Predicted mutation | |||||||
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evidence | seq id | position | mutation | freq | annotation | gene | description |
RA | NC_000913 | 2,531,596 | (G)6→5 | 100% | coding (134/762 nt) | cysZ → | sulfate transporter, sulfite inhibited |
Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,531,591 | 0 | G | . | 96.0% | 216.2 / 2.0 | 50 | coding (129/762 nt) | cysZ | sulfate transporter, sulfite inhibited |
Reads supporting (aligned to +/- strand): ref base G (1/1); new base . (22/26); total (23/27) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.18e-01 |
CAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATC > NC_000913/2531492‑2531685 | cAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATg < 2:1240940/100‑1 (MQ=255) cAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATg < 1:1839285/100‑1 (MQ=255) cAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTGTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATg > 2:1666315/1‑100 (MQ=255) aGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATgg < 2:2308615/100‑1 (MQ=255) cGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATgggg < 1:556467/100‑1 (MQ=255) cGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATgggg < 1:2308543/100‑1 (MQ=255) tttACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATgggggcgca > 2:423763/1‑96 (MQ=255) aCTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTc > 2:2200950/1‑100 (MQ=255) tttGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCtggtg > 1:2258245/1‑100 (MQ=255) tttGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCtggtg > 2:1240279/1‑100 (MQ=255) tttGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCtggtg < 1:2200970/100‑1 (MQ=255) cAAGGCTGGAAGCACGACTCGCGAACTGGGATTCGGTGCTTCGTTATTTTTCCGCTGCTGGTCAATATATTGTTGGT‑GGGGGCGCATCCTGGTGGctct < 2:2104728/99‑1 (MQ=255) aaGGCTGGAAGCTCGTCTCGCAAGCTGGGATTCGGCGTTTCGTTATTTTACCACTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCtt < 2:1319655/99‑1 (MQ=255) ggCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTac < 2:1099318/100‑1 (MQ=255) ggCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTac > 2:559028/1‑100 (MQ=255) gCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTaca > 1:1395085/1‑100 (MQ=255) gCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTaca > 1:960223/1‑100 (MQ=255) gCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTGTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTaca < 2:1913398/100‑1 (MQ=255) tGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTAcaca < 2:1746919/100‑1 (MQ=255) ggAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAg > 2:1181595/1‑100 (MQ=255) tCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGAt < 2:2169866/100‑1 (MQ=255) cGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATg < 2:868974/100‑1 (MQ=255) cGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATg < 1:582440/100‑1 (MQ=255) ctcGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCt > 1:2212079/1‑100 (MQ=255) aaCCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATc > 1:946706/1‑100 (MQ=255) ccTGGGATTCGGCGTTACGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATccc < 2:1395077/100‑1 (MQ=255) tGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTATGGATCCCGa > 1:187658/1‑100 (MQ=255) gATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGActc > 2:447090/1‑100 (MQ=255) tCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCa < 1:1626087/100‑1 (MQ=255) cGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCAt > 2:348356/1‑100 (MQ=255) ggCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATg < 2:946703/100‑1 (MQ=255) gCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGa < 2:1508820/100‑1 (MQ=255) cGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGac < 2:2270635/100‑2 (MQ=255) cGTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACg > 2:237263/1‑100 (MQ=255) gTTATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGt < 2:659877/100‑1 (MQ=255) tATTTTACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTc < 2:483118/100‑1 (MQ=255) ttttACCGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCg < 1:1209740/100‑1 (MQ=255) ccGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTg < 1:824269/100‑1 (MQ=255) ccGCTGCTGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGGGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTg < 1:2069597/100‑1 (MQ=255) gctgctGGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGc > 2:1423550/1‑100 (MQ=255) ctgctgGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACCGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCt < 2:1783179/100‑1 (MQ=255) gctgGTCAATATTTTGTTGATGGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTa < 1:276551/100‑1 (MQ=255) gctgGTCAATATTTTGTTGATGGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTa > 1:1966383/1‑100 (MQ=255) gctgGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTAc > 1:901986/1‑100 (MQ=255) gctgGTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTAc < 1:359087/100‑1 (MQ=255) ctgGTCAATACTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTAc < 1:1512086/99‑1 (MQ=255) ggTCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACa > 2:835248/1‑98 (MQ=255) ggTCAATATTTTGTTGAT‑GGGGGCGAATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAAt > 2:544031/1‑100 (MQ=255) tCAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATgg > 1:1957901/1‑100 (MQ=255) cAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACTAAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACCTTCCGGACTGGCTACAATGGc > 2:1113555/1‑100 (MQ=255) cAATATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGc < 1:348358/100‑1 (MQ=255) aaTATTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCt < 2:2069881/100‑1 (MQ=255) atatTTTGTTGAT‑GGGGGCGCATTCTAGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTg > 1:1200440/1‑100 (MQ=255) atTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAg > 1:2156700/1‑100 (MQ=255) atTTTGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAg < 2:779630/100‑1 (MQ=255) ttttGTTGAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGt > 1:1407067/1‑100 (MQ=255) gttgAT‑GGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATc < 1:1420741/100‑1 (MQ=255) | CAGCGGTTTTTACTATTTTGCGCAAGGCTGGAAGCTCGTCTCGCAACCTGGGATTCGGCGTTTCGTTATTTTACCGCTGCTGGTCAATATTTTGTTGATGGGGGGCGCATTCTGGTGGCTCTTTACACAGCTCGATGTCTGGATCCCGACTCTCATGAGTTACGTTCCGGACTGGCTACAATGGCTGAGTTATC > NC_000913/2531492‑2531685 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |