Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,946,025 (A)7→6 100% intergenic (‑49/+90) ruvA ← / ← yobI component of RuvABC resolvasome, regulatory subunit/uncharacterized protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,946,0190A.98.7% 347.4 / ‑2.9 77intergenic (‑43/+96)ruvA/yobIcomponent of RuvABC resolvasome, regulatory subunit/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base . (40/36);  total (40/37)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGG  >  NC_000913/1945921‑1946114
                                                                                                  |                                                                                               
aTTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaa                                                                                                >  1:393861/1‑100 (MQ=255)
aTTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaa                                                                                                >  1:1776272/1‑100 (MQ=255)
aTTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaa                                                                                                >  2:1691699/1‑100 (MQ=255)
aTTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAACGCTGTATGATaa                                                                                                >  2:247281/1‑100 (MQ=255)
 ttAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaa                                                                                               >  2:1843585/1‑100 (MQ=255)
  tAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaa                                                                                              <  1:1913341/100‑1 (MQ=255)
   aaCACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaa                                                                                             >  2:708588/1‑100 (MQ=255)
     cacCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACCCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaaat                                                                                           >  2:1527315/1‑99 (MQ=255)
       ccAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaaatgc                                                                                         >  1:812160/1‑97 (MQ=255)
       ccAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaaatgc                                                                                         <  1:2388702/100‑4 (MQ=255)
       ccAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaaatgc                                                                                         >  1:1438177/1‑97 (MQ=255)
       ccAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaaatgc                                                                                         >  1:170269/1‑97 (MQ=255)
        cAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATaaaaaatgct                                                                                        <  1:2080860/100‑5 (MQ=255)
         aGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTg                                                                                      <  1:1712530/100‑1 (MQ=255)
         aGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTg                                                                                      <  2:384377/100‑1 (MQ=255)
          tcagtgtctgttTTTCAAGTATGTTGCCTCTAAATATGCCTATCACATTGCGATCCTACGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTgg                                                                                     <  2:2303195/92‑1 (MQ=255)
          gCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTgg                                                                                     >  2:1581059/1‑100 (MQ=255)
           cGGTGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTg                                                                                      <  2:1467056/98‑1 (MQ=255)
              gggTTGTTTTTCAATGAAGATGCCTCTGAGTCCGCCTATCACATGACGCTCCTGCGTAATGAATAAAAGATAATGCTGTAGGATAAAAAATGGTGgatag                                                                                 >  1:2248109/1‑100 (MQ=255)
               ggTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGgataga                                                                                >  2:2156104/1‑100 (MQ=255)
                gttgttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGgatagat                                                                               >  1:1204513/1‑100 (MQ=255)
                gttgttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGgatagat                                                                               >  1:365455/1‑100 (MQ=255)
                  tgttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGatat                                                                             >  1:884058/1‑100 (MQ=255)
                   gttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATc                                                                            >  2:1942571/1‑100 (MQ=255)
                   gttTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATc                                                                            >  1:961088/1‑100 (MQ=255)
                     ttttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCa                                                                          >  1:618924/1‑100 (MQ=255)
                      tttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCa                                                                          <  1:93117/99‑1 (MQ=255)
                      tttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAg                                                                         <  2:610952/100‑1 (MQ=255)
                      tttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAg                                                                         >  2:1639127/1‑100 (MQ=255)
                       ttCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCATGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAg                                                                         <  2:957407/99‑1 (MQ=255)
                         cAATGCTGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATATCTATCCAGcat                                                                      <  2:203855/100‑3 (MQ=255)
                         cAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGa                                                                      >  2:1131397/1‑100 (MQ=255)
                           atgatgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAATAAATGCTGGATAGATATCCAGCGAAg                                                                    <  1:1453628/100‑1 (MQ=255)
                           atgatgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATATCTATCCAGcatt                                                                     <  2:849977/99‑4 (MQ=255)
                               tgatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATg                                                                >  2:688070/1‑100 (MQ=255)
                                gatgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTACTGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGCAGGAt                                                                 >  2:1984415/1‑98 (MQ=255)
                                 atgCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATATCTATCCAGcattttttatc                                                              <  2:2272588/100‑11 (MQ=255)
                                   gCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATgaaga                                                            <  1:72662/100‑1 (MQ=255)
                                   gCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATgaaga                                                            >  1:1409282/1‑100 (MQ=255)
                                     ctctGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGaaa                                                          >  2:616450/1‑100 (MQ=255)
                                       ctGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAAc                                                        <  1:1659050/100‑1 (MQ=255)
                                         gAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACtt                                                      >  1:104549/1‑100 (MQ=255)
                                         gAGTCTGCCTATCACATGACGCTACTGCGTAATGAATCAAATAGAATGAAATAAGAAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAAc                                                        <  2:2075662/98‑1 (MQ=255)
                                            tttGCCTATCACATGACGCTTCTGCGTAATGAATCACAGATAATGCTGTATGATAAACAATGCTGGATAGCTTTCCAGCGATGGATGAAGAAAACTTGCg                                                   <  2:278355/98‑1 (MQ=255)
                                            tCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAGAACTTGCg                                                   <  2:497029/100‑1 (MQ=255)
                                            tCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAGAACTTGCg                                                   <  2:496370/100‑1 (MQ=255)
                                            tCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCg                                                   <  2:1921723/100‑1 (MQ=255)
                                             cTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGa                                                  >  2:560671/1‑100 (MQ=255)
                                               nccTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATACAGCGAAGGATGAAGAAAACTTGCGAg                                                 >  2:2350490/2‑99 (MQ=255)
                                               gCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAgg                                                >  2:2288806/1‑100 (MQ=255)
                                               gCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAgg                                                <  2:12863/100‑1 (MQ=255)
                                               gCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAgg                                                >  1:1780985/1‑100 (MQ=255)
                                               gCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAgg                                                >  1:1684505/1‑100 (MQ=255)
                                               gCCTATCACATGAAGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAg                                                 >  2:1472832/1‑99 (MQ=255)
                                               ccgatctacaTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAgg                                                <  2:594401/93‑1 (MQ=255)
                                               ccgatctacaTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAgg                                                <  2:594258/93‑1 (MQ=255)
                                                 cTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGgtg                                              >  1:729197/1‑100 (MQ=255)
                                                 cTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGgtg                                              <  1:560673/100‑1 (MQ=255)
                                                 cTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGgtg                                              <  2:334656/100‑1 (MQ=255)
                                                  tATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGgtgt                                             >  1:375226/1‑100 (MQ=255)
                                                  tATCACATGACGATCTTGCGTAATGAATCAAAGAGAATGCTGTATAATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGgtt                                              <  2:1051639/99‑2 (MQ=255)
                                                   aTCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtc                                            >  1:1340249/1‑100 (MQ=255)
                                                   aTCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtc                                            >  2:1530499/1‑100 (MQ=255)
                                                     cacaTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGtctc                                          >  1:1362087/1‑100 (MQ=255)
                                                      acaTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAggagatcgg                                         >  1:594260/1‑93 (MQ=255)
                                                      acaTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAggagatcgg                                         >  1:594403/1‑93 (MQ=255)
                                                      acaTGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCg                                         <  2:1776258/100‑1 (MQ=255)
                                                       caTGACGCTCCTGCGTGATGAATCAAAGATAATGCTGTATGATAAACAACGATGGATCGATATCCAGCGAAGGATGAAGAAACCTTGCGAGGTGTCTCGa                                        <  2:1388803/100‑1 (MQ=255)
                                                          gACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGa                                     <  1:1415967/100‑1 (MQ=255)
                                                            cGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAAc                                   <  2:1976342/100‑1 (MQ=255)
                                                             gcaccTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACt                                  <  2:1550834/97‑1 (MQ=255)
                                                               tacTGCGTAATGAACAAAAGAGAATGCTATAAGATAAAAAATGCTAGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGa                                <  2:686442/98‑1 (MQ=255)
                                                                   gCGTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGaaaaa                            >  2:912019/1‑100 (MQ=255)
                                                                     gTAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATg                          >  2:2129349/1‑100 (MQ=255)
                                                                      tAATGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATgg                         <  1:1340005/100‑1 (MQ=255)
                                                                       aatcaatcAAAGATACTACTGTATGATAATAAATGCTGGATAGATATACAGCGACGGAACAAGAAACCCTGCGAGGTGTCTCGCTGACCTGAAAAATGGc                        <  2:1772067/100‑1 (MQ=255)
                                                                       aaTGAATCAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGc                        >  1:454866/1‑100 (MQ=255)
                                                                              cAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGtat                 >  1:1505638/1‑100 (MQ=255)
                                                                              cAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGtat                 >  2:1966250/1‑100 (MQ=255)
                                                                              cAAAGATAATGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGtat                 >  1:2204290/1‑100 (MQ=255)
                                                                                   aTAATGCTGATTGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGACTCGATAAACTAAAAAATGGCACAGTATAAttt            <  2:1571378/100‑1 (MQ=255)
                                                                                     aaTGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTAt          <  1:2152531/100‑1 (MQ=255)
                                                                                      aTGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTAtt         <  1:556220/100‑1 (MQ=255)
                                                                                       tGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTTGATGAACTGAAAAATGGCACAGTATAATTTATTc        >  1:333012/1‑100 (MQ=255)
                                                                                       tGCTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTAtt         >  1:1500311/1‑99 (MQ=255)
                                                                                         cTGTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTa      >  2:975300/1‑100 (MQ=255)
                                                                                           gTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACa    >  2:1290048/1‑100 (MQ=255)
                                                                                           gTATGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACa    >  1:359317/1‑100 (MQ=255)
                                                                                             aTGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAgg  <  1:1014609/100‑1 (MQ=255)
                                                                                             aTGATAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTATCGATGAACTTAAAAATGGCACAGTATACTTTATTCTACatg  <  1:1609822/100‑3 (MQ=255)
                                                                                                  |                                                                                               
ATTAACACCAGCGGGGGTTGTTTTTCAATGATGATGCCTCTGAGTCTGCCTATCACATGACGCTCCTGCGTAATGAATCAAAGATAATGCTGTATGATAAAAAAATGCTGGATAGATATCCAGCGAAGGATGAAGAAAACTTGCGAGGTGTCTCGATGAACTGAAAAATGGCACAGTATAATTTATTCTACAGG  >  NC_000913/1945921‑1946114

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: