BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	4,494,883:1	(A)_7→8	100%	coding (261/564 nt)	idnK →	D‑gluconate kinase, thermosensitive

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,494,876	1	.	A	91.5%	299.1 / 20.1	94	coding (254/564 nt)	idnK	D‑gluconate kinase, thermosensitive
Reads supporting (aligned to +/- strand): ref base . (1/7); new base A (43/43); total (44/50)
Fisher's exact test for biased strand distribution p-value = 6.31e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 119 total aligned reads displayed.

TATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATG  >  NC_000913/4494777‑4494970

tATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑t‑t                                                                                                     <  1:1751287/100‑1 (MQ=255)

tATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTAATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑t‑t                                                                                                     >  2:2377009/1‑100 (MQ=255)

 aTCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑a                                                                                                  <  2:1511522/100‑1 (MQ=255)

 aTCTGATGAAGATAGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑t‑t                                                                                                     <  2:862960/99‑1 (MQ=255)

   cTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑aaa                                                                                                >  1:1652454/1‑100 (MQ=255)

    tgatgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑aaa‑a                                                                                              >  2:220445/1‑100 (MQ=255)

       tgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑aaa‑a‑aaa                                                                                          >  1:366162/1‑100 (MQ=255)

       tgaAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑aaa‑a‑a                                                                                            >  1:855053/1‑98 (MQ=255)

         aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑aaa‑a‑aaaac                                                                                        <  1:620881/100‑3 (MQ=255)

         aaGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑aaa‑a‑aaaac                                                                                        >  2:1559875/1‑98 (MQ=255)

          aGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTTTCTGTTCATCA‑T‑T‑‑aaa‑a‑aaaaca                                                                                       >  2:1843088/1‑97 (MQ=255)

          aGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGCCTGTTCATCA‑T‑T‑‑aaa‑a‑aaaaca                                                                                       >  1:94745/1‑97 (MQ=255)

           gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑aaa‑a‑aaaacag                                                                                      <  1:63817/100‑5 (MQ=255)

           gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑aaa‑a‑aaaacag                                                                                      <  1:2341003/100‑5 (MQ=255)

           gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑aaa‑a‑aaaacag                                                                                      >  2:123277/1‑96 (MQ=255)

           gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑aaa‑a‑aaaacag                                                                                      >  2:2364159/1‑96 (MQ=255)

           gATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑AAA‑A‑AAA‑CAg                                                                                      <  1:2243256/99‑1 (MQ=255)

            aTCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑aaa‑a‑aaaacagt                                                                                     >  2:506492/1‑95 (MQ=255)

            aTCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑aaa‑a‑aaaacagt                                                                                     <  1:953851/100‑6 (MQ=255)

            aTCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑aaa‑a‑aaaacagt                                                                                     >  2:1202978/1‑95 (MQ=255)

               gACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATc                                                                                  >  2:729837/1‑100 (MQ=255)

                atttCCCTGGCTGCAACGCTTAAATGATGCTACATACAGTCTATATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCg                                                                                 >  1:1612561/3‑100 (MQ=255)

                aCTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCg                                                                                 <  1:189323/100‑1 (MQ=255)

                 cTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTa                                                                                    >  1:61953/1‑96 (MQ=255)

                   tCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAACGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCg                                                                                 >  1:1804349/1‑97 (MQ=255)

                   tCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGa                                                                              <  1:1553162/100‑1 (MQ=255)

                    cccTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGat                                                                             >  2:2132943/1‑100 (MQ=255)

                          ctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATAtttt                                                                        >  2:2101734/1‑99 (MQ=255)

                          ctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTa                                                                       >  1:2333975/1‑100 (MQ=255)

                          ctggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTa                                                                       >  1:2334060/1‑100 (MQ=255)

                           tggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTAc                                                                      <  1:1469940/100‑1 (MQ=255)

                            ggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACg                                                                     <  1:1649203/100‑1 (MQ=255)

                            ggAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑AAA‑AGAAA‑CAGTATCGTGATATTTTACg                                                                     <  1:352465/100‑1 (MQ=255)

                              aaCGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCa                                                                   <  1:461646/100‑1 (MQ=255)

                                 gattAAATGATGCTTTATACGGTCTTTATAAAAAGAATGAGACAGGATTTATTGTCTGAAAAAAA‑A‑AA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAgg                                                                <  2:1320648/98‑1 (MQ=255)

                                  cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAggg                                                               >  1:1993920/1‑100 (MQ=255)

                                  cTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGGCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGGATCGTGATATTTTACGCAAgg                                                                >  2:14971/1‑99 (MQ=255)

                                   ttAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TAAAAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAggg                                                               <  1:328585/100‑1 (MQ=255)

                                    tAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTa                                                             >  1:938814/1‑100 (MQ=255)

                                    tAAATGAGGCTACATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTa                                                             >  2:1253931/1‑100 (MQ=255)

                                      aaTGATGGTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTTTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAggg                                                               <  2:2330802/96‑1 (MQ=255)

                                          atgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTTTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAgggcacctccc                                                       >  1:1074957/1‑92 (MQ=255)

                                           tgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCa                                                      <  1:668239/100‑1 (MQ=255)

                                           tgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCa                                                      <  2:413427/100‑1 (MQ=255)

                                           tgCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCa                                                      <  1:1274099/100‑1 (MQ=255)

                                            tcTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTCTTGTCTGTTCATCA‑T‑T‑‑AAA‑A‑AAACCAGTATCGTGATATTTTACGCAAGGGTAGCCCCCAt                                                     <  2:1671200/99‑1 (MQ=255)

                                              ttCATACAGTATTTATAAAAAGAATGAAACAGGATTTGTTGTCAATTAAACA‑A‑AA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATg                                                    <  2:1705830/99‑1 (MQ=255)

                                               tCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCAtaga                                                  >  1:1671213/1‑97 (MQ=255)

                                               tCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGtt                                                  <  2:549514/100‑1 (MQ=255)

                                                cATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTc                                                 >  2:1957602/1‑100 (MQ=255)

                                                cATACAGTCTTTATAAAAAGAATGAAACAGGATGTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTc                                                 >  2:1428414/1‑100 (MQ=255)

                                                 ttACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCa                                                <  2:106101/99‑1 (MQ=255)

                                                  tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTTTGTTCATAA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAt                                               >  2:1475687/1‑100 (MQ=255)

                                                  tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCa                                                >  1:106102/1‑99 (MQ=255)

                                                  tACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCACCATGTTCAt                                               >  2:1265491/1‑100 (MQ=255)

                                                    cAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCAttt                                             >  1:1486092/1‑100 (MQ=255)

                                                      gtgtggatgaaaaGAAGGATGGAGGTTTTATTGTGTGGTGATCAGT‑A‑‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCTCATGTTCATTTc                                            <  2:1926744/90‑1 (MQ=255)

                                                        cTTTATAAAAAGAATGAAACAGGCTTTATTGTCTGTTCATCA‑T‑T‑‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctct                                        >  2:881018/1‑100 (MQ=255)

                                                        cTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctc                                         >  2:1236140/1‑100 (MQ=255)

                                                        cTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctc                                         >  2:1123059/1‑100 (MQ=255)

                                                        cTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTAAAAA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCctc                                         <  1:2328267/100‑1 (MQ=255)

                                                          ttATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTg                                       <  1:1698098/100‑1 (MQ=255)

                                                           tataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTgg                                      <  2:870865/100‑1 (MQ=255)

                                                           tataAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTgg                                      >  2:1663145/1‑100 (MQ=255)

                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                    <  1:1719739/100‑1 (MQ=255)

                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                    <  1:1719976/100‑1 (MQ=255)

                                                             taAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGtt                                    <  1:1900302/100‑1 (MQ=255)

                                                              aaaaaGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTa                                   >  1:832351/1‑100 (MQ=255)

                                                               aaaaGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TAAAAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTa                                   >  2:1770033/1‑100 (MQ=255)

                                                               aaaaGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAg                                  >  1:446361/1‑100 (MQ=255)

                                                                aaaGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGa                                 >  2:759863/1‑100 (MQ=255)

                                                                aaaGAATGAAACAGGATTTATTATCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGa                                 <  2:405662/100‑1 (MQ=255)

                                                                aaaGAATAAAACAGGATTTATTGTATGTTCATCA‑T‑T‑‑AAA‑AGAAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATATCCTCTGGTTa                                   <  2:832741/98‑1 (MQ=255)

                                                                   gAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATgg                              >  2:653511/1‑100 (MQ=255)

                                                                   gAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATgg                              <  1:1091829/100‑1 (MQ=255)

                                                                   gAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATgg                              <  2:1840643/100‑1 (MQ=255)

                                                                     aTGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCg                            <  1:1593361/100‑1 (MQ=255)

                                                                     aTGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCg                            <  1:1209942/100‑1 (MQ=255)

                                                                     aTGAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCg                            >  1:213630/1‑100 (MQ=255)

                                                                       gAAACAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGa                           >  2:672035/1‑99 (MQ=255)

                                                                         aaCAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTa                        <  1:698994/100‑1 (MQ=255)

                                                                          aCAGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTAt                       >  1:2375404/1‑100 (MQ=255)

                                                                            aGGATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGa                     <  1:978062/100‑1 (MQ=255)

                                                                              gATTTATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGaa                    <  2:527743/99‑1 (MQ=255)

                                                                                tttATTGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACt                 >  2:1146521/1‑100 (MQ=255)

                                                                                     tGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtct            <  1:1902875/100‑1 (MQ=255)

                                                                                     tGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtct            >  2:706341/1‑100 (MQ=255)

                                                                                     tGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtct            <  1:1554671/100‑1 (MQ=255)

                                                                                     tGTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtc             >  1:1542263/1‑99 (MQ=255)

                                                                                      gTCTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATtctc           <  1:826213/100‑1 (MQ=255)

                                                                                       tCTGTTAAGCA‑T‑T‑‑AAAGA‑AAT‑GAGTATCGTGATATTTTATGCAAGGGTGGCACATATGTTACATTCGTGTGGTTAGATGGCGACTATGAAACTATtctc           <  2:1074953/99‑1 (MQ=255)

                                                                                        cTGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgc         <  1:1833216/100‑1 (MQ=255)

                                                                                         tGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcg        >  1:76790/1‑100 (MQ=255)

                                                                                         tGTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcg        <  2:1238045/100‑1 (MQ=255)

                                                                                          gTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcgc       >  2:1475827/1‑100 (MQ=255)

                                                                                          gTTCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTcgcg        >  2:81072/1‑99 (MQ=255)

                                                                                           ttCATCA‑TAG‑‑AAG‑A‑AAA‑CAGTATCGTGATATTTTACGCAGGGGGAGGCGCCAGGTGCATTTCCTCTGGTTAGGTGGCGACTATGAAGGTATTCTcgcgcc      <  1:2377030/100‑2 (MQ=255)

                                                                                           ttCATCA‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTTTcgcgcg      <  1:1253938/100‑1 (MQ=255)

                                                                                            tcatca‑T‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGa     <  1:985374/100‑1 (MQ=255)

                                                                                               tca‑t‑TA‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATg  >  2:1958650/1‑100 (MQ=255)

TATCTGATGAAGATCGACTTCCCTGGCTGGAACGCTTAAATGATGCTTCATACAGTCTTTATAAAAAGAATGAAACAGGATTTATTGTCTGTTCATCA‑T‑T‑‑AAA‑A‑AAA‑CAGTATCGTGATATTTTACGCAAGGGTAGCCCCCATGTTCATTTCCTCTGGTTAGATGGCGACTATGAAACTATTCTCGCGCGAATG  >  NC_000913/4494777‑4494970

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 12 ≤` `ATCG/ATCG` `< 27 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG` `< 41 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`