Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,903,571 A→G T164A (ACC→GCC)  manY → mannose‑specific enzyme IIC component of PTS

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,903,5710AG99.2% 460.8 / ‑4.6 125T164A (ACC→GCC) manYmannose‑specific enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (52/72);  total (53/72)
Fisher's exact test for biased strand distribution p-value = 4.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.34e-01

Only 100 of 125 total aligned reads displayed.

CAACCTGACAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGTGGTTGGTTATGCGA  >  NC_000913/1903474‑1903667
                                                                                                 |                                                                                                
cAACCTGACAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGcc                                                                                                <  1:1070169/100‑1 (MQ=255)
cAACCTGACAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGcc                                                                                                <  1:1087047/100‑1 (MQ=255)
cAACCTGACAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGcc                                                                                                <  1:579244/100‑1 (MQ=255)
cAACCTGACAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGcc                                                                                                <  1:347058/100‑1 (MQ=255)
   ccTGACAGCGATTTCCTGGATCCACGTTTCCTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGc                                                                                             <  1:1004751/100‑1 (MQ=255)
    cTGACAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCg                                                                                            <  1:2059769/100‑1 (MQ=255)
     tGACAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGa                                                                                           >  2:341678/1‑100 (MQ=255)
       aCAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAg                                                                                         <  2:298480/100‑1 (MQ=255)
       aCAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGCTGCGCTGTCTGTTGGTGCCAGCGAAg                                                                                         <  2:1720760/100‑1 (MQ=255)
        cAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGt                                                                                        >  2:2469259/1‑100 (MQ=255)
          gCGTTTTCGTGGATCCACGTTTCTTGTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCCGTTGGTGCCAGCGAAGt                                                                                        <  1:2638346/98‑1 (MQ=255)
          gCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTAc                                                                                      <  2:968621/100‑1 (MQ=255)
          gCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTAc                                                                                      >  2:1262853/1‑100 (MQ=255)
          gCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTAc                                                                                      <  1:554430/100‑1 (MQ=255)
          gCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTAc                                                                                      <  1:554096/100‑1 (MQ=255)
          gCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTAc                                                                                      >  1:201361/1‑100 (MQ=255)
           cGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACa                                                                                     >  2:1519095/1‑100 (MQ=255)
            gATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAg                                                                                    <  2:1980374/100‑1 (MQ=255)
             aTTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGa                                                                                   >  1:893091/1‑100 (MQ=255)
             aTTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGa                                                                                   <  1:183919/100‑1 (MQ=255)
             aTTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGa                                                                                   >  2:1705338/1‑100 (MQ=255)
             aTTTCCTGGATCCACGTTTCTTCTCTGTTCCGGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGa                                                                                   <  1:590758/100‑1 (MQ=255)
               ttCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAAc                                                                                 <  1:1593612/100‑1 (MQ=255)
                 ccTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACAt                                                                               >  1:1255254/1‑100 (MQ=255)
                 ccTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACAt                                                                               >  2:2135902/1‑100 (MQ=255)
                  cTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATg                                                                              >  2:1466311/1‑100 (MQ=255)
                   tGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGc                                                                             <  2:2344166/100‑1 (MQ=255)
                    ggATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCt                                                                            <  2:628156/100‑1 (MQ=255)
                    ggATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCt                                                                            <  1:1125497/100‑1 (MQ=255)
                     gATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTg                                                                           >  1:1153101/1‑100 (MQ=255)
                     gATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTg                                                                           <  2:1848944/100‑1 (MQ=255)
                       tCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGaa                                                                         >  1:856070/1‑100 (MQ=255)
                        ccACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAAt                                                                        >  1:2554180/1‑100 (MQ=255)
                         cACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATg                                                                       <  1:1385913/100‑1 (MQ=255)
                         cACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATg                                                                       <  1:1385588/100‑1 (MQ=255)
                         cACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATg                                                                       >  1:1183899/1‑100 (MQ=255)
                                cttctCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTcc                                                                >  2:2662761/1‑100 (MQ=255)
                                cttctCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTcc                                                                >  1:1113157/1‑100 (MQ=255)
                                cttctCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTcc                                                                >  2:1280485/1‑100 (MQ=255)
                                  tctcTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCgg                                                              <  2:1946576/100‑1 (MQ=255)
                                      tGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAgt                                                          >  2:1222789/1‑100 (MQ=255)
                                         tCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAgtggt                                                       <  2:154217/100‑1 (MQ=255)
                                         ctcTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAgtggt                                                       <  1:2666163/98‑1 (MQ=255)
                                           cTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAgtggtag                                                     >  2:2666186/1‑98 (MQ=255)
                                            tGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACCTGCTGAATGCGATTCCGGTAGTGGTGAc                                                    >  2:320396/1‑100 (MQ=255)
                                            tGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGAc                                                    <  2:408436/100‑1 (MQ=255)
                                            tGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGAc                                                    >  2:1124126/1‑100 (MQ=255)
                                                 gcaaTGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATg                                               <  2:1343176/100‑1 (MQ=255)
                                                  caaTGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATgg                                              >  1:351481/1‑100 (MQ=255)
                                                  caaTGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATgg                                              <  1:2147135/100‑1 (MQ=255)
                                                     tGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGCAGTACAGAACATGCTGAATGCGATTCAGGAAGTGGTCACCAATggc                                             >  1:2350189/1‑97 (MQ=255)
                                                     tGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCt                                           <  1:1871269/100‑1 (MQ=255)
                                                     tGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCt                                           <  2:281047/100‑1 (MQ=255)
                                                      gCGTGTGGCTATTCCGGCCGTCATGGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTc                                            <  2:2204483/98‑1 (MQ=255)
                                                        gtgtGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGCAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGaa                                        <  1:1647098/100‑1 (MQ=255)
                                                             gCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCg                                   <  2:1136054/100‑1 (MQ=255)
                                                             gCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCg                                   >  2:1248109/1‑100 (MQ=255)
                                                             gCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCg                                   >  2:837944/1‑100 (MQ=255)
                                                              cTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGc                                  <  1:329331/100‑1 (MQ=255)
                                                                aTTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCg                                <  1:890330/100‑1 (MQ=255)
                                                                 ttCCGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCgg                               >  2:2049572/1‑100 (MQ=255)
                                                                   ccGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTg                             <  2:416466/100‑1 (MQ=255)
                                                                   ccGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTg                             >  2:1707107/1‑100 (MQ=255)
                                                                    cGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTgg                            <  1:1509664/100‑1 (MQ=255)
                                                                    cGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTgg                            <  1:142363/100‑1 (MQ=255)
                                                                    cGGCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTgg                            >  1:1281954/1‑100 (MQ=255)
                                                                    cGGCCGTCAGCGTTGCGCGGTCTGTTGGTGCCAGCGAAGGACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCGGAATATCGCCGGTgg                            <  2:954761/100‑1 (MQ=255)
                                                                     ggCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGc                           >  1:1819940/1‑100 (MQ=255)
                                                                      gCCGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCa                          <  1:1702224/100‑1 (MQ=255)
                                                                       ccGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCAt                         >  1:2087713/1‑100 (MQ=255)
                                                                        cGTCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATg                        <  1:153085/100‑1 (MQ=255)
                                                                          tCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCgagatcggaag                      >  1:1625063/1‑90 (MQ=255)
                                                                          tCATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGAt                      >  2:492334/1‑100 (MQ=255)
                                                                           cATCGTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATc                     >  1:246248/1‑100 (MQ=255)
                                                                               gTTGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCgtgg                 >  1:1413477/1‑100 (MQ=255)
                                                                                ttGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCgtggt                <  2:2015285/100‑1 (MQ=255)
                                                                                ttGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCgtggt                <  1:1280477/100‑1 (MQ=255)
                                                                                ttGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCgtggt                <  1:341674/100‑1 (MQ=255)
                                                                                 tGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCgtggtg               <  1:1519083/100‑1 (MQ=255)
                                                                                 tGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCgtggtg               <  1:1672646/100‑1 (MQ=255)
                                                                                 tGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCgtggtg               <  2:2236404/100‑1 (MQ=255)
                                                                                 tGCGCTGTCTGTTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCgtggtg               <  2:629216/100‑1 (MQ=255)
                                                                                     ctgtctgtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGtggttg           <  2:2087728/100‑1 (MQ=255)
                                                                                     ctgtctgtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGtggttg           <  2:904472/100‑1 (MQ=255)
                                                                                       tcttgtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGtggttggt         <  1:2415323/97‑1 (MQ=255)
                                                                                       gtctgtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTggtggtagat         >  2:476044/1‑96 (MQ=255)
                                                                                       gtctgtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGtggttggt         <  2:593461/100‑1 (MQ=255)
                                                                                       ggctgtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGtggttggt         <  2:2350208/98‑1 (MQ=255)
                                                                                         ctgtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGTGGTTGGTTa       <  2:2654450/100‑1 (MQ=255)
                                                                                         ctgtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGTGGTTGGTTa       <  2:2654460/100‑1 (MQ=255)
                                                                                         ctgtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGTGGTTGGTTa       >  1:946311/1‑100 (MQ=255)
                                                                                          tgtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGtggttggtaga      >  2:2415343/1‑97 (MQ=255)
                                                                                          tgtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGTGGTTGGTTAt      >  1:1169731/1‑100 (MQ=255)
                                                                                           gtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGTGGTTGGTTATg     <  1:1262845/100‑1 (MQ=255)
                                                                                           gtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGTGGTTGGTTATg     >  1:2327022/1‑100 (MQ=255)
                                                                                           gtTGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGTGGTTGGTTATg     <  1:2241149/100‑1 (MQ=255)
                                                                                            ttGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGTGGTTGGTTATGc    <  2:1498542/100‑1 (MQ=255)
                                                                                             tGGTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGTGGTTGGTTATGCg   <  1:2717081/100‑1 (MQ=255)
                                                                                              ggTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGTGGTTGGTTATGCGa  >  2:543730/1‑100 (MQ=255)
                                                                                              ggTGCCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGTGGTTGGTTATGCGa  >  1:775482/1‑100 (MQ=255)
                                                                                                 |                                                                                                
CAACCTGACAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTGAATATCGCCGGTGGCATGATCGTGGTGGTTGGTTATGCGA  >  NC_000913/1903474‑1903667

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: