Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 2,531,596 (G)6→5 100% coding (134/762 nt) cysZ → sulfate transporter, sulfite inhibited

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,531,5910G.96.0% 216.2 / 2.0 50coding (129/762 nt)cysZsulfate transporter, sulfite inhibited
Reads supporting (aligned to +/- strand):  ref base G (1/1);  new base . (22/26);  total (23/27)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01

CAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATC  >  NC_000913/2531492‑2531685
                                                                                                   |                                                                                              
cAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATg                                                                                                <  2:1240940/100‑1 (MQ=255)
cAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATg                                                                                                <  1:1839285/100‑1 (MQ=255)
cAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTGTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATg                                                                                                >  2:1666315/1‑100 (MQ=255)
 aGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATgg                                                                                               <  2:2308615/100‑1 (MQ=255)
   cGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATgggg                                                                                             <  1:556467/100‑1 (MQ=255)
   cGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATgggg                                                                                             <  1:2308543/100‑1 (MQ=255)
        tttACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATgggggcgca                                                                                        >  2:423763/1‑96 (MQ=255)
           aCTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTc                                                                                    >  2:2200950/1‑100 (MQ=255)
                tttGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCtggtg                                                                               >  1:2258245/1‑100 (MQ=255)
                tttGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCtggtg                                                                               >  2:1240279/1‑100 (MQ=255)
                tttGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCtggtg                                                                               <  1:2200970/100‑1 (MQ=255)
                      cAAGGCTGGAAGCACGACTCGCGAACTGGGATTCGGTGCTTCGTTATTTTTCCGCTGCTGGTCAATATATTGTTGGTGGGGGCGCATCCTGGTGGctct                                                                          <  2:2104728/99‑1 (MQ=255)
                       aaGGCTGGAAGCTCGTCTCGCAAGCTGGGATTCGGCGTTTCGTTATTTTACCACTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCtt                                                                         <  2:1319655/99‑1 (MQ=255)
                         ggCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTac                                                                      <  2:1099318/100‑1 (MQ=255)
                         ggCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTac                                                                      >  2:559028/1‑100 (MQ=255)
                          gCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTaca                                                                     >  1:1395085/1‑100 (MQ=255)
                          gCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTaca                                                                     >  1:960223/1‑100 (MQ=255)
                          gCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTGTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTaca                                                                     <  2:1913398/100‑1 (MQ=255)
                            tGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTAcaca                                                                   <  2:1746919/100‑1 (MQ=255)
                             ggAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAg                                                                  >  2:1181595/1‑100 (MQ=255)
                                   tCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGAt                                                            <  2:2169866/100‑1 (MQ=255)
                                    cGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATg                                                           <  2:868974/100‑1 (MQ=255)
                                    cGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATg                                                           <  1:582440/100‑1 (MQ=255)
                                       ctcGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCt                                                        >  1:2212079/1‑100 (MQ=255)
                                            aaCCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATc                                                   >  1:946706/1‑100 (MQ=255)
                                              ccTGGGATTCGGCGTTACGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATccc                                                 <  2:1395077/100‑1 (MQ=255)
                                                tGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTATGGATCCCGa                                               >  1:187658/1‑100 (MQ=255)
                                                   gATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGActc                                            >  2:447090/1‑100 (MQ=255)
                                                      tCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCa                                         <  1:1626087/100‑1 (MQ=255)
                                                       cGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCAt                                        >  2:348356/1‑100 (MQ=255)
                                                        ggCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATg                                       <  2:946703/100‑1 (MQ=255)
                                                         gCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGa                                      <  2:1508820/100‑1 (MQ=255)
                                                          cGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGac                                     <  2:2270635/100‑2 (MQ=255)
                                                               cGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACg                                >  2:237263/1‑100 (MQ=255)
                                                                gTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGt                               <  2:659877/100‑1 (MQ=255)
                                                                  tATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTc                             <  2:483118/100‑1 (MQ=255)
                                                                    ttttACCGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCg                           <  1:1209740/100‑1 (MQ=255)
                                                                         ccGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTg                      <  1:824269/100‑1 (MQ=255)
                                                                         ccGCTGCTGGTCAATATTTTGTTGATGGGGGCGCATTCTGGGGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTg                      <  1:2069597/100‑1 (MQ=255)
                                                                           gctgctGGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGc                    >  2:1423550/1‑100 (MQ=255)
                                                                            ctgctgGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACCGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCt                   <  2:1783179/100‑1 (MQ=255)
                                                                              gctgGTCAATATTTTGTTGATGGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTa                  <  1:276551/100‑1 (MQ=255)
                                                                              gctgGTCAATATTTTGTTGATGGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTa                  >  1:1966383/1‑100 (MQ=255)
                                                                              gctgGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTAc                 >  1:901986/1‑100 (MQ=255)
                                                                              gctgGTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTAc                 <  1:359087/100‑1 (MQ=255)
                                                                               ctgGTCAATACTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTAc                 <  1:1512086/99‑1 (MQ=255)
                                                                                 ggTCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACa                >  2:835248/1‑98 (MQ=255)
                                                                                 ggTCAATATTTTGTTGATGGGGGCGAATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAAt              >  2:544031/1‑100 (MQ=255)
                                                                                   tCAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATgg            >  1:1957901/1‑100 (MQ=255)
                                                                                    cAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACTAAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACCTTCCGGACTGGCTACAATGGc           >  2:1113555/1‑100 (MQ=255)
                                                                                    cAATATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGc           <  1:348358/100‑1 (MQ=255)
                                                                                     aaTATTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCt          <  2:2069881/100‑1 (MQ=255)
                                                                                      atatTTTGTTGATGGGGGCGCATTCTAGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTg         >  1:1200440/1‑100 (MQ=255)
                                                                                        atTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAg       >  1:2156700/1‑100 (MQ=255)
                                                                                        atTTTGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAg       <  2:779630/100‑1 (MQ=255)
                                                                                         ttttGTTGATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGt      >  1:1407067/1‑100 (MQ=255)
                                                                                             gttgATGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATc  <  1:1420741/100‑1 (MQ=255)
                                                                                                   |                                                                                              
CAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATC  >  NC_000913/2531492‑2531685

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: