Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,246,073	C→T	G435D (GGC→GAC)	treA ←	periplasmic trehalase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,246,073	0	C	T	100.0%	304.2 / NA	85	G435D (GGC→GAC)	treA	periplasmic trehalase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (42/43);  total (42/43)

GTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCC  >  NC_000913/1245976‑1246168                                                                                                  |                                                                                                gTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGgtcg                                                                                               <  1:2610272/100‑1 (MQ=255)    gTTTTGTAATCTTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGtcgccg                                                                                            <  1:753009/100‑1 (MQ=255)    gTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGtcgccg                                                                                            <  2:630826/100‑1 (MQ=255)    gTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGtcgccg                                                                                            <  2:1825522/100‑1 (MQ=255)      tttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCggg                                                                                          <  1:1595876/100‑1 (MQ=255)       ttGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGt                                                                                         <  2:1265383/100‑1 (MQ=255)        tGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGtt                                                                                        >  2:669298/1‑100 (MQ=255)         gTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTg                                                                                       >  1:2614572/1‑100 (MQ=255)          taggccTTCTGTCGCAACCCACTGTAACGGTGCCCAGCCATTTTGCGCATCCCATTGTTGCCCACGGTTCACCGACTTGGTGTTCAGTTCGCCGGGTTgc                                                                                      <  1:2207592/96‑1 (MQ=255)          tAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGTGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgc                                                                                      >  1:1398535/1‑100 (MQ=255)          tAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgc                                                                                      >  2:917102/1‑100 (MQ=255)           aaTCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgca                                                                                     <  1:338147/100‑1 (MQ=255)            ntCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcag                                                                                    >  2:2158227/2‑100 (MQ=255)             tCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcagc                                                                                   <  2:1368993/100‑1 (MQ=255)             tCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcagc                                                                                   <  2:2516499/100‑1 (MQ=255)             tCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTACCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTgcagn                                                                                   <  1:1387829/100‑2 (MQ=255)               cTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGcagcag                                                                                 <  2:1284956/100‑1 (MQ=255)               cTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGcagcag                                                                                 >  1:1062836/1‑100 (MQ=255)                 tCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAt                                                                               >  2:2353894/1‑100 (MQ=255)                 tCTGTCGCGACCCACTGTAACGGTGCCCAGCCAGTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGAGGTGTTCAGGTCGCCGGGTTGCAGCAGAt                                                                               >  1:1701431/1‑100 (MQ=255)                  cTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGAtg                                                                              >  2:2427246/1‑100 (MQ=255)                     tCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCAGTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATgtgt                                                                           <  1:1568854/100‑1 (MQ=255)                        cgACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGtttt                                                                        <  2:1748209/100‑1 (MQ=255)                          aCCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcg                                                                      <  2:316948/100‑1 (MQ=255)                          aCCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcg                                                                      >  2:2365162/1‑100 (MQ=255)                           cccACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGCTGTGTTTtcgt                                                                     <  1:2172678/100‑1 (MQ=255)                           cccACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgt                                                                     >  1:62229/1‑100 (MQ=255)                            ccACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTtcgtc                                                                    <  1:1614231/100‑1 (MQ=255)                               cTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgcc                                                                 >  1:1651581/1‑100 (MQ=255)                                 gTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgt                                                               >  2:1990524/1‑100 (MQ=255)                                  tAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtc                                                              >  1:1375359/1‑100 (MQ=255)                                  tAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgtc                                                              <  2:2692613/100‑1 (MQ=255)                                  tAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTcgtcgccgt                                                               >  1:1633016/1‑99 (MQ=255)                                     cGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCgtcgccgtcgcc                                                           <  1:2471811/100‑1 (MQ=255)                                      ggTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCa                                                          >  1:713180/1‑100 (MQ=255)                                         gCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAttt                                                       >  1:2662554/1‑100 (MQ=255)                                         gCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAttt                                                       >  1:2089896/1‑100 (MQ=255)                                         gCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTAGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAttt                                                       <  2:2735043/100‑1 (MQ=255)                                          cccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCAtttt                                                      >  1:1496188/1‑100 (MQ=255)                                           ccAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttg                                                     <  1:1299356/100‑1 (MQ=255)                                            cAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgt                                                    >  2:2456690/1‑100 (MQ=255)                                            cAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgt                                                    <  1:915562/100‑1 (MQ=255)                                            cAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgt                                                    <  2:1257040/100‑1 (MQ=255)                                            cAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgt                                                    >  1:1199686/1‑100 (MQ=255)                                              gCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTttgttg                                                  <  1:2169253/100‑1 (MQ=255)                                               ccATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTgg                                                 <  1:1084762/100‑1 (MQ=255)                                                 aTTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcg                                               >  1:1109667/1‑100 (MQ=255)                                                 aTTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcg                                               >  1:2635435/1‑100 (MQ=255)                                                 aTTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGATCAGGTAACCGGGTAGCAGAAAAAGTGTTTTCGAAGCCATCGCCATTTTGTTGgcg                                               >  2:1647453/1‑100 (MQ=255)                                                  tttGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGgcgc                                              <  2:1062844/100‑1 (MQ=255)                                                    tGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCCTTTTGTTGGCGCGa                                            >  2:117696/1‑100 (MQ=255)                                                    tGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGa                                            <  1:2427226/100‑1 (MQ=255)                                                     ggCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAt                                           <  2:1701444/100‑1 (MQ=255)                                                     ccgatctCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAt                                           <  2:2400681/94‑1 (MQ=255)                                                      gtgcATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATc                                          <  2:1398546/98‑1 (MQ=255)                                                      gcgcATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCTGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATc                                          >  1:160852/1‑100 (MQ=255)                                                      gcgcATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATc                                          <  1:2343220/100‑1 (MQ=255)                                                         cATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCttt                                       <  1:669292/100‑1 (MQ=255)                                                          aTCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTc                                      >  1:2741344/1‑100 (MQ=255)                                                           tCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCg                                     >  2:857451/1‑100 (MQ=255)                                                            cccATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGAtagatcgg                                    >  1:2400661/1‑93 (MQ=255)                                                            cccATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGc                                    <  2:1523957/100‑1 (MQ=255)                                                              cATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTg                                  <  1:1728511/100‑1 (MQ=255)                                                               aTTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGc                                 <  2:53649/100‑1 (MQ=255)                                                               aTTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGc                                 <  2:1475102/100‑1 (MQ=255)                                                                  gttgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGc                              >  1:2001492/1‑100 (MQ=255)                                                                    tgCCCACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCAt                            >  1:53157/1‑100 (MQ=255)                                                                       ccACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGa                         <  2:1109675/100‑1 (MQ=255)                                                                       ccACTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGa                         <  1:624071/100‑1 (MQ=255)                                                                         aCTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACg                       <  1:696823/100‑1 (MQ=255)                                                                         aCTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACg                       <  1:1293448/100‑1 (MQ=255)                                                                         aCTTTTCACCGACGTGGTGTTCAGGTCGCCGGCTTGCAGAAGCTGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACg                       <  1:1980652/100‑1 (MQ=255)                                                                          cTTTTCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGt                      >  1:883375/1‑100 (MQ=255)                                                                           ttttCACCGACGTGGTGTTCAGGTCGGCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGGAATTTTGTTGGCGCGATCTTTAGCTGCCGCATTGACgaa                     >  1:574553/1‑98 (MQ=255)                                                                            tttCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTAc                    >  1:1045787/1‑100 (MQ=255)                                                                             ttCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACa                   <  1:1902526/100‑1 (MQ=255)                                                                             ttCACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACa                   <  1:1902722/100‑1 (MQ=255)                                                                               cACCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTGCAGc                 >  2:861670/1‑100 (MQ=255)                                                                                aCCGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCg                >  1:2439782/1‑100 (MQ=255)                                                                                 ccGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCgn               <  1:2158212/100‑2 (MQ=255)                                                                                  cGACGTGGTGTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCggg              <  2:1651593/100‑1 (MQ=255)                                                                                        ggtgTTCAGGTCGCCGGGTTGCAGCTGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAgg        <  1:459310/100‑1 (MQ=255)                                                                                        ggtgTTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAgg        <  2:713186/100‑1 (MQ=255)                                                                                           gtTCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcg     >  1:2519870/1‑100 (MQ=255)                                                                                           gtTCAGGTCGCCGGGTTGCAGCAGATGTGCTTTCGTCGCCGTCGCCATTTTGTTGGCGCGCCCTTTCGCTGCCGCCTTGACGTACAGccgaaccag         >  1:2710337/1‑88 (MQ=255)                                                                                             tCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcggc   >  2:567448/1‑100 (MQ=255)                                                                                             tCAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGggcggc   >  1:504524/1‑100 (MQ=255)                                                                                              cAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGcc  >  1:1667764/1‑100 (MQ=255)                                                                                              cAGGTCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGcc  >  2:1379212/1‑100 (MQ=255)                                                                                                  |                                                                                                GTAGTTTTGTAATCCTTCTGTCGCGACCCACTGTAACGGTGCCCAGCCATTTGGCGCATCCCATTGTTGCCCACTTTTCACCGACGTGGTGTTCAGGCCGCCGGGTTGCAGCAGATGTGTTTTCGTCGCCGTCGCCATTTTGTTGGCGCGATCTTTCGCTGCCGCATTGACGTACAGCGGGAACAGGGCGGCC  >  NC_000913/1245976‑1246168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑