Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,197,853 T→C N129D (AAT→GAT)  ymfE ← e14 prophage; putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,197,8530TC99.5% 677.1 / NA 183N129D (AAT→GAT) ymfEe14 prophage; putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  major base C (78/104);  minor base A (0/1);  total (78/105)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 183 total aligned reads displayed.

GAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATA  >  NC_000913/1197756‑1197949
                                                                                                 |                                                                                                
gAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGc                                                                                                <  1:1708610/100‑1 (MQ=255)
gAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGc                                                                                                >  1:1603925/1‑100 (MQ=255)
gAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGc                                                                                                >  2:1537537/1‑100 (MQ=255)
gAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGc                                                                                                <  2:1197275/100‑1 (MQ=255)
     ttCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCAtt                                                                                           >  2:980291/1‑100 (MQ=255)
     ttCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCAtt                                                                                           >  1:597419/1‑100 (MQ=255)
     ttCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCAtt                                                                                           <  1:1589449/100‑1 (MQ=255)
     ttCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCAtt                                                                                           <  1:853946/100‑1 (MQ=255)
       cAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTAc                                                                                         >  1:93071/1‑100 (MQ=255)
        aaaaaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACa                                                                                        >  2:2383306/1‑100 (MQ=255)
        aaaaaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACa                                                                                        <  1:1770462/100‑1 (MQ=255)
         aaaaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACAt                                                                                       <  1:1885854/100‑1 (MQ=255)
           aaaTATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATtg                                                                                     <  1:1318263/100‑1 (MQ=255)
             atatCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATtgtg                                                                                   <  2:1048144/100‑1 (MQ=255)
              tatCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGa                                                                                  <  1:2327232/100‑1 (MQ=255)
               atCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAg                                                                                 >  2:189421/1‑100 (MQ=255)
                tCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGc                                                                                <  2:958274/100‑1 (MQ=255)
                 ccAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCt                                                                               <  2:985465/100‑1 (MQ=255)
                  cAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAGTGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCtt                                                                              >  1:1744515/1‑100 (MQ=255)
                  cAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCtt                                                                              >  1:2620960/1‑100 (MQ=255)
                  cAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCtt                                                                              >  2:1324588/1‑100 (MQ=255)
                       aTAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCt                                                                         <  1:505525/100‑1 (MQ=255)
                        tAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtt                                                                        <  2:1611934/100‑1 (MQ=255)
                          gTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCttta                                                                      <  1:2685104/100‑1 (MQ=255)
                            ataCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttatt                                                                    <  1:1812813/100‑1 (MQ=255)
                             taCTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCtttattt                                                                   >  1:799742/1‑100 (MQ=255)
                                 ttATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAACATAGATCGCTTATTACATTGTGAGCTTCCTCTTTATTTAAtt                                                               <  2:1905159/100‑1 (MQ=255)
                                  tATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAAttt                                                              >  1:1039128/1‑100 (MQ=255)
                                    ttCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTc                                                            <  2:1606072/100‑1 (MQ=255)
                                      ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTg                                                          <  1:1074761/100‑1 (MQ=255)
                                      ccAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTg                                                          >  1:2150952/1‑100 (MQ=255)
                                       cAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGt                                                         >  2:1694899/1‑100 (MQ=255)
                                        agaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGtt                                                        <  1:1380737/100‑1 (MQ=255)
                                         gaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTg                                                       <  2:650919/100‑1 (MQ=255)
                                         gaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTg                                                       <  2:746247/100‑1 (MQ=255)
                                         gaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTg                                                       <  2:1640942/100‑1 (MQ=255)
                                         gaagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCAGCTTTATTTAATTTTCTGTTg                                                       <  1:1732406/100‑1 (MQ=255)
                                          aagaGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGa                                                      <  1:2081386/100‑1 (MQ=255)
                                           agagTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGAc                                                     >  2:2341425/1‑100 (MQ=255)
                                            gagTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACt                                                    <  1:1248158/100‑1 (MQ=255)
                                             agTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTc                                                   >  2:2548510/1‑100 (MQ=255)
                                              gTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTcc                                                  >  2:273012/1‑100 (MQ=255)
                                                tCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAg                                                >  1:361277/1‑100 (MQ=255)
                                                 cAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGc                                               <  2:1006944/100‑1 (MQ=255)
                                                 cAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGc                                               <  2:1587606/100‑1 (MQ=255)
                                                 cAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGc                                               <  1:1538467/100‑1 (MQ=255)
                                                 cAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGc                                               >  1:607540/1‑100 (MQ=255)
                                                 cAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGc                                               <  1:1688480/100‑1 (MQ=255)
                                                 cAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTCAATTTTCTGTTGACTCCAGc                                               <  1:1726733/100‑1 (MQ=255)
                                                  aaTATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGct                                              >  2:596512/1‑100 (MQ=255)
                                                      taATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTcc                                          <  2:2079371/100‑1 (MQ=255)
                                                        aTGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGt                                        <  2:1237340/100‑1 (MQ=255)
                                                        aTGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGt                                        <  1:2823179/100‑1 (MQ=255)
                                                        aTGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGt                                        <  2:2289155/100‑1 (MQ=255)
                                                         tGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTg                                       <  2:1840658/100‑1 (MQ=255)
                                                         tGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTg                                       <  2:2497807/100‑1 (MQ=255)
                                                          gTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGa                                      <  2:2592848/100‑1 (MQ=255)
                                                          gTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGa                                      >  2:2235222/1‑100 (MQ=255)
                                                          gTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGa                                      >  2:907601/1‑100 (MQ=255)
                                                            ttGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATa                                    >  1:392778/1‑100 (MQ=255)
                                                             tGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATaa                                   <  2:1058673/100‑1 (MQ=255)
                                                              gTCTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAAc                                  <  2:2023359/100‑1 (MQ=255)
                                                                cTTCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACgg                                >  2:825654/1‑100 (MQ=255)
                                                                 ttCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGt                               >  1:1508607/1‑100 (MQ=255)
                                                                  tCAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGtt                              >  1:2586116/1‑100 (MQ=255)
                                                                   cAATTTTTCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGttt                             >  2:1365946/1‑100 (MQ=255)
                                                                      tttttCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtat                          <  1:1416726/100‑1 (MQ=255)
                                                                      tttttCTTACTTCAGCGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtat                          <  2:2779821/100‑1 (MQ=255)
                                                                       ttttCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTtata                         >  1:171672/1‑100 (MQ=255)
                                                                        tttCTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATaa                        <  1:1219136/100‑1 (MQ=255)
                                                                          tCTTACTTCAGGGTAATATAGATCGCTCATTACATTATGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAAtt                      >  2:1996806/1‑100 (MQ=255)
                                                                           cTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTa                     <  2:860817/100‑1 (MQ=255)
                                                                           cTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTa                     <  2:42014/100‑1 (MQ=255)
                                                                           cTTACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTa                     <  2:2603326/100‑1 (MQ=255)
                                                                            ttACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAg                    <  1:2420204/100‑1 (MQ=255)
                                                                             tACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGa                   <  1:1021930/100‑1 (MQ=255)
                                                                             tACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGa                   >  2:2628248/1‑100 (MQ=255)
                                                                             tACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGa                   <  1:2503740/100‑1 (MQ=255)
                                                                             tACTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGa                   >  2:1123143/1‑100 (MQ=255)
                                                                              aCTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGAt                  <  2:2805942/100‑1 (MQ=255)
                                                                               cTTCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATg                 >  1:2110118/1‑100 (MQ=255)
                                                                                 tCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCt               <  1:2806073/100‑1 (MQ=255)
                                                                                 tCAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCt               <  1:2235204/100‑1 (MQ=255)
                                                                                  ctgggTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCtt              <  1:689684/98‑1 (MQ=255)
                                                                                  cAGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCtt              >  1:2138685/1‑100 (MQ=255)
                                                                                   aGGGTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTa             <  2:1300075/100‑1 (MQ=255)
                                                                                    gggTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGGTAACGGTTTTATAATTAGATGCTTAt            <  2:1726934/100‑1 (MQ=255)
                                                                                    gggTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTag            >  2:689690/1‑99 (MQ=255)
                                                                                    gggTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTAt            >  1:2310101/1‑100 (MQ=255)
                                                                                    gggTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTAt            >  1:2043951/1‑100 (MQ=255)
                                                                                    gggTAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTAt            >  1:2535360/1‑100 (MQ=255)
                                                                                      gTAATATAGATCGCTCATTACATTGTGAGGTTCATCTTTATTTAATTTAATATTAACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTAt            >  1:324244/1‑98 (MQ=255)
                                                                                       tAATATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATccc         >  1:2375943/1‑100 (MQ=255)
                                                                                        aaTATAGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCa        >  1:2652181/1‑100 (MQ=255)
                                                                                          tataGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCaaa      >  1:2285143/1‑100 (MQ=255)
                                                                                          tataGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCaaa      >  2:1951223/1‑100 (MQ=255)
                                                                                           ataGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAg     <  2:2285162/100‑1 (MQ=255)
                                                                                           ataGATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAg     <  1:1422421/100‑1 (MQ=255)
                                                                                              gATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGata  >  2:346551/1‑100 (MQ=255)
                                                                                              gATCGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGata  >  2:1818905/1‑100 (MQ=255)
                                                                                                 |                                                                                                
GAAAATTCAAAAAATATCCAAAAATAGTATACTTTATTCCAGAAGAGTTCAATATAATGTTTGTCTTCAATTTTTCTTACTTCAGGGTAATATAGATTGCTCATTACATTGTGAGCTTCATCTTTATTTAATTTTCTGTTGACTCCAGCTCTCCGTGATAACGGTTTTATAATTAGATGCTTATCCCAAAGATA  >  NC_000913/1197756‑1197949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: