Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,731,312	G→A	intergenic (‑155/+288)	rrsG ← / ← clpB	16S ribosomal RNA of rrnG operon/protein disaggregation chaperone

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,731,312	0	G	A	100.0%	143.4 / NA	44	intergenic (‑155/+288)	rrsG/clpB	16S ribosomal RNA of rrnG operon/protein disaggregation chaperone
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (21/23);  total (21/23)

AGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACC  >  NC_000913/2731274‑2731407                                       |                                                                                                aGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTc                                    >  2:2121417/1‑100 (MQ=11)  gATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCt                                   >  1:1602136/1‑100 (MQ=14)    ttGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCttt                                 <  1:770294/100‑1 (MQ=17)    ttGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCttt                                 <  2:1264610/100‑1 (MQ=17)    ttGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCttt                                 <  2:1634772/100‑1 (MQ=17)     tGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTg                                <  2:1764227/100‑1 (MQ=18)      gTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGc                               <  2:2182956/100‑1 (MQ=25)      gTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGc                               >  2:1574082/1‑100 (MQ=25)      gTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGc                               >  1:1528842/1‑100 (MQ=25)      gTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGc                               <  1:1208858/100‑1 (MQ=25)      gTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGc                               <  2:974626/100‑1 (MQ=25)        cTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt                             >  2:53966/1‑100 (MQ=255)        cTGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtt                             >  2:1619633/1‑100 (MQ=255)         tGATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCttt                            >  1:185819/1‑100 (MQ=255)          gATAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCtttt                           >  1:2559184/1‑100 (MQ=255)           aTAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtc                          >  2:939752/1‑100 (MQ=255)            tAAATTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTTATCTGCTCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtct                         >  1:1654644/1‑100 (MQ=14)             aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCTCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                        >  1:1248286/1‑100 (MQ=255)             aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                        >  2:1130286/1‑100 (MQ=255)             aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                        >  1:1726796/1‑100 (MQ=255)             aaaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTtctc                        >  1:1726797/1‑100 (MQ=255)              aaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                       >  2:1042840/1‑100 (MQ=255)              aaTTGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTctct                       >  2:1381896/1‑100 (MQ=255)                ttGTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGt                     <  1:937738/100‑1 (MQ=255)                  gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg                   <  1:879354/100‑1 (MQ=255)                  gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg                   <  2:1260463/100‑1 (MQ=255)                  gTTAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTg                   <  1:1107816/100‑1 (MQ=255)                   ttAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                  >  2:1237402/1‑100 (MQ=255)                   ttAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTga                  >  2:961103/1‑100 (MQ=255)                    tAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgag                 <  1:1073071/100‑1 (MQ=255)                    tAAAGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgag                 <  2:151829/100‑1 (MQ=255)                     aaaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga                <  2:1558098/100‑1 (MQ=255)                     aaaGAGCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTgaga                <  1:1641630/100‑1 (MQ=255)                       agagCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGAtt              >  2:2095899/1‑100 (MQ=255)                        gagCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtc             >  2:520343/1‑100 (MQ=255)                          gCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc           <  2:2063173/100‑1 (MQ=255)                          gCAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATtctc           <  2:2631845/100‑1 (MQ=255)                           cAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa          <  1:2590788/100‑1 (MQ=255)                           cAGTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCa          <  2:217399/100‑1 (MQ=255)                             gTGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAgg        <  2:2028764/100‑1 (MQ=255)                              tGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga       <  1:2818968/100‑1 (MQ=255)                              tGCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGga       <  1:484617/100‑1 (MQ=255)                               gCCGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgan      <  1:463859/100‑2 (MQ=255)                                ccGCTTCACTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGgaga     >  1:1000245/1‑100 (MQ=255)                                   cttcacttTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAAcc  <  1:2803690/100‑1 (MQ=255)                                       |                                                                                                AGATTGTCTGATAAATTGTTAAAGAGCAGTGCCGCTTCGCTTTTTCTCAGCGGCGCGGGGTGTGCATAATACGCCTTCCCGCTACAGAGTCAAGCATTTCTTTGCTTTTCTCTGTTGAGATTCTCAGGAGAACC  >  NC_000913/2731274‑2731407

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑