Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,411,512	G→A	A146V (GCG→GTG)	ttcA ←	tRNA s(2)C32 thioltransferase, iron sulfur cluster protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,411,512	0	G	A	100.0%	328.6 / NA	91	A146V (GCG→GTG)	ttcA	tRNA s(2)C32 thioltransferase, iron sulfur cluster protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (46/45);  total (46/45)

CTCATCAGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCT  >  NC_000913/1411419‑1411608                                                                                              |                                                                                                 cTCATCAGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATTGTGACCCAACACGATCt                                                                                            <  1:265860/100‑1 (MQ=255) cTCATCAGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCt                                                                                            >  2:1142941/1‑100 (MQ=255)   catcaGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTtc                                                                                          >  2:1514693/1‑100 (MQ=255)    atcaGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTtcg                                                                                         <  2:1990733/100‑1 (MQ=255)     tcaGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTtcgt                                                                                        <  2:2273972/100‑1 (MQ=255)      caGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTtcgtc                                                                                       >  2:2550026/1‑100 (MQ=255)       aGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTtcgtcg                                                                                      <  2:2161388/100‑1 (MQ=255)       aGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTtcgtcg                                                                                      <  2:2142903/100‑1 (MQ=255)       aGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTtcgtcg                                                                                      <  1:2576994/100‑1 (MQ=255)         tttCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGcc                                                                                    <  2:424298/100‑1 (MQ=255)         tttCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAAGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGcc                                                                                    >  2:1008741/1‑100 (MQ=255)          ttCGGTGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGccc                                                                                   <  2:1827225/100‑1 (MQ=255)          ttCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGccc                                                                                   >  2:2533784/1‑100 (MQ=255)           tCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGcccc                                                                                  <  2:2226778/100‑1 (MQ=255)            cGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGccccc                                                                                 <  1:25102/100‑1 (MQ=255)            cGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGccccc                                                                                 >  2:2669676/1‑100 (MQ=255)             ggAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCa                                                                                >  1:211656/1‑100 (MQ=255)                   aTACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCg                                                                          <  2:2097573/100‑1 (MQ=255)                    tACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGt                                                                         <  2:718783/100‑1 (MQ=255)                     aCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGtt                                                                        <  2:1214871/100‑1 (MQ=255)                     aCCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGtt                                                                        >  1:2412814/1‑100 (MQ=255)                      ccTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTg                                                                       >  2:52886/1‑100 (MQ=255)                       cTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGc                                                                      <  2:282909/100‑1 (MQ=255)                       cTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGc                                                                      <  2:1516016/100‑1 (MQ=255)                       cTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGc                                                                      <  1:2550006/100‑1 (MQ=255)                        tttCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCg                                                                     >  2:2378628/1‑100 (MQ=255)                         ttCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCgg                                                                    <  2:969787/100‑1 (MQ=255)                         ttCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCgg                                                                    <  2:750023/100‑1 (MQ=255)                         ntCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGATGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCgg                                                                    >  1:1439766/2‑100 (MQ=255)                          tCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGt                                                                   <  2:593596/100‑1 (MQ=255)                           cATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTa                                                                  <  2:2270458/100‑1 (MQ=255)                           cATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTa                                                                  >  2:1531459/1‑100 (MQ=255)                            aTCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTAc                                                                 >  1:420783/1‑100 (MQ=255)                               ttACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGAt                                                              <  2:2002034/100‑1 (MQ=255)                                tACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATa                                                             >  2:427597/1‑100 (MQ=255)                                 aCCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATaa                                                            <  1:427592/100‑1 (MQ=255)                                   cgccgTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAg                                                          <  2:2269949/100‑1 (MQ=255)                                    gcctgaGAACATATTTAAGAACAACGGTTGCCGGATATCGTCACGAGGGTGAGCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAgg                                                         <  1:1479810/95‑1 (MQ=255)                                     ccgTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGa                                                        <  1:1425780/100‑1 (MQ=255)                                      cgTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGAt                                                       <  1:1464951/100‑1 (MQ=255)                                       gTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGaca                                                      >  1:1717016/1‑98 (MQ=255)                                       gTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATa                                                      >  1:1427630/1‑100 (MQ=255)                                       gTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATa                                                      >  1:1427700/1‑100 (MQ=255)                                        tAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATAc                                                     >  2:958311/1‑100 (MQ=255)                                          gAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATTGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGCTGCGGGACGATAAAGGATAcc                                                    >  1:2633201/1‑99 (MQ=255)                                            aCATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcgcg                                                 <  1:1914910/100‑1 (MQ=255)                                            aCATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcgcg                                                 <  1:1350047/100‑1 (MQ=255)                                             cATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcga                                                >  1:1269746/1‑100 (MQ=255)                                              atatTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgac                                               <  1:1825977/100‑1 (MQ=255)                                               tatTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACcgcg                                                 >  1:2606510/1‑97 (MQ=255)                                               tatTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgacg                                              >  1:2589362/1‑100 (MQ=255)                                                atTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGcgacga                                             >  2:1333528/1‑100 (MQ=255)                                                   tAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAgg                                          >  1:66387/1‑100 (MQ=255)                                                     aGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCg                                        >  2:1959583/1‑100 (MQ=255)                                                     aGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCg                                        >  1:340373/1‑100 (MQ=255)                                                      gAACAACGTTTGCAGGATATCGTCACGATGGTGCCCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCga                                       >  1:1838876/1‑100 (MQ=255)                                                      gAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCga                                       <  1:348553/100‑1 (MQ=255)                                                      gAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCga                                       >  2:571998/1‑100 (MQ=255)                                                       aacaacGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCgag                                      <  1:52885/100‑1 (MQ=255)                                                        acaacGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCgaga                                     >  1:884400/1‑100 (MQ=255)                                                          aacGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAac                                   >  2:680299/1‑100 (MQ=255)                                                          aacGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTGGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAac                                   <  1:1514681/100‑1 (MQ=255)                                                            cGTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAacac                                 >  2:49329/1‑100 (MQ=255)                                                             gTTTGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAAcaca                                >  1:1744040/1‑100 (MQ=255)                                                                tGCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAggg                             <  2:2615743/100‑3 (MQ=255)                                                                 gCAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGa                            <  1:1264903/100‑1 (MQ=255)                                                                  cAGGATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAg                           >  1:175220/1‑100 (MQ=255)                                                                    ggATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCa                         <  2:2443963/100‑1 (MQ=255)                                                                    ggATATCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCa                         >  2:1418336/1‑100 (MQ=255)                                                                         tCGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTgg                    <  2:488210/100‑1 (MQ=255)                                                                          cGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGt                   <  1:807004/100‑1 (MQ=255)                                                                          cGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGt                   <  1:807659/100‑1 (MQ=255)                                                                          cGTCACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGt                   <  2:420788/100‑1 (MQ=255)                                                                             cACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGtttt                <  1:1142933/100‑1 (MQ=255)                                                                             cACGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGtttt                >  1:950383/1‑100 (MQ=255)                                                                              cttccgatctcccAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg               <  1:914037/90‑1 (MQ=255)                                                                              aCGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTg               >  2:350157/1‑100 (MQ=255)                                                                               cGATGGTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGc              >  2:2349801/1‑100 (MQ=255)                                                                                    gTGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCctct         >  1:2674462/1‑100 (MQ=255)                                                                                     tGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCctctn        <  2:1439778/100‑2 (MQ=255)                                                                                     tGACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTg        >  2:2189771/1‑100 (MQ=255)                                                                                      gACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTgg       <  2:1609431/100‑1 (MQ=255)                                                                                      gACCCAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTgg       <  2:66388/100‑1 (MQ=255)                                                                                        cccAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGCTAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGaa     >  2:2456627/1‑100 (MQ=255)                                                                                        cccAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTgagatcggaag     >  2:914044/1‑90 (MQ=255)                                                                                        cccAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGaa     >  2:1380556/1‑100 (MQ=255)                                                                                         ccAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAt    >  2:2596559/1‑100 (MQ=255)                                                                                         ccAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAt    >  1:28679/1‑100 (MQ=255)                                                                                         ccAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAt    >  1:2312252/1‑100 (MQ=255)                                                                                          cAACACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtc   >  2:211726/1‑100 (MQ=255)                                                                                           aaCACGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAAtct  <  2:590500/100‑1 (MQ=255)                                                                                              |                                                                                                 CTCATCAGTTTCGGAGGCATACCTTTCATCTTACCGCCGTAGAACATATTTAAGAACAACGTTTGCAGGATATCGTCACGATGGTGACCCAACGCGATCTTCGTCGCCCCCAGTTCCGTTGCGGTACGATAAAGGATACCGCGACGAAGGCGAGAACACAGTGAGCAAGTGGTTTTGCCCTCTGGAATCT  >  NC_000913/1411419‑1411608

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑