Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,194,516 A→G K551K (AAA→AAG)  yqiK → PHB family membrane protein, function unknown

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,194,5160AG97.2% 372.9 / ‑2.2 107K551K (AAA→AAG) yqiKPHB family membrane protein, function unknown
Reads supporting (aligned to +/- strand):  ref base A (0/3);  new base G (60/44);  total (60/47)
Fisher's exact test for biased strand distribution p-value = 8.17e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 107 total aligned reads displayed.

GCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTT  >  NC_000913/3194420‑3194610
                                                                                                |                                                                                              
gCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCTGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACACCTCCCGTCGAAGAAAAGGc                                                                                              <  1:1246950/99‑1 (MQ=255)
 cTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCag                                                                                            >  2:401290/1‑100 (MQ=255)
 cTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCag                                                                                            <  1:138936/100‑1 (MQ=255)
   gATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCagag                                                                                          <  1:1136569/100‑1 (MQ=255)
   gATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCagag                                                                                          >  2:235414/1‑100 (MQ=255)
     ttGACTCCTTGCTCAATGAAATTGGCGTTTCAGGTGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGGAAAGGCAGAGTa                                                                                        <  1:1492495/100‑1 (MQ=255)
     ttGACTCCTTGCTCAATGAAATTGGCGATTCAGGCGGCTCACTGGCGGCCTGCACTTCACCCTTAACCTCAACAACTCCGGTCGCGGAAAGGGCagag                                                                                          >  1:75936/1‑98 (MQ=255)
      tGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTaa                                                                                       <  2:287493/100‑1 (MQ=255)
       gACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTaaa                                                                                      >  1:871578/1‑100 (MQ=255)
          tCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAAccc                                                                                   <  1:1045856/100‑1 (MQ=255)
          tCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAAccc                                                                                   <  2:731897/100‑1 (MQ=255)
           ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                  >  2:2587799/1‑100 (MQ=255)
           ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                  <  2:1643551/100‑1 (MQ=255)
           ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                  >  1:1016440/1‑100 (MQ=255)
           ccTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACAGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCct                                                                                  >  2:1994808/1‑100 (MQ=255)
              tGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCCCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCctct                                                                                <  2:111556/99‑1 (MQ=255)
               gcgcAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCctct                                                                                <  1:2272481/95‑1 (MQ=255)
                cTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTcc                                                                             <  1:2180295/100‑1 (MQ=255)
                  cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCa                                                                            >  2:2714698/1‑99 (MQ=255)
                  cAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                           >  2:110190/1‑100 (MQ=255)
                  cAATGAAATTGGCGTTTCAGGCAGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAg                                                                           >  1:1641251/1‑100 (MQ=255)
                   aaTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                          >  2:385/1‑100 (MQ=255)
                   aaTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                          >  2:1348240/1‑100 (MQ=255)
                   aaTGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAgg                                                                          >  2:1532702/1‑100 (MQ=255)
                     tGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaa                                                                        >  2:1921019/1‑100 (MQ=255)
                       aaaTTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaata                                                                      >  2:1481733/1‑100 (MQ=255)
                       aaaTTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGaata                                                                      >  2:66965/1‑100 (MQ=255)
                            ggCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATccc                                                                 <  1:1282308/100‑1 (MQ=255)
                             gCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCt                                                                >  2:2798622/1‑100 (MQ=255)
                             gCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCt                                                                <  2:1016447/100‑1 (MQ=255)
                              cGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTg                                                               <  2:2172742/100‑1 (MQ=255)
                              cGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTg                                                               >  2:2458198/1‑100 (MQ=255)
                              cGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTg                                                               <  2:1031239/100‑1 (MQ=255)
                               gTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTgg                                                              <  1:531002/100‑1 (MQ=255)
                               gTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTgg                                                              >  1:2126424/1‑100 (MQ=255)
                                tttCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGc                                                             <  2:2115862/100‑1 (MQ=255)
                                tttCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGc                                                             <  1:1190652/100‑1 (MQ=255)
                                   cAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCa                                                          >  2:714698/1‑100 (MQ=255)
                                          cTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGcc                                                   >  2:2013520/1‑100 (MQ=255)
                                          cTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGcc                                                   <  2:1411489/100‑1 (MQ=255)
                                          cTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAG‑AAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCg                                                  >  2:420323/1‑100 (MQ=255)
                                           tCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCg                                                  >  2:2067248/1‑100 (MQ=255)
                                             aCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCggg                                                <  1:1419989/100‑1 (MQ=255)
                                             aCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCggg                                                >  2:2245319/1‑100 (MQ=255)
                                             aCTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGCAAACCCTCCTCCAGGACTAATCCCTGGCCCAAAAAGCCggg                                                >  1:1403980/1‑100 (MQ=255)
                                                ggcggcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAt                                             >  1:1125010/1‑100 (MQ=255)
                                                    gcATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttt                                         <  1:739306/100‑1 (MQ=255)
                                                     cATTGACTTCACCCTTAACCTCAACACCTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                        <  1:2055907/100‑1 (MQ=255)
                                                     cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                        <  2:2622916/100‑1 (MQ=255)
                                                     cATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAtttttt                                        <  1:401286/100‑1 (MQ=255)
                                                      aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                       <  2:1100597/100‑1 (MQ=255)
                                                      aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                       <  2:1125018/100‑1 (MQ=255)
                                                      aTTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTta                                       >  2:1136582/1‑100 (MQ=255)
                                                        tGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTtata                                     <  2:2582537/100‑1 (MQ=255)
                                                        tGACTTCACCCTTAACCTCAACAACTCCCGTCGAAG‑AAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTatat                                    <  1:1364367/100‑1 (MQ=255)
                                                         gACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTatat                                    >  2:1051759/1‑100 (MQ=255)
                                                         aaCTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTatat                                    >  2:1063674/2‑100 (MQ=255)
                                                          aCTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGAttttt                                         >  1:374871/1‑94 (MQ=255)
                                                          aCTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGACCAAAAAGCCGGGGATTTTTTATATc                                   <  1:2458178/100‑1 (MQ=255)
                                                           cTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCt                                  >  1:2326210/1‑100 (MQ=255)
                                                            ttCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTg                                 <  1:235412/100‑1 (MQ=255)
                                                             tCACCCTTAACCTCAACAACTCCCGTCGTAGAAAAGGCAGAGTAAGCCCTCTTCTAGGTATTATCCCTGGCACAAAATGCCTGGGATTGTTTATATCTg                                 <  1:363990/99‑1 (MQ=255)
                                                                  cTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTcc                           >  1:1281286/1‑100 (MQ=255)
                                                                  cTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTcc                           >  1:1795281/1‑100 (MQ=255)
                                                                  cTTAACCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTcc                           >  1:2666139/1‑100 (MQ=255)
                                                                     aaCCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCt                        <  1:2798595/100‑1 (MQ=255)
                                                                      aCCTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTa                       >  2:988332/1‑100 (MQ=255)
                                                                       ccTCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaa                      >  2:39229/1‑100 (MQ=255)
                                                                         tCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaa                    >  2:1012107/1‑100 (MQ=255)
                                                                         tCAACAACTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTaaaa                    >  1:1608577/1‑100 (MQ=255)
                                                                           aacaacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAg                  <  2:871585/100‑1 (MQ=255)
                                                                           aacaacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAg                  <  1:714486/100‑1 (MQ=255)
                                                                           aacaacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAg                  <  2:2698123/100‑1 (MQ=255)
                                                                           aacaacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAg                  >  1:1068766/1‑100 (MQ=255)
                                                                             caacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTg                >  2:619775/1‑100 (MQ=255)
                                                                              aacTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGc               >  1:92152/1‑100 (MQ=255)
                                                                               acTCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCa              <  2:2270698/100‑1 (MQ=255)
                                                                                 tCCCGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCaaa            >  2:220601/1‑100 (MQ=255)
                                                                                  cccGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAAt           >  1:2371338/1‑100 (MQ=255)
                                                                                  cccGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAAt           >  2:1923669/1‑100 (MQ=255)
                                                                                  cccGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAAAAAAAAAAAAAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGc               <  1:503303/96‑1 (MQ=18)
                                                                                   ccGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg          >  2:2412854/1‑100 (MQ=255)
                                                                                   ccGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg          >  1:388575/1‑100 (MQ=255)
                                                                                   ccGTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg          <  1:1614253/100‑1 (MQ=255)
                                                                                   ccGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATg          <  2:721989/100‑1 (MQ=255)
                                                                                     gTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGAt        >  2:1257247/1‑100 (MQ=255)
                                                                                     gTCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGAAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGAt        >  1:1852081/1‑100 (MQ=255)
                                                                                      tCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc       <  2:2666162/100‑1 (MQ=255)
                                                                                      tCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc       <  1:66964/100‑1 (MQ=255)
                                                                                      tCGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc       <  1:1481721/100‑1 (MQ=255)
                                                                                       cGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATc       >  2:996707/1‑99 (MQ=255)
                                                                                       cGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa      >  2:2820460/1‑100 (MQ=255)
                                                                                       cGAAGAAAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCa      >  1:1660350/1‑100 (MQ=255)
                                                                                        gaagaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAg     >  2:1677616/1‑100 (MQ=255)
                                                                                         aagaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAgg    <  2:2326229/100‑1 (MQ=255)
                                                                                         aagaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAgg    >  2:1010133/1‑100 (MQ=255)
                                                                                          agaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt   >  2:2507302/1‑100 (MQ=255)
                                                                                          agaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt   >  2:2425964/1‑100 (MQ=255)
                                                                                          agaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgt   >  2:1860685/1‑100 (MQ=255)
                                                                                           gaaAAGGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGgtt  >  2:859305/1‑100 (MQ=255)
                                                                                                |                                                                                              
GCTGATTGACTCCTTGCTCAATGAAATTGGCGTTTCAGGCGGCTCACTGGCGGCATTGACTTCACCCTTAACCTCAACAACTCCCGTCGAAGAAAAAGCAGAGTAAACCCTCTTCCAGGAATAATCCCTGGCCCAAAAAGCCGGGGATTTTTTATATCTGCGTTCCGCTAAAAAGTGCAAATGATCAGGTT  >  NC_000913/3194420‑3194610

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑