Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 3,277,706 (A)7→6 100% coding (370/465 nt) yhaV → toxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,277,7000A.93.8% 389.0 / 14.0 96coding (364/465 nt)yhaVtoxin of the SohB(PrlF)‑YhaV toxin‑antitoxin system
Reads supporting (aligned to +/- strand):  ref base A (1/3);  new base . (35/55);  total (37/59)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 112 total aligned reads displayed.

GTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACC  >  NC_000913/3277602‑3277795
                                                                                                  |                                                                                               
gTGCTTGTCGTTATCGTCACTTCTTGCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaa                                                                                                <  2:575341/100‑1 (MQ=255)
gTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaa                                                                                                >  1:1050801/1‑100 (MQ=255)
  gCTGGTCGTTATCGTCTCTTCTTTCGTTAGAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaa                                                                                              <  1:795742/100‑1 (MQ=255)
    tGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaa                                                                                            >  1:2364428/1‑100 (MQ=255)
    tGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaa                                                                                            >  1:1160437/1‑100 (MQ=255)
    tGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaa                                                                                            >  2:878341/1‑100 (MQ=255)
     ggTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaac                                                                                           >  2:1205519/1‑99 (MQ=255)
     ggTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaac                                                                                           <  1:1234695/100‑2 (MQ=255)
     ggTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaac                                                                                           <  2:1704777/100‑2 (MQ=255)
     ggTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaac                                                                                           <  1:1925979/100‑2 (MQ=255)
      gtggTTATCGTCGCTGCTTTTGTGATATTGAAAAAGAGAAAGTGTTCATTCTTTGATGGATGAACGATGAAAGCAGTTTGCGTATTTATGTTaaaaaa                                                                                            <  2:1893254/95‑1 (MQ=255)
      gTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaac                                                                                           <  1:801750/99‑2 (MQ=255)
      gTCGTTATCGGCTCTTCTTTCGTTATAGCGAAATAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaa                                                                                            <  2:1171278/98‑1 (MQ=255)
       tCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTaaaaaacag                                                                                         <  2:1509217/100‑4 (MQ=255)
          ttATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATg                                                                                     >  2:224606/1‑100 (MQ=255)
           tATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGc                                                                                    <  1:600527/100‑1 (MQ=255)
            aTCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGTGCACCTACGGTAAAAAACAGATGcc                                                                                   >  2:893316/1‑100 (MQ=255)
            aTCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGcn                                                                                   <  1:2186875/100‑2 (MQ=255)
            aTCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGcc                                                                                   <  1:1461664/100‑1 (MQ=255)
             tCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCt                                                                                  <  1:701104/100‑1 (MQ=255)
               gTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCtat                                                                                <  2:1664186/100‑1 (MQ=255)
               gTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCtat                                                                                >  1:48681/1‑100 (MQ=255)
                tctcTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCtata                                                                               <  1:957061/100‑1 (MQ=255)
                 ctctTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATAc                                                                              <  2:1227306/100‑1 (MQ=255)
                 ctctTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATAc                                                                              >  1:2275383/1‑100 (MQ=255)
                  tcttctTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATAcc                                                                             >  2:898180/1‑100 (MQ=255)
                  tcttctTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATAcc                                                                             <  2:1160431/100‑1 (MQ=255)
                  tcttctTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATAcc                                                                             <  2:2364407/100‑1 (MQ=255)
                    ttcttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGt                                                                           <  1:1529748/100‑1 (MQ=255)
                     tcttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCtan                                                                                <  2:964161/94‑2 (MQ=255)
                     tcttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTCGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGGAAAAAACAGATGCCTATACCGTa                                                                          >  2:1901519/1‑100 (MQ=255)
                      cttTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTAt                                                                         >  1:1084510/1‑100 (MQ=255)
                         tCGTTAGAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGAAAATAAAGAAAAAAACAGATGCCTATACCGTATTCa                                                                      <  2:19682/100‑1 (MQ=255)
                          cGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAg                                                                     >  1:1865144/1‑100 (MQ=255)
                           gTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAg                                                                     <  2:1840113/99‑1 (MQ=255)
                              ataGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCaaa                                                                 >  2:2363874/1‑100 (MQ=255)
                              aaagaaaaaaaGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGAAAAAAAAGTAAAAAACAGATGCCTATACCGTATTCa                                                                      <  2:194052/89‑1 (MQ=255)
                                aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAAt                                                               >  1:1537351/1‑100 (MQ=255)
                                aGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCACCaaaa                                                                >  2:1012301/1‑99 (MQ=255)
                                 gTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAAGACTCTGCGCACCTACGGTAAAACACAGATGCCTATACCGTATTCAGCAAAATg                                                              >  2:111081/1‑100 (MQ=255)
                                 gTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATg                                                              >  2:393950/1‑100 (MQ=255)
                                 gTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATg                                                              <  1:1079511/100‑1 (MQ=255)
                                  tGAAAAAGAGAAAGTCATCATACTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAAt                                                               >  2:2316168/1‑98 (MQ=255)
                                    aaaaaGAGAAATTCATCATTCTGGGATGGATGAACGATGAAAACACTCTTCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTa                                                           <  1:1389029/100‑1 (MQ=255)
                                        agagAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTaaaaa                                                       >  1:1143445/1‑100 (MQ=255)
                                        agagAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTaaaaa                                                       >  1:893867/1‑100 (MQ=255)
                                          agaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAaga                                                     <  2:160756/100‑1 (MQ=255)
                                           gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAagag                                                    <  1:1462523/100‑1 (MQ=255)
                                           gaAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAagag                                                    >  1:1499618/1‑100 (MQ=255)
                                            aaaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAgg                                                   <  1:995536/100‑1 (MQ=255)
                                            aaaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAgg                                                   <  1:2141546/100‑1 (MQ=255)
                                            aaaGTCATCATTCTGAGAAGGATGAACAATGAAAACACTCTGCGAAAAAAAAAAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAgg                                                   <  2:1899507/100‑1 (MQ=255)
                                            aaaGTAATCATTCTGAGATGAATGAACTATGAAAACACTCTGCGCAATAAAGTTAAAAAACAGATGCCTATCCCCGATTCAGCAAAATGTTAAAAagag                                                    <  2:1745787/99‑1 (MQ=255)
                                             aaGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGa                                                  <  1:1623819/100‑1 (MQ=255)
                                             aaGTCAACATTGTTGGAGGGAGGAACGTTGAAGCCACTGTGCTAACCAACGGTAAAAAACAGATGCCTATACCTTATTCAGCAAAGTGTTAAAAAGAGGa                                                  <  2:1400946/100‑1 (MQ=255)
                                             aaGTAATCATTCTGGGATGGATGAACAATGAAAACACTCTGAGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGa                                                  <  2:1622845/100‑1 (MQ=255)
                                                 catcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAt                                               >  2:1454454/1‑100 (MQ=255)
                                                 catcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtc                                              <  2:1143440/100‑1 (MQ=255)
                                                  atcatTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcc                                             <  1:2076093/100‑1 (MQ=255)
                                                    catTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAtcctc                                           >  2:1716211/1‑100 (MQ=255)
                                                    catTCTGAAATGAATGAACGATGAAAACACTCTGCGAAAAAAAAAAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACAt                                               <  2:1397871/96‑1 (MQ=255)
                                                       tCTGGGATGGATGAACGAAGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCGAGACCGTATTCAGCAAAATGTTAAAATGAGGACAtcctcc                                          <  2:1470400/98‑1 (MQ=255)
                                                        cTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAACATGTTAAAAAGAGGACATCCTCCTGc                                       >  2:1292223/1‑100 (MQ=255)
                                                            gatggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGAc                                   <  1:847090/100‑1 (MQ=255)
                                                              tggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTg                                 <  2:1891567/100‑1 (MQ=255)
                                                               ggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTgg                                <  2:1643145/100‑1 (MQ=255)
                                                               ggatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTg                                 <  1:2259397/99‑1 (MQ=255)
                                                                gatgAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTggg                               <  1:1948300/100‑1 (MQ=255)
                                                                   gAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaaa                            >  1:919086/1‑100 (MQ=255)
                                                                   gAACGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGaaa                            <  2:1148538/100‑1 (MQ=255)
                                                                    aaCGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAc                           >  1:1287064/1‑100 (MQ=255)
                                                                     aCGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAcc                          >  1:2083630/1‑100 (MQ=255)
                                                                     aCGATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAAcc                          <  1:1943629/100‑1 (MQ=255)
                                                                       gATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt                        <  1:744043/100‑1 (MQ=255)
                                                                       gATGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCt                        <  1:1815077/100‑1 (MQ=255)
                                                                         tGAAAACACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACCGGGAAACCCTCa                      >  1:1622934/1‑100 (MQ=255)
                                                                           aaaaCACTCTGCGCACTTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAcc                    >  2:1820535/1‑100 (MQ=255)
                                                                           aaaaCACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAcc                    <  1:572746/100‑1 (MQ=255)
                                                                           aaaaCACTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAcc                    >  2:1004217/1‑100 (MQ=255)
                                                                            aaaCACTCTGCGCACCTACGGTAAAAAACAGATGCCTACACCGTATTAAGAAAAAAGTCAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCAccc                   <  2:1298210/100‑1 (MQ=255)
                                                                              acacTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCga                 <  1:568347/100‑1 (MQ=255)
                                                                              acacTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCga                 >  1:465186/1‑100 (MQ=255)
                                                                                acTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCTTCCTGCCGACTGGGAAACCCTCACCCgaga               >  1:2184785/1‑100 (MQ=255)
                                                                                acTCTGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCgaga               <  2:683527/100‑1 (MQ=255)
                                                                                 ctctGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACGCTCACCCGAGaa              <  1:1564643/100‑1 (MQ=255)
                                                                                 ctctGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCgag                <  2:2306275/98‑1 (MQ=255)
                                                                                 ctctGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGaa              <  2:87114/100‑1 (MQ=255)
                                                                                 ctctGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGaa              <  2:1199886/100‑1 (MQ=255)
                                                                                   ctGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAAc            >  1:1140689/1‑100 (MQ=255)
                                                                                    tGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACa           <  2:494943/100‑1 (MQ=255)
                                                                                    tGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACa           <  2:639534/100‑1 (MQ=255)
                                                                                    tGCGCACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACa           >  2:1559583/1‑100 (MQ=255)
                                                                                     gcgcACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACag          <  1:2080783/100‑1 (MQ=255)
                                                                                      cgcACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACaga         >  2:2061359/1‑100 (MQ=255)
                                                                                       gcACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACaga         <  2:1050797/99‑1 (MQ=255)
                                                                                        cACCTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACagaag       <  1:831018/100‑1 (MQ=255)
                                                                                          ccTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAgaagaa     <  1:524368/100‑1 (MQ=255)
                                                                                           cTACGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGaaa    <  2:1506011/100‑1 (MQ=255)
                                                                                             aCGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAcc  <  1:2303475/100‑1 (MQ=255)
                                                                                             aCGGTAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAAc   <  2:856701/99‑1 (MQ=255)
                                                                                                  |                                                                                               
GTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCATTCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGTTAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACC  >  NC_000913/3277602‑3277795

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: