Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_000913 1,314,685:1 +T 15.2% intergenic (+27/+33) ompW → / ← yciE outer membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,314,6841.T15.2% 287.3 / 57.3 92intergenic (+26/+34)ompW/yciEouter membrane protein W/putative rubrerythrin/ferritin‑like metal‑binding protein
Reads supporting (aligned to +/- strand):  ref base . (43/35);  new base T (8/6);  total (51/41)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.69e-02

CGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAAT  >  NC_000913/1314586‑1314783
                                                                                                   |                                                                                                   
cGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAatat                                                                                                    <  1:1703182/100‑1 (MQ=255)
    ggagaggaaCGCGATAGCGTACGCCGCGAGCCGTGGGTGGGTATGTTCGCAGCAGGATATCGTTTTTGATTCCGCACAAAAACGACCCCGTAATATACgg                                                                                                <  2:2239815/93‑1 (MQ=255)
     cacaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACggg                                                                                               <  1:784685/100‑1 (MQ=255)
      acaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgggg                                                                                              <  2:2054070/100‑1 (MQ=255)
      acaGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACggg                                                                                               <  1:389149/99‑1 (MQ=255)
       caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                             >  1:2245037/1‑100 (MQ=255)
       caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                             <  1:1128457/100‑1 (MQ=255)
       caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGt                                                                                             >  2:523625/1‑100 (MQ=255)
       caGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGt                                                                                             >  1:303687/1‑100 (MQ=255)
        aGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTc                                                                                            <  1:1039255/100‑1 (MQ=255)
         gCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACgggg                                                                                              >  2:185143/1‑97 (MQ=255)
         gCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcg                                                                                           <  2:303685/100‑2 (MQ=255)
         gCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcg                                                                                           <  2:1838060/100‑2 (MQ=255)
           aaCACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGCACCCGTAATATACGGGGTCAAt                                                                                         >  2:460272/1‑100 (MQ=255)
           aaCACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcgtt                                                                                         <  1:772618/100‑4 (MQ=255)
           aaCACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcgtt                                                                                         <  2:1495628/100‑4 (MQ=255)
              acGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAg                                                                                      <  1:830445/100‑1 (MQ=255)
              acGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAg                                                                                      >  2:1130107/1‑100 (MQ=255)
              acGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGATCAATAAg                                                                                      <  1:472348/100‑1 (MQ=255)
               cGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAgg                                                                                     <  2:1874243/100‑1 (MQ=255)
               cGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAgg                                                                                     >  1:1339949/1‑100 (MQ=255)
               cGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcgtttttg                                                                                     <  1:885607/100‑8 (MQ=38)
               cGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGAAGGATATCGTTTTTAATTCAGCACAAAAACGACCCCGTAATATACGGGGGAAATaa                                                                                       >  1:38381/1‑98 (MQ=255)
                gttagcccaCGCCTCGATCCCTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGa                                                                                    <  2:1613930/92‑1 (MQ=255)
                 aTAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTATATTACGGGGTcgtttttgtg                                                                                   <  1:2360256/100‑10 (MQ=37)
                    gCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATg                                                                                <  2:18921/100‑1 (MQ=255)
                     cGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATgg                                                                               <  1:44725/100‑1 (MQ=255)
                      gTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGt                                                                              >  2:93845/1‑100 (MQ=255)
                       tACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGta                                                                             <  2:18384/100‑1 (MQ=255)
                          gCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGAACAAAAACGACCACGTAATGTACGGGGTCAATAAGGAAATGGTATaa                                                                          >  2:1943015/1‑100 (MQ=255)
                           ccTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaaa                                                                         >  1:1668882/1‑100 (MQ=255)
                           ccTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaaa                                                                         >  2:1855218/1‑100 (MQ=255)
                           ccTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaaa                                                                         >  1:2312858/1‑100 (MQ=255)
                           ccTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATaaa                                                                         >  2:950564/1‑100 (MQ=255)
                            cTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTAAATACCGCACAAAAACGATCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAg                                                                        >  2:1294103/1‑100 (MQ=255)
                                   cGTGGGTGTTTATGTTCTCAGCAGGGTATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatta                                                                 >  1:871416/1‑100 (MQ=255)
                                   cGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatta                                                                 >  1:471991/1‑100 (MQ=255)
                                   cGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtatta                                                                 >  1:505923/1‑100 (MQ=255)
                                   cGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTACAAAGGGtatta                                                                 >  1:2267788/1‑100 (MQ=255)
                                    gTGGGTGTTTATGTTCTCAGGAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGtat                                                                   <  2:1954691/97‑1 (MQ=255)
                                      gggTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTAttt                                                              >  1:1834483/1‑100 (MQ=255)
                                      gggTGTGTATATTCTCAACAGGATATCGTTTTTAATTCCGCACAAAAACGACCCAGTAATATACGGGGTCAATAAGGACATCGTAGAAAAGCtatt                                                                  >  2:3667/1‑96 (MQ=255)
                                       ggTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATttc                                                             >  1:840766/1‑100 (MQ=255)
                                          gtTTATGGTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTAAATAAGGACATGGTATAAAGGGTATTATttcttc                                                          <  1:1339926/100‑1 (MQ=255)
                                             tATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCt                                                       >  1:376717/1‑100 (MQ=255)
                                             tATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCt                                                       >  1:1853901/1‑100 (MQ=255)
                                              aTGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCtt                                                      <  2:1820282/100‑1 (MQ=255)
                                                  tctcAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTAc                                                  <  1:1282892/100‑1 (MQ=255)
                                                   ctcAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACg                                                 >  1:913641/1‑100 (MQ=255)
                                                     cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGc                                                >  1:2033714/1‑99 (MQ=255)
                                                     cagcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAAAAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTAcccc                                               >  2:868853/1‑100 (MQ=255)
                                                      agcagGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCa                                              >  1:1540741/1‑100 (MQ=255)
                                                         agGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCa                                           <  1:1107894/100‑1 (MQ=255)
                                                         agGATACCCTTTTTAATGCCGCACACAGACGACCCCGTGACATACGGGGTCCTTCAGGCCATGGTATAACGTGTCATATTTCCTCGCTTCTACGCCAt                                             <  2:1797648/98‑1 (MQ=255)
                                                           gATATCGTATTTAATGCCGGACAAAAACGACCCCGTAATATACGGGGTCAATAAGGGCATGGTATAAAGGGTATTATTTCTTCTCTTCTACGCCATCAGt                                         <  2:2252851/100‑1 (MQ=255)
                                                            atatCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGtt                                        <  1:720826/100‑1 (MQ=255)
                                                             tatCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGttt                                       <  1:858618/100‑1 (MQ=255)
                                                             tatCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGttt                                       <  1:813136/100‑1 (MQ=255)
                                                              atCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACAGGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTc                                      >  1:2216782/1‑100 (MQ=255)
                                                               tCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCa                                     <  1:201361/100‑1 (MQ=255)
                                                                cGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCag                                    >  1:1509314/1‑100 (MQ=255)
                                                                cGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCag                                    >  1:1067464/1‑100 (MQ=255)
                                                                 gTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCaga                                   >  1:1033648/1‑100 (MQ=255)
                                                                  tttttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCagag                                  <  1:657743/100‑1 (MQ=255)
                                                                   ttttAATTCAGAACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGc                                 <  2:255919/100‑1 (MQ=255)
                                                                    tttAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCg                                <  2:1239332/100‑1 (MQ=255)
                                                                    tttAATTCCGCACAAAAACGACCCCGTAATATACGCGGTCACTAAGGACATGGCATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCg                                >  2:2120702/1‑100 (MQ=255)
                                                                      taatcacGCACAAAAACGACCCCGTCATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGa                               <  2:1885442/93‑1 (MQ=255)
                                                                           ccGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACTCCATCAGTTTCAGAGCGAATTaaa                         >  2:1254118/1‑100 (MQ=255)
                                                                           ccGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaa                         >  2:2148441/1‑100 (MQ=255)
                                                                            cGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTaaaa                        >  2:493334/1‑100 (MQ=255)
                                                                            cGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACACCATCAGTTTCAGAGCGAATTaaaa                        >  2:1878578/1‑100 (MQ=255)
                                                                              cacaAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAAt                      <  1:1278322/100‑1 (MQ=255)
                                                                               acaAAAACGACCCAGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAACAAATTAAAAAtt                     <  2:1699285/100‑1 (MQ=255)
                                                                                 ttccgatctcccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtc                   <  1:746763/91‑1 (MQ=255)
                                                                                 aaaaaaGACCGCGTAATATACGGGATCAATAAGGACAGGGTATAAAGTGTAGTATTTCTTCGCGTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtc                   <  2:2310907/100‑1 (MQ=255)
                                                                                 aaaaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtc                   <  2:826766/100‑1 (MQ=255)
                                                                                   tattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtctc                 >  2:1364238/5‑100 (MQ=255)
                                                                                   nattgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtctc                 >  2:1747601/5‑100 (MQ=255)
                                                                                   aaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtctc                 >  1:622159/1‑100 (MQ=255)
                                                                                   aaaCGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtct                  >  2:842648/1‑99 (MQ=255)
                                                                                    attgACCCCGTATATTACGGGGTCGATAGGACATGGGATAAAGGGTATTATTTTTTCCGTTCTAAGCCATTCATTTCAAGGGGGATTTAAAAttt                    >  2:1691162/4‑95 (MQ=9)
                                                                                    attgACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCa                >  2:2150270/4‑100 (MQ=255)
                                                                                       gACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgtt             >  1:1283388/1‑100 (MQ=255)
                                                                                       gACCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgtt             >  2:187472/1‑100 (MQ=255)
                                                                                        aCCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTCCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgttg            >  2:964392/1‑100 (MQ=255)
                                                                                        aCCCCGTATATTACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgttg            >  1:2296278/1‑100 (MQ=255)
                                                                                         ccccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAgttgt           >  1:1695551/1‑100 (MQ=255)
                                                                                          cccGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTtcagatcggaa          >  2:746761/1‑91 (MQ=255)
                                                                                           ccGTAATATACGGGGTCAATAAGGACATGGTATAAAGTGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGttt         >  2:1269088/1‑100 (MQ=255)
                                                                                              tAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCg      <  2:997815/100‑1 (MQ=255)
                                                                                               aaTATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCgg     <  2:1668869/100‑1 (MQ=255)
                                                                                               aaTATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCgg     >  1:2323279/1‑100 (MQ=255)
                                                                                                  ataCGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAat  <  1:2230518/100‑1 (MQ=255)
                                                                                                   |                                                                                                   
CGGTGCACAGCAACACGATAGCGTACGCCTCGATCCGTGGGTGTTTATGTTCTCAGCAGGATATCGTTTTTAATTCCGCACAAAAACGACCCCGTAATATACGGGGTCAATAAGGACATGGTATAAAGGGTATTATTTCTTCGCTTCTACGCCATCAGTTTCAGAGCGAATTAAAAATTTCTCAGTTGTTTGCGGAAT  >  NC_000913/1314586‑1314783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: