BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	715,849	A→G	L146L (TTA→TTG)	ybfP →	lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	715,849	0	A	G	99.1%	392.8 / ‑4.6	109	L146L (TTA→TTG)	ybfP	lipoprotein
Reads supporting (aligned to +/- strand): ref base A (0/1); new base G (51/57); total (51/58)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.40e-01

Only 100 of 115 total aligned reads displayed.

GCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGT  >  NC_000913/715752‑715945

gCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTgcg                                                                                                <  1:2472674/100‑1 (MQ=255)

 ccAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTgcga                                                                                               >  1:2543715/1‑100 (MQ=255)

 ccAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTgcga                                                                                               >  1:1002791/1‑100 (MQ=255)

 ccAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTgcga                                                                                               >  2:2654000/1‑100 (MQ=255)

  cAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTgcgac                                                                                              <  2:2023879/100‑1 (MQ=255)

   aGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTgcgacg                                                                                             <  1:1132762/100‑1 (MQ=255)

    gggCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGc                                                                                            >  2:1249082/1‑100 (MQ=255)

     ggCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCt                                                                                           >  1:1786957/1‑100 (MQ=255)

      gCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTg                                                                                          >  2:1240220/1‑100 (MQ=255)

          gCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAg                                                                                      >  2:2665858/1‑100 (MQ=255)

          gCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGACGCATGTCGGGATTATTGAGTTGCGACGCTGCCAg                                                                                      >  1:952540/1‑100 (MQ=255)

            ccGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAg                                                                                      >  1:1732682/1‑98 (MQ=255)

            ccGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTc                                                                                    <  1:111903/100‑1 (MQ=255)

                cACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGaa                                                                                >  2:1360698/1‑100 (MQ=255)

                 aCTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGaa                                                                                >  2:309775/1‑99 (MQ=255)

                 aCTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGaa                                                                                <  1:71885/99‑1 (MQ=255)

                  cTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGaaaa                                                                              <  2:545767/100‑1 (MQ=255)

                    cGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAAtt                                                                            >  2:425784/1‑100 (MQ=255)

                     gACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTg                                                                           <  2:426347/100‑1 (MQ=255)

                     gACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTg                                                                           <  1:2629363/100‑1 (MQ=255)

                      aCCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGc                                                                          >  1:1124898/1‑100 (MQ=255)

                         gATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAg                                                                       >  2:1600835/1‑100 (MQ=255)

                            aGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCaa                                                                    >  1:2223837/1‑100 (MQ=255)

                             gTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAg                                                                   >  2:1346545/1‑100 (MQ=255)

                             gTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAg                                                                   >  1:1357091/1‑100 (MQ=255)

                              tCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGa                                                                  >  1:1166809/1‑100 (MQ=255)

                              tCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGa                                                                  <  1:458489/100‑1 (MQ=255)

                               cAATGAGGATTATGTCAGCGAAAGCGGATTTTTTTTTTTGTTGTTGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGaa                                                                 >  1:731752/1‑100 (MQ=255)

                                  tGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTg                                                              <  2:1152317/100‑1 (MQ=255)

                                  tGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTg                                                              <  1:2705937/100‑1 (MQ=255)

                                   gAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGa                                                             <  1:1249074/100‑1 (MQ=255)

                                   gAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGa                                                             <  2:2502156/100‑1 (MQ=255)

                                    aGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTg                                                              <  2:1071375/98‑1 (MQ=255)

                                       aTTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGc                                                         <  2:1957179/100‑1 (MQ=255)

                                       aTTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGc                                                         <  1:225808/100‑1 (MQ=255)

                                         tATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGa                                                       <  2:1743538/100‑1 (MQ=255)

                                           tGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATa                                                     <  2:511915/100‑1 (MQ=255)

                                            gTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaa                                                    >  2:2143954/1‑100 (MQ=255)

                                            gTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGAGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaa                                                    <  1:1360689/100‑1 (MQ=255)

                                             tCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaaa                                                   >  1:373442/1‑100 (MQ=255)

                                             tCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATaaa                                                   >  2:1368941/1‑100 (MQ=255)

                                               aGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATa                                                 <  1:1947532/100‑1 (MQ=255)

                                               aGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATa                                                 >  2:1767300/1‑100 (MQ=255)

                                               aGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATa                                                 >  1:218166/1‑100 (MQ=255)

                                                gCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATac                                                <  2:2613948/100‑1 (MQ=255)

                                                gCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATac                                                >  1:1527097/1‑100 (MQ=255)

                                                gCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATa                                                 >  2:2553720/1‑99 (MQ=255)

                                                gCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATa                                                 >  1:659122/1‑99 (MQ=255)

                                                  gAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATacac                                              <  2:543083/100‑1 (MQ=255)

                                                  gAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATacac                                              <  2:2080469/100‑1 (MQ=255)

                                                   aaaGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATAcaca                                             <  2:1002798/100‑1 (MQ=255)

                                                      gCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTa                                          >  1:18429/1‑100 (MQ=255)

                                                      gCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTa                                          >  2:1294488/1‑100 (MQ=255)

                                                      gCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCCGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTa                                          >  2:2052491/1‑100 (MQ=255)

                                                        ggATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTaaa                                        >  1:1457877/1‑100 (MQ=255)

                                                        ggATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTaaa                                        >  2:2631096/1‑100 (MQ=255)

                                                          aTTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAAt                                      <  1:56593/100‑1 (MQ=255)

                                                          aTTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAAt                                      <  2:1785410/100‑1 (MQ=255)

                                                           ttttttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATa                                     >  1:1691206/1‑100 (MQ=255)

                                                            tttttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAg                                    <  2:367593/100‑1 (MQ=255)

                                                            tttttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAg                                    <  1:461436/100‑1 (MQ=255)

                                                            tttttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGACAGCAAGAATTGAAAGCGATAAATACACATTAAAATAg                                    <  1:664416/100‑1 (MQ=255)

                                                             ttttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAg                                    <  1:1559974/99‑1 (MQ=255)

                                                              tttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                  <  1:1842914/100‑1 (MQ=255)

                                                              tttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                  <  2:2539571/100‑1 (MQ=255)

                                                              tttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                  <  1:640391/100‑1 (MQ=255)

                                                              tttGGCTCGATGATGCATGTCGCGATTATTGTGTTGCCACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                  <  1:1902170/100‑1 (MQ=255)

                                                               ttGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTg                                  <  1:1890372/99‑1 (MQ=255)

                                                                tGGCTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAg                                <  2:2618183/100‑1 (MQ=255)

                                                                   cTCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTcc                             >  1:2610517/1‑100 (MQ=255)

                                                                    tCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTcag                            >  1:1711322/1‑98 (MQ=255)

                                                                    tCGATGATGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCg                            <  1:162475/100‑1 (MQ=255)

                                                                      gatgatGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCggg                          <  2:321939/100‑1 (MQ=255)

                                                                      gatgatGCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCggg                          <  1:1240212/100‑1 (MQ=255)

                                                                          atgCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATg                      <  2:1615104/100‑1 (MQ=255)

                                                                           tgCATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGa                     >  2:2447142/1‑100 (MQ=255)

                                                                             cATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGaaa                   >  1:2102812/1‑100 (MQ=255)

                                                                             cATGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGaaa                   >  1:594714/1‑100 (MQ=255)

                                                                              aTGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAc                  >  2:1946598/1‑100 (MQ=255)

                                                                               tGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAcc                 >  2:2287867/1‑100 (MQ=255)

                                                                               tGTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAc                  >  2:561827/1‑99 (MQ=255)

                                                                                tccgatcttattGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAccc                <  2:2703273/93‑1 (MQ=255)

                                                                                gTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAccc                <  2:273953/100‑1 (MQ=255)

                                                                                gTCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAAccc                <  2:2244913/100‑1 (MQ=255)

                                                                                 tCGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCg               >  2:440255/1‑100 (MQ=255)

                                                                                  cGGGATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCgg              <  2:1791226/100‑1 (MQ=255)

                                                                                  cGGGATTATTGAGTTGCGACGCTGCCAGGCAGAAAATTGACAGCAAGAATAGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCgg              <  1:881917/100‑1 (MQ=255)

                                                                                  cGGGATTATTGAGTTGCGACGCTGCCAGCCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCgg              <  2:363387/100‑1 (MQ=255)

                                                                                    ggATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAc            <  2:1705415/100‑1 (MQ=255)

                                                                                    ggATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAc            >  1:1929162/1‑100 (MQ=255)

                                                                                    ggATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAc            >  2:479339/1‑100 (MQ=255)

                                                                                     gATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAcc           <  1:2653977/100‑1 (MQ=255)

                                                                                     gATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAcc           <  2:1124906/100‑1 (MQ=255)

                                                                                     gATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAcc           <  1:241040/100‑1 (MQ=255)

                                                                                     gATTATTGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGAc            <  1:946027/99‑1 (MQ=255)

                                                                                       ttattGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTg         <  1:2437334/100‑1 (MQ=255)

                                                                                          ttGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAAc      >  2:2152197/1‑100 (MQ=255)

                                                                                           tGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACa     <  2:1166817/100‑1 (MQ=255)

                                                                                           tGAGTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACa     <  1:830132/100‑1 (MQ=255)

                                                                                              gTTGCGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGt  >  2:610008/1‑100 (MQ=255)

GCCAGGGCAAGCCCGGCACTCGACCGATAGTCAATGAGGATTATGTCAGCGAAAGCGGATTTTTTGGCTCGATGATGCATGTCGGGATTATTGAGTTACGACGCTGCCAGTCAGAAAATTGCCAGCAAGAATTGAAAGCGATAAATACACATTAAAATAGTGAGTCCGGGAATGAAACCCGGACCTGAACATGT  >  NC_000913/715752‑715945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 22 ≤` `ATCG/ATCG` `< 32 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`