Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,314,682 Δ1 bp 15.2% intergenic (+24/+36) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,314,6810A.15.2% 253.0 / 43.7 92intergenic (+23/+37)ompW/yciEouter membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (42/34);  new base . (8/6);  total (50/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.06e-02

GGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGG  >  NC_000913/1314583‑1314780
                                                                                                  |                                                                                                    
gggCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTat                                                                                                     <  1:742882/100‑2 (MQ=255)
gggCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTaa                                                                                                     >  1:997819/1‑100 (MQ=255)
 ggCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTGTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAat                                                                                                    <  2:1791511/100‑1 (MQ=255)
   cGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAatat                                                                                                 <  1:1703182/100‑1 (MQ=255)
       ggagaggaaCGCGATAGCGTACGCCGCGAGCCGTGGGTGGGTATGTTCGCAGCAGGATATCGTTTTTGATTCCGCACAAAAACGACCCCGTAATATACgg                                                                                             <  2:2239815/93‑1 (MQ=255)
        cacaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACggg                                                                                            <  1:784685/100‑1 (MQ=255)
         acaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgggg                                                                                           <  2:2054070/100‑1 (MQ=255)
         acaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACggg                                                                                            <  1:389149/99‑1 (MQ=255)
          caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                          >  2:523625/1‑100 (MQ=255)
          caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                          <  1:1128457/100‑1 (MQ=255)
          caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                          >  1:2245037/1‑100 (MQ=255)
          caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGt                                                                                          >  1:303687/1‑100 (MQ=255)
           aGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTc                                                                                         <  1:1039255/100‑1 (MQ=255)
            gCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgggg                                                                                           >  2:185143/1‑97 (MQ=255)
            gCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcg                                                                                        <  2:303685/100‑2 (MQ=255)
            gCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcg                                                                                        <  2:1838060/100‑2 (MQ=255)
              aaCACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGCACCCGTAATATACGGGGTCAAt                                                                                      >  2:460272/1‑100 (MQ=255)
              aaCACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcgtt                                                                                      <  2:1495628/100‑4 (MQ=255)
              aaCACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcgtt                                                                                      <  1:772618/100‑4 (MQ=255)
                 acGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAg                                                                                   <  1:830445/100‑1 (MQ=255)
                 acGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAg                                                                                   >  2:1130107/1‑100 (MQ=255)
                 acGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGATCAATAAg                                                                                   <  1:472348/100‑1 (MQ=255)
                  cGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAgg                                                                                  >  1:1339949/1‑100 (MQ=255)
                  cGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAgg                                                                                  <  2:1874243/100‑1 (MQ=255)
                  cGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcgtttttg                                                                                  <  1:885607/100‑8 (MQ=38)
                  cGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGAAGGATATCGTTTTTAATTCAGCACAAAAACGACCCCGTAATATACGGGGGAAATaa                                                                                    >  1:38381/1‑98 (MQ=255)
                   gttagcccaCGCCTCGATCCCTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGa                                                                                 <  2:1613930/92‑1 (MQ=255)
                    aTAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcgtttttgtg                                                                                <  1:2360256/100‑10 (MQ=37)
                       gCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATg                                                                             <  2:18921/100‑1 (MQ=255)
                        cGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                                            <  1:44725/100‑1 (MQ=255)
                         gTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGt                                                                           >  2:93845/1‑100 (MQ=255)
                          tACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGta                                                                          <  2:18384/100‑1 (MQ=255)
                             gCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGAACAAAAACGACCACGTAATGTACGGGGTCAATAAGGAAATGGTATaa                                                                       >  2:1943015/1‑100 (MQ=255)
                              ccTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaaa                                                                      >  1:1668882/1‑100 (MQ=255)
                              ccTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaaa                                                                      >  2:950564/1‑100 (MQ=255)
                              ccTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaaa                                                                      >  1:2312858/1‑100 (MQ=255)
                              ccTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaaa                                                                      >  2:1855218/1‑100 (MQ=255)
                               cTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTAAATACCGCACAAAAACGATCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAg                                                                     >  2:1294103/1‑100 (MQ=255)
                                      cGTGGGTGTTTATGTTCTCAGCAGGGTATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatta                                                              >  1:871416/1‑100 (MQ=255)
                                      cGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatta                                                              >  1:471991/1‑100 (MQ=255)
                                      cGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatta                                                              >  1:505923/1‑100 (MQ=255)
                                      cGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTACAAAGGGtatta                                                              >  1:2267788/1‑100 (MQ=255)
                                       gTGGGTGTTTATGTTCTCAGGAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtat                                                                <  2:1954691/97‑1 (MQ=255)
                                         gggTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTAttt                                                           >  1:1834483/1‑100 (MQ=255)
                                         gggTGTGTATATTCTCAACAGGATATCGTTTTTAATTCCGCACAAAAACGACCCAGTAATATACGGGGTCAATAAGGACATCGTAGAAAAGCtatt                                                               >  2:3667/1‑96 (MQ=255)
                                          ggTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttc                                                          >  1:840766/1‑100 (MQ=255)
                                             gtTTATGGTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTAAATAAGGACATGGTATAAAGGGTATTATttcttc                                                       <  1:1339926/100‑1 (MQ=255)
                                                tATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCt                                                    >  1:376717/1‑100 (MQ=255)
                                                tATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCt                                                    >  1:1853901/1‑100 (MQ=255)
                                                 aTGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCtt                                                   <  2:1820282/100‑1 (MQ=255)
                                                     tctcAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTAc                                               <  1:1282892/100‑1 (MQ=255)
                                                      ctcAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACg                                              >  1:913641/1‑100 (MQ=255)
                                                        cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGc                                             >  1:2033714/1‑99 (MQ=255)
                                                        cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAAAAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTAcccc                                            >  2:868853/1‑100 (MQ=255)
                                                         agcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCa                                           >  1:1540741/1‑100 (MQ=255)
                                                            agGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCa                                        <  1:1107894/100‑1 (MQ=255)
                                                            agGATACCCTTTTTAATGCCGCACACAGACGACCCCGTGACATACGGGGTCCTTCAGGCCATGGTATAACGTGTCATATTTCCTCGCTTCTACGCCAt                                          <  2:1797648/98‑1 (MQ=255)
                                                              gATATCGTATTTAATGCCGGACAAAAACGACCCCGTAATATACGGGGTCAATAAGGGCATGGTATAAAGGGTATTATTTCTTCTCTTCTACGCCATCAGt                                      <  2:2252851/100‑1 (MQ=255)
                                                               atatCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGtt                                     <  1:720826/100‑1 (MQ=255)
                                                                tatCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGttt                                    <  1:858618/100‑1 (MQ=255)
                                                                tatCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGttt                                    <  1:813136/100‑1 (MQ=255)
                                                                 atCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACAGGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTc                                   >  1:2216782/1‑100 (MQ=255)
                                                                  tCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCa                                  <  1:201361/100‑1 (MQ=255)
                                                                   cGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCag                                 >  1:1067464/1‑100 (MQ=255)
                                                                   cGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCag                                 >  1:1509314/1‑100 (MQ=255)
                                                                    gTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCaga                                >  1:1033648/1‑100 (MQ=255)
                                                                     tttttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCagag                               <  1:657743/100‑1 (MQ=255)
                                                                      ttttAATTCAGAACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGc                              <  2:255919/100‑1 (MQ=255)
                                                                       tttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCg                             <  2:1239332/100‑1 (MQ=255)
                                                                       tttAATTCCGCACAAAAACGACCCCGTAATATACGCGGTCACTAAGGACATGGCATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCg                             >  2:2120702/1‑100 (MQ=255)
                                                                         taatcacGCACAAAAACGACCCCGTCATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGa                            <  2:1885442/93‑1 (MQ=255)
                                                                              ccGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACTCCATCAGTTTCAGAGCGAATTaaa                      >  2:1254118/1‑100 (MQ=255)
                                                                              ccGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaa                      >  2:2148441/1‑100 (MQ=255)
                                                                               cGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaa                     >  2:493334/1‑100 (MQ=255)
                                                                               cGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACACCATCAGTTTCAGAGCGAATTaaaa                     >  2:1878578/1‑100 (MQ=255)
                                                                                 cacaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAt                   <  1:1278322/100‑1 (MQ=255)
                                                                                  acaAAAACGACCCAGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAACAAATTAAAAAtt                  <  2:1699285/100‑1 (MQ=255)
                                                                                    ttccgatctcccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtc                <  1:746763/91‑1 (MQ=255)
                                                                                    aaaaaaGACCGCGTAATATACGGGATCAATAAGGACAGGGTATAAAGTGTAGTATTTCTTCGCGTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtc                <  2:2310907/100‑1 (MQ=255)
                                                                                    aaaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtc                <  2:826766/100‑1 (MQ=255)
                                                                                      tattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtctc              >  2:1364238/5‑100 (MQ=255)
                                                                                      nattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtctc              >  2:1747601/5‑100 (MQ=255)
                                                                                      aaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtctc              >  1:622159/1‑100 (MQ=255)
                                                                                      aaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtct               >  2:842648/1‑99 (MQ=255)
                                                                                       attgACCCCGTATATTACGGGGTCGATAGGACATGGGATAAAGGGTATTATTTTTTCCGTTCTAAGCCATTCATTTCAAGGGGGATTTAAAAttt                 >  2:1691162/4‑95 (MQ=9)
                                                                                       attgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCa             >  2:2150270/4‑100 (MQ=255)
                                                                                          gACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgtt          >  2:187472/1‑100 (MQ=255)
                                                                                          gACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgtt          >  1:1283388/1‑100 (MQ=255)
                                                                                           aCCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTCCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgttg         >  2:964392/1‑100 (MQ=255)
                                                                                           aCCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgttg         >  1:2296278/1‑100 (MQ=255)
                                                                                            ccccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgttgt        >  1:1695551/1‑100 (MQ=255)
                                                                                             cccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtcagatcggaa       >  2:746761/1‑91 (MQ=255)
                                                                                              ccGTAATATACGGGGTCAATAAGGACATGGTATAAAGTGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGttt      >  2:1269088/1‑100 (MQ=255)
                                                                                                 tAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCg   <  2:997815/100‑1 (MQ=255)
                                                                                                  aaTATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCgg  <  2:1668869/100‑1 (MQ=255)
                                                                                                  aaTATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCgg  >  1:2323279/1‑100 (MQ=255)
                                                                                                  |                                                                                                    
GGGCGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGG  >  NC_000913/1314583‑1314780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: