Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 380,022 (G)10→9 100% intergenic (‑141/+47) frmR ← / ← yaiO regulator protein that represses frmRAB operon/outer membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913380,0130G.92.5% 325.5 / 16.8 80intergenic (‑132/+56)frmR/yaiOregulator protein that represses frmRAB operon/outer membrane protein
Reads supporting (aligned to +/- strand):  ref base G (2/3);  new base . (49/25);  total (52/28)
Fisher's exact test for biased strand distribution p-value = 3.40e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 106 total aligned reads displayed.

TAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTA  >  NC_000913/379914‑380111
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taggggggtatgcattgacatatagaatacccccctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAg                                                                                                    >  2:931360‑M2/46‑100 (MQ=255)
 aggggggtatgcattgacatatagaatacccccctatagtatatAACACTGGAGAATATAATTTATTCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                                                                                   >  2:676158‑M2/45‑100 (MQ=255)
 AGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                                                                                   <  2:2183558‑M1/100‑1 (MQ=255)
 AGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgg                                                                                                   <  1:1094043‑M1/100‑1 (MQ=255)
  ggggggtatgcattgacatatagaataccctcctatagtatatAACACTGGAGAATAAAATTTCTCCGCTGAATGTGGTCGGAAACCAAAGAGGAAAgg                                                                                                   >  2:43094‑M2/44‑99 (MQ=255)
  ggggggtatgcattgacatatagaatacccccctgtagtatatAAAACTGGAGAATAAAATTTATACGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                                                                                  >  2:932099‑M2/44‑100 (MQ=255)
  ggggggtatgcattgacatatagaatacccccctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggg                                                                                                  >  2:855479‑M2/44‑100 (MQ=255)
   gggggtatgcattgacatatagaatacccccctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                                                                                 >  1:146120‑M2/43‑100 (MQ=255)
   GGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                                                                                 <  2:1420250‑M1/100‑1 (MQ=255)
    GGGGTATTCTATATGTCAATGCATACCCCCCTATGGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAACAGGTAAggggg                                                                                                >  2:1249729‑M1/1‑100 (MQ=255)
    GGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggg                                                                                                >  2:1320767‑M1/1‑100 (MQ=255)
     GGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg                                                                                               >  2:772768‑M1/1‑100 (MQ=255)
     GGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggg                                                                                               <  1:1246006‑M1/100‑1 (MQ=255)
     GGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                                                                                 >  2:1781037‑M1/1‑98 (MQ=255)
      GGTATTCTATATGTCAATGTATACCCCCCTATAGTATATAACACAGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAggggggg                                                                                              >  2:1603313‑M1/1‑100 (MQ=255)
       gtatgcattgacatatagaataccgccctatagtatatAACACGGGAGAATAAAATTCATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggg                                                                                                 <  2:1464045‑M2/58‑1 (MQ=255)
       GTATTCTATATGGCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCAGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggg                                                                                             >  2:559087‑M1/1‑100 (MQ=255)
         ATTCTATCTGTAAATGCATACTCCCCTAGAGTATATAACACTGGAAAAAAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGACAgggggggg                                                                                             >  2:1536336‑M1/1‑98 (MQ=255)
         ATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAagggggggggc                                                                                           >  1:955410‑M1/1‑99 (MQ=255)
         ATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAagggggggggc                                                                                           >  1:2046975‑M1/1‑99 (MQ=255)
          TTCTATATGTCATTGCATACACCGCTATAGTAAATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAaaggggggggct                                                                                           >  2:786712‑M1/1‑97 (MQ=255)
            cTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAgggggggggctaa                                                                                        <  1:941433/100‑5 (MQ=255)
              atatGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATc                                                                                     >  2:959070/1‑100 (MQ=255)
                 tGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGc                                                                                  >  2:2263928/1‑100 (MQ=255)
                  gTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCa                                                                                 >  1:2230702/1‑100 (MQ=255)
                    cAATGCATACCCCGCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAgg                                                                               >  2:1472291/1‑100 (MQ=255)
                    cAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAgg                                                                               <  2:913448/100‑1 (MQ=255)
                       tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAggta                                                                             <  1:2209772/100‑3 (MQ=255)
                       tGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGa                                                                             >  2:896576/1‑100 (MQ=255)
                         cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAgg                                                                          >  1:1847640/1‑100 (MQ=255)
                         cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAgg                                                                          >  1:576730/1‑100 (MQ=255)
                         cATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAgg                                                                          <  1:1962956/100‑1 (MQ=255)
                           tacccccctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGgcc                                                                        >  1:985218‑M2/19‑100 (MQ=255)
                             cccccctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAGGAGGATAGGGGGGGGGCTAATCGGCAGGGAAGgccg                                                                       >  2:1603764‑M2/17‑99 (MQ=255)
                             cccccctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGgccgc                                                                      >  1:653052‑M2/17‑100 (MQ=255)
                             cccccctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGgccgcc                                                                     >  1:1538706‑M2/17‑100 (MQ=255)
                             cccccctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGATAATCGGCAGGGAAGgc                                                                         >  2:1529287‑M2/17‑96 (MQ=255)
                              ctcccatgagtatatAACACGGGAGAATAAAATTTATCCGGGGAAGGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAg                                                                           <  2:2345938‑M2/80‑1 (MQ=255)
                               cccctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGgccgcc                                                                     >  2:61127‑M2/15‑99 (MQ=255)
                               cccctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTAGGAAAACAAAGAGGAAAGGGGGGGGGGTAATAGGCAGGGAAgg                                                                          >  2:1065958‑M2/15‑94 (MQ=255)
                                 cctatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCgg                                                                 <  1:766400‑M2/88‑1 (MQ=255)
                                   tatagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGa                                                                <  2:2238177‑M2/90‑1 (MQ=255)
                                    atagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGgaagggcgccgggg                                                                 >  2:2271990‑M2/10‑94 (MQ=255)
                                    atagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCcgccccag                                                                 >  2:822788‑M2/10‑96 (MQ=255)
                                    atagtatatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGAt                                                               >  1:1030068‑M2/10‑100 (MQ=255)
                                      aGTATATAACACTGGAGAATAAAATTTAGCCGGTAAATGTGGCCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCATGGAAGGCCGCCCCGGATAg                                                             <  1:1038254/100‑1 (MQ=255)
                                        cttccgatcttGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcg                                                           <  1:341650/90‑1 (MQ=255)
                                         atatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCggg                                                         >  2:714659‑M2/5‑100 (MQ=255)
                                         atatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCGAATCGGCAGGGAAGGCCGCCCCGGATAGCggg                                                         >  1:1104392‑M2/5‑100 (MQ=255)
                                          tatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCggg                                                         >  2:2247530‑M2/4‑100 (MQ=255)
                                          tatAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCggg                                                         >  2:2043393‑M2/4‑100 (MQ=255)
                                           atAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGc                                                        >  1:87863‑M2/3‑100 (MQ=255)
                                           atAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGc                                                        <  1:559551‑M2/98‑1 (MQ=255)
                                           atAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATAGGCAGGGAAGGCCGCCCCGGATAGCggg                                                         >  1:103722‑M2/3‑99 (MQ=255)
                                             aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                     <  1:1814733/100‑1 (MQ=255)
                                             aaCACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                     <  1:918320/100‑1 (MQ=255)
                                              acacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                     <  1:1468032/100‑1 (MQ=255)
                                              acacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                     >  1:1507407/1‑100 (MQ=255)
                                              acacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                     <  2:1828370/100‑1 (MQ=255)
                                              acacTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGggcggc                                                     >  1:153522/1‑100 (MQ=255)
                                               cacTAGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCa                                                    <  2:2261400/100‑1 (MQ=255)
                                                  tGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAgcgagatcggaag                                                 >  2:341648/1‑90 (MQ=255)
                                                   ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                                >  1:2102268/1‑100 (MQ=255)
                                                   ggAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAg                                                <  1:5892/100‑1 (MQ=255)
                                                    gagaATAAAATTTATCCGGTGAATGTGGGCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAgg                                               <  2:2382784/100‑1 (MQ=255)
                                                        aTAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATc                                           >  1:1270884/1‑100 (MQ=255)
                                                          aaaaTTTATCGGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGaa                                             >  2:1680080/1‑96 (MQ=255)
                                                          aaaaTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGa                                        >  2:1489312/1‑100 (MQ=255)
                                                             aTTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGCAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAAGCAGAAt                                      >  1:1543704/1‑100 (MQ=255)
                                                              tttATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAAttt                                    <  2:2001150/100‑1 (MQ=255)
                                                                tATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTcc                                  <  1:2384881/100‑1 (MQ=255)
                                                                   ccGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAg                                >  1:1495789/1‑100 (MQ=255)
                                                                    cGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAgg                               <  1:2160835/100‑1 (MQ=255)
                                                                     ngTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt                              >  1:88674/2‑100 (MQ=255)
                                                                     ggTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGt                              <  1:1464054/100‑1 (MQ=255)
                                                                      gTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTc                             >  1:222491/1‑100 (MQ=255)
                                                                       tGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                            >  2:495637/1‑100 (MQ=255)
                                                                       tGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCa                            >  2:1117229/1‑100 (MQ=255)
                                                                        gAATGTGGTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGa                          >  1:897380/1‑100 (MQ=255)
                                                                        gAATGTGGTCGGAAAACAAAGAGGAACGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAg                           >  2:1770474/1‑100 (MQ=255)
                                                                          aTGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGAc                         >  1:35635/1‑100 (MQ=255)
                                                                              ggTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGc                     >  2:7214/1‑100 (MQ=255)
                                                                              ggTCGGAAAACAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCt                    >  2:570826/1‑100 (MQ=255)
                                                                               gTCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGc                     <  2:432630/99‑1 (MQ=255)
                                                                                tCGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTg                   >  1:2074642/1‑100 (MQ=255)
                                                                                 cGGAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGc                  >  2:1822725/1‑100 (MQ=255)
                                                                                 cGGAAAACAAAGAGGAAAGGGGGGGGGCTAACCGGCAGGAAAGGCCGCCCCTAATAGCGGGCGGCAGAATGAATCAGAATTTCCAGGTTAGACGGGCTGc                  <  2:508473/100‑1 (MQ=255)
                                                                                  ggAAAACAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCa                 >  2:1425601/1‑100 (MQ=255)
                                                                                    aaaaCAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAg               >  1:80044/1‑100 (MQ=255)
                                                                                     aaaCAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGt              <  1:1159660/100‑1 (MQ=255)
                                                                                        cAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCCGACGGTCTGCAACTTGc           >  2:840745/1‑100 (MQ=255)
                                                                                        cAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc           <  1:1859442/100‑1 (MQ=255)
                                                                                        cAAAGAGGAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc           <  1:959073/100‑1 (MQ=255)
                                                                                        cAAAGAGGAAAGAGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGc           >  1:1568806/1‑100 (MQ=255)
                                                                                        cAAAGAGGAAA‑‑GGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCa          <  2:1538695/100‑1 (MQ=255)
                                                                                              ggAAAGGGGGGGGGCTAATCGGCGGGGAAGCCCGCCACGAATAGCGAGCGGAAGAAGGAATCACGATTTACAGGTCAGACGCGCTGCAAGTTGCCGAc       >  2:420475/1‑98 (MQ=255)
                                                                                              ggAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCGCCGGATAGGGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg     >  2:217285/1‑100 (MQ=255)
                                                                                              ggAAAGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCg     >  1:723785/1‑100 (MQ=255)
                                                                                                  aGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTa  <  2:1884796/100‑1 (MQ=255)
                                                                                                   ggggggggggCTAATCGGCAGGGAAGGCCGCCCCGCATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGTCTGCAACTTGCAGCCCGt  <  2:1845419/98‑2 (MQ=255)
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TAGGGGGGTATTCTATATGTCAATGCATACCCCCCTATAGTATATAACACTGGAGAATAAAATTTATCCGGTGAATGTGGTCGGAAAACAAAGAGGAAAGGGGGGGGGGCTAATCGGCAGGGAAGGCCGCCCCGGATAGCGGGCGGCAGAAGGAATCAGAATTTCCAGGTCAGACGGGCTGCAAGTTGCAGACCGTTA  >  NC_000913/379914‑380111

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: