Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 4,410,053 (T)9→8 100% intergenic (+47/‑80) rlmB → / → yjfI 23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,410,0450T.88.1% 317.8 / 35.9 92intergenic (+39/‑88)rlmB/yjfI23S rRNA mG2251 2'‑O‑ribose methyltransferase, SAM‑dependent/DUF2170 family protein
Reads supporting (aligned to +/- strand):  ref base T (6/3);  new base . (42/39);  total (49/43)
Fisher's exact test for biased strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.59e-01

Only 100 of 115 total aligned reads displayed.

CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATG  >  NC_000913/4409946‑4410140
                                                                                                   |                                                                                               
ccTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGt                                                                                                 >  1:634252/1‑100 (MQ=255)
 cTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtt                                                                                                >  1:226353/1‑100 (MQ=255)
  tGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtt                                                                                                <  2:1662720/99‑1 (MQ=255)
   gAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttt                                                                                              >  1:2045044/1‑100 (MQ=255)
    aaCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGAGGTGCGCCAGCGCAGCTAAGTTCTAAAAAAAAAGAAAAACCATCCAAATCTGGATGGcttt                                                                                              <  2:254471/99‑1 (MQ=25)
     aCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttt                                                                                            >  2:1151787/1‑100 (MQ=255)
     aCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttt                                                                                            <  2:580828/100‑1 (MQ=255)
     aCGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttt                                                                                            <  1:1355822/100‑1 (MQ=255)
      cGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttt                                                                                           <  2:172723/100‑1 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGtttttttt                                                                                          <  2:893121/100‑1 (MQ=255)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcat                                                                                          <  1:860113/100‑4 (MQ=37)
       gTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTGCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcat                                                                                          <  2:676136/100‑4 (MQ=37)
        tttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttg                                                                                         <  1:1484821/100‑2 (MQ=255)
        tttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcata                                                                                         <  2:35025/100‑5 (MQ=37)
         ttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgt                                                                                        >  1:1253303/1‑98 (MQ=255)
         ttCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTg                                                                                        <  2:2293051/100‑1 (MQ=255)
         ttCGGGTGCGACCGGAATTTTCTTATTTTAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAACCATCCAAATCTGGATGGcttttcataa                                                                                        <  2:1382323/100‑6 (MQ=25)
          tCGGTTGCTTCCGGAATTTGATTATTTTAAGCGGTGCACCAGCGCAGCTAATTTCTCAGAATTATGAATAACCATCCAGATCTGGTTGGcttttcataat                                                                                       <  2:844725/100‑7 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCATAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgt                                                                                        >  2:2312766/1‑97 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                       >  2:1168672/1‑97 (MQ=255)
          tCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGttttttttgtc                                                                                       <  2:457118/100‑4 (MQ=255)
            ggTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCta                                                                                    >  2:1271679/1‑100 (MQ=255)
             gTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATCAAAAGGCATCCAGAGTTGGATGGTTTTTTTTGTCtat                                                                                   >  2:1605999/1‑100 (MQ=255)
              ttGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCtata                                                                                  <  2:290787/100‑1 (MQ=255)
                 cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCt                                                                               >  2:668000/1‑100 (MQ=255)
                 cGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCt                                                                               <  1:985083/100‑1 (MQ=255)
                 cGACCGGAATTTGCTTATTTAAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGACATCCAGATATGGATGGgtttttttgtatata                                                                                   >  2:1507519/1‑90 (MQ=255)
                   aCCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTgg                                                                             >  1:1100810/1‑100 (MQ=255)
                   aCCGGAATTTGCTTATTTGAAGCGGTGAGCAAGCGCAGCTAATTTCTCAGAATGATGAAAAGTAATCCAGATTTGGATGGATTTTTTTGTCTATAg                                                                                 >  2:495597/1‑96 (MQ=255)
                    ccGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTgg                                                                             <  1:799684/99‑1 (MQ=255)
                    ccGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGt                                                                            >  2:1504648/1‑100 (MQ=255)
                      ggAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTaa                                                                          >  1:1974670/1‑100 (MQ=255)
                      ggAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTaa                                                                          >  1:1992822/1‑100 (MQ=255)
                          tttGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATa                                                                      <  1:1168678/100‑1 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                                  >  2:2211537/1‑100 (MQ=255)
                              cTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTa                                                                  >  1:1718171/1‑100 (MQ=255)
                               ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTAc                                                                 <  1:1663197/100‑1 (MQ=255)
                               ttATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTAc                                                                 <  1:893956/100‑1 (MQ=255)
                               gtATTTGAAGCGGTGCGCCAGCACAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAAtt                                                                   <  2:2174188/97‑1 (MQ=255)
                                tATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACg                                                                <  1:1169838/100‑1 (MQ=255)
                                  tttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAGTTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTa                                                              >  1:1684334/1‑100 (MQ=255)
                                  tttGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTa                                                              <  1:198423/100‑1 (MQ=255)
                                    tGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTAtt                                                            <  1:1332023/100‑1 (MQ=255)
                                    tGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTAtt                                                            >  2:549388/1‑100 (MQ=255)
                                     gAAGCGTTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTg                                                           <  2:828481/100‑1 (MQ=255)
                                     gAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTg                                                           >  1:1014512/1‑100 (MQ=255)
                                       aGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCa                                                         >  1:967010/1‑100 (MQ=255)
                                        gCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCaa                                                        >  2:1199554/1‑100 (MQ=255)
                                            tgagccacagcAGCTATGTTCTCAGAATTATTGATAGGCATTCAGATTTGGATGGTTTTTTTTGTTTATAGCTGGGAAGATAATTACGTATTGCAAat                                                      <  2:2013830/89‑1 (MQ=255)
                                               gcCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAata                                                     <  2:1376468/96‑1 (MQ=255)
                                                ccAGCGCAGTTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCa                                                 <  1:1199020/100‑1 (MQ=255)
                                                ccAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTTTTATTGTTGGTAAGATAATTACGTATTGCAAATATTCAt                                                >  2:88258/1‑100 (MQ=255)
                                                ccAGCGCAGCTAATTTCGCAGAAATATGAAAAGCCATCCAGATTTGGATGGTTTTGATTGATTATAGCTAGTAACATAAGTACGTACTGCAAATAttaa                                                 >  2:571029/1‑97 (MQ=255)
                                                 cAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtt                                               >  1:1876205/1‑100 (MQ=255)
                                                 cAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtt                                               <  1:626750/100‑1 (MQ=255)
                                                 cAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtt                                               >  1:1259012/1‑100 (MQ=255)
                                                  aGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAttt                                              <  1:1371090/100‑1 (MQ=255)
                                                   gcgcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtttt                                             <  1:1212293/100‑1 (MQ=255)
                                                     tcAGTTAATTTCTATTAATTATGAAAAGATATCCAGATTTGGTTAGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCAtt                                               <  2:1868248/95‑1 (MQ=255)
                                                     gcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTTCTATATTTAGAAAGATAGTTACATATTGTGAATTTTccattt                                             >  2:1881741/1‑93 (MQ=255)
                                                     gcAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTttat                                           <  1:2008624/100‑1 (MQ=255)
                                                      cAGCTAATTTCTCAGAATTATGAAAAGCCATCCACATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATCTTCGTTttatt                                          <  2:1253296/100‑1 (MQ=255)
                                                        gCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATCATTACGTATTGCAAATATTCATTTTATTaa                                        >  2:1919516/1‑100 (MQ=255)
                                                        gCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTaa                                        <  1:848600/100‑1 (MQ=255)
                                                         cTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAat                                       <  1:1806056/100‑1 (MQ=255)
                                                             tttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAttt                                   >  1:2202094/1‑100 (MQ=255)
                                                              ttCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTa                                  >  2:214729/1‑100 (MQ=255)
                                                               tctcAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGTTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATAttt                                   >  2:503817/1‑98 (MQ=255)
                                                               tctcAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTa                                  >  2:621792/1‑99 (MQ=255)
                                                                    gAATTATGAAAAGTCATTCAGATTTAGTTGGTTTTTTTTGTCTATAACTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAAc                               <  2:1787197/97‑1 (MQ=255)
                                                                    gAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACtt                             >  1:1262152/1‑100 (MQ=255)
                                                                    gAATTATGAAAAGCCATCCAGATTAGGATGGTTTTTTTTGTCTATAGCTGATAAGATAATTACGTATTGCAAATATTGATTTTATTAATATTTtaaat                              >  2:1395851/1‑96 (MQ=255)
                                                                      gacaaaaaaaACCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACTTATTTCAAATATTCATTTTATTAATATTTAAACTTAAt                          >  2:1476558/7‑100 (MQ=37)
                                                                      aTTATGCAAAGTCATCCAGAATTTTATGCTTTTTTTTATCTATAGTTGGTAATAGAATTACTTTTTACAAATATTAATTTTATTAATATTTAAACTTAAt                          <  2:1326627/100‑1 (MQ=255)
                                                                      aTTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAAt                          <  2:1943842/100‑1 (MQ=255)
                                                                       ttATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATc                         <  1:214730/100‑1 (MQ=255)
                                                                        tATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                        >  1:90566/1‑100 (MQ=255)
                                                                        caaaaaaaACCATCCAAATCTGGATGGCTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCt                        >  2:1218288/5‑100 (MQ=25)
                                                                          tGAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGATGGTAAGATAATTACGTATTGCAAATATTCATTTTATGAATAGTTAAACTTAATCta                       >  2:1186409/1‑99 (MQ=255)
                                                                           gAAAAGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGATGGTAAGATAATTACGTATTGCAAATATTCATTTTTTTAATATTTAAACTTAATCtat                      >  2:569891/1‑99 (MQ=255)
                                                                            aaaaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtattt                    <  1:1605270/100‑3 (MQ=255)
                                                                            aaaaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTatat                    >  1:1739541/1‑100 (MQ=255)
                                                                            aaaaACCATCCAAATCTGGATGGCTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCtata                     >  2:577229/1‑100 (MQ=38)
                                                                             aaaaCCATCCAAATCTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATg                   >  1:183646/1‑100 (MQ=255)
                                                                             aaaGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGTTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATg                   <  2:534473/100‑1 (MQ=255)
                                                                               aGCCATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTTGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGa                 <  2:1285885/100‑1 (MQ=255)
                                                                                  cATCCAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaata              >  1:2303150/1‑100 (MQ=255)
                                                                                   aTCCAGATTTGGATGGTTTTTTTTGTTTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGaataa             >  1:859991/1‑100 (MQ=255)
                                                                                      cAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatat          <  1:1191262/100‑1 (MQ=255)
                                                                                      cAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatat          <  2:610314/100‑1 (MQ=255)
                                                                                      cAGATTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatat          >  1:394260/1‑100 (MQ=255)
                                                                                        gATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatata         <  2:204095/100‑1 (MQ=255)
                                                                                        gATTTGGATGGTTTTTTTTTTTTTTTTTTTGTAAGAAAATTACCTATTTCAATTATTCATTTTATTAATTTTTAAACTTAATATATATGGAATAatatat        >  2:179377/1‑100 (MQ=255)
                                                                                         aTTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatatat        >  2:1848337/1‑99 (MQ=255)
                                                                                         aTTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATg       <  2:2082461/100‑1 (MQ=255)
                                                                                         aTTTGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATg       >  1:2126391/1‑100 (MQ=255)
                                                                                          tttGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAatata         >  2:2222957/1‑97 (MQ=255)
                                                                                          tttGGATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGa      <  2:1100805/100‑1 (MQ=255)
                                                                                          tttGGATGGTTTTTTTTGTCTAGAGCTTGTAAGATAATTACGTATTGCAAATAGTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATg       <  2:181338/99‑1 (MQ=255)
                                                                                              gATGGTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATg  <  1:1151793/100‑1 (MQ=255)
                                                                                                   |                                                                                               
CCTGAACGTTTCGGTTGCGACCGGAATTTGCTTATTTGAAGCGGTGCGCCAGCGCAGCTAATTTCTCAGAATTATGAAAAGCCATCCAGATTTGGATGGTTTTTTTTTGTCTATAGCTGGTAAGATAATTACGTATTGCAAATATTCATTTTATTAATATTTAAACTTAATCTATATGGAATAATATATGACATG  >  NC_000913/4409946‑4410140

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: