Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,595,315 (A)5→4 coding (166/1278 nt) livM ← branched‑chain amino acid ABC transporter permease

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,595,3110A.95.5% 465.1 / 14.8 110coding (170/1278 nt)livMbranched‑chain amino acid ABC transporter permease
Reads supporting (aligned to +/- strand):  ref base A (3/2);  new base . (48/57);  total (51/59)
Fisher's exact test for biased strand distribution p-value = 6.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 118 total aligned reads displayed.

TTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCAC  >  NC_000913/3595212‑3595405
                                                                                                   |                                                                                              
ttCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGgaaga                                                                                                >  2:1871427/1‑100 (MQ=255)
   tGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaa                                                                                             >  2:1815910/1‑100 (MQ=255)
    gCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaag                                                                                            >  2:2607890/1‑99 (MQ=255)
    gCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaag                                                                                            <  2:2706663/100‑2 (MQ=255)
      ttCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaagac                                                                                          >  1:509462/1‑97 (MQ=255)
       tCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGaaaagacc                                                                                         <  1:588067/100‑5 (MQ=255)
          ccGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGaccacc                                                                                     >  2:1220161/1‑100 (MQ=255)
          ccGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGaccacc                                                                                     >  1:52380/1‑100 (MQ=255)
            gTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCAccgc                                                                                   <  1:480743/100‑1 (MQ=255)
                  ccATCAATGGCGGGCAGAATAATCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGcc                                                                             <  1:1184320/100‑1 (MQ=255)
                  ccATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGcc                                                                             <  1:1014759/100‑1 (MQ=255)
                  ccATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGcc                                                                             <  1:180555/100‑1 (MQ=255)
                   cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCg                                                                            >  1:2478303/1‑100 (MQ=255)
                   cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCg                                                                            >  2:2018793/1‑100 (MQ=255)
                   cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCg                                                                            <  1:1889572/100‑1 (MQ=255)
                   cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCg                                                                            >  1:1241076/1‑100 (MQ=255)
                   cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCg                                                                            <  2:2371956/100‑1 (MQ=255)
                   cATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACACCGCCGTGCCGa                                                                           >  1:1241067/1‑100 (MQ=255)
                    aTCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGa                                                                           >  1:1291319/1‑100 (MQ=255)
                     tCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGAt                                                                          <  1:410330/100‑1 (MQ=255)
                      cAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATa                                                                         <  1:2820430/100‑1 (MQ=255)
                        aTGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATaaa                                                                       >  2:2625399/1‑100 (MQ=255)
                         tGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAac                                                                      <  1:1269315/100‑1 (MQ=255)
                            cGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACGCTTTCTGGAAAGCCGGTCGGAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACAcc                                                                   <  1:1545898/100‑1 (MQ=255)
                            cGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACAcc                                                                   >  1:2046796/1‑100 (MQ=255)
                            cGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACAcc                                                                   >  2:148474/1‑100 (MQ=255)
                             gggCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACAccc                                                                  <  2:491151/100‑1 (MQ=255)
                                 aGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTg                                                              <  2:2328598/100‑1 (MQ=255)
                                  gAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGc                                                             <  2:2412321/100‑1 (MQ=255)
                                   aaTAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGcn                                                            <  2:602708/100‑2 (MQ=255)
                                   aaTAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGcc                                                            >  2:109625/1‑100 (MQ=255)
                                    aTAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCa                                                           <  1:1577813/100‑1 (MQ=255)
                                     tAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCaa                                                          >  2:143106/1‑100 (MQ=255)
                                      aaaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAAc                                                         <  2:1295716/100‑1 (MQ=255)
                                      aaaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAAc                                                         <  2:2449966/100‑1 (MQ=255)
                                       aaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACg                                                        >  1:2724919/1‑100 (MQ=255)
                                       aaCTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACg                                                        >  1:2602885/1‑100 (MQ=255)
                                         cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                      >  2:1322456/1‑100 (MQ=255)
                                         cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                      <  2:2015081/100‑1 (MQ=255)
                                         cTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGa                                                      >  2:2614421/1‑100 (MQ=255)
                                          ttCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGAc                                                     <  2:167516/100‑1 (MQ=255)
                                             ggTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCACCGCACAt                                                   >  2:549287/1‑99 (MQ=255)
                                              gTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATc                                                  <  2:2505760/100‑1 (MQ=255)
                                              gTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATcc                                                 <  1:1290092/100‑1 (MQ=255)
                                              gTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATc                                                  <  2:1077993/99‑1 (MQ=255)
                                                  ggAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAg                                             <  2:1225636/100‑1 (MQ=255)
                                                   gAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGc                                            >  2:2338930/1‑100 (MQ=255)
                                                    aaaCGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGcc                                           <  2:610511/100‑1 (MQ=255)
                                                        gCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCgtgt                                       <  1:2607869/100‑1 (MQ=255)
                                                         cTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc                                      <  2:2328173/100‑1 (MQ=255)
                                                         cTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc                                      <  1:109624/100‑1 (MQ=255)
                                                         cTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc                                      <  2:1032498/100‑1 (MQ=255)
                                                         cTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTc                                      >  1:1500547/1‑100 (MQ=255)
                                                          ttttCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCg                                     >  1:374348/1‑100 (MQ=255)
                                                          ttttCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCg                                     <  1:423578/100‑1 (MQ=255)
                                                             tCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGCCCACCGCCGTGCCGATAAACACACACTGCGAACGGACATCCGAAGCCGTATCGacc                                  >  1:1946496/1‑100 (MQ=255)
                                                             tCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacc                                  <  1:2164542/100‑1 (MQ=255)
                                                             tCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacc                                  <  1:2821262/100‑1 (MQ=255)
                                                             tCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacc                                  >  2:1707603/1‑100 (MQ=255)
                                                             tCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacc                                  <  2:273166/100‑1 (MQ=255)
                                                              cAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacca                                 <  2:52381/100‑1 (MQ=255)
                                                              cAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacca                                 >  1:1250293/1‑100 (MQ=255)
                                                              cAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGacca                                 >  1:519854/1‑100 (MQ=255)
                                                               aaCCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGaccac                                >  2:95056/1‑100 (MQ=255)
                                                                tcccTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGaccacc                               <  2:2087708/99‑1 (MQ=255)
                                                                 cccTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGAccacca                              >  1:2087693/1‑100 (MQ=255)
                                                                  ccTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAg                             <  2:435761/100‑1 (MQ=255)
                                                                  ccTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAg                             >  2:1830133/1‑100 (MQ=255)
                                                                  ccTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAg                             >  1:1921964/1‑100 (MQ=255)
                                                                   cTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGt                            >  1:1425298/1‑100 (MQ=255)
                                                                       cTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACCCCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTgg                        <  2:2678008/100‑1 (MQ=255)
                                                                       cTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTgg                        >  2:2009832/1‑100 (MQ=255)
                                                                        tGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATATACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGt                       >  1:217635/1‑100 (MQ=255)
                                                                        tGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGt                       >  1:89289/1‑100 (MQ=255)
                                                                         ggAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTg                      <  1:2354543/100‑1 (MQ=255)
                                                                         ggAAAGCCGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTg                      <  1:2079879/100‑1 (MQ=255)
                                                                              gCCGGTCGCCCAAGCTGGAAGCAACCACCACCGCCGTGCCGCTCACCACCCCATGCCAACGGCCGAACGAAGACGTGTCGATCACCCGTTAGGTGcta                   <  1:1648248/98‑3 (MQ=255)
                                                                               ccGGTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATcc                >  1:2558388/1‑100 (MQ=255)
                                                                                  gTCGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGc             <  2:1152031/100‑1 (MQ=255)
                                                                                   tCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGc             <  1:1690741/100‑1 (MQ=255)
                                                                                    cGCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTc           <  2:2350566/100‑1 (MQ=255)
                                                                                     gCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc          >  2:2280502/1‑100 (MQ=255)
                                                                                     gCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc          <  2:886238/100‑1 (MQ=255)
                                                                                     gCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc          <  2:1781721/100‑1 (MQ=255)
                                                                                     gCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc          <  2:1338803/100‑1 (MQ=255)
                                                                                     gCAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTcc          >  1:663456/1‑100 (MQ=255)
                                                                                      cAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCa         >  1:226325/1‑100 (MQ=255)
                                                                                      cAAAAGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCa         >  1:2170146/1‑100 (MQ=255)
                                                                                       aaaaGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAg        >  2:342078/1‑100 (MQ=255)
                                                                                        aaaGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGt       >  2:150154/1‑100 (MQ=255)
                                                                                        aaaGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGt       <  1:1440775/100‑1 (MQ=255)
                                                                                         aaGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGtt      <  1:830693/100‑1 (MQ=255)
                                                                                          aGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGtt      >  1:2443553/1‑100 (MQ=255)
                                                                                          aGCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTg     <  2:2724943/100‑1 (MQ=255)
                                                                                          aGCTGGAAGAAAAGACACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGc    <  2:1241075/100‑1 (MQ=255)
                                                                                           gCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTg     <  1:990516/99‑1 (MQ=255)
                                                                                           gCTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGc    <  2:519859/100‑1 (MQ=255)
                                                                                            cTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCa   >  2:463123/1‑100 (MQ=255)
                                                                                            cTGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCa   >  1:721978/1‑100 (MQ=255)
                                                                                             tGGAAGAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCAc  <  2:176532/100‑1 (MQ=255)
                                                                                                   |                                                                                              
TTCTGCTTCACCGTGGAGCCATCAATGGCGGGCAGAATAAACTTCGGTCCGGAAACGCTTTTCAACCCTTTCTGGAAAGCCGGTCGCAAAAGCTGGAAGAAAAAGACCACCGCCGTGCCGATAAACACCCACTGCCAACGGACATCCGAAGCCGTGTCGACCACCAGTTTGGTGCCATCCAGCTCCAGTTGCAC  >  NC_000913/3595212‑3595405

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: