| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 1,097,037 | (G)7→8 | intergenic (+208/+528) | ycdU → / ← serX | putative inner membrane protein/tRNA‑Ser |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,097,030 | 1 | . | G | 86.4% | 48.5 / 6.6 | 22 | intergenic (+201/+535) | ycdU/serX | putative inner membrane protein/tRNA‑Ser |
| Reads supporting (aligned to +/- strand): ref base . (1/2); new base G (5/14); total (6/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.44e-01 | |||||||||||
CCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGG‑GGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAC > NC_000913/1096932‑1097118 | ccTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑g > 1:1055167/1‑100 (MQ=255) tCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg < 1:864319/100‑1 (MQ=255) cGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑g > 1:100261/1‑100 (MQ=255) cccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑ggg > 2:2432525/1‑100 (MQ=255) ccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑gggg < 2:756283/100‑1 (MQ=255) ccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑gggg < 2:479237/100‑1 (MQ=255) ccTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑gggg < 2:226911/100‑1 (MQ=255) cTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑ggggg < 1:2751902/100‑2 (MQ=255) tAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑ggggga < 2:512043/100‑3 (MQ=255) tAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑ggggga > 2:1123300/1‑98 (MQ=255) tAACAGAACTGGGCGGGGGCACCGGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑ggggga < 1:2816415/100‑3 (MQ=17) aaCAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑gggggat < 2:2791006/100‑4 (MQ=255) aCAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑gggggatt < 2:334132/100‑5 (MQ=255) aCAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑gggggatt < 1:2084031/100‑5 (MQ=255) cAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑gggggattg > 1:13630/1‑95 (MQ=255) cAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑ggg‑gggggattg > 1:1193575/1‑95 (MQ=255) gACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGAc < 2:2612937/100‑1 (MQ=255) gACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGAc < 2:2288085/100‑1 (MQ=255) gACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTCAAATACGGCGGTGA‑ggg‑gggggaatgac > 1:517508/1‑93 (MQ=17) aCGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACt < 1:172118/100‑1 (MQ=255) cGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAACTATGGCGGTGAGGGG‑GGGGATTGACtc > 2:446643/1‑100 (MQ=255) cGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACtc < 1:1361050/100‑1 (MQ=255) cGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACtc < 2:294389/100‑1 (MQ=255) gTGGGGGGGGGCACCGGGTGTGGGCAATTAACCAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACtca < 2:13630/100‑2 (MQ=14) gTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAGAAAAAGCCCGTACTTTCGTACGAGCGCTTCTTTAAATATGGCGGTGA‑GGGTGGGGATTGACtcg < 1:1741255/100‑1 (MQ=14) gTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACtcg < 1:201930/100‑1 (MQ=255) gTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACtcg < 2:2484755/100‑1 (MQ=255) tGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACtcgc > 2:2127942/1‑100 (MQ=255) gCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACtcgcttc < 1:1114663/100‑1 (MQ=255) gCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACtcgcttc > 2:222952/1‑100 (MQ=255) gCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACccgcttc > 1:1814260/1‑100 (MQ=255) ggggCACCCGGTGTGTGCAATATACGAAAAAAAAGCACGTACTTTCGTACGAGCTCTTCTTTAAATATGACGGTGAGGGG‑GGGGATTTACtcgcttcac > 1:1333913/1‑97 (MQ=11) cACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACTCGCTTCGCTCGcc < 2:732948/100‑1 (MQ=9) cACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGG‑GGGGATTGACTCGCTTCGCTCGccc < 2:100262/100‑1 (MQ=31) cACCCGGTGCGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACTCGCTTCGCTCGcc < 2:1801173/100‑1 (MQ=255) aCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACTCGCTTCGCTCGccc < 2:2188023/100‑1 (MQ=2) aCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACTCGCTTCGCTCGccc < 2:2181783/100‑1 (MQ=2) aCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGG‑GGGGATTGACTCGCTTCGCTCGcc < 2:1568596/99‑1 (MQ=2) atGGCGGTGA‑GGG‑GGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCATGGTCTGTCCAACTGGCTGTCGCCAGTTGTctaa > 1:1867948/1‑95 (MQ=0) | CCTCGCCCTAACAGACGTGGGCGGGGGCACCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGA‑GGG‑GGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAC > NC_000913/1096932‑1097118 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |