Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,572,318 T→C E395E (GAA→GAG)  glgB ← 1,4‑alpha‑glucan branching enzyme

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,572,3180TC99.0% 347.6 / ‑4.7 96E395E (GAA→GAG) glgB1,4‑alpha‑glucan branching enzyme
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base C (50/45);  total (50/46)
Fisher's exact test for biased strand distribution p-value = 4.79e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.86e-01

CAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCCTGATGATAGCCTTC  >  NC_000913/3572221‑3572413
                                                                                                 |                                                                                               
cAAATTCGTTCGGGATCCACTCCCCCTCTTTACGCCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATGCCAAAACGTTc                                                                                               <  1:1489214/100‑1 (MQ=255)
  aaTTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCaa                                                                                             <  2:2803536/100‑1 (MQ=255)
   aTTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAAt                                                                                            <  2:247146/100‑1 (MQ=255)
      cgttcgGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCa                                                                                         >  1:520226/1‑100 (MQ=255)
       gttcgGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAg                                                                                        >  1:2712877/1‑100 (MQ=255)
       gttcgGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAg                                                                                        >  2:59311/1‑100 (MQ=255)
       gttcgGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAg                                                                                        <  2:489530/100‑1 (MQ=255)
       gttcgGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAg                                                                                        >  2:445412/1‑100 (MQ=255)
        ttcgGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGt                                                                                       <  1:1109635/100‑1 (MQ=255)
        ttcgGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGt                                                                                       <  1:2733179/100‑1 (MQ=255)
        ttcgGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGt                                                                                       <  2:1727539/100‑1 (MQ=255)
          cgGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTaa                                                                                     >  1:136489/1‑100 (MQ=255)
          cgGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTaa                                                                                     >  1:672260/1‑100 (MQ=255)
          cgGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTaa                                                                                     >  1:946969/1‑100 (MQ=255)
          cgGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTaa                                                                                     >  1:134933/1‑100 (MQ=255)
           gggATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTaaa                                                                                    >  1:2691849/1‑100 (MQ=255)
            ggATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAg                                                                                   >  1:1187278/1‑100 (MQ=255)
              aTCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAgcg                                                                                 >  2:817780/1‑100 (MQ=255)
                ccACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGcgcg                                                                               <  2:28463/100‑1 (MQ=255)
                 cACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGCCGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGt                                                                              >  2:974458/1‑100 (MQ=255)
                   cTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGt                                                                              <  1:805228/98‑1 (MQ=255)
                   cTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTa                                                                            >  1:436518/1‑100 (MQ=255)
                     cccccTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTAcc                                                                          >  2:972984/1‑100 (MQ=255)
                     cccccTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTAcc                                                                          <  1:2629964/100‑1 (MQ=255)
                     cccccTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTAcc                                                                          <  1:2630137/100‑1 (MQ=255)
                       cccTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACcga                                                                        >  2:1474361/1‑100 (MQ=255)
                         ctctTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACcgacg                                                                      <  2:1115729/100‑1 (MQ=255)
                             ttACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGa                                                                  <  1:724819/100‑1 (MQ=255)
                                 ggCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGtn                                                              <  1:2099518/100‑2 (MQ=255)
                                   cTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTAc                                                            <  1:673646/100‑1 (MQ=255)
                                    tGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACt                                                           <  1:439538/100‑1 (MQ=255)
                                     tcttccgatctAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTg                                                          <  2:943566/90‑1 (MQ=255)
                                     gTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTg                                                          >  1:2766045/1‑100 (MQ=255)
                                     gTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTg                                                          <  2:436523/100‑1 (MQ=255)
                                       aGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGAc                                                        <  2:727114/100‑1 (MQ=255)
                                       aGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGAc                                                        <  1:1348858/100‑1 (MQ=255)
                                       aGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGAc                                                        >  2:413377/1‑100 (MQ=255)
                                        gTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACt                                                       <  1:840506/100‑1 (MQ=255)
                                        gTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACt                                                       >  2:2755849/1‑100 (MQ=255)
                                          cgcgATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTc                                                     >  2:291572/1‑100 (MQ=255)
                                             gATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACg                                                  >  1:453947/1‑100 (MQ=255)
                                                aaaTCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGagatcggaaga                                               >  1:943559/1‑90 (MQ=255)
                                                  aTCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGa                                              >  2:1931625/1‑99 (MQ=255)
                                                  aTCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGAc                                             >  2:1675332/1‑100 (MQ=255)
                                                  aTCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGAc                                             >  2:1129445/1‑100 (MQ=255)
                                                   tCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGAcc                                            <  2:2480401/100‑1 (MQ=255)
                                                    cATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCa                                           >  1:1080234/1‑100 (MQ=255)
                                                     aTTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCAt                                          >  1:2120171/1‑100 (MQ=255)
                                                      tagACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATa                                         <  1:1711612/98‑1 (MQ=255)
                                                       cttccgatctgCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAg                                        <  2:1315671/90‑1 (MQ=255)
                                                        gACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAg                                        >  2:1732250/1‑99 (MQ=255)
                                                        gACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGt                                       >  2:1711500/1‑100 (MQ=255)
                                                        gACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGt                                       >  2:2125691/1‑100 (MQ=255)
                                                        gACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGt                                       <  2:79526/100‑1 (MQ=255)
                                                         aCGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGtt                                      <  1:1212103/100‑1 (MQ=255)
                                                            ccACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTa                                   <  1:627153/100‑1 (MQ=255)
                                                            ccACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTa                                   <  2:1481770/100‑1 (MQ=255)
                                                            ccACCGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTa                                   <  1:1451934/100‑1 (MQ=255)
                                                                cGCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATc                               <  1:1798124/100‑1 (MQ=255)
                                                                 gCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAgagatcggaag                              >  1:1315663/1‑90 (MQ=255)
                                                                 gCATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCa                              <  1:1784345/100‑1 (MQ=255)
                                                                  cATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAg                             <  2:2712901/100‑1 (MQ=255)
                                                                  cATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAg                             <  1:1222838/100‑1 (MQ=255)
                                                                  cATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAg                             >  1:2573487/1‑100 (MQ=255)
                                                                  cATCGACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAg                             >  2:2020617/1‑100 (MQ=255)
                                                                      gaggcgcagcgcaTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGGTACTGACTTCACGGCGACCATAGGTGTAGATCAGCgtg                         >  2:1272055/4‑100 (MQ=255)
                                                                      gACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCgtg                         >  2:2267389/1‑100 (MQ=255)
                                                                      gACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCgtg                         >  2:2738574/1‑100 (MQ=255)
                                                                      gACGCGCAGCGCATCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCgtg                         >  2:2389448/1‑100 (MQ=255)
                                                                          ttccgatcttCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTcc                     <  2:2256145/91‑1 (MQ=255)
                                                                          cgcagcgcaTCACGACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTcc                     <  2:779481/100‑1 (MQ=255)
                                                                          cgcagcgcaTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTcc                     <  2:2451151/100‑1 (MQ=255)
                                                                           gcagcgcaTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCa                    >  1:572738/1‑100 (MQ=255)
                                                                           gcagcgcaTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCa                    >  2:263618/1‑100 (MQ=255)
                                                                            cagcgcaTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAg                   <  1:2755824/100‑1 (MQ=255)
                                                                            cagcgcaTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAg                   >  1:1063964/1‑100 (MQ=255)
                                                                             agcgcaTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGt                  >  2:233704/1‑100 (MQ=255)
                                                                              gcgcaTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTc                 >  2:1135735/1‑100 (MQ=255)
                                                                               cgcaTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTcc                <  1:817774/100‑1 (MQ=255)
                                                                               cgcaTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTcc                <  2:965167/100‑1 (MQ=255)
                                                                               cgcaTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTcc                >  2:1348116/1‑100 (MQ=255)
                                                                               cgcaTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTcc                <  1:605712/100‑1 (MQ=255)
                                                                                  aTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTTACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCCtga             >  2:1632719/1‑100 (MQ=255)
                                                                                  aTCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCCtga             <  1:1474349/100‑1 (MQ=255)
                                                                                   tCAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAgatcggaa            >  1:2256127/1‑93 (MQ=255)
                                                                                    cAATACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCCtgatg           <  2:637948/100‑1 (MQ=255)
                                                                                      aTACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCCTgatgat         <  2:2573508/100‑1 (MQ=255)
                                                                                       tACCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCCTGATGATa        >  1:1444737/1‑100 (MQ=255)
                                                                                        aCCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCCTGATGATAg       >  2:2140764/1‑100 (MQ=255)
                                                                                        aCCAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCCTGATGATAg       >  2:120709/1‑100 (MQ=255)
                                                                                         ccAAAACGCTCAATCCAGTAACGCGCGGTACCGACGAGGGAGTTCCTGCCTCCGCGGCGCCCAACGTTGTAGATCAGCGTGTTCCAGTCCTGataata        >  1:2158377/1‑98 (MQ=255)
                                                                                         ccAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCCTGATGATAGc      >  1:1906166/1‑100 (MQ=255)
                                                                                         ccAAAACGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCCTGATGATAGc      <  1:1711487/100‑1 (MQ=255)
                                                                                           acaaCGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCATCGTGTTCCAGTCCTGATGATAGcc     <  1:974451/97‑1 (MQ=255)
                                                                                           aaaaCGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCCTGATGATAGCCt    >  2:2781818/1‑100 (MQ=255)
                                                                                             aaCGCTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCCTGATGATAGCCTTc  <  1:1219516/100‑1 (MQ=255)
                                                                                                 |                                                                                               
CAAATTCGTTCGGGATCCACTCCCCCTCTTTACGGCTGTAGTCGCGATAAATCATTGACGCCACCGCATCGACGCGCAGCGCATCAATACCAAAACGTTCAATCCAGTAAAGCGCGTTACCGACGAGGAAGTTACTGACTTCACGGCGACCATAGTTGTAGATCAGCGTGTTCCAGTCCTGATGATAGCCTTC  >  NC_000913/3572221‑3572413

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑