Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,018,260	(A)8→7	100%	coding (315/444 nt)	fliJ →	flagellar protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,018,253	0	A	.	98.5%	303.8 / ‑2.3	68	coding (308/444 nt)	fliJ	flagellar protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  major base . (34/33);  minor base T (0/1);  total (34/34)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAGATGG  >  NC_000913/2018154‑2018348                                                                                                    |                                                                                                agcagTTTATCCAGACGCTGGAAAACGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCgaga                                                                                                 >  2:740753/1‑100 (MQ=255) agcagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCgaga                                                                                                 >  2:1602425/1‑100 (MQ=255) agcagTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCgaga                                                                                                 >  1:758850/1‑100 (MQ=255)    agTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaa                                                                                               <  1:243626/99‑1 (MQ=255)     gTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaa                                                                                             >  1:1239872/1‑100 (MQ=255)        tATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaac                                                                                          >  1:138041/1‑99 (MQ=255)         aTCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaaca                                                                                         >  2:52237/1‑98 (MQ=255)          tCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaacaa                                                                                        <  2:861003/100‑4 (MQ=255)           ccAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGaaaaaaacaac                                                                                       >  2:2090978/1‑96 (MQ=255)              gACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGtt                                                                                   >  1:1365329/1‑100 (MQ=255)               aCGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGttt                                                                                  >  1:1872642/1‑100 (MQ=255)                cGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTg                                                                                 >  1:1272362/1‑100 (MQ=255)                  cTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCa                                                                               <  1:855611/100‑1 (MQ=255)                   tGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAg                                                                              >  2:1549253/1‑100 (MQ=255)                    ggAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAgg                                                                             >  1:155149/1‑100 (MQ=255)                         aaGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTg                                                                         <  2:232768/99‑1 (MQ=255)                           gCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCa                                                                      >  1:1758771/1‑100 (MQ=255)                            ccATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAg                                                                     <  1:866407/100‑1 (MQ=255)                             cATTACACAGCATCGCTAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCa                                                                      <  2:1871722/98‑1 (MQ=255)                                tACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGacac                                                                 <  1:1500823/100‑1 (MQ=255)                                  cTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTg                                                               >  2:591625/1‑100 (MQ=255)                                   tCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGc                                                              >  2:2108530/1‑100 (MQ=255)                                    cAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCa                                                             >  1:1242600/1‑100 (MQ=255)                                     aGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAg                                                            <  1:1530238/100‑1 (MQ=255)                                      gCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAgg                                                           <  1:1441866/100‑1 (MQ=255)                                          cGCCAGCAACCTAATCAGTGGACGCAGAAATGTGACTTTTCCATGAAAAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCTGACACTGCAGGAACg                                                       <  2:483378/100‑1 (MQ=255)                                           gCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACgg                                                      <  2:2091081/100‑1 (MQ=255)                                           gCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACg                                                       >  2:166123/1‑99 (MQ=255)                                            ccAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGc                                                     <  2:619688/100‑1 (MQ=255)                                             cAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCa                                                    <  1:383861/100‑1 (MQ=255)                                                cAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATc                                                 >  2:1324146/1‑100 (MQ=255)                                                 aaCTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATcc                                                <  1:327262/100‑1 (MQ=255)                                                   cTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAc                                              >  1:962190/1‑100 (MQ=255)                                                   cTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAc                                              >  2:191795/1‑100 (MQ=255)                                                    ttAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcg                                             <  1:2325734/100‑1 (MQ=255)                                                     tAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcgg                                            >  1:2060818/1‑100 (MQ=255)                                                     tAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcgg                                            >  1:332586/1‑100 (MQ=255)                                                      aaTCAGTGGACGCATAAAGTGGACATTGCGCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCTAcggc                                           <  2:1416984/100‑1 (MQ=255)                                                      aaTCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcggc                                           <  1:610672/100‑1 (MQ=255)                                                      aaTCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTAGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcggc                                           <  2:2144149/100‑1 (MQ=255)                                                       aTCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCAcggcg                                          <  1:591627/100‑1 (MQ=255)                                                         cAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACggcggc                                        >  2:902236/1‑100 (MQ=255)                                                         cAGGGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACggcggc                                        <  1:1052389/100‑1 (MQ=255)                                                          aGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCa                                       >  2:34813/1‑100 (MQ=255)                                                           gTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCAc                                      <  2:931186/100‑1 (MQ=255)                                                           gTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCATGGCAGACACTGAAGGAACGGCAATCCACGGCGGCAc                                      >  2:1111919/1‑100 (MQ=255)                                                            tGGACGCATAAAGTTGACATTGCCGTTAATAGTTTGCGA‑TAAAAAAACAACTTTTGCAGTCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACt                                     <  2:1893143/100‑1 (MQ=255)                                                              gACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGc                                   >  1:1827557/1‑100 (MQ=255)                                                               aCGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCt                                  >  2:2109025/1‑100 (MQ=255)                                                                cGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCtt                                 >  2:1922536/1‑100 (MQ=255)                                                                  cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               >  1:2052567/1‑100 (MQ=255)                                                                  cAGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGc                               >  2:1869779/1‑100 (MQ=255)                                                                   aGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGTAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCa                              <  1:1352682/100‑1 (MQ=255)                                                                   aGAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGAAGGAAAGGCAATCCACggcggcgc                                      >  2:2155739/1‑90 (MQ=255)                                                                    gAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAg                             <  1:52238/100‑1 (MQ=255)                                                                    gAAAGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAg                             <  1:325550/100‑1 (MQ=255)                                                                       aGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaaa                          <  1:1922552/100‑1 (MQ=255)                                                                       aGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaaa                          <  1:991136/100‑1 (MQ=255)                                                                       aGTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaaa                          >  1:210366/1‑100 (MQ=255)                                                                        gTTGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGaaaa                         >  1:2226482/1‑100 (MQ=255)                                                                          tGACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAAcc                       >  1:506413/1‑100 (MQ=255)                                                                           gACATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCg                      >  2:2317299/1‑100 (MQ=255)                                                                             cATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGcc                    >  1:2034964/1‑100 (MQ=255)                                                                             cATTGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGcc                    >  1:772273/1‑100 (MQ=255)                                                                               ttGCCCTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTc                  <  1:902238/100‑1 (MQ=255)                                                                                   ccTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAGTCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATc              <  2:1268775/100‑1 (MQ=255)                                                                                   ccTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATc              >  2:314143/1‑100 (MQ=255)                                                                                   ccTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATc              <  1:494573/100‑1 (MQ=255)                                                                                    cTGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCa             <  1:1230875/100‑1 (MQ=255)                                                                                     tGAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAg            <  2:283024/100‑1 (MQ=255)                                                                                      gAACAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGa           >  2:1768380/1‑100 (MQ=255)                                                                                       aaCAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGaa          >  1:2123123/1‑100 (MQ=255)                                                                                         cAGTTGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGaaaa        >  1:918266/1‑100 (MQ=255)                                                                                            ttGGCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAga     <  1:1339429/100‑1 (MQ=255)                                                                                              ggCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatg   <  2:840572/100‑1 (MQ=255)                                                                                               gCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatgg  <  2:2112842/100‑1 (MQ=255)                                                                                               gCGAG‑AAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAgatgg  <  2:983888/100‑1 (MQ=255)                                                                                                    |                                                                                                AGCAGTTTATCCAGACGCTGGAAAAAGCCATTACTCAGCATCGCCAGCAACTTAATCAGTGGACGCAGAAAGTTGACATTGCCCTGAACAGTTGGCGAGAAAAAAAACAACGTTTGCAGGCCTGGCAGACACTGCAGGAACGGCAATCCACGGCGGCACTGCTTGCAGAAAACCGCCTCGATCAGAAAAAGATGG  >  NC_000913/2018154‑2018348

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑