209/100‑1 (MQ=255)
                   gCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAAc                                                                             >  1:650814/1‑100 (MQ=255)
                   gCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAAc                                                                             >  2:2769842/1‑100 (MQ=255)
                   gCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAAc                                                                             >  1:1729882/1‑100 (MQ=255)
                   gCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTAAATTTTTTTGATACaaacaaac                                                                             >  2:700140/1‑100 (MQ=255)
                      aTTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTATATTCACAAACTaa                                                                          >  1:1129999/1‑100 (MQ=255)
                          ttttCGTTGCTACTAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAattat                                                                      <  2:791612/100‑1 (MQ=255)
                            ttCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAAttatta                                                                    <  1:1356637/100‑1 (MQ=255)
                              cGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTaaa                                                                  >  1:2609931/1‑100 (MQ=255)
                              cGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTaaa                                                                  <  1:1626791/100‑1 (MQ=255)
                              cGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAAATATTaaa                                                                  <  2:1490946/100‑1 (MQ=255)
                              cGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCAATAATAGTGTATTCATATTCTATTTTTTTGATAGACACAAATTAATTATTaaa                                                                  >  1:1975086/1‑100 (MQ=255)
                               gTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAAt                                                                 >  2:1634851/1‑100 (MQ=255)
                               gTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAAt                                                                 <  1:1216657/100‑1 (MQ=255)
                                ttGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATc                                                                <  1:1190615/100‑1 (MQ=255)
                                 tGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCt                                                               >  1:1258884/1‑100 (MQ=255)
                                  gCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCt                                                               >  1:456769/1‑99 (MQ=255)
                                  gCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTg                                                              <  1:2405625/100‑1 (MQ=255)
                                  gCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTg                                                              >  2:154299/1‑100 (MQ=255)
                                  gCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTg                                                              >  2:232917/1‑100 (MQ=255)
                                   cTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGt                                                             >  2:1085314/1‑100 (MQ=255)
                                   cTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGt                                                             >  2:1472917/1‑100 (MQ=255)
                                   cTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGt                                                             >  1:482684/1‑100 (MQ=255)
                                   cTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGt                                                             >  2:2772006/1‑100 (MQ=255)
                                   cTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGt                                                             <  1:483317/100‑1 (MQ=255)
                                   cTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGt                                                             <  1:1570344/100‑1 (MQ=255)
                                   cTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGt                                                             >  1:1772753/1‑100 (MQ=255)
                                    taataaTATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTa                                                            >  1:1007723/1‑100 (MQ=255)
                                     aataatATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTaa                                                           <  2:2489150/100‑1 (MQ=255)
                                     aataatATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTaa                                                           <  1:2771980/100‑1 (MQ=255)
                                      ataataTCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAat                                                          >  2:53633/1‑100 (MQ=255)
                                       taataTCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAata                                                         <  2:388791/100‑1 (MQ=255)
                                             cTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgtg                                                   <  1:1309836/100‑1 (MQ=255)
                                              tACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgtgg                                                  >  2:2220155/1‑100 (MQ=255)
                                              tACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgtgg                                                  <  2:1329031/100‑1 (MQ=255)
                                                cTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgtggtg                                                <  2:2570284/100‑1 (MQ=255)
                                                cTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCgtggtg                                                <  2:857832/100‑1 (MQ=255)
                                                  gCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGgtgt                                               >  1:109846/1‑99 (MQ=255)
                                                  gCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGtgtg                                              <  2:1765523/100‑1 (MQ=255)
                                                  gCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTACTCAATCTGTACTATGCgtggcct                                               >  2:1852884/1‑96 (MQ=255)
                                                   cTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgc                                             <  2:897615/100‑1 (MQ=255)
                                                     aaaaaTCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgctt                                           <  2:1585901/100‑1 (MQ=255)
                                                        aaTCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgcttgct                                        >  2:369935/1‑100 (MQ=255)
                                                        aaTCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGtgcttgct                                        <  1:97416/100‑1 (MQ=255)
                                                          tCAATTTATATTAACCCCACTAATAGTGTATTGATAGTATATTTTTTTGATAGAGACAATCTAATTATTAAATATGTAATATGCGTGGTGTgattgctt                                       >  1:1345370/1‑99 (MQ=255)
                                                          tCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGcttgcttg                                      <  2:1130007/100‑1 (MQ=255)
                                                          tCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGcttgcttg                                      <  2:2638551/100‑1 (MQ=255)
                                                           cAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCttgcttgc                                     >  2:1467773/1‑100 (MQ=255)
                                                            aaTTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCg                                    <  2:1262691/100‑1 (MQ=255)
                                                             aTTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGt                                   >  1:1721244/1‑100 (MQ=255)
                                                             aTTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGt                                   >  2:1993658/1‑100 (MQ=255)
                                                              tttATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTc                                  <  1:1122576/100‑1 (MQ=255)
                                                              tttATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTc                                  <  2:648080/100‑1 (MQ=255)
                                                                tataTTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTATAGACTCAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCaa                                >  1:2324530/1‑100 (MQ=255)
                                                                tataTTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCaa                                >  2:2417244/1‑100 (MQ=255)
                                                                   atTAACCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATACGTGGTGTGCTTGCTTGCGTCAatat                             >  1:854336/1‑100 (MQ=255)
                                                                      aaCCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTg                          <  1:89067/100‑1 (MQ=255)
                                                                      aaCCCCACTAATAGTGTATTCATATTATATTTGTTTGATCGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTg                          <  1:1984732/100‑1 (MQ=255)
                                                                       aCCCCACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGc                         >  2:2808382/1‑100 (MQ=255)
                                                                        ccccACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCa                        <  2:2595533/100‑1 (MQ=255)
                                                                        ccccACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCa                        <  2:1040814/100‑1 (MQ=255)
                                                                         nccACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCaa                       >  1:1262624/2‑100 (MQ=255)
                                                                         cccACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCaa                       >  2:152423/1‑100 (MQ=255)
                                                                         cccACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCaa                       <  2:2525371/100‑1 (MQ=255)
                                                                         cccACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCaa                       <  1:1906153/100‑1 (MQ=255)
                                                                           cACTAATAGTGTATTCATATTATATTTTTTTTATAGACACAAACTAATTATTAAATCTGTAATATGCTTGGTGTGCTTGCTTGCGTCAATATTTGCAata                     >  1:1469112/1‑100 (MQ=255)
                                                                           cACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAata                     <  1:700134/100‑1 (MQ=255)
                                                                           cACTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAata                     <  1:2769816/100‑1 (MQ=255)
                                                                            aCTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAatat                    >  2:962995/1‑100 (MQ=255)
                                                                             cTAATAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATc                   >  2:2306026/1‑100 (MQ=255)
                                                                                aTAGTGTATTCATATTATATTTTTTTGATAGACTCAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTa                >  1:216816/1‑100 (MQ=255)
                                                                                 tAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTAt               >  2:1276633/1‑100 (MQ=255)
                                                                                 tAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTAt               <  1:2306007/100‑1 (MQ=255)
                                                                                 tAGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTAt               <  1:1085306/100‑1 (MQ=255)
                                                                                  aGTGTATTCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTAtt              <  1:1993643/100‑1 (MQ=255)
                                                                                   gtgtATTGATATTATATTTTGGTGAGAGACGCAAACTAATTATTAAATCTGTAATATGCGTGGGGTGCTTGCTTGCGTCATTATTTGCGATATCTTAt               <  1:2416262/98‑1 (MQ=255)
                                                                                      tATTCATATTATATTTTTTTGATAGACACAAACTAATAATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTAtt              >  2:1816086/1‑96 (MQ=255)
                                                                                        ttCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAg        <  2:2170721/100‑1 (MQ=255)
                                                                                         tCATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAgg       <  2:366247/100‑1 (MQ=255)
                                                                                          cATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGgt      >  2:550478/1‑100 (MQ=255)
                                                                                          cATATTATATTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGgt      >  1:2436008/1‑100 (MQ=255)
                                                                                              ttatattTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGtgttg  <  1:795174/100‑1 (MQ=255)
                                                                                              ttatattTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGtgttg  >  1:2286119/1‑100 (MQ=255)
                                                                                                   |                                                                                               
ACAATAAATCTATGCTTTTGCTATTTTTTTCGTTGCTAATAATATCTACTGCTAAAAATCAATTTATATTAACCCCACTAATAGTGTATTCATATTATATTTTTTTTGATAGACACAAACTAATTATTAAATCTGTAATATGCGTGGTGTGCTTGCTTGCGTCAATATTTGCAATATCTTATTCAAAAGGTGTTG  >  NC_000913/2469627‑2469821

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: