Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_000913	2,462,889:1	(C)9→10	100%	intergenic (+243/‑123)	fadL → / → yfdF	long‑chain fatty acid outer membrane transporter/uncharacterized protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,462,880	1	.	C	67.8%	190.5 / 108.5	87	intergenic (+234/‑132)	fadL/yfdF	long‑chain fatty acid outer membrane transporter/uncharacterized protein
Reads supporting (aligned to +/- strand):  ref base . (15/13);  new base C (28/30);  total (44/43)
Fisher's exact test for biased strand distribution p-value = 8.18e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATA  >  NC_000913/2462785‑2462976                                                                                                    |                                                                                                      gCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑c‑ccc                                                                                                 >  2:1742463/1‑100 (MQ=255)   ggTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑c‑ccc‑c‑c                                                                                             >  2:418938/1‑100 (MQ=255)      aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑c‑ccc‑c‑c‑ccc                                                                                         <  1:967124/100‑1 (MQ=255)      aTCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑c‑ccc‑c‑c‑cc                                                                                          <  1:66433/99‑1 (MQ=255)        ncacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑c‑ccc‑c‑c‑cccct                                                                                       >  2:238216/2‑98 (MQ=255)        ccACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑c‑ccc‑c‑c‑ccccc                                                                                       >  1:412168/1‑98 (MQ=255)         cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑c‑ccc‑c‑c‑cccctg                                                                                      <  2:1307598/100‑4 (MQ=255)         cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑c‑ccc‑c‑c‑cccctg                                                                                      >  1:151838/1‑97 (MQ=255)         cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑c‑ccc‑c‑c‑cccctg                                                                                      >  1:1655055/1‑97 (MQ=255)         cacaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑A‑‑c‑ccc‑c                                                                                               >  1:606525/1‑93 (MQ=255)          acaGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGc                                                                                     <  1:2337582/100‑1 (MQ=255)            aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCt                                                                                    <  2:216471/100‑1 (MQ=255)            aGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CTC‑C‑C‑GCCTGGCt                                                                                    <  2:1246633/99‑1 (MQ=255)             gACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCCGC‑C‑CCCTgg                                                                                      >  2:2032398/1‑97 (MQ=255)                aaaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATtg                                                                                <  1:1410909/100‑1 (MQ=255)                 aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGCGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATtg                                                                                <  2:703524/99‑1 (MQ=255)                 aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTTTtgt                                                                               >  2:1765716/1‑100 (MQ=255)                 aaCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATtgt                                                                               <  1:928151/100‑1 (MQ=255)                  aCCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑CAACCTGGCTGTtggg                                                                              >  1:241237/1‑98 (MQ=255)                   ccTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATtgtg                                                                              >  1:2129319/1‑99 (MQ=255)                    cTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTgcg                                                                            >  1:1767199/1‑100 (MQ=255)                    cTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TA‑C‑CCC‑C‑C‑CCCTGGCTATTGGgc                                                                             >  2:1512961/1‑99 (MQ=255)                      gAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCt                                                                          <  1:914963/100‑1 (MQ=255)                      gAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCt                                                                          <  1:991478/100‑1 (MQ=255)                        aGGGAAAGGGGTAGGTTCAGGGGTTCTGCTCGTCCTTCTCAAGGAGTGTACTTTTGTGCAGTG‑CCGGGAT‑GC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCGCa                                                                        <  2:1123578/100‑1 (MQ=255)                        aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTc                                                                         <  2:2215273/99‑1 (MQ=255)                        aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCa                                                                        >  1:1870/1‑100 (MQ=255)                        aGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCa                                                                        <  2:1811383/100‑1 (MQ=255)                         gggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCAt                                                                       <  2:1864537/100‑1 (MQ=255)                         gggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATa                                                                      >  1:1959238/1‑100 (MQ=255)                          ggAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATa                                                                      >  1:1284017/1‑100 (MQ=255)                           gAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATa                                                                      >  2:1703055/1‑99 (MQ=255)                           gAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATaca                                                                    >  1:948285/1‑100 (MQ=255)                           gAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATaca                                                                    >  1:1236757/1‑100 (MQ=255)                               ggCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTTTTGTGCGCTCTTAAACt                                                                  >  2:193156/1‑99 (MQ=255)                                 cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTTTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTTTTTTGCGCTCATACACt                                                                  >  2:917271/1‑97 (MQ=255)                                 cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACCCaa                                                               >  1:1433896/1‑100 (MQ=255)                                 cATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑CAC‑CCCTGGCTATTGTGCGCCCATACACTCaa                                                               >  2:2704/1‑100 (MQ=255)                                   tatTTTCTGGCTTTCTCCCCTTCTTTCTCAAACAGTTT‑CTTTTTTTCATTTCCCAGGTTCCC‑C‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCa                                                                <  2:519300/97‑1 (MQ=255)                                    atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAAtt                                                            >  1:917570/1‑100 (MQ=255)                                    atTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTAGTGTGCGCTCATACACCCCAAt                                                             >  1:326630/1‑99 (MQ=255)                                     ttttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTa                                                           <  2:496091/100‑1 (MQ=255)                                      tttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTaa                                                          <  2:2040833/100‑1 (MQ=255)                                      tgtctggCGTACTACTCGACCTTCTCAAACAATTTATTTTTCTGCATTT‑CCAGTCT‑AC‑C‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGATCATACCCTCAAAt                                                             <  2:493585/91‑1 (MQ=255)                                       ttCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTaaa                                                         <  2:2356501/100‑1 (MQ=255)                                         ccggCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAg                                                        <  1:1133155/97‑1 (MQ=255)                                          aGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCACATTAAAGAt                                                      >  2:2189943/1‑100 (MQ=255)                                            gCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                   >  2:2352734/1‑100 (MQ=255)                                             cGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                   <  1:755596/100‑1 (MQ=255)                                             cGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                   <  1:2032416/100‑1 (MQ=255)                                             cGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAgg                                                   <  1:289985/100‑1 (MQ=255)                                              gTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGt                                                  >  1:2339670/1‑100 (MQ=255)                                               ttCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCCGGAT‑AC‑TCCC‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGt                                                  <  2:412166/100‑1 (MQ=255)                                                  tGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑ACTT‑‑C‑CCC‑C‑C‑CCCTCGCTATTGTGCGCTCATACACTCACATTAAAGAAAGGTTCta                                              >  1:1733792/1‑100 (MQ=255)                                                    cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaa                                            <  1:22693/100‑1 (MQ=255)                                                    cTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTCCGCTCATACACTCAAATTAAAAATAGGTTCtaaa                                            >  1:52217/1‑100 (MQ=255)                                                     tCGTCCTTCTCAAAGAGTTTACTTTTTTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaat                                           <  2:1333772/100‑1 (MQ=255)                                                      cGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaataa                                         >  2:1894728/1‑100 (MQ=255)                                                       gTCCTTCTCAAAGAGTTTACTTTCCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCtaaataa                                         <  1:2290056/100‑1 (MQ=255)                                                            tctcAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCCCTTGTACCCCCATACACTCAAATTAAAGATAGGTTAtaaataca                                        >  2:173811/1‑94 (MQ=255)                                                             ctcAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGc                                   >  1:355783/1‑100 (MQ=255)                                                              tcAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCg                                  <  2:2143497/100‑1 (MQ=255)                                                              tcAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCg                                  <  2:939958/100‑1 (MQ=255)                                                               cAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtt                                >  1:369832/1‑100 (MQ=255)                                                               cAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGAGtt                                >  1:1180733/1‑100 (MQ=255)                                                                aaaGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttt                               >  1:386356/1‑100 (MQ=255)                                                                 aaGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttt                               <  1:2082276/100‑1 (MQ=255)                                                                 aaGAGTTTACTTTTCTGCATTT‑CCAGAAT‑AA‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGttt                               <  2:2105663/100‑1 (MQ=255)                                                                  agagTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TCCC‑CCC‑C‑C‑CCCTGGCTATTTTTCGCCCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCtttt                               >  2:1714683/1‑100 (MQ=255)                                                                  agagTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGtttt                              <  1:65093/100‑1 (MQ=255)                                                                  agagCTTCCTTTTCTGCATTA‑CCAGTAT‑AC‑T‑‑CGCCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAACATAGATTCTAAATAAATGAGCGttt                               <  2:12663/99‑1 (MQ=255)                                                                     gTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTCTTGTGCCCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTg                           >  1:275759/1‑100 (MQ=255)                                                                      tttACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                         <  2:1180727/100‑1 (MQ=255)                                                                       ttACTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGAt                         <  1:1742477/100‑1 (MQ=255)                                                                          cTTTTCTGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGAAAGGTTCTAAATAACTGAGCGTTTTTTGATAGt                      >  1:1710791/1‑100 (MQ=255)                                                                          cTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGAGTTTTTTCATAGTc                     >  1:2361302/1‑100 (MQ=255)                                                                          cTTTTCGGCATTA‑CCAGGAT‑AC‑T‑‑C‑CCCGC‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAg                       <  2:276449/99‑1 (MQ=255)                                                                               cTGCATTT‑CCAGGAT‑AC‑TCCC‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCCAATTAAAGATCGGTTCCAAATAAATCACCGTTTTTTGATAGTCTa                   >  1:557967/1‑99 (MQ=255)                                                                               cTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAttt                <  1:651763/100‑1 (MQ=255)                                                                                tGCATTT‑CCCGAAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGACTCTTGTGCGCCCATACACTCAAAATAAAAATAAGTTCAACATAAATAAAAGTTTTATGATAATc                     >  2:1594097/1‑95 (MQ=255)                                                                                tGCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAACGTTTTTTGATAGTCTAttt                >  1:1318544/1‑100 (MQ=255)                                                                                 gCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTAttt                >  1:51814/1‑99 (MQ=255)                                                                                 gCATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTc               <  2:151837/100‑1 (MQ=255)                                                                                  cATTT‑CCCGAAT‑AC‑A‑‑C‑CCC‑C‑CACCCTGGCTATTGTGCGCTCATACCCTCAAATTAAACATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa              <  2:792028/100‑1 (MQ=255)                                                                                  cATTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCa              >  1:2166489/1‑100 (MQ=255)                                                                                   aTTT‑CCAGGAT‑AC‑TCCC‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTc               <  1:1015272/99‑1 (MQ=255)                                                                                   aTTT‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCAt             >  1:1575600/1‑100 (MQ=255)                                                                                      t‑CCAGGAT‑ACGC‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTa           <  1:195731/99‑1 (MQ=255)                                                                                      t‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAg          <  1:237200/100‑1 (MQ=255)                                                                                      t‑CCAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAg          >  2:876610/1‑100 (MQ=255)                                                                                      t‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAg          <  2:2299749/99‑1 (MQ=255)                                                                                        ccAGGAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAgg         >  1:2213409/1‑100 (MQ=255)                                                                                        ccAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGt        >  1:1553546/1‑100 (MQ=255)                                                                                         cAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTa       >  2:2306403/1‑100 (MQ=255)                                                                                           ggAT‑AC‑TC‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGTTTTTTTTTTTCTTTATGTaa      >  2:1099623/1‑100 (MQ=255)                                                                                              t‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatata  <  1:323957/100‑1 (MQ=255)                                                                                              t‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAatata  <  2:772716/100‑1 (MQ=255)                                                                                                    |                                                                                                      GCGGTATCCACAGACAAACCTGAAGGGAAAGGCATATTTTCAGGCGTTCTGCTCGTCCTTCTCAAAGAGTTTACTTTTCTGCATTT‑CCAGGAT‑AC‑T‑‑C‑CCC‑C‑C‑CCCTGGCTATTGTGCGCTCATACACTCAAATTAAAGATAGGTTCTAAATAAATGAGCGTTTTTTGATAGTCTATTTCATTAGGTAATATA  >  NC_000913/2462785‑2462976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑