Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 432,193 +G intergenic (‑180/+119) tsx ← / ← yajI nucleoside channel, receptor of phage T6 and colicin K/putative lipoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913432,1891.G97.6% 461.0 / 4.3 126intergenic (‑176/+123)tsx/yajInucleoside channel, receptor of phage T6 and colicin K/putative lipoprotein
Reads supporting (aligned to +/- strand):  ref base . (1/2);  new base G (54/69);  total (55/71)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-01

Only 100 of 144 total aligned reads displayed.

GAGCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCG  >  NC_000913/432092‑432283
                                                                                                   |                                                                                               
gagCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTcgggcgg                                                                                               <  2:2815421/100‑1 (MQ=255)
  gCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggg                                                                                             <  2:1212088/100‑1 (MQ=255)
  gCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggg                                                                                             >  1:2553974/1‑100 (MQ=255)
  gCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggg                                                                                             >  2:1069557/1‑100 (MQ=255)
  gCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggg                                                                                             <  1:2305531/100‑1 (MQ=255)
   ccTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCTTTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCggggg                                                                                            <  1:1571473/100‑2 (MQ=255)
    cTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCggggga                                                                                           <  1:2206036/100‑3 (MQ=255)
     tattatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggggat                                                                                          >  2:1419493/1‑97 (MQ=255)
      attatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggggatt                                                                                         <  1:2516732/100‑5 (MQ=255)
       ttatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggggattg                                                                                        <  1:1660038/100‑6 (MQ=255)
       ttatAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCgggggattg                                                                                        >  1:1024603/1‑95 (MQ=255)
         ataAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTa                                                                                      >  2:2028246/1‑100 (MQ=255)
          taAGAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTTACGTTTACATACATTCGGGCggggg                                                                                            >  1:1288589/1‑92 (MQ=255)
          taAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTa                                                                                      >  1:2538409/1‑99 (MQ=255)
          taAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTAc                                                                                     >  2:2137638/1‑100 (MQ=255)
           aaaaaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACt                                                                                    >  1:2443192/1‑100 (MQ=255)
            aaaaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTg                                                                                   >  1:382492/1‑100 (MQ=255)
              aaTCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGAc                                                                                 <  2:1340422/100‑1 (MQ=255)
                 cATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACttt                                                                              >  1:2754441/1‑100 (MQ=255)
                  aTTGCTCGCAAATATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGCATTGTACTGACTTTc                                                                             <  1:752377/100‑1 (MQ=255)
                  aTTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTc                                                                             >  1:2103214/1‑100 (MQ=255)
                  aTTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTc                                                                             >  1:1446641/1‑100 (MQ=255)
                  aTTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTc                                                                             <  2:2314879/100‑1 (MQ=255)
                    tGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGc                                                                           <  1:591764/100‑1 (MQ=255)
                     gCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGcc                                                                          >  1:434366/1‑100 (MQ=255)
                     gCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGcc                                                                          <  1:2330045/100‑1 (MQ=255)
                      cTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGccc                                                                         <  2:1569796/100‑1 (MQ=255)
                      cTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGccc                                                                         >  2:1842719/1‑100 (MQ=255)
                      cTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGccc                                                                         >  1:734041/1‑100 (MQ=255)
                       tCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCt                                                                        >  2:1043441/1‑100 (MQ=255)
                       tCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCt                                                                        >  2:1044515/1‑100 (MQ=255)
                        cacaAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGTCGGGGGATTGTACTGACTTTCGCCCtt                                                                       >  2:1038490/1‑100 (MQ=255)
                        cacaAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCtt                                                                       >  2:1038099/1‑100 (MQ=255)
                         acaAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCttg                                                                      <  1:1204544/100‑1 (MQ=255)
                         acaAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCttg                                                                      <  1:1402400/100‑1 (MQ=255)
                         acaAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCttg                                                                      <  1:2209564/100‑1 (MQ=255)
                           aaaaaTATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGAGGGGGATTGTACTGACTTTCGCCCttgtt                                                                    <  1:976846/100‑1 (MQ=255)
                           aaaaaTATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCttgtt                                                                    >  2:921958/1‑100 (MQ=255)
                            aaaaTATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCttgttg                                                                   <  2:255682/100‑1 (MQ=255)
                            aaaaTATGTTTCGTTTCACAGTTCTATCATTTACGTAACCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCttgttg                                                                   >  1:1742057/1‑100 (MQ=255)
                              aaTATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCa                                                                 <  2:1772002/100‑1 (MQ=255)
                              aaTATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCa                                                                 <  2:1524343/100‑1 (MQ=255)
                                 atGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAAc                                                              <  2:144715/100‑1 (MQ=255)
                                  tGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACt                                                             <  2:1583666/100‑1 (MQ=255)
                                    tttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACttt                                                           <  2:2787627/100‑1 (MQ=255)
                                     ttcgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACtttt                                                          <  1:1991926/100‑1 (MQ=255)
                                       cgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTAc                                                        <  1:2727605/100‑1 (MQ=255)
                                       cgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTAc                                                        <  2:2670279/100‑1 (MQ=255)
                                       cgtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTAc                                                        <  1:2630448/100‑1 (MQ=255)
                                       cgcttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTAc                                                        >  2:563157/4‑100 (MQ=255)
                                        gtttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACt                                                       <  2:2300220/100‑1 (MQ=255)
                                         tttcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTa                                                      >  1:710094/1‑100 (MQ=255)
                                           tcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGGCTTTCGCCCTTGTTGCAAACTTTTACTATc                                                    <  2:79630/100‑1 (MQ=255)
                                           tcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATc                                                    <  2:1356972/100‑1 (MQ=255)
                                           tcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATc                                                    <  2:710100/100‑1 (MQ=255)
                                           tcACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATc                                                    <  2:1451448/100‑1 (MQ=255)
                                            cacaGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCa                                                   >  1:1344741/1‑100 (MQ=255)
                                            cacaGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCa                                                   <  2:1297395/100‑1 (MQ=255)
                                            cacaGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCa                                                   >  1:165696/1‑100 (MQ=255)
                                             acaGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCaa                                                  <  2:2606344/100‑1 (MQ=255)
                                               aGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATc                                                >  1:1104393/1‑100 (MQ=255)
                                                   cTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAgg                                            <  1:2422289/100‑1 (MQ=255)
                                                     aTCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTg                                          >  1:974562/1‑100 (MQ=255)
                                                       cATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCACTCCAGGTGAt                                        >  1:1847867/1‑100 (MQ=255)
                                                        aTTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATg                                       <  2:2672992/100‑1 (MQ=255)
                                                          ttACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTa                                     <  2:1809386/100‑1 (MQ=255)
                                                           tACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTaa                                    <  1:1038483/100‑1 (MQ=255)
                                                            aCGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAg                                   >  2:2444847/1‑100 (MQ=255)
                                                              gTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCa                                 >  1:1834145/1‑100 (MQ=255)
                                                               tAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCag                                <  2:450113/100‑1 (MQ=255)
                                                                aaTCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCaga                               <  2:434371/100‑1 (MQ=255)
                                                                 aTCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCagag                              <  2:1612995/100‑1 (MQ=255)
                                                                  tCGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGt                             <  2:1999611/100‑1 (MQ=255)
                                                                   cGATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTa                            <  2:528146/100‑1 (MQ=255)
                                                                    gATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTaa                           >  1:2123709/1‑100 (MQ=255)
                                                                    gATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTaa                           >  2:716355/1‑100 (MQ=255)
                                                                    gATTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTaa                           >  2:1282048/1‑100 (MQ=255)
                                                                     aTTGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAAc                          >  2:2279385/1‑100 (MQ=255)
                                                                      ttGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACa                         <  1:1043433/100‑1 (MQ=255)
                                                                       tGCGTTCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACaa                        >  1:1519823/1‑100 (MQ=255)
                                                                           ttCACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATg                    >  2:2212639/1‑100 (MQ=255)
                                                                             cACGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATg                    >  2:516486/1‑98 (MQ=255)
                                                                              aCGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACg                 <  1:2462911/100‑1 (MQ=255)
                                                                               cGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACgg                <  2:628817/100‑1 (MQ=255)
                                                                               cGTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACgg                <  2:629002/100‑1 (MQ=255)
                                                                                gTTTACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACggg               >  1:537395/1‑100 (MQ=255)
                                                                                   tACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGaa            <  1:1842705/100‑1 (MQ=255)
                                                                                   tACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGaa            <  1:2028231/100‑1 (MQ=255)
                                                                                   tACACACATTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGaa            >  2:1747580/1‑100 (MQ=255)
                                                                                   tACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGaaa           >  1:1651152/1‑100 (MQ=255)
                                                                                     cacacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAg          >  2:2377051/1‑100 (MQ=255)
                                                                                     cacacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAg          >  1:374010/1‑100 (MQ=255)
                                                                                      acacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGa         <  1:2609262/100‑1 (MQ=255)
                                                                                       cacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGAt        <  1:672774/100‑1 (MQ=255)
                                                                                       cacaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGAt        >  2:1969115/1‑100 (MQ=255)
                                                                                        acaTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATg       <  2:1709403/100‑1 (MQ=255)
                                                                                         caTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGt      >  2:1229796/1‑100 (MQ=255)
                                                                                          aTTCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGtt     >  2:369975/1‑100 (MQ=255)
                                                                                           ttCGGGCGGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTg    <  1:1069549/100‑1 (MQ=255)
                                                                                             cgggcgggGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCg  <  2:2103229/100‑1 (MQ=255)
                                                                                                   |                                                                                               
GAGCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGTAATCGATTGCGTTCACGTTTACACACATTCGGGCGGGGATTGTACTGACTTTCGCCCTTGTTGCAAACTTTTACTATCAATCCAGGTGATGTAAGCAGAGTAACAAAATGACGGGGAAAGATGTTGCG  >  NC_000913/432092‑432283

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: